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The map label for this gene is fruR [H]

Identifier: 162148442

GI number: 162148442

Start: 2754647

End: 2755687

Strand: Direct

Name: fruR [H]

Synonym: GDI_2661

Alternate gene names: 162148442

Gene position: 2754647-2755687 (Clockwise)

Preceding gene: 162148437

Following gene: 162148449

Centisome position: 69.84

GC content: 64.94

Gene sequence:

>1041_bases
ATGAAACCCGCCGTTCCCGCCATGGACCGACCCGACAGCGACCCGCCATCCATCGCGCAGGTGGCCAAGGCCACCAACTG
CTCGGTCACCACGGTCCGGCTCGTCCTGAACGGACAGGCTGGAAAATACCGGATCAGCGCCGCCACGCAGAAGATCGTTC
GCGATTATGTCGATCTGCATGGGTATTGCCTCAACCATGCGGCGCGAAGCCTCAAGCTGCGCCGGACCGATACGATCGGC
CTGGTCGTGCCGGAGATCGCCAATCCGTTCTTTGCCGGGCTGATGGCCTGCCTGGAACGATTGTGCGCGGCCCGCGGACT
GGTGCTGCTGACCGCGTCGTCCGGCGAAATGCCGGCGCAGGAGGACCGCGCGATCAGGGAACTGGGCACCCGTGGGATCG
ATGCCCTGATCGTCGTACCATGCCAGGCGCCCCGCTACGGGCGCCTTCTTGGCGGCGCGAAGGACATTCCGGTGGTCCTG
CTCGATCGCGCCTATACGGGCGCCCCCTATACCGTCATCGCCAGCGACAATTTCGGCGAATGCGTTGCCCTGACCGATGC
GATCCTCGATGCCGGGGAGCAGCCGCATTTCATCTGCGCGAATGAACGCCTGCCGAGCATCGGCGATAGAATCGCGGGAT
TCGGCGCGGCATGCCGGAAACGACGCATCCGGAACTGGCGCGCCCGGGTCCATTGCGCGGATCAGGACAGCATCCACAGC
GCGGGAACGATCGTCGACGCGCTGCTGGCGCAGGGCGGCCCACCCCGTGCCCTGATCTGCTCCTCCCTCCTGATGCTGCA
AGGGACCATGGAGCGCCTGAAGCTCCGTCTCGGGCAGTTGCCGCCGGATATCACCTTCGGAACATTCGACGATCATTTCA
TGCTCGATTTTCTGCCGAACCACACATTTGCCATTCGCCAGGACGCGGACATGCTGGCCGCACTGGCGCTGGACTATCTC
GATCAGCATCCGACCGGACCCGACGGTCCCGGCCGTGTGCTTGCCCCCTGCACGTTCGTTCAACGCCCCGCGAGGCGATA
G

Upstream 100 bases:

>100_bases
TTTGCTAAGTCGTGTTAGCACACTATTAGCGTGTATCGTTCCGTGCAAGGACGATATCGGCCGCCCTGTGCATCTTCGCA
AAACTGTGACGAAAGAACCG

Downstream 100 bases:

>100_bases
GCCCGGGCGGCCGTCCCGCCTTCGATCGCGGCGCGTGCCTCGCCTCCGACCGAGGCCAGAAGCGCGTCGCTTGCGTCACG
CCACGCGTCATAGCCGCCTG

Product: fructose repressor

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKPAVPAMDRPDSDPPSIAQVAKATNCSVTTVRLVLNGQAGKYRISAATQKIVRDYVDLHGYCLNHAARSLKLRRTDTIG
LVVPEIANPFFAGLMACLERLCAARGLVLLTASSGEMPAQEDRAIRELGTRGIDALIVVPCQAPRYGRLLGGAKDIPVVL
LDRAYTGAPYTVIASDNFGECVALTDAILDAGEQPHFICANERLPSIGDRIAGFGAACRKRRIRNWRARVHCADQDSIHS
AGTIVDALLAQGGPPRALICSSLLMLQGTMERLKLRLGQLPPDITFGTFDDHFMLDFLPNHTFAIRQDADMLAALALDYL
DQHPTGPDGPGRVLAPCTFVQRPARR

Sequences:

>Translated_346_residues
MKPAVPAMDRPDSDPPSIAQVAKATNCSVTTVRLVLNGQAGKYRISAATQKIVRDYVDLHGYCLNHAARSLKLRRTDTIG
LVVPEIANPFFAGLMACLERLCAARGLVLLTASSGEMPAQEDRAIRELGTRGIDALIVVPCQAPRYGRLLGGAKDIPVVL
LDRAYTGAPYTVIASDNFGECVALTDAILDAGEQPHFICANERLPSIGDRIAGFGAACRKRRIRNWRARVHCADQDSIHS
AGTIVDALLAQGGPPRALICSSLLMLQGTMERLKLRLGQLPPDITFGTFDDHFMLDFLPNHTFAIRQDADMLAALALDYL
DQHPTGPDGPGRVLAPCTFVQRPARR
>Mature_346_residues
MKPAVPAMDRPDSDPPSIAQVAKATNCSVTTVRLVLNGQAGKYRISAATQKIVRDYVDLHGYCLNHAARSLKLRRTDTIG
LVVPEIANPFFAGLMACLERLCAARGLVLLTASSGEMPAQEDRAIRELGTRGIDALIVVPCQAPRYGRLLGGAKDIPVVL
LDRAYTGAPYTVIASDNFGECVALTDAILDAGEQPHFICANERLPSIGDRIAGFGAACRKRRIRNWRARVHCADQDSIHS
AGTIVDALLAQGGPPRALICSSLLMLQGTMERLKLRLGQLPPDITFGTFDDHFMLDFLPNHTFAIRQDADMLAALALDYL
DQHPTGPDGPGRVLAPCTFVQRPARR

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=326, Percent_Identity=31.5950920245399, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1789202, Length=277, Percent_Identity=26.7148014440433, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790194, Length=315, Percent_Identity=24.7619047619048, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=23.9520958083832, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787948, Length=312, Percent_Identity=24.0384615384615, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787906, Length=206, Percent_Identity=27.6699029126214, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37457; Mature: 37457

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAVPAMDRPDSDPPSIAQVAKATNCSVTTVRLVLNGQAGKYRISAATQKIVRDYVDLH
CCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHH
GYCLNHAARSLKLRRTDTIGLVVPEIANPFFAGLMACLERLCAARGLVLLTASSGEMPAQ
HHHHHHHHHHEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCH
EDRAIRELGTRGIDALIVVPCQAPRYGRLLGGAKDIPVVLLDRAYTGAPYTVIASDNFGE
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCC
CVALTDAILDAGEQPHFICANERLPSIGDRIAGFGAACRKRRIRNWRARVHCADQDSIHS
HHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHH
AGTIVDALLAQGGPPRALICSSLLMLQGTMERLKLRLGQLPPDITFGTFDDHFMLDFLPN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCC
HTFAIRQDADMLAALALDYLDQHPTGPDGPGRVLAPCTFVQRPARR
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHCCCCCC
>Mature Secondary Structure
MKPAVPAMDRPDSDPPSIAQVAKATNCSVTTVRLVLNGQAGKYRISAATQKIVRDYVDLH
CCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHH
GYCLNHAARSLKLRRTDTIGLVVPEIANPFFAGLMACLERLCAARGLVLLTASSGEMPAQ
HHHHHHHHHHEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCH
EDRAIRELGTRGIDALIVVPCQAPRYGRLLGGAKDIPVVLLDRAYTGAPYTVIASDNFGE
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCC
CVALTDAILDAGEQPHFICANERLPSIGDRIAGFGAACRKRRIRNWRARVHCADQDSIHS
HHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHH
AGTIVDALLAQGGPPRALICSSLLMLQGTMERLKLRLGQLPPDITFGTFDDHFMLDFLPN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCC
HTFAIRQDADMLAALALDYLDQHPTGPDGPGRVLAPCTFVQRPARR
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]