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The map label for this gene is potD [H]

Identifier: 162148373

GI number: 162148373

Start: 2684189

End: 2685283

Strand: Reverse

Name: potD [H]

Synonym: GDI_2591

Alternate gene names: 162148373

Gene position: 2685283-2684189 (Counterclockwise)

Preceding gene: 162148375

Following gene: 162148372

Centisome position: 68.08

GC content: 65.66

Gene sequence:

>1095_bases
ATGCGGCAGTGGACATCGCGACGGCCTGGCAGAACGGGAATTGCGCGCGCCTGTCCCGGTTTCCGAATCATCCTGCCTGC
GATCCTGATCGTTTTCGGCTGCCAGGCATCATCGGCGGCACCGTTGAATTTCCTGGTCTGGGAATCGGGCGTCAGTCCCG
AAGTGGTCAAGTCGTGGACCGACATGACCGGCATCGACATCAACCAGATCATCTTCGACAGTGGGGACCGGCGCGACCTG
ATGGTCGCGAACGTGAATGCGCCGATCGACCTGGCGGTAATCGACAGCGCCCACGTCCAGGCGTTCGGCGAACGCGGGCT
CCTGCTCGACCAGTCCCGCTTCCCCCTGCCGCCGGAGGAGCAGAGCGAAGGGCGGTGGCGCGCGATGTGCGGCCGTTACG
GCGTGCCCTATAGCTGGGGGAATACCGGCATCGTCTACCGCGCCGACCGGCTGAAGGTGCCGCCCAGTTCGTGGGGCGAC
CTGCTGAAGCCCGCCCCGGCGCTGGTGGGACATATCGCGATGCCCGATGATCAGGAGGAACTGCTGGCTGCCGCCCTGAA
TTATCTGGGGCGTCCGATGAACAGCGGCGATCCCCAGGACCTGCGCCGCGCGTTCGACCTGCTGCGCGCCCAGGCGCCGG
CCGTCCTGATCTATGGATTGCCCGTCACCGCGCAGCAGGAACGCAGCGTGCATGACCTGATCGATATCGGACTGGGGGGA
ACGGGGGACGAGAAGACCCTGAACATGACGACGGACCAGACCCAGCCCTGGCGGTTCGTCACGCCGCGTGAAGGGGTGAT
GGTATGGGTCGACTGCCTGGCTGTGCTCAGCCGGACCTCCCGTCCCGACCTGGCGGAGAAGTTCATCCGCTTCCTGGTCA
GGCCGGACAATGCGGTCCGGGATGCGATCTTCCTGCACCAGCCGACACCGAATCCGCGCGCCGAGGCGATGCTGCCCCGC
GCGGTCCGCGATGATCCCCAGATCTATCCGCCCCAATCCTACCCCATCATCTATCGCAAGCCCGTGCCGACCGACAGCCT
GGCGTTGCGGAGGCGGATTCTCGGCGCCCTGATTGCGATCCATGACGCTCAATAG

Upstream 100 bases:

>100_bases
TCGGTCATATCGTCCAGGAGTCTTGCACTATACTCGCGCGACGGCCTGTCGCCGCTATTGGGACAAAGGGTTGGCCGGAA
TGGAGGGGCTGTTTTCCGGC

Downstream 100 bases:

>100_bases
ACGCGCATTCGCGGTCCTGCTGCCGATCGTCCTGCTGGGCCACATCCTGGCCGGCGGGGCGGCGTTCCTGGGATACCGTA
CCGCACTGGTCCTGCTGCAC

Product: spermidine/putrescine-binding periplasmic protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: Norspermidine-binding protein [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MRQWTSRRPGRTGIARACPGFRIILPAILIVFGCQASSAAPLNFLVWESGVSPEVVKSWTDMTGIDINQIIFDSGDRRDL
MVANVNAPIDLAVIDSAHVQAFGERGLLLDQSRFPLPPEEQSEGRWRAMCGRYGVPYSWGNTGIVYRADRLKVPPSSWGD
LLKPAPALVGHIAMPDDQEELLAAALNYLGRPMNSGDPQDLRRAFDLLRAQAPAVLIYGLPVTAQQERSVHDLIDIGLGG
TGDEKTLNMTTDQTQPWRFVTPREGVMVWVDCLAVLSRTSRPDLAEKFIRFLVRPDNAVRDAIFLHQPTPNPRAEAMLPR
AVRDDPQIYPPQSYPIIYRKPVPTDSLALRRRILGALIAIHDAQ

Sequences:

>Translated_364_residues
MRQWTSRRPGRTGIARACPGFRIILPAILIVFGCQASSAAPLNFLVWESGVSPEVVKSWTDMTGIDINQIIFDSGDRRDL
MVANVNAPIDLAVIDSAHVQAFGERGLLLDQSRFPLPPEEQSEGRWRAMCGRYGVPYSWGNTGIVYRADRLKVPPSSWGD
LLKPAPALVGHIAMPDDQEELLAAALNYLGRPMNSGDPQDLRRAFDLLRAQAPAVLIYGLPVTAQQERSVHDLIDIGLGG
TGDEKTLNMTTDQTQPWRFVTPREGVMVWVDCLAVLSRTSRPDLAEKFIRFLVRPDNAVRDAIFLHQPTPNPRAEAMLPR
AVRDDPQIYPPQSYPIIYRKPVPTDSLALRRRILGALIAIHDAQ
>Mature_364_residues
MRQWTSRRPGRTGIARACPGFRIILPAILIVFGCQASSAAPLNFLVWESGVSPEVVKSWTDMTGIDINQIIFDSGDRRDL
MVANVNAPIDLAVIDSAHVQAFGERGLLLDQSRFPLPPEEQSEGRWRAMCGRYGVPYSWGNTGIVYRADRLKVPPSSWGD
LLKPAPALVGHIAMPDDQEELLAAALNYLGRPMNSGDPQDLRRAFDLLRAQAPAVLIYGLPVTAQQERSVHDLIDIGLGG
TGDEKTLNMTTDQTQPWRFVTPREGVMVWVDCLAVLSRTSRPDLAEKFIRFLVRPDNAVRDAIFLHQPTPNPRAEAMLPR
AVRDDPQIYPPQSYPIIYRKPVPTDSLALRRRILGALIAIHDAQ

Specific function: Acts as a sensor of norspermidine and enhances biofilm formation. When complexed to norspermidine, could interact with the periplasmic portion of mbaA to regulate its enzymatic activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=325, Percent_Identity=25.8461538461538, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787367, Length=321, Percent_Identity=27.1028037383178, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40454; Mature: 40454

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQWTSRRPGRTGIARACPGFRIILPAILIVFGCQASSAAPLNFLVWESGVSPEVVKSWT
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
DMTGIDINQIIFDSGDRRDLMVANVNAPIDLAVIDSAHVQAFGERGLLLDQSRFPLPPEE
CCCCCCHHHHEECCCCCCEEEEEECCCCEEEEEECCHHHHHHCCCCEEECCCCCCCCCCC
QSEGRWRAMCGRYGVPYSWGNTGIVYRADRLKVPPSSWGDLLKPAPALVGHIAMPDDQEE
CCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCHHHHEECCCCCCHHH
LLAAALNYLGRPMNSGDPQDLRRAFDLLRAQAPAVLIYGLPVTAQQERSVHDLIDIGLGG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
TGDEKTLNMTTDQTQPWRFVTPREGVMVWVDCLAVLSRTSRPDLAEKFIRFLVRPDNAVR
CCCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
DAIFLHQPTPNPRAEAMLPRAVRDDPQIYPPQSYPIIYRKPVPTDSLALRRRILGALIAI
CEEEEECCCCCCHHHHHCCHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHE
HDAQ
ECCC
>Mature Secondary Structure
MRQWTSRRPGRTGIARACPGFRIILPAILIVFGCQASSAAPLNFLVWESGVSPEVVKSWT
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
DMTGIDINQIIFDSGDRRDLMVANVNAPIDLAVIDSAHVQAFGERGLLLDQSRFPLPPEE
CCCCCCHHHHEECCCCCCEEEEEECCCCEEEEEECCHHHHHHCCCCEEECCCCCCCCCCC
QSEGRWRAMCGRYGVPYSWGNTGIVYRADRLKVPPSSWGDLLKPAPALVGHIAMPDDQEE
CCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCHHHHEECCCCCCHHH
LLAAALNYLGRPMNSGDPQDLRRAFDLLRAQAPAVLIYGLPVTAQQERSVHDLIDIGLGG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
TGDEKTLNMTTDQTQPWRFVTPREGVMVWVDCLAVLSRTSRPDLAEKFIRFLVRPDNAVR
CCCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
DAIFLHQPTPNPRAEAMLPRAVRDDPQIYPPQSYPIIYRKPVPTDSLALRRRILGALIAI
CEEEEECCCCCCHHHHHCCHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHE
HDAQ
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]