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The map label for this gene is proP [H]

Identifier: 162148308

GI number: 162148308

Start: 2618531

End: 2619856

Strand: Direct

Name: proP [H]

Synonym: GDI_2525

Alternate gene names: 162148308

Gene position: 2618531-2619856 (Clockwise)

Preceding gene: 162148306

Following gene: 162148309

Centisome position: 66.39

GC content: 69.76

Gene sequence:

>1326_bases
ATGCCTGAATTCCCTCCCCCGCAAACGGGCAGCCTGACCTGGGGGGAATTGCGCACCCTGGCCCTCGCCGCGCTGGGCGG
GGCGCTGGAATTCTATGATTTCGTGATCTTCGCCTATTTCGCGAAAATCATCGCCCAGCATTTCTTCCCCGCGATCCAGC
CCGACTGGCTGCGCCAGACCGAGACGTTCGGCCTGTTCGGGGCAGGATACCTGGCCCGGCCGCTGGGCGGGGTGGTCATG
GCGCATTTCGGCGACAGGCACGGGCGCAAGAAAATGTTCGCCTTCAGCGTCCTGCTGATGGCGGCCCCCACCCTGCTGAT
GGGGCTTCTGCCCGGTTTCGGCACGCTGGGCATCGGCGCCCCGCTGGCCCTGCTGCTGCTGCGCATGGCACAGGGGGCCG
CGATCGGCGGCGAGGCGCCGGGCGGCTGGGTCTTCGTGGCCGAACATGCCAGCGCCGGACGCACCGGACTGGCCTGCGGG
CTGCTGACCGGCGGACTGACGGCCGGGATGCTGATCGGCTCGCTGGTGGCGCTGGGCCTGAACCTGGCCCTGCCGCCCGC
CGCGATCGTCGCATGGGGATGGCGCCTGCCCTTCATCCTGGGCGGCATCTTCGGCCTGATCGCCATGAAACTGCGCCGCT
GGCTGGAGGAAACGCCGGTCTTTACCGAGATGCACGCGCGGGATGCGCTGGCGCGGCAGACGCCGCTGGTCGCGGTGCTG
CGCACCTGCCGCCGGGCGGTCATCCTGTCCATCATCGGCACCTGGACGCTGACGGCCGCGATCGTCGTGCTGCTGCTGAT
GATGCCGTCGCTGCTGCAGACGCTGTACGGTTTTTCCCCCGCCCGCACCATGGATGCCAGCCTGGTCGCGACCTTGTCCG
TCACCGTCGCTTCGGTCGTGATCGGCGCGGCCACCGACCGGTGGGGCCTGCGCGGAATGATCCCCCCGGTGGTCGGGCTG
CTGATCGCGGGGGCATGGGGGCTGTATGCGCTGGCCCCCGCCCATCCCGGCTGGTTCCTGCCGCTGGCCGCGCTGGCGGG
TATCGGCGCGGGATACGTCAGCCTCGTGCCGATCGCGATGGTGCGGGCCTTTCCCGCTGCCGTGCGGTTTTCCGGGCTGT
CCTTTTCCTACAACCTGTCATACGCCGTCTTCGGCGGGCTGACCCCGGCCTGCGTGTCGGCCCTGGTGGGGCATGACCCG
CAGGCGCCCGCGCTGTATGTGATCGGGACGGCGCTGGCAGGGGGTGCCGCGCTGTGGGCCATGCCCGGCGCGAAACGGGA
CCTGACGCGGACGTGCCATGGACGGGAATCCCCCTTCCATGCATAA

Upstream 100 bases:

>100_bases
CAGGATTCCGGACAGCCCTGCCCTTTCTCCTTGACAAGGCAGGCGCAAGGCGGGCCGTTCTGCCCGCCCGACCCTTTCCC
AAGCCCGGATCGCGCCCGCC

Downstream 100 bases:

>100_bases
AAATACCATGAGCAGGACATCGCCCGACATCATCGCCCGCCGCATCCGCCAGGGAAGTGGACAGGAACCGCCTGACCTGG
TGGTCCGCGACGTCCGCCTG

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPEFPPPQTGSLTWGELRTLALAALGGALEFYDFVIFAYFAKIIAQHFFPAIQPDWLRQTETFGLFGAGYLARPLGGVVM
AHFGDRHGRKKMFAFSVLLMAAPTLLMGLLPGFGTLGIGAPLALLLLRMAQGAAIGGEAPGGWVFVAEHASAGRTGLACG
LLTGGLTAGMLIGSLVALGLNLALPPAAIVAWGWRLPFILGGIFGLIAMKLRRWLEETPVFTEMHARDALARQTPLVAVL
RTCRRAVILSIIGTWTLTAAIVVLLLMMPSLLQTLYGFSPARTMDASLVATLSVTVASVVIGAATDRWGLRGMIPPVVGL
LIAGAWGLYALAPAHPGWFLPLAALAGIGAGYVSLVPIAMVRAFPAAVRFSGLSFSYNLSYAVFGGLTPACVSALVGHDP
QAPALYVIGTALAGGAALWAMPGAKRDLTRTCHGRESPFHA

Sequences:

>Translated_441_residues
MPEFPPPQTGSLTWGELRTLALAALGGALEFYDFVIFAYFAKIIAQHFFPAIQPDWLRQTETFGLFGAGYLARPLGGVVM
AHFGDRHGRKKMFAFSVLLMAAPTLLMGLLPGFGTLGIGAPLALLLLRMAQGAAIGGEAPGGWVFVAEHASAGRTGLACG
LLTGGLTAGMLIGSLVALGLNLALPPAAIVAWGWRLPFILGGIFGLIAMKLRRWLEETPVFTEMHARDALARQTPLVAVL
RTCRRAVILSIIGTWTLTAAIVVLLLMMPSLLQTLYGFSPARTMDASLVATLSVTVASVVIGAATDRWGLRGMIPPVVGL
LIAGAWGLYALAPAHPGWFLPLAALAGIGAGYVSLVPIAMVRAFPAAVRFSGLSFSYNLSYAVFGGLTPACVSALVGHDP
QAPALYVIGTALAGGAALWAMPGAKRDLTRTCHGRESPFHA
>Mature_440_residues
PEFPPPQTGSLTWGELRTLALAALGGALEFYDFVIFAYFAKIIAQHFFPAIQPDWLRQTETFGLFGAGYLARPLGGVVMA
HFGDRHGRKKMFAFSVLLMAAPTLLMGLLPGFGTLGIGAPLALLLLRMAQGAAIGGEAPGGWVFVAEHASAGRTGLACGL
LTGGLTAGMLIGSLVALGLNLALPPAAIVAWGWRLPFILGGIFGLIAMKLRRWLEETPVFTEMHARDALARQTPLVAVLR
TCRRAVILSIIGTWTLTAAIVVLLLMMPSLLQTLYGFSPARTMDASLVATLSVTVASVVIGAATDRWGLRGMIPPVVGLL
IAGAWGLYALAPAHPGWFLPLAALAGIGAGYVSLVPIAMVRAFPAAVRFSGLSFSYNLSYAVFGGLTPACVSALVGHDPQ
APALYVIGTALAGGAALWAMPGAKRDLTRTCHGRESPFHA

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=31.2056737588652, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=29.185520361991, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788942, Length=374, Percent_Identity=32.3529411764706, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789941, Length=386, Percent_Identity=30.5699481865285, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87082231, Length=333, Percent_Identity=25.8258258258258, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46221; Mature: 46090

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFPPPQTGSLTWGELRTLALAALGGALEFYDFVIFAYFAKIIAQHFFPAIQPDWLRQT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ETFGLFGAGYLARPLGGVVMAHFGDRHGRKKMFAFSVLLMAAPTLLMGLLPGFGTLGIGA
HHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
PLALLLLRMAQGAAIGGEAPGGWVFVAEHASAGRTGLACGLLTGGLTAGMLIGSLVALGL
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLALPPAAIVAWGWRLPFILGGIFGLIAMKLRRWLEETPVFTEMHARDALARQTPLVAVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
RTCRRAVILSIIGTWTLTAAIVVLLLMMPSLLQTLYGFSPARTMDASLVATLSVTVASVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IGAATDRWGLRGMIPPVVGLLIAGAWGLYALAPAHPGWFLPLAALAGIGAGYVSLVPIAM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VRAFPAAVRFSGLSFSYNLSYAVFGGLTPACVSALVGHDPQAPALYVIGTALAGGAALWA
HHHHHHHHHCCCCEEEECCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEHHHHHCCHHEEC
MPGAKRDLTRTCHGRESPFHA
CCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PEFPPPQTGSLTWGELRTLALAALGGALEFYDFVIFAYFAKIIAQHFFPAIQPDWLRQT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ETFGLFGAGYLARPLGGVVMAHFGDRHGRKKMFAFSVLLMAAPTLLMGLLPGFGTLGIGA
HHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
PLALLLLRMAQGAAIGGEAPGGWVFVAEHASAGRTGLACGLLTGGLTAGMLIGSLVALGL
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NLALPPAAIVAWGWRLPFILGGIFGLIAMKLRRWLEETPVFTEMHARDALARQTPLVAVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHH
RTCRRAVILSIIGTWTLTAAIVVLLLMMPSLLQTLYGFSPARTMDASLVATLSVTVASVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IGAATDRWGLRGMIPPVVGLLIAGAWGLYALAPAHPGWFLPLAALAGIGAGYVSLVPIAM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHH
VRAFPAAVRFSGLSFSYNLSYAVFGGLTPACVSALVGHDPQAPALYVIGTALAGGAALWA
HHHHHHHHHCCCCEEEECCCHHHHCCCCHHHHHHHHCCCCCCCEEEEEHHHHHCCHHEEC
MPGAKRDLTRTCHGRESPFHA
CCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA