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The map label for this gene is nuoM [H]

Identifier: 162148243

GI number: 162148243

Start: 2549588

End: 2551069

Strand: Reverse

Name: nuoM [H]

Synonym: GDI_2460

Alternate gene names: 162148243

Gene position: 2551069-2549588 (Counterclockwise)

Preceding gene: 162148244

Following gene: 162148242

Centisome position: 64.68

GC content: 71.19

Gene sequence:

>1482_bases
ATGATCGCGTTGCCGGCCCTGGTCCTGCTGCCGCTGGCCGGCGGGACGGCGGCGTGGCTGGCCGGACGCGGCGCCGGCCC
GCGCGGGCCGTGGCGCGTGACGCTGGCCACGCTGGCGCTGCTGACCCTGCTGCTGCTGTACATCACCTGGCGTGCGTACT
GGCACGCCCCGGCGGGCGGCGGGCCGTGGCTGGCGCATTTCGCCGTGCCGTGGATTCCCGCCCTGGGCATCGCGGTGCGC
TGCGACCTGGACGGGCTGGGGCTGGTTATGCTGTGGCTGACGGTGGTGCTGTCCTGGCCGTGCGTGCTGCTGGCCTCGCG
CGATATCGGGCCGGGGGCCGGGGTGTTTCACCTGCTGCTGCTGGCGACGGTCGCGGGGGTGGACGGCGTGTTCATCGCGA
CCGACCTGTTTCTGTTCTTCTTCTTCTGGGAACTGATGCTGGTACCGCTCTATGTCCTGATCACCGGCTGGGGGCACGAG
GACCGGGCGCGCGCGGCCATCAAGTTCTTCCTGTTCACCCAGGGCAGCGGCCTGCTGATGCTGCTGTCCATCCTGGGTCT
GGCCATCGCGCATTTTCGCCAGACCGGGGTCCTGACCTTCGATTATTTCGCCCTGGCGGGCACGATGCTGGCGCCGGGCG
TGGCGCTGGCGCTGGCGTCGGGGTTCTTCGTCGCCTTCGCGGTGAAGCTGCCGGTGGTGCCGCTGCATGTCTGGCTGCCC
GACACCCACACCCAGGCCCCCACGGCGGGCAGCGTGCTGCTGGCCGGGGTGCTGCTGAAGACCGGCGGTTACGGGCTGAT
CCGCTTCGTCGCGCTGCTGTTTCCCGACGCCGCGTCACGGCTGGCGCCGCTGGCGCTGGTGCTGGGGGTGGCGGGCGTCC
TGTACGGCGCGATGCAGGCACTGGTGCAGGACGACCTGAAACGGCTGATCGCCTATAGCAGCGTCAGCCATCTGGGGTTC
GTCCTGATGGGGGTTTTCTCGGGCTCGGCCCTGGGGATGGAAGGGGCGGTGGTGCAGATGCTGGCCCATGGGCTGAGCAC
CGGCGCGCTGTTCGTCATCGCCGGGGCGCTGCAGGACCGGCTGCATACCCGCGACCTGCGCCGCATGGGCGGATTGTGGG
CGGTGATGCCGCATCTGTCCGCCATGGGGCTGGTCTTCGCGGTGGCGTCGCTGGGCCTGCCGGGCCTGGGCAATTTCGTG
GGCGAATTCCTGGTGCTGCTGGCGACGTGGCGCATCAGTCCGCTGCTGGCCGTCCTGGCGGCGTCGGGGCTGGTCCTGTC
CGCGCTGTACGGCCTGACCATGGTCAGCCGGGCCTTCCAGGGTCCCCTTCCGGTGGACGGGGTCCGGGGGGCCGGCGATT
TCGGGGCGCGGCACATGGCGGTACTGGGCGGCACGGTCGCGCTGCTGATCGGCCTGGGCCTGTGCCCGCAGCCGGTGCTG
GACCTGTCGCAGCCCGCCCGCCCGACGGTGTCCGCGCCATGA

Upstream 100 bases:

>100_bases
GACTGATGCAGGACGGCCAGGTCCGGCGCTATGCCGGGTGGCTGGCCGTGGGTACGGCGGTCGCCCTGTGCCTGGCGGTG
CAGCCATGGGGGGGACGGCC

Downstream 100 bases:

>100_bases
CTCATGACGCCATGACCATATTGCCCGCCATGCCGCCGCCCGTGCTGGTCCTGGGGGCCGGCATCCTGGCCCTGCTGGCC
TGCCTGGCATGGCGGCGCGC

Product: NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MIALPALVLLPLAGGTAAWLAGRGAGPRGPWRVTLATLALLTLLLLYITWRAYWHAPAGGGPWLAHFAVPWIPALGIAVR
CDLDGLGLVMLWLTVVLSWPCVLLASRDIGPGAGVFHLLLLATVAGVDGVFIATDLFLFFFFWELMLVPLYVLITGWGHE
DRARAAIKFFLFTQGSGLLMLLSILGLAIAHFRQTGVLTFDYFALAGTMLAPGVALALASGFFVAFAVKLPVVPLHVWLP
DTHTQAPTAGSVLLAGVLLKTGGYGLIRFVALLFPDAASRLAPLALVLGVAGVLYGAMQALVQDDLKRLIAYSSVSHLGF
VLMGVFSGSALGMEGAVVQMLAHGLSTGALFVIAGALQDRLHTRDLRRMGGLWAVMPHLSAMGLVFAVASLGLPGLGNFV
GEFLVLLATWRISPLLAVLAASGLVLSALYGLTMVSRAFQGPLPVDGVRGAGDFGARHMAVLGGTVALLIGLGLCPQPVL
DLSQPARPTVSAP

Sequences:

>Translated_493_residues
MIALPALVLLPLAGGTAAWLAGRGAGPRGPWRVTLATLALLTLLLLYITWRAYWHAPAGGGPWLAHFAVPWIPALGIAVR
CDLDGLGLVMLWLTVVLSWPCVLLASRDIGPGAGVFHLLLLATVAGVDGVFIATDLFLFFFFWELMLVPLYVLITGWGHE
DRARAAIKFFLFTQGSGLLMLLSILGLAIAHFRQTGVLTFDYFALAGTMLAPGVALALASGFFVAFAVKLPVVPLHVWLP
DTHTQAPTAGSVLLAGVLLKTGGYGLIRFVALLFPDAASRLAPLALVLGVAGVLYGAMQALVQDDLKRLIAYSSVSHLGF
VLMGVFSGSALGMEGAVVQMLAHGLSTGALFVIAGALQDRLHTRDLRRMGGLWAVMPHLSAMGLVFAVASLGLPGLGNFV
GEFLVLLATWRISPLLAVLAASGLVLSALYGLTMVSRAFQGPLPVDGVRGAGDFGARHMAVLGGTVALLIGLGLCPQPVL
DLSQPARPTVSAP
>Mature_493_residues
MIALPALVLLPLAGGTAAWLAGRGAGPRGPWRVTLATLALLTLLLLYITWRAYWHAPAGGGPWLAHFAVPWIPALGIAVR
CDLDGLGLVMLWLTVVLSWPCVLLASRDIGPGAGVFHLLLLATVAGVDGVFIATDLFLFFFFWELMLVPLYVLITGWGHE
DRARAAIKFFLFTQGSGLLMLLSILGLAIAHFRQTGVLTFDYFALAGTMLAPGVALALASGFFVAFAVKLPVVPLHVWLP
DTHTQAPTAGSVLLAGVLLKTGGYGLIRFVALLFPDAASRLAPLALVLGVAGVLYGAMQALVQDDLKRLIAYSSVSHLGF
VLMGVFSGSALGMEGAVVQMLAHGLSTGALFVIAGALQDRLHTRDLRRMGGLWAVMPHLSAMGLVFAVASLGLPGLGNFV
GEFLVLLATWRISPLLAVLAASGLVLSALYGLTMVSRAFQGPLPVDGVRGAGDFGARHMAVLGGTVALLIGLGLCPQPVL
DLSQPARPTVSAP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=315, Percent_Identity=36.1904761904762, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI251831117, Length=345, Percent_Identity=25.7971014492754, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=492, Percent_Identity=48.780487804878, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788827, Length=322, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1788831, Length=186, Percent_Identity=34.9462365591398, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788829, Length=298, Percent_Identity=29.1946308724832, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI145693160, Length=302, Percent_Identity=28.476821192053, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=234, Percent_Identity=30.3418803418803, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 51618; Mature: 51618

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPALVLLPLAGGTAAWLAGRGAGPRGPWRVTLATLALLTLLLLYITWRAYWHAPAGG
CCCCHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GPWLAHFAVPWIPALGIAVRCDLDGLGLVMLWLTVVLSWPCVLLASRDIGPGAGVFHLLL
CCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LATVAGVDGVFIATDLFLFFFFWELMLVPLYVLITGWGHEDRARAAIKFFLFTQGSGLLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCHHH
LLSILGLAIAHFRQTGVLTFDYFALAGTMLAPGVALALASGFFVAFAVKLPVVPLHVWLP
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DTHTQAPTAGSVLLAGVLLKTGGYGLIRFVALLFPDAASRLAPLALVLGVAGVLYGAMQA
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVQDDLKRLIAYSSVSHLGFVLMGVFSGSALGMEGAVVQMLAHGLSTGALFVIAGALQDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
LHTRDLRRMGGLWAVMPHLSAMGLVFAVASLGLPGLGNFVGEFLVLLATWRISPLLAVLA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASGLVLSALYGLTMVSRAFQGPLPVDGVRGAGDFGARHMAVLGGTVALLIGLGLCPQPVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
DLSQPARPTVSAP
CCCCCCCCCCCCC
>Mature Secondary Structure
MIALPALVLLPLAGGTAAWLAGRGAGPRGPWRVTLATLALLTLLLLYITWRAYWHAPAGG
CCCCHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GPWLAHFAVPWIPALGIAVRCDLDGLGLVMLWLTVVLSWPCVLLASRDIGPGAGVFHLLL
CCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LATVAGVDGVFIATDLFLFFFFWELMLVPLYVLITGWGHEDRARAAIKFFLFTQGSGLLM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEEEEECCCCHHH
LLSILGLAIAHFRQTGVLTFDYFALAGTMLAPGVALALASGFFVAFAVKLPVVPLHVWLP
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
DTHTQAPTAGSVLLAGVLLKTGGYGLIRFVALLFPDAASRLAPLALVLGVAGVLYGAMQA
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVQDDLKRLIAYSSVSHLGFVLMGVFSGSALGMEGAVVQMLAHGLSTGALFVIAGALQDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
LHTRDLRRMGGLWAVMPHLSAMGLVFAVASLGLPGLGNFVGEFLVLLATWRISPLLAVLA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASGLVLSALYGLTMVSRAFQGPLPVDGVRGAGDFGARHMAVLGGTVALLIGLGLCPQPVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHH
DLSQPARPTVSAP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]