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The map label for this gene is rhtB [H]

Identifier: 162148212

GI number: 162148212

Start: 2515580

End: 2516203

Strand: Direct

Name: rhtB [H]

Synonym: GDI_2429

Alternate gene names: 162148212

Gene position: 2515580-2516203 (Clockwise)

Preceding gene: 162148211

Following gene: 162148214

Centisome position: 63.78

GC content: 67.63

Gene sequence:

>624_bases
ATGACGCTGCAATCCTGGCTGCTCTACCTGAACGCAGTGTTCCTGCTGTCGGCCATTCCCGGGCCGAACATGACGCACAT
CATGACCGTCAGCGTCCGAAACGGCATCTGGCGGACCGTGCCGGGCATGGTCGGATGCCTGCTGGCGCTGGTCGCGCTGA
TGGGCGCATCGGCATTGGGACTCAGCGCCGTGCTGACGGCGTCGCCCACCCTGTTCAGGGTCCTGAAATTCGCCGGCGCG
TTCTATCTGGTCCAGATGGGCGTGCGGGCCTGGCTGGATCGCGGGTCGGCCCCGGCGGGGCCGGACGCGGCCGTTCCCCT
GGCGTGGCATTCCACGCTGGGCACGTCCTTCCTGGTCGGGGCCAGCAACCCCAAGGCCATCATCTTCGCGGCATCGTTCC
TGCCGCAATTCATCAACCCCACGGCGCCGCAGGCCCCGCAATACGGGCTGCTGGTCGCGACCTCGGCCGTGGTCGAGACG
CTGTGGTATCTGATCTATGCCAGCAGCGGCGCACGCCTGGCCGTCGTGCTGACCCGCGCCGTGTGGCGGCGGCGCGTGCA
GCGGTTCAGTGGCGCGCTGTTCATCCTGTTCGGCTTCGCGCTGCTGCTGCACGATCCGAAATAG

Upstream 100 bases:

>100_bases
TCATCGGTGACAGCGCCCGCTTCATGTTCGCCTGGGATTCGGAGCGTGCGCGCATCGACGCGCTGGCGGCGGACCTGCGC
GCCATCACCCGCACGCCGGC

Downstream 100 bases:

>100_bases
AACGGGCCCGTCATAGATCCGGGATCGTCACCCGCCCTTCGATACGAGTCACCGTTTCGCCGCCGATCCAGATATCGGAC
CCGATCCGGGTCACCGTAAC

Product: lysine exporter protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTLQSWLLYLNAVFLLSAIPGPNMTHIMTVSVRNGIWRTVPGMVGCLLALVALMGASALGLSAVLTASPTLFRVLKFAGA
FYLVQMGVRAWLDRGSAPAGPDAAVPLAWHSTLGTSFLVGASNPKAIIFAASFLPQFINPTAPQAPQYGLLVATSAVVET
LWYLIYASSGARLAVVLTRAVWRRRVQRFSGALFILFGFALLLHDPK

Sequences:

>Translated_207_residues
MTLQSWLLYLNAVFLLSAIPGPNMTHIMTVSVRNGIWRTVPGMVGCLLALVALMGASALGLSAVLTASPTLFRVLKFAGA
FYLVQMGVRAWLDRGSAPAGPDAAVPLAWHSTLGTSFLVGASNPKAIIFAASFLPQFINPTAPQAPQYGLLVATSAVVET
LWYLIYASSGARLAVVLTRAVWRRRVQRFSGALFILFGFALLLHDPK
>Mature_206_residues
TLQSWLLYLNAVFLLSAIPGPNMTHIMTVSVRNGIWRTVPGMVGCLLALVALMGASALGLSAVLTASPTLFRVLKFAGAF
YLVQMGVRAWLDRGSAPAGPDAAVPLAWHSTLGTSFLVGASNPKAIIFAASFLPQFINPTAPQAPQYGLLVATSAVVETL
WYLIYASSGARLAVVLTRAVWRRRVQRFSGALFILFGFALLLHDPK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788099, Length=173, Percent_Identity=27.7456647398844, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22173; Mature: 22041

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQSWLLYLNAVFLLSAIPGPNMTHIMTVSVRNGIWRTVPGMVGCLLALVALMGASALG
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHH
LSAVLTASPTLFRVLKFAGAFYLVQMGVRAWLDRGSAPAGPDAAVPLAWHSTLGTSFLVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEE
ASNPKAIIFAASFLPQFINPTAPQAPQYGLLVATSAVVETLWYLIYASSGARLAVVLTRA
CCCCCEEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VWRRRVQRFSGALFILFGFALLLHDPK
HHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLQSWLLYLNAVFLLSAIPGPNMTHIMTVSVRNGIWRTVPGMVGCLLALVALMGASALG
CHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHH
LSAVLTASPTLFRVLKFAGAFYLVQMGVRAWLDRGSAPAGPDAAVPLAWHSTLGTSFLVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEEEEE
ASNPKAIIFAASFLPQFINPTAPQAPQYGLLVATSAVVETLWYLIYASSGARLAVVLTRA
CCCCCEEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VWRRRVQRFSGALFILFGFALLLHDPK
HHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]