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The map label for this gene is rsmB [H]

Identifier: 162148166

GI number: 162148166

Start: 2457176

End: 2458477

Strand: Reverse

Name: rsmB [H]

Synonym: GDI_2383

Alternate gene names: 162148166

Gene position: 2458477-2457176 (Counterclockwise)

Preceding gene: 162148173

Following gene: 162148165

Centisome position: 62.33

GC content: 75.73

Gene sequence:

>1302_bases
ATGATTCAGGGCGTACCAGACAGGTCAGGCGCGCGGGGGCGCGGCCCGCGCGGGGCCGCGGCGGACCCGACGCGGGATAT
CGCCTTCGATATCGTGCAGGGGGTGCTGGAACACCGGCGCATGCTGGAAACCAGCCTGGACCAGTCCGCCGCCGCACGGG
ACGCGGACGCGCGCGACCGTGCCGCCGCGCACCGTCTGGCGGCGACCGTGCTGCGTCACATGGGCACGGCGGGCATGGTG
CTGGAACCCTTCCTGAAGAAGGAGCCGCCCCTGCCGGTGCGCACGGTGCTGCTGATCGGCGTGGCGCAGATCCTGTTCCT
GGACACGCCCGCCCATGCGGCGGTCGGCACGGCGGTCTCGCTGGCGCGCCGGCGGGGGCTGGTGCCGTTCGCGGGACTGG
TCAACGCCGTGCTGCGCCGGGTGGCCCAGGCCGGCGCCGGGGCGCTGGAGGGGCTGGACCAGGCCCGGCTGGACGTGCCC
CCCTGGCTGTGGGGCGCATGGGGCAGCCTGGCGCGCCCGGTGGCCGAGGCGCTGGGCCAGGAGGCCCCGCTGGACCTGAC
ATTGCGGCCGGGGCTGCCGGAGGCCGAACGGCCGCCCGGCGGGGTGATGCTGCCCACCGGGACCCTGCGCCTGCCGGCCG
GCACCCGTGTGGCCGACCTGCCGGGCTATGCGCAGGGCGCGTTCTGGGTGCAGGACGCGGCCGCCGCCCTGCCGGCGCGC
CTGCTGGCGCCCCGCGCGGGTGAGCGGATCGCGGACCTGTGCGCCGCCCCGGGGGGCAAGACGGCACAACTGGCCGTGGC
GGGGGCCGAGGTCACGGCCATCGAGCGGGAGCCGGGCCGGCTGGCGCGGCTGAAGGAGAACTGGACCGCCTCGGACTGGC
CGACCGGGTGCAAACTGGTCCAGGGCGACGCCGCCACGTGGCGGCCGGACGCGCCGCTGGACGCCGTGCTGCTGGATGCG
CCCTGTTCGGCGACGGGCACCGCACACCGCCACCCCGACGTGCTGTGGATCAAGCGCCCGCGCCTGACGGCGCTGACCGA
GGGCCAGGACCGGCGGCGCGCGACATGCTGCGTCCCCTGTCCGGCTGGTCTATGCGGTGTGCTCGCTCCAGCCCGACGTA
GGGCCGGAACGCGTACGCGCGGCCGAGGCGATGGGCTGGAACCCGATCCCTTCACGGCGGAGGAACTGGCCGTGCTGCCC
GAAGCCCTGACGAAGGAAGGCTGGCTGCGCACCCATCCGGGCCTGTGGACGGAACAGGGCGGCATGGACGGATTCTTCGC
GGCGCGGTTCCGCCGCCGGTAG

Upstream 100 bases:

>100_bases
GCAGAATTCCCCGGCGGGGGACTGGCGGCCCGGGGGCGGGCGTGCGATCAGCACGCCGGATGTCGAACCAACGATCGGGA
ACGGGGCAAGTCGGGCGGAT

Downstream 100 bases:

>100_bases
GCCGGGCCAGGGCAGGCACGGGACCCAGGGCCGGCATGGAACCCAGGGCAGCAGGGGACATGGACGCGGGCAAGCACGAT
CGTGATCCGCCGGGATGCGA

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIQGVPDRSGARGRGPRGAAADPTRDIAFDIVQGVLEHRRMLETSLDQSAAARDADARDRAAAHRLAATVLRHMGTAGMV
LEPFLKKEPPLPVRTVLLIGVAQILFLDTPAHAAVGTAVSLARRRGLVPFAGLVNAVLRRVAQAGAGALEGLDQARLDVP
PWLWGAWGSLARPVAEALGQEAPLDLTLRPGLPEAERPPGGVMLPTGTLRLPAGTRVADLPGYAQGAFWVQDAAAALPAR
LLAPRAGERIADLCAAPGGKTAQLAVAGAEVTAIEREPGRLARLKENWTASDWPTGCKLVQGDAATWRPDAPLDAVLLDA
PCSATGTAHRHPDVLWIKRPRLTALTEGQDRRRATCCVPCPAGLCGVLAPARRRAGTRTRGRGDGLEPDPFTAEELAVLP
EALTKEGWLRTHPGLWTEQGGMDGFFAARFRRR

Sequences:

>Translated_433_residues
MIQGVPDRSGARGRGPRGAAADPTRDIAFDIVQGVLEHRRMLETSLDQSAAARDADARDRAAAHRLAATVLRHMGTAGMV
LEPFLKKEPPLPVRTVLLIGVAQILFLDTPAHAAVGTAVSLARRRGLVPFAGLVNAVLRRVAQAGAGALEGLDQARLDVP
PWLWGAWGSLARPVAEALGQEAPLDLTLRPGLPEAERPPGGVMLPTGTLRLPAGTRVADLPGYAQGAFWVQDAAAALPAR
LLAPRAGERIADLCAAPGGKTAQLAVAGAEVTAIEREPGRLARLKENWTASDWPTGCKLVQGDAATWRPDAPLDAVLLDA
PCSATGTAHRHPDVLWIKRPRLTALTEGQDRRRATCCVPCPAGLCGVLAPARRRAGTRTRGRGDGLEPDPFTAEELAVLP
EALTKEGWLRTHPGLWTEQGGMDGFFAARFRRR
>Mature_433_residues
MIQGVPDRSGARGRGPRGAAADPTRDIAFDIVQGVLEHRRMLETSLDQSAAARDADARDRAAAHRLAATVLRHMGTAGMV
LEPFLKKEPPLPVRTVLLIGVAQILFLDTPAHAAVGTAVSLARRRGLVPFAGLVNAVLRRVAQAGAGALEGLDQARLDVP
PWLWGAWGSLARPVAEALGQEAPLDLTLRPGLPEAERPPGGVMLPTGTLRLPAGTRVADLPGYAQGAFWVQDAAAALPAR
LLAPRAGERIADLCAAPGGKTAQLAVAGAEVTAIEREPGRLARLKENWTASDWPTGCKLVQGDAATWRPDAPLDAVLLDA
PCSATGTAHRHPDVLWIKRPRLTALTEGQDRRRATCCVPCPAGLCGVLAPARRRAGTRTRGRGDGLEPDPFTAEELAVLP
EALTKEGWLRTHPGLWTEQGGMDGFFAARFRRR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Escherichia coli, GI2367212, Length=349, Percent_Identity=30.945558739255, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17536757, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 45940; Mature: 45940

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGVPDRSGARGRGPRGAAADPTRDIAFDIVQGVLEHRRMLETSLDQSAAARDADARDR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AAAHRLAATVLRHMGTAGMVLEPFLKKEPPLPVRTVLLIGVAQILFLDTPAHAAVGTAVS
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LARRRGLVPFAGLVNAVLRRVAQAGAGALEGLDQARLDVPPWLWGAWGSLARPVAEALGQ
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
EAPLDLTLRPGLPEAERPPGGVMLPTGTLRLPAGTRVADLPGYAQGAFWVQDAAAALPAR
CCCCEEEECCCCCCCCCCCCCEECCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LLAPRAGERIADLCAAPGGKTAQLAVAGAEVTAIEREPGRLARLKENWTASDWPTGCKLV
HHCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEE
QGDAATWRPDAPLDAVLLDAPCSATGTAHRHPDVLWIKRPRLTALTEGQDRRRATCCVPC
ECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEECC
PAGLCGVLAPARRRAGTRTRGRGDGLEPDPFTAEELAVLPEALTKEGWLRTHPGLWTEQG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCC
GMDGFFAARFRRR
CCCHHHHHHHHCC
>Mature Secondary Structure
MIQGVPDRSGARGRGPRGAAADPTRDIAFDIVQGVLEHRRMLETSLDQSAAARDADARDR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AAAHRLAATVLRHMGTAGMVLEPFLKKEPPLPVRTVLLIGVAQILFLDTPAHAAVGTAVS
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LARRRGLVPFAGLVNAVLRRVAQAGAGALEGLDQARLDVPPWLWGAWGSLARPVAEALGQ
HHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
EAPLDLTLRPGLPEAERPPGGVMLPTGTLRLPAGTRVADLPGYAQGAFWVQDAAAALPAR
CCCCEEEECCCCCCCCCCCCCEECCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LLAPRAGERIADLCAAPGGKTAQLAVAGAEVTAIEREPGRLARLKENWTASDWPTGCKLV
HHCCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEE
QGDAATWRPDAPLDAVLLDAPCSATGTAHRHPDVLWIKRPRLTALTEGQDRRRATCCVPC
ECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEEECC
PAGLCGVLAPARRRAGTRTRGRGDGLEPDPFTAEELAVLPEALTKEGWLRTHPGLWTEQG
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCC
GMDGFFAARFRRR
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA