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The map label for this gene is dapF [H]

Identifier: 162148089

GI number: 162148089

Start: 2369511

End: 2370347

Strand: Reverse

Name: dapF [H]

Synonym: GDI_2306

Alternate gene names: 162148089

Gene position: 2370347-2369511 (Counterclockwise)

Preceding gene: 162148090

Following gene: 162148088

Centisome position: 60.1

GC content: 72.16

Gene sequence:

>837_bases
ATGACCCCGTTCCACAAGATGCACGGCCTGGGCAACGATTTCGTCGTGGTGGACGAACGCGCCGAACGGTACGGCCTGAC
GCCGGCCCGCATCGCGGCGCTGGCCGACCGCCATCGCGGGATCGGCTGCGACCAGTTCGTGGTCCTGCAACCCGCCACCC
GCGACGGCGCGGACGTCCTGGTGCGGTTCTTCAACGCCGACGGCACCGAGGCCGGGGCCTGTGGCAATGCCTCGCGCTGC
GTCGCGGCGCTGCTGTGGCGGCAGGAGGGGCGGCGCGAGACGGTGCTGCAGACCCGGGCCGGCCTGCTGCCTGCCCGGGT
GGATGACGCGGGCGTCGTCACCGTGGACATGGGCCGCCCGGCCCTGGACTGGCGCGACGTGCCGCTGGCGCGGGCGGCCG
ATACGCTGCACCTGCCGCTGGCGGGAGACCCGGCGGCGGCCTCGATGGGCAATCCGCACGCCACGTTCTTCCTGGACGAC
ATGCTGGACCTGGACCCGGTCGTGGCCGGGCCGTTGCTGGAACATGATCCGCTGTTTCCCGAACGGGCCAATATCGGCTT
CGCGCATGTCCGCTCGCGCACCGAACTGCGCCTGCGCGTGTGGGAGCGCGGCAGCGGCCTGACCCTGGCCTGCGGCTCCG
GCGCCTGTGCGGCGGTGGTGAACGCCGTCCGGCGCGGGCTGGTGGAACGGACCTGCACGGTCGGGATGGATGGCGGCGCG
CTGTCCGTTTCGTGGCGCGCGCAGGATGACCCAGGGGAAGGGGGGCATGTGCTGATGACCGGCCCCGCCACCGTCAGCTT
CGTCGGCACGTTCGATCCCGAAGGCTATCCGTCATGA

Upstream 100 bases:

>100_bases
TCGGATCGAAGTGCTGCAACGCGGCGCCCCGGGCCGGTTTCTCTGACGGCTGCAAGCTTGTCTCGCACTCCGCCGATGCG
TGCCGTATAAGGCGCGCATG

Downstream 100 bases:

>100_bases
CCGCCAAGCCGGAGATCCTGACCTTCGGGTGCCGCCTGAACACCTATGAGAGCGAGGTGATGCGCGGTCACGCGGCGGCG
CTGGACGACGTGATCATCGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTPFHKMHGLGNDFVVVDERAERYGLTPARIAALADRHRGIGCDQFVVLQPATRDGADVLVRFFNADGTEAGACGNASRC
VAALLWRQEGRRETVLQTRAGLLPARVDDAGVVTVDMGRPALDWRDVPLARAADTLHLPLAGDPAAASMGNPHATFFLDD
MLDLDPVVAGPLLEHDPLFPERANIGFAHVRSRTELRLRVWERGSGLTLACGSGACAAVVNAVRRGLVERTCTVGMDGGA
LSVSWRAQDDPGEGGHVLMTGPATVSFVGTFDPEGYPS

Sequences:

>Translated_278_residues
MTPFHKMHGLGNDFVVVDERAERYGLTPARIAALADRHRGIGCDQFVVLQPATRDGADVLVRFFNADGTEAGACGNASRC
VAALLWRQEGRRETVLQTRAGLLPARVDDAGVVTVDMGRPALDWRDVPLARAADTLHLPLAGDPAAASMGNPHATFFLDD
MLDLDPVVAGPLLEHDPLFPERANIGFAHVRSRTELRLRVWERGSGLTLACGSGACAAVVNAVRRGLVERTCTVGMDGGA
LSVSWRAQDDPGEGGHVLMTGPATVSFVGTFDPEGYPS
>Mature_277_residues
TPFHKMHGLGNDFVVVDERAERYGLTPARIAALADRHRGIGCDQFVVLQPATRDGADVLVRFFNADGTEAGACGNASRCV
AALLWRQEGRRETVLQTRAGLLPARVDDAGVVTVDMGRPALDWRDVPLARAADTLHLPLAGDPAAASMGNPHATFFLDDM
LDLDPVVAGPLLEHDPLFPERANIGFAHVRSRTELRLRVWERGSGLTLACGSGACAAVVNAVRRGLVERTCTVGMDGGAL
SVSWRAQDDPGEGGHVLMTGPATVSFVGTFDPEGYPS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=273, Percent_Identity=41.025641025641, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29618; Mature: 29487

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFHKMHGLGNDFVVVDERAERYGLTPARIAALADRHRGIGCDQFVVLQPATRDGADVL
CCCCHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHE
VRFFNADGTEAGACGNASRCVAALLWRQEGRRETVLQTRAGLLPARVDDAGVVTVDMGRP
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCEEEEECCCC
ALDWRDVPLARAADTLHLPLAGDPAAASMGNPHATFFLDDMLDLDPVVAGPLLEHDPLFP
CCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCC
ERANIGFAHVRSRTELRLRVWERGSGLTLACGSGACAAVVNAVRRGLVERTCTVGMDGGA
CCCCCCHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCE
LSVSWRAQDDPGEGGHVLMTGPATVSFVGTFDPEGYPS
EEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
TPFHKMHGLGNDFVVVDERAERYGLTPARIAALADRHRGIGCDQFVVLQPATRDGADVL
CCCHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHE
VRFFNADGTEAGACGNASRCVAALLWRQEGRRETVLQTRAGLLPARVDDAGVVTVDMGRP
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCEEEEECCCC
ALDWRDVPLARAADTLHLPLAGDPAAASMGNPHATFFLDDMLDLDPVVAGPLLEHDPLFP
CCCCCCCCHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCCCCC
ERANIGFAHVRSRTELRLRVWERGSGLTLACGSGACAAVVNAVRRGLVERTCTVGMDGGA
CCCCCCHHHHCCCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCE
LSVSWRAQDDPGEGGHVLMTGPATVSFVGTFDPEGYPS
EEEEEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA