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The map label for this gene is ispDF [H]

Identifier: 162148053

GI number: 162148053

Start: 2328009

End: 2329163

Strand: Direct

Name: ispDF [H]

Synonym: GDI_2269

Alternate gene names: 162148053

Gene position: 2328009-2329163 (Clockwise)

Preceding gene: 162148043

Following gene: 162148054

Centisome position: 59.02

GC content: 70.22

Gene sequence:

>1155_bases
ATGCGTGTTGCAGCCATTCTCCTTGCCGCAGGCGTGGGCCGACGTTTCAGCGCCACGACGGCATCCCCTGTCGCCAAGCA
GTATCTGACGCTCGGCGGCCGGCCCATCATCCGCCATGCGGCGGATGCCCTGATCCCGCATGTCGACCTGCTTCAGCCGG
TGGGCGATGCCCAGGCCCTGGCCGATGCGCTGCAGGGGCTGGATGTCCTGCCGCCGGTGGCGGGGGGCGAGACGCGGCAG
GAAAGCGTCCGCGCCGGGCTGGAGGCGCTGGACCGCCTGCCCGAGTCCGCGCGGCCCGACCTGGTGCTGATCCATGACGG
CGCCCGCCCCTATGTCCCCGGCGAGGTGACGCAGGCGGTGATCGCGGCGCTGGCGGCCCATCCCGGCGCCATTCCCGCCG
TGCAGGTGGCCGATACGATCAAGCGCGGCAGGGACGGCGTGATTGCCGGCACCGTGCCGCGGGACGACCTGTTTCGCGCC
CAGACGCCGCAGGGCTTCCGCTTCGCGCTGCTGCGCGACCTGCATCGCGGCGCCGCCGCGACGGACGCGACCGACGACGC
GGCGCTGCTGGAGGCAGCCGGCCACACGGTCGCGCTGGTGCCCGGATCGGAAGACAATATCAAGCTGACCTACAAGGAGG
ATCTCATGCGCCTGGAACGCCTGATCGGTCCGACATTGCTGCCGCGCACCGGCATCGGCTACGACGTCCATGCGTTTGCC
GAGAACCGCGCCCTGATCCTGTGCGGCATCACCGTGCCCCACGATCGCGGCCTGGCCGGGCATTCGGACGCCGATGTCGG
TATCCATGCCCTGTGCGACGCGATCTATGGCGCGCTGGCCGAGGGCGATATCGGGCGCCATTTCCCGCCCAGCCAGAATC
AGTGGAAGGACGCCGACAGCGCGCGCTTCCTGGTCCATGCCGGCGAGCGGATTCGCGCCCGGGGCGGCATGCTGGTGAAT
GCCGATGTCACGCTGATCTGCGAACGGCCGAAAATCGGCCCGCATTCCGAGGCGATGCGCCAGCGCCTGGCCGACCTGCT
GCAGGTCGGGATCGACCGGATTTCCGTGAAGGCCACGACCTCCGAACGGCTGGGCTTCACCGGCCGCGAGGAAGGCATCG
CCTGCACCGCCAGCGCCACGGTCCTGGTTCCCTAG

Upstream 100 bases:

>100_bases
CAGGGGCGGAGCCACAAAACCGGGGGCGGAGCCGCAAAATACGGCATCCCCGCGCGTTCCAGCGCTTGACGGCGGGGAAT
CTCCGCCGCATTGCTCCGCC

Downstream 100 bases:

>100_bases
ATGTTTGGTCCCGGGATTTGGTGGTGCATTATTTTCACATGAGTGGAAGGATGCGCTATGGGCCAGGTTCACCACGGAAG
CGCCACGACGACAGCGGCAG

Product: IspD/ispF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MRVAAILLAAGVGRRFSATTASPVAKQYLTLGGRPIIRHAADALIPHVDLLQPVGDAQALADALQGLDVLPPVAGGETRQ
ESVRAGLEALDRLPESARPDLVLIHDGARPYVPGEVTQAVIAALAAHPGAIPAVQVADTIKRGRDGVIAGTVPRDDLFRA
QTPQGFRFALLRDLHRGAAATDATDDAALLEAAGHTVALVPGSEDNIKLTYKEDLMRLERLIGPTLLPRTGIGYDVHAFA
ENRALILCGITVPHDRGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQNQWKDADSARFLVHAGERIRARGGMLVN
ADVTLICERPKIGPHSEAMRQRLADLLQVGIDRISVKATTSERLGFTGREEGIACTASATVLVP

Sequences:

>Translated_384_residues
MRVAAILLAAGVGRRFSATTASPVAKQYLTLGGRPIIRHAADALIPHVDLLQPVGDAQALADALQGLDVLPPVAGGETRQ
ESVRAGLEALDRLPESARPDLVLIHDGARPYVPGEVTQAVIAALAAHPGAIPAVQVADTIKRGRDGVIAGTVPRDDLFRA
QTPQGFRFALLRDLHRGAAATDATDDAALLEAAGHTVALVPGSEDNIKLTYKEDLMRLERLIGPTLLPRTGIGYDVHAFA
ENRALILCGITVPHDRGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQNQWKDADSARFLVHAGERIRARGGMLVN
ADVTLICERPKIGPHSEAMRQRLADLLQVGIDRISVKATTSERLGFTGREEGIACTASATVLVP
>Mature_384_residues
MRVAAILLAAGVGRRFSATTASPVAKQYLTLGGRPIIRHAADALIPHVDLLQPVGDAQALADALQGLDVLPPVAGGETRQ
ESVRAGLEALDRLPESARPDLVLIHDGARPYVPGEVTQAVIAALAAHPGAIPAVQVADTIKRGRDGVIAGTVPRDDLFRA
QTPQGFRFALLRDLHRGAAATDATDDAALLEAAGHTVALVPGSEDNIKLTYKEDLMRLERLIGPTLLPRTGIGYDVHAFA
ENRALILCGITVPHDRGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQNQWKDADSARFLVHAGERIRARGGMLVN
ADVTLICERPKIGPHSEAMRQRLADLLQVGIDRISVKATTSERLGFTGREEGIACTASATVLVP

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=28.3898305084746, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=49.3506493506493, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1789104, Length=239, Percent_Identity=36.4016736401674, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 40575; Mature: 40575

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAAILLAAGVGRRFSATTASPVAKQYLTLGGRPIIRHAADALIPHVDLLQPVGDAQAL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCHHHHH
ADALQGLDVLPPVAGGETRQESVRAGLEALDRLPESARPDLVLIHDGARPYVPGEVTQAV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHH
IAALAAHPGAIPAVQVADTIKRGRDGVIAGTVPRDDLFRAQTPQGFRFALLRDLHRGAAA
HHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCHHHHHHHHHHCCCCC
TDATDDAALLEAAGHTVALVPGSEDNIKLTYKEDLMRLERLIGPTLLPRTGIGYDVHAFA
CCCCCCHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
ENRALILCGITVPHDRGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQNQWKDADS
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARFLVHAGERIRARGGMLVNADVTLICERPKIGPHSEAMRQRLADLLQVGIDRISVKATT
CEEEEECCCHHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHEEEEECC
SERLGFTGREEGIACTASATVLVP
HHHCCCCCCCCCEEEECCCEEECC
>Mature Secondary Structure
MRVAAILLAAGVGRRFSATTASPVAKQYLTLGGRPIIRHAADALIPHVDLLQPVGDAQAL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHCCCCHHHHH
ADALQGLDVLPPVAGGETRQESVRAGLEALDRLPESARPDLVLIHDGARPYVPGEVTQAV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHH
IAALAAHPGAIPAVQVADTIKRGRDGVIAGTVPRDDLFRAQTPQGFRFALLRDLHRGAAA
HHHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCHHHHHHHHHHCCCCC
TDATDDAALLEAAGHTVALVPGSEDNIKLTYKEDLMRLERLIGPTLLPRTGIGYDVHAFA
CCCCCCHHHHHHCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
ENRALILCGITVPHDRGLAGHSDADVGIHALCDAIYGALAEGDIGRHFPPSQNQWKDADS
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARFLVHAGERIRARGGMLVNADVTLICERPKIGPHSEAMRQRLADLLQVGIDRISVKATT
CEEEEECCCHHHHCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHEEEEECC
SERLGFTGREEGIACTASATVLVP
HHHCCCCCCCCCEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA