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The map label for this gene is dus [H]

Identifier: 162148051

GI number: 162148051

Start: 2326699

End: 2327769

Strand: Reverse

Name: dus [H]

Synonym: GDI_2267

Alternate gene names: 162148051

Gene position: 2327769-2326699 (Counterclockwise)

Preceding gene: 162148052

Following gene: 162148050

Centisome position: 59.02

GC content: 70.4

Gene sequence:

>1071_bases
ATGCCGTCCATGTCGTCCGAAATGTTGCGTCCCGTCGATCTGGGTGGGGGGGTGGTCCTGCGCACCCCGGTCATCCTCGC
CCCGATGTCCGGGGTGACGGACCTGCCGTTCCGCCGGCTGGCGCGGCGGCTGGGCGCGGGCCTGGTCATTTCGGAAATGA
TCGCCTCGTGGGCCATGGTGCGGGAGAACCAGGCCACCCTGCGCATGGCCGAGGTCGCCGATGATGACGGCCCGAACGCG
GTGCAGCTGGCCGGCTGCGAGCCCGAGGCCATGGCCCAGGCGGCGCGGATCGCGGTCGATCGCGGCGCGGACATGGTCGA
CATCAATTTCGGCTGCCCGGTCAAGAAGGTGGCGGTCGGCCAGCAGGCGGGCTCGGCGCTGATGCGGGACGAAGCCCTGG
CCGCGCGGCTGCTGGATGCCGTTGTGCGGGCCGTGGACGTGCCGGTGACGCTGAAGATGCGCATGGGCTGGGACCATGCC
AGCCTGAACGCGCCGGCCCTGGCCCGCATCGCGGAACAGACGGGCATCCGCATGGTCACCGTGCATGGCCGCACGCGCCA
GCAATTCTATAACGGCACCGCCGACTGGTCCTTCGTCCGGCAGGTGAAGGACGCGGTATCGCTGCCGGTGATCGTCAACG
GCGATATCCAGACGGTCGACGACGCGCGGCAGGCCCTGGCGCTGTCGGGGGCGGACGGGGTGATGATCGGGCGCGGCTGC
TATGGCCGGCCGTGGTTCCTGGCCCAGGTCGCGCAGGCGCTGACGACCGGCGACATCGTGCCCGACCCCGACCTGGCGAC
GGAAAAGGCGATCGTGCTGGATCATTACGACATGATGCTGTCGCATTTCGGCGCCCATCCCGGGCTGCGCCTGGCGCGCA
AGCATGTCGCCTGGTATTCCGCCGGCCTGCCGGATTCGGCCGGATTCCGCGCCGCCATCAACCGGGTGGACCAGGCCCAG
GAGGCCATCGGGATGATCTCCGCCTTCTATGACCGGCACATCGCTGCCGGTTCCCACCGCGCGCGGCGCGGCACGGCGGC
GGGCGGTCAGGATGCCGCCCAGGCGGCTTGA

Upstream 100 bases:

>100_bases
GGGTATTCTGCTTAAATCCTGGGCATAGATGCCGGAAATTCGCCCAATCCCGGCGCCCATCCGTGTGCCGGGCGCGGGAT
CTGACAGCCCAACGGGGGTA

Downstream 100 bases:

>100_bases
AACCCATGGCGGCCGGCATGGCCCTCGACATGCCGCAGCCGGATGGCGCCGCGCTGCTGGACGCGATGTCGATGCCGGTG
GTCCTGGTGGGGCCGGGCAA

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MPSMSSEMLRPVDLGGGVVLRTPVILAPMSGVTDLPFRRLARRLGAGLVISEMIASWAMVRENQATLRMAEVADDDGPNA
VQLAGCEPEAMAQAARIAVDRGADMVDINFGCPVKKVAVGQQAGSALMRDEALAARLLDAVVRAVDVPVTLKMRMGWDHA
SLNAPALARIAEQTGIRMVTVHGRTRQQFYNGTADWSFVRQVKDAVSLPVIVNGDIQTVDDARQALALSGADGVMIGRGC
YGRPWFLAQVAQALTTGDIVPDPDLATEKAIVLDHYDMMLSHFGAHPGLRLARKHVAWYSAGLPDSAGFRAAINRVDQAQ
EAIGMISAFYDRHIAAGSHRARRGTAAGGQDAAQAA

Sequences:

>Translated_356_residues
MPSMSSEMLRPVDLGGGVVLRTPVILAPMSGVTDLPFRRLARRLGAGLVISEMIASWAMVRENQATLRMAEVADDDGPNA
VQLAGCEPEAMAQAARIAVDRGADMVDINFGCPVKKVAVGQQAGSALMRDEALAARLLDAVVRAVDVPVTLKMRMGWDHA
SLNAPALARIAEQTGIRMVTVHGRTRQQFYNGTADWSFVRQVKDAVSLPVIVNGDIQTVDDARQALALSGADGVMIGRGC
YGRPWFLAQVAQALTTGDIVPDPDLATEKAIVLDHYDMMLSHFGAHPGLRLARKHVAWYSAGLPDSAGFRAAINRVDQAQ
EAIGMISAFYDRHIAAGSHRARRGTAAGGQDAAQAA
>Mature_355_residues
PSMSSEMLRPVDLGGGVVLRTPVILAPMSGVTDLPFRRLARRLGAGLVISEMIASWAMVRENQATLRMAEVADDDGPNAV
QLAGCEPEAMAQAARIAVDRGADMVDINFGCPVKKVAVGQQAGSALMRDEALAARLLDAVVRAVDVPVTLKMRMGWDHAS
LNAPALARIAEQTGIRMVTVHGRTRQQFYNGTADWSFVRQVKDAVSLPVIVNGDIQTVDDARQALALSGADGVMIGRGCY
GRPWFLAQVAQALTTGDIVPDPDLATEKAIVLDHYDMMLSHFGAHPGLRLARKHVAWYSAGLPDSAGFRAAINRVDQAQE
AIGMISAFYDRHIAAGSHRARRGTAAGGQDAAQAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=278, Percent_Identity=30.2158273381295, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=30.7958477508651, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI40807366, Length=269, Percent_Identity=31.9702602230483, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=31.140350877193, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8923374, Length=243, Percent_Identity=30.8641975308642, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=43.217665615142, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=34.1991341991342, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693211, Length=298, Percent_Identity=28.5234899328859, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=285, Percent_Identity=28.4210526315789, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=32.618025751073, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=32.2869955156951, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=289, Percent_Identity=27.681660899654, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323560, Length=251, Percent_Identity=32.2709163346614, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=27.8260869565217, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24585320, Length=251, Percent_Identity=30.6772908366534, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24580595, Length=222, Percent_Identity=31.5315315315315, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19920448, Length=222, Percent_Identity=31.5315315315315, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=30.8035714285714, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=237, Percent_Identity=30.8016877637131, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37941; Mature: 37810

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSMSSEMLRPVDLGGGVVLRTPVILAPMSGVTDLPFRRLARRLGAGLVISEMIASWAMV
CCCCCHHHCCCCCCCCCEEEECCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RENQATLRMAEVADDDGPNAVQLAGCEPEAMAQAARIAVDRGADMVDINFGCPVKKVAVG
HCCCHHEEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHC
QQAGSALMRDEALAARLLDAVVRAVDVPVTLKMRMGWDHASLNAPALARIAEQTGIRMVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEE
VHGRTRQQFYNGTADWSFVRQVKDAVSLPVIVNGDIQTVDDARQALALSGADGVMIGRGC
ECCCHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHEECCCCCEEECCCC
YGRPWFLAQVAQALTTGDIVPDPDLATEKAIVLDHYDMMLSHFGAHPGLRLARKHVAWYS
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHC
AGLPDSAGFRAAINRVDQAQEAIGMISAFYDRHIAAGSHRARRGTAAGGQDAAQAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PSMSSEMLRPVDLGGGVVLRTPVILAPMSGVTDLPFRRLARRLGAGLVISEMIASWAMV
CCCCHHHCCCCCCCCCEEEECCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RENQATLRMAEVADDDGPNAVQLAGCEPEAMAQAARIAVDRGADMVDINFGCPVKKVAVG
HCCCHHEEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHC
QQAGSALMRDEALAARLLDAVVRAVDVPVTLKMRMGWDHASLNAPALARIAEQTGIRMVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEE
VHGRTRQQFYNGTADWSFVRQVKDAVSLPVIVNGDIQTVDDARQALALSGADGVMIGRGC
ECCCHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHEECCCCCEEECCCC
YGRPWFLAQVAQALTTGDIVPDPDLATEKAIVLDHYDMMLSHFGAHPGLRLARKHVAWYS
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHC
AGLPDSAGFRAAINRVDQAQEAIGMISAFYDRHIAAGSHRARRGTAAGGQDAAQAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]