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The map label for this gene is ntrX [H]

Identifier: 162148047

GI number: 162148047

Start: 2320409

End: 2321800

Strand: Reverse

Name: ntrX [H]

Synonym: GDI_2263

Alternate gene names: 162148047

Gene position: 2321800-2320409 (Counterclockwise)

Preceding gene: 162148048

Following gene: 162148046

Centisome position: 58.87

GC content: 69.32

Gene sequence:

>1392_bases
ATGGGGCATGAAATCCTGATCGTCGATGATGAGCCGGACATCCGGCTGCTTGTCGAGGGCATTCTTCGGGACGAAGGCTA
CGAGACCCGCCTGGCAGGGGATTCGGATTCGGCAATCAGCGCCTTTCGGGCCCGCCGGCCGTCTTTGGTCATCCTGGATG
TCTGGCTCCAGGGCTCGCGTCTCGACGGCCTGGGAATCCTCCAGGCCATCCAGGGCGAGGAACCGGTCGTTCCCACCATC
ATGATCTCCGGCCACGGCACGATCGAAACCGCGGTCGCGGCGCTGCAGCACGGCGCCTATGACTTCATCGAAAAGCCCTT
CCAGTCCGACCGGCTGCTGCTGGTGGTCCGCCGCGCGCTGGAGGCATCGCGCCTGGCCCGGGAAAACGCCGAGCTGCGCC
TGCGCGCGGGGCCCGAGGCGATGCTGTACGGCGACAGCCCGGTCATCGCGGGCGTGCGCAACCAGATCGAGCGCGTGGCC
CCCAGCGGCTCGCGCGTGCTGATTTCCGGCGCCGCCGGGGCGGGCAAGGAGGTCGCGGCGCGGATGATCCACGCGCGCTC
GCGCCGGGCCGAAGGGCCGTTCATCGCCCTGAACTGCGCCACCCTGGCCCCCGGCCGGTTCGAGGAGGAACTGTTCGGCA
TCGAGGGGGCGCCGGACGGCACCGGCCGCCGGACCGGCGTGCTGGAACGCGCCCATGGCGGCACCCTGCTGCTGGACGAG
GTTTCGGACATGCCGATCGAAACCCAGGGCAAGATCGTCCGCGCGTTGCAGGACCAGAGCTTCGAGCGCGTGGGCGGGGC
GTCGCGGGTCAAGGTCGATGTCCGCGTGCTGGCCGCGACCAATCGCGACCTGCAGGAAGCGATCGCCGCCGGCCGCTTCC
GCGAGGACCTGTATTACCGCCTGGCGGTGGTGCCGCTGCGCGTGCCCAGCCTGCGCGAACGGCGCGAGGACATTCCCGGC
CTGGCGCGGCTGTTCCTGCGCCGGGCGGCGGAAAACGCGGGCCTGCCGCTGCGCGACCTGTCGGGCGACGCCGTCGCCGC
GCTGCAAAGCTATGACTGGCCGGGCAACGCGCGGGAACTGCGCAACCTGATGGAACGGCTGCTGATCATGATGCCGGGCA
ACGGGTCCGACCTGATCCGTGCCGAGATGCTGCCGCCGTCGGTGGGGCAGGGCGCACCTGCATTGCTGAAATTTGATCCC
GCCGCCGACGTGATGGGCCTGCCGCTGCGCGAGGCACGGGACCTGTTCGAAACCCAGTATCTGCAGGCGCAGCTCCTGCG
TTTTGGCGGCAATATCAGCCGTACCGCCGGCTTCGTCGGCATGGAACGCAGCGCATTGCACCGCAAGTTGAAACAGTTGG
GCGTCACGTCGGAGGAGCGGGGCGCAGGATGA

Upstream 100 bases:

>100_bases
AAGAAAATCCTGGAAGATCATGGTGGTAGCATCCGTCTGGAGGACCGGCCAGACGCAAGGGGAGCGGTTTCGATCTTGAT
ACTGCCTGTAAAGGACGACC

Downstream 100 bases:

>100_bases
GGTTTTGCCCCGCGGGGAAGGGCCTGCTAGACAAGGGCAATCGCATGTTATCGGTACGGAGCGGTACGGCGTGTTCCGCC
CTCGTTCCCGCCCACAGAAA

Product: nitrogen assimilation regulatory protein ntrX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MGHEILIVDDEPDIRLLVEGILRDEGYETRLAGDSDSAISAFRARRPSLVILDVWLQGSRLDGLGILQAIQGEEPVVPTI
MISGHGTIETAVAALQHGAYDFIEKPFQSDRLLLVVRRALEASRLARENAELRLRAGPEAMLYGDSPVIAGVRNQIERVA
PSGSRVLISGAAGAGKEVAARMIHARSRRAEGPFIALNCATLAPGRFEEELFGIEGAPDGTGRRTGVLERAHGGTLLLDE
VSDMPIETQGKIVRALQDQSFERVGGASRVKVDVRVLAATNRDLQEAIAAGRFREDLYYRLAVVPLRVPSLRERREDIPG
LARLFLRRAAENAGLPLRDLSGDAVAALQSYDWPGNARELRNLMERLLIMMPGNGSDLIRAEMLPPSVGQGAPALLKFDP
AADVMGLPLREARDLFETQYLQAQLLRFGGNISRTAGFVGMERSALHRKLKQLGVTSEERGAG

Sequences:

>Translated_463_residues
MGHEILIVDDEPDIRLLVEGILRDEGYETRLAGDSDSAISAFRARRPSLVILDVWLQGSRLDGLGILQAIQGEEPVVPTI
MISGHGTIETAVAALQHGAYDFIEKPFQSDRLLLVVRRALEASRLARENAELRLRAGPEAMLYGDSPVIAGVRNQIERVA
PSGSRVLISGAAGAGKEVAARMIHARSRRAEGPFIALNCATLAPGRFEEELFGIEGAPDGTGRRTGVLERAHGGTLLLDE
VSDMPIETQGKIVRALQDQSFERVGGASRVKVDVRVLAATNRDLQEAIAAGRFREDLYYRLAVVPLRVPSLRERREDIPG
LARLFLRRAAENAGLPLRDLSGDAVAALQSYDWPGNARELRNLMERLLIMMPGNGSDLIRAEMLPPSVGQGAPALLKFDP
AADVMGLPLREARDLFETQYLQAQLLRFGGNISRTAGFVGMERSALHRKLKQLGVTSEERGAG
>Mature_462_residues
GHEILIVDDEPDIRLLVEGILRDEGYETRLAGDSDSAISAFRARRPSLVILDVWLQGSRLDGLGILQAIQGEEPVVPTIM
ISGHGTIETAVAALQHGAYDFIEKPFQSDRLLLVVRRALEASRLARENAELRLRAGPEAMLYGDSPVIAGVRNQIERVAP
SGSRVLISGAAGAGKEVAARMIHARSRRAEGPFIALNCATLAPGRFEEELFGIEGAPDGTGRRTGVLERAHGGTLLLDEV
SDMPIETQGKIVRALQDQSFERVGGASRVKVDVRVLAATNRDLQEAIAAGRFREDLYYRLAVVPLRVPSLRERREDIPGL
ARLFLRRAAENAGLPLRDLSGDAVAALQSYDWPGNARELRNLMERLLIMMPGNGSDLIRAEMLPPSVGQGAPALLKFDPA
ADVMGLPLREARDLFETQYLQAQLLRFGGNISRTAGFVGMERSALHRKLKQLGVTSEERGAG

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=462, Percent_Identity=39.6103896103896, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI1790437, Length=454, Percent_Identity=37.8854625550661, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=35.9743040685225, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1788905, Length=455, Percent_Identity=36.9230769230769, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=38.4615384615385, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789087, Length=401, Percent_Identity=36.4089775561097, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082117, Length=323, Percent_Identity=38.390092879257, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=39.0243902439024, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=38.1381381381381, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=37.2611464968153, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=42.5438596491228, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081858, Length=298, Percent_Identity=32.5503355704698, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=34.5679012345679, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786599, Length=205, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=158, Percent_Identity=28.4810126582279, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50390; Mature: 50258

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHEILIVDDEPDIRLLVEGILRDEGYETRLAGDSDSAISAFRARRPSLVILDVWLQGSR
CCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCC
LDGLGILQAIQGEEPVVPTIMISGHGTIETAVAALQHGAYDFIEKPFQSDRLLLVVRRAL
CCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
EASRLARENAELRLRAGPEAMLYGDSPVIAGVRNQIERVAPSGSRVLISGAAGAGKEVAA
HHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RMIHARSRRAEGPFIALNCATLAPGRFEEELFGIEGAPDGTGRRTGVLERAHGGTLLLDE
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEC
VSDMPIETQGKIVRALQDQSFERVGGASRVKVDVRVLAATNRDLQEAIAAGRFREDLYYR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHEE
LAVVPLRVPSLRERREDIPGLARLFLRRAAENAGLPLRDLSGDAVAALQSYDWPGNAREL
EEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHH
RNLMERLLIMMPGNGSDLIRAEMLPPSVGQGAPALLKFDPAADVMGLPLREARDLFETQY
HHHHHHHEEEECCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
LQAQLLRFGGNISRTAGFVGMERSALHRKLKQLGVTSEERGAG
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GHEILIVDDEPDIRLLVEGILRDEGYETRLAGDSDSAISAFRARRPSLVILDVWLQGSR
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCEEEEEEEECCCC
LDGLGILQAIQGEEPVVPTIMISGHGTIETAVAALQHGAYDFIEKPFQSDRLLLVVRRAL
CCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
EASRLARENAELRLRAGPEAMLYGDSPVIAGVRNQIERVAPSGSRVLISGAAGAGKEVAA
HHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RMIHARSRRAEGPFIALNCATLAPGRFEEELFGIEGAPDGTGRRTGVLERAHGGTLLLDE
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEEC
VSDMPIETQGKIVRALQDQSFERVGGASRVKVDVRVLAATNRDLQEAIAAGRFREDLYYR
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHEE
LAVVPLRVPSLRERREDIPGLARLFLRRAAENAGLPLRDLSGDAVAALQSYDWPGNAREL
EEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHH
RNLMERLLIMMPGNGSDLIRAEMLPPSVGQGAPALLKFDPAADVMGLPLREARDLFETQY
HHHHHHHEEEECCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHH
LQAQLLRFGGNISRTAGFVGMERSALHRKLKQLGVTSEERGAG
HHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]