Click here to switch to the map view.

The map label for this gene is purA

Identifier: 162148039

GI number: 162148039

Start: 2312065

End: 2313354

Strand: Reverse

Name: purA

Synonym: GDI_2255

Alternate gene names: 162148039

Gene position: 2313354-2312065 (Counterclockwise)

Preceding gene: 162148040

Following gene: 162148036

Centisome position: 58.65

GC content: 69.07

Gene sequence:

>1290_bases
ATGTCCAACGTCACCGTCATCGGCGCCCAGTGGGGTGACGAGGGAAAGGGCAAGATCGTCGACTGGCTGGCCAGCCGCGC
CGATATCGTCGTCCGCTTCCAGGGCGGTCACAATGCCGGCCATACGCTGGTGGTGGGCAGCCAGACCTACAAGCTGTCGC
TGCTGCCGTCCGGCCTGGTCCGAGGCAAGATGGGGGTGATCGGCAACGGCGTCGTCGTGGACCCCGAGGCGCTGCTGGCG
GAAATCGACCGGGTGAGCGCGCAGGGGCTGGAAGTATCGCCGCGGACGCTGCGCATCGCTGAAAACGTGCCGCTGATCCT
GCCCGTCCACGGCGCCATCGATCGCGCGCGTGAAGCCGCGCGCGGCGACCGCAAGATCGGCACCACGGGCCGCGGCATCG
GCCCGGCCTACGAGGACAAGGTGGCACGCCGCGCCATCCGCCTGTGCGACCTGGCCGAGCCCGAGACGCTGGACTGGAAG
CTGGACGAACTGCTGCTGCACCACAACACGCTGCTGGCCGGCCTGGGGGCCGAGACCTTTACCAAGGACGCCCTGCTGGC
CCACCTGACCGCGCTGGCGCCGCGCGTGCTGCCCTACATGGATACGGTGTGGGACCTGCTGGACGATGCGCGCCGCGCCG
GACGGCGAATCCTGTTCGAAGGTGCGCAGGCGGTGATGCTGGACGTCGATCACGGCACCTATCCGTTCGTCACCAGTTCC
AACACCATCGCGGCCAATGCCGCGACCGGCAGCGGCGTGGGGCCGGGCAGCGTCGGCTTCGTGCTGGGTATCGCCAAGGC
CTATTCGACCCGCGTGGGCGAGGGGCCGTTCCCCAGCGAACTGCATGACGACATGGGCCGCACCCTGGGCGAGCGCGGGC
ATGAATTCGGCACGGTGACGGGGCGCCCCCGCCGCTGCGGCTGGTTCGACGCCATGCTGGTCCGCCGCGCGGTGCGCGTG
GGCGGCGTCAACGGTCTGGCCCTGACCAAGCTGGACGTGCTGGATGGCCTGCCCGAGATCCGCGTGTGCGTCGGCTATGA
ACTGGACGGCCAGGAAATCCGCAGCTTCCCGTCCTCGCCGGCGGCGCAGGCCCGCGTCAAGCCGATCTACGAGACGATGG
AAGGCTGGACCGATACCACCTACGGCGCGCGGTCCTGGGCGGACCTGCCGGCGCAGGCGATCAAATATGTCCGCCGGATC
GAGGAACTGGTCGAGGCACCGGTGACCCTGCTGTCCACCAGTCCGGAACGCGACGATACGATCCTGATGCGCGACCCGTT
CGAGAACTGA

Upstream 100 bases:

>100_bases
CGATGATCGCGGCCGGCGACGGAATCGTGGCGTTAGACTGACACATTTGTTAAAGCCGGGCGCCATCTGCCGCCCGTCCC
GTTTTTCCAAGGAGCACCTT

Downstream 100 bases:

>100_bases
CGATCCACCCGGAAACGGGAAACGGAAGAGGGGGAGGCGCTTCAACGCCCCCCGTCATTCTCAGTTTGCGGCGTGGGCTT
CGCGATGGGCCTCGACTTCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSNVTVIGAQWGDEGKGKIVDWLASRADIVVRFQGGHNAGHTLVVGSQTYKLSLLPSGLVRGKMGVIGNGVVVDPEALLA
EIDRVSAQGLEVSPRTLRIAENVPLILPVHGAIDRAREAARGDRKIGTTGRGIGPAYEDKVARRAIRLCDLAEPETLDWK
LDELLLHHNTLLAGLGAETFTKDALLAHLTALAPRVLPYMDTVWDLLDDARRAGRRILFEGAQAVMLDVDHGTYPFVTSS
NTIAANAATGSGVGPGSVGFVLGIAKAYSTRVGEGPFPSELHDDMGRTLGERGHEFGTVTGRPRRCGWFDAMLVRRAVRV
GGVNGLALTKLDVLDGLPEIRVCVGYELDGQEIRSFPSSPAAQARVKPIYETMEGWTDTTYGARSWADLPAQAIKYVRRI
EELVEAPVTLLSTSPERDDTILMRDPFEN

Sequences:

>Translated_429_residues
MSNVTVIGAQWGDEGKGKIVDWLASRADIVVRFQGGHNAGHTLVVGSQTYKLSLLPSGLVRGKMGVIGNGVVVDPEALLA
EIDRVSAQGLEVSPRTLRIAENVPLILPVHGAIDRAREAARGDRKIGTTGRGIGPAYEDKVARRAIRLCDLAEPETLDWK
LDELLLHHNTLLAGLGAETFTKDALLAHLTALAPRVLPYMDTVWDLLDDARRAGRRILFEGAQAVMLDVDHGTYPFVTSS
NTIAANAATGSGVGPGSVGFVLGIAKAYSTRVGEGPFPSELHDDMGRTLGERGHEFGTVTGRPRRCGWFDAMLVRRAVRV
GGVNGLALTKLDVLDGLPEIRVCVGYELDGQEIRSFPSSPAAQARVKPIYETMEGWTDTTYGARSWADLPAQAIKYVRRI
EELVEAPVTLLSTSPERDDTILMRDPFEN
>Mature_428_residues
SNVTVIGAQWGDEGKGKIVDWLASRADIVVRFQGGHNAGHTLVVGSQTYKLSLLPSGLVRGKMGVIGNGVVVDPEALLAE
IDRVSAQGLEVSPRTLRIAENVPLILPVHGAIDRAREAARGDRKIGTTGRGIGPAYEDKVARRAIRLCDLAEPETLDWKL
DELLLHHNTLLAGLGAETFTKDALLAHLTALAPRVLPYMDTVWDLLDDARRAGRRILFEGAQAVMLDVDHGTYPFVTSSN
TIAANAATGSGVGPGSVGFVLGIAKAYSTRVGEGPFPSELHDDMGRTLGERGHEFGTVTGRPRRCGWFDAMLVRRAVRVG
GVNGLALTKLDVLDGLPEIRVCVGYELDGQEIRSFPSSPAAQARVKPIYETMEGWTDTTYGARSWADLPAQAIKYVRRIE
ELVEAPVTLLSTSPERDDTILMRDPFEN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=42.8904428904429, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=40.7494145199063, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=40.4040404040404, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=52.3255813953488, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=41.5704387990762, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=41.5704387990762, Blast_Score=322, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=38.2830626450116, Blast_Score=303, Evalue=4e-83,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=41.7661097852029, Blast_Score=321, Evalue=8e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_GLUDA (A9HLQ1)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602500.1
- RefSeq:   YP_002274886.1
- ProteinModelPortal:   A9HLQ1
- SMR:   A9HLQ1
- GeneID:   5792257
- GeneID:   6973870
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0475
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46358; Mature: 46227

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVTVIGAQWGDEGKGKIVDWLASRADIVVRFQGGHNAGHTLVVGSQTYKLSLLPSGLV
CCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCHHH
RGKMGVIGNGVVVDPEALLAEIDRVSAQGLEVSPRTLRIAENVPLILPVHGAIDRAREAA
CCCCCEECCCEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHH
RGDRKIGTTGRGIGPAYEDKVARRAIRLCDLAEPETLDWKLDELLLHHNTLLAGLGAETF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
TKDALLAHLTALAPRVLPYMDTVWDLLDDARRAGRRILFEGAQAVMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCEEECC
NTIAANAATGSGVGPGSVGFVLGIAKAYSTRVGEGPFPSELHDDMGRTLGERGHEFGTVT
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAMLVRRAVRVGGVNGLALTKLDVLDGLPEIRVCVGYELDGQEIRSFPSSP
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCEEEEEECCCCHHHHHCCCCCC
AAQARVKPIYETMEGWTDTTYGARSWADLPAQAIKYVRRIEELVEAPVTLLSTSPERDDT
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCCE
ILMRDPFEN
EEECCCCCC
>Mature Secondary Structure 
SNVTVIGAQWGDEGKGKIVDWLASRADIVVRFQGGHNAGHTLVVGSQTYKLSLLPSGLV
CCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEECCCHHH
RGKMGVIGNGVVVDPEALLAEIDRVSAQGLEVSPRTLRIAENVPLILPVHGAIDRAREAA
CCCCCEECCCEEECHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHHH
RGDRKIGTTGRGIGPAYEDKVARRAIRLCDLAEPETLDWKLDELLLHHNTLLAGLGAETF
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
TKDALLAHLTALAPRVLPYMDTVWDLLDDARRAGRRILFEGAQAVMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEECCCCCCCEEECC
NTIAANAATGSGVGPGSVGFVLGIAKAYSTRVGEGPFPSELHDDMGRTLGERGHEFGTVT
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAMLVRRAVRVGGVNGLALTKLDVLDGLPEIRVCVGYELDGQEIRSFPSSP
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCEEEEEECCCCHHHHHCCCCCC
AAQARVKPIYETMEGWTDTTYGARSWADLPAQAIKYVRRIEELVEAPVTLLSTSPERDDT
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEECCCCCCCCE
ILMRDPFEN
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA