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The map label for this gene is pucI [H]

Identifier: 162147791

GI number: 162147791

Start: 2045784

End: 2047244

Strand: Direct

Name: pucI [H]

Synonym: GDI_2007

Alternate gene names: 162147791

Gene position: 2045784-2047244 (Clockwise)

Preceding gene: 162147790

Following gene: 162147796

Centisome position: 51.87

GC content: 68.51

Gene sequence:

>1461_bases
ATGACAGACACCCCGCCCCCTGCGACGGCCGCCGGCTACGATCCCGGCCTGTATAACGCGGACCTGGCCCCGGTTCCGGC
CGAGCGGCGGGACTGGAGCTGGGTGAACATGGCCACGGTGTGGATGGGCATGGTCCACAACATCGTGGTGTACGAGGCCG
CGTCGGGCCTGATGGCGCTGGGCCTGTCGGCCTGGGAATGCCTGGAGGTCGTGGCCGTCGCCTACATGGTGCTGTTCCTG
GCCATGTGGTTCAACGCCCGTGCCGGCACGCGCTACGGCATTCCGTTCTGCGTGCTGATCCGCTCGGCCTTCGGGCCGTA
CGGCGCGCAGCTTCCGGTCGTGCTGCGCGGATTCTGCGCCATCTTCTGGTTTTCGGTGCAGGCCTATGCGGCGGCGCAGG
CGGTGGATGCGGTGCTGTCCACCCTCAGCCCCGCCTGGGCGTCGATGACCCCGTCCCTGCTGGGCATGCAGGCGCGGATG
TGGCTGGCCATGGCCATGGTCTGGGCGCTGCATGCCTGGATCGCCAGCCACGGCGTGCACCGGATCCGCAATTTCGAGCT
TGTCGCCGGGCCGCTGGTCATCATCGTGGGCCTGCTGGCCACGATCTGGGGGCTGCGCGTCGGCCATGGGCTGGGGCCGC
TGTTCGCGCAGCCGTCGCACCTGCATGGGGCGGCATTCTGGTCCACCTTCGCCATGGGGGTGACCGGCATGATCGGCATG
TGGGCCACCTTGACCGTCAATATTCCGGACCTGTCGCGGTTCGTACGCTCGGAACGCGACCAGGTGGTCGGCCAGGCGAT
CGGCCTGCCGATCACGGCGCTGGTCTTCACGCCGATGGGCATCATCACCACGTCGGCCACAATCATCTTGTTCGGCCACC
CGATCTGGAACCCGGTGGACCTGCTTCTGGCGCTGAACCATCCTGTCGTGACCGTCCTGGGCGGGGCCACGCTGGTGCTG
GCGACGCTGTCGGTCAACGTCGTCGCGAACATCATGCCCGCCTGCTACGACCTGGTGAACCTGATGCCCCGGCGGCTGGA
CTTCAACCGGGCGTCGCGGCTGGTCCTGGTGCTGGGCGTGTTCTTCATGCCCTGGCTGTGGTTCAACGAAGCCGCCGGCA
TCTATCGCGTGCTGGACCTGATCAGCGGCCTTCTGGGCCCGGTGACGGGGATCATGCTGGCCGATTTCTACATCGTGCGG
CGGCAGGTGCTGGATGTACCGGCGCTGTACCGGCATGGCGGCCGGTACGACGGCCGAAACGGATGGAACGTCCCGGCCCT
GGCCGCCTTCGCCGCCGGCGGCGCGGTGGCGTCGGCCGGCCACGTCGTGCCGGGTCTGGCCGGCCTGAATACGGTCGCGT
GGTTCGTGGGCGTCGCCATCGGTGCCGGGCTCTATCTGGCGTTGTCGCCCCGCCGGGATGCGCACCCGGCGGAAGACGCC
GCAACGCTGTCAAACGCGTGA

Upstream 100 bases:

>100_bases
TCGCCGCCGCTGATCATCGAAAAATCGCAGATCGACCAGGTGTTCGGCACCCTGGCCGACATCATCCACGCCGTCGAATA
GCGCGGAACGCGAGGCCCGA

Downstream 100 bases:

>100_bases
CGCCGCCCAGTCAATTCGGGCCCGGTCAATCCGGCAGCATGGCCTGCTGGTAGGGTTGCTCCTGGATGGCGCGTCCGGGT
TCGAAATGGCCGATCCGGCA

Product: allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTDTPPPATAAGYDPGLYNADLAPVPAERRDWSWVNMATVWMGMVHNIVVYEAASGLMALGLSAWECLEVVAVAYMVLFL
AMWFNARAGTRYGIPFCVLIRSAFGPYGAQLPVVLRGFCAIFWFSVQAYAAAQAVDAVLSTLSPAWASMTPSLLGMQARM
WLAMAMVWALHAWIASHGVHRIRNFELVAGPLVIIVGLLATIWGLRVGHGLGPLFAQPSHLHGAAFWSTFAMGVTGMIGM
WATLTVNIPDLSRFVRSERDQVVGQAIGLPITALVFTPMGIITTSATIILFGHPIWNPVDLLLALNHPVVTVLGGATLVL
ATLSVNVVANIMPACYDLVNLMPRRLDFNRASRLVLVLGVFFMPWLWFNEAAGIYRVLDLISGLLGPVTGIMLADFYIVR
RQVLDVPALYRHGGRYDGRNGWNVPALAAFAAGGAVASAGHVVPGLAGLNTVAWFVGVAIGAGLYLALSPRRDAHPAEDA
ATLSNA

Sequences:

>Translated_486_residues
MTDTPPPATAAGYDPGLYNADLAPVPAERRDWSWVNMATVWMGMVHNIVVYEAASGLMALGLSAWECLEVVAVAYMVLFL
AMWFNARAGTRYGIPFCVLIRSAFGPYGAQLPVVLRGFCAIFWFSVQAYAAAQAVDAVLSTLSPAWASMTPSLLGMQARM
WLAMAMVWALHAWIASHGVHRIRNFELVAGPLVIIVGLLATIWGLRVGHGLGPLFAQPSHLHGAAFWSTFAMGVTGMIGM
WATLTVNIPDLSRFVRSERDQVVGQAIGLPITALVFTPMGIITTSATIILFGHPIWNPVDLLLALNHPVVTVLGGATLVL
ATLSVNVVANIMPACYDLVNLMPRRLDFNRASRLVLVLGVFFMPWLWFNEAAGIYRVLDLISGLLGPVTGIMLADFYIVR
RQVLDVPALYRHGGRYDGRNGWNVPALAAFAAGGAVASAGHVVPGLAGLNTVAWFVGVAIGAGLYLALSPRRDAHPAEDA
ATLSNA
>Mature_485_residues
TDTPPPATAAGYDPGLYNADLAPVPAERRDWSWVNMATVWMGMVHNIVVYEAASGLMALGLSAWECLEVVAVAYMVLFLA
MWFNARAGTRYGIPFCVLIRSAFGPYGAQLPVVLRGFCAIFWFSVQAYAAAQAVDAVLSTLSPAWASMTPSLLGMQARMW
LAMAMVWALHAWIASHGVHRIRNFELVAGPLVIIVGLLATIWGLRVGHGLGPLFAQPSHLHGAAFWSTFAMGVTGMIGMW
ATLTVNIPDLSRFVRSERDQVVGQAIGLPITALVFTPMGIITTSATIILFGHPIWNPVDLLLALNHPVVTVLGGATLVLA
TLSVNVVANIMPACYDLVNLMPRRLDFNRASRLVLVLGVFFMPWLWFNEAAGIYRVLDLISGLLGPVTGIMLADFYIVRR
QVLDVPALYRHGGRYDGRNGWNVPALAAFAAGGAVASAGHVVPGLAGLNTVAWFVGVAIGAGLYLALSPRRDAHPAEDAA
TLSNA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=472, Percent_Identity=27.7542372881356, Blast_Score=201, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6319495, Length=484, Percent_Identity=27.2727272727273, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6322219, Length=480, Percent_Identity=26.875, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319429, Length=475, Percent_Identity=24, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324766, Length=461, Percent_Identity=23.644251626898, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323266, Length=459, Percent_Identity=22.4400871459695, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=461, Percent_Identity=21.6919739696312, Blast_Score=93, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 52140; Mature: 52009

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCC
>Mature Secondary Structure 
TDTPPPATAAGYDPGLYNADLAPVPAERRDWSWVNMATVWMGMVHNIVVYEAASGLMAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSAWECLEVVAVAYMVLFLAMWFNARAGTRYGIPFCVLIRSAFGPYGAQLPVVLRGFCA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
IFWFSVQAYAAAQAVDAVLSTLSPAWASMTPSLLGMQARMWLAMAMVWALHAWIASHGVH
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIRNFELVAGPLVIIVGLLATIWGLRVGHGLGPLFAQPSHLHGAAFWSTFAMGVTGMIGM
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
WATLTVNIPDLSRFVRSERDQVVGQAIGLPITALVFTPMGIITTSATIILFGHPIWNPVD
HEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCCEEEEECCCCCCHHH
LLLALNHPVVTVLGGATLVLATLSVNVVANIMPACYDLVNLMPRRLDFNRASRLVLVLGV
HHHHCCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FFMPWLWFNEAAGIYRVLDLISGLLGPVTGIMLADFYIVRRQVLDVPALYRHGGRYDGRN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCC
GWNVPALAAFAAGGAVASAGHVVPGLAGLNTVAWFVGVAIGAGLYLALSPRRDAHPAEDA
CCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHH
ATLSNA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]