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The map label for this gene is sseA [H]

Identifier: 162147752

GI number: 162147752

Start: 2001487

End: 2002407

Strand: Direct

Name: sseA [H]

Synonym: GDI_1968

Alternate gene names: 162147752

Gene position: 2001487-2002407 (Clockwise)

Preceding gene: 162147749

Following gene: 162147753

Centisome position: 50.75

GC content: 72.53

Gene sequence:

>921_bases
ATGACAGGGGCTGTACCGGCCGCCGGGGACGTCCCCGGACCACGGAACCGTGAAGGGATTCCTGCCATGCATCCGCTGAT
TTCGCCGGCCCAGCTACAGGAGCGCCTGGCCGCGCCCGACCTGGTGGTCCTGGACGCCACGATGGCATTGCCGGGCGAAA
CGTTCGATCCCGCCCGGTGCTTCGCGCAGGCTCATATTCCCGGCGCCCGCCGGTTCGATATCGAGCTGTTCTCGGACCCG
GATTCCCCGCTGCCGCACATGATCCCGACCCAGGCGCGGTTCGAGCATCTGGCGCGGGGCCTGGGCCTGTCGGACGGGGC
GCACGTCGTCTTCTACGACCAGGGCAATGCGGCCTCGGCCGCGCGGGGGTGGTGGCTTGCCGGCCTGTTCGGGCTGGACC
GGGTCCAGGTGCTGGATGGCGGACTGCCCGCCTGGCAGCGCGCCGGCGGCCCGGTCGAGCAAGGTGAGGAAGGACCTGCG
GCGGCAGGGGGATTCCGTGCCGCACCGCGCTTTGCCCGGGTCAGCGGCCTGGGCGACATGCGGGCCCACGTGGGGCGGGG
CGACCGGCTGATCCTGGACGCCCGAGCGCAGGCCCGCTTTGCCGGAACGGCGGCCGAACCGCGTCCGGGCCTGGCGTCGG
GCCATATGCCCGGGGCACGCAACCTGCCGTTCGGCACGCTGCTCGATGCCAATCGCGCGTTCCTGCCGCCCGCGGCGCTG
CGCGATCGCTTCGCCCATGTGGGCGTGACCGATGGGCGGCCGGTCGTCACCACCTGCGGCTCCGGCGTATCGGCCGCGGT
GCTGTCCCTGGGGCTGGCCATCTGCGGGCGAACCGGCGACTCGCTTTATGACGGATCGTGGGCCGAATGGGGGTCCACCC
CGGGCGCGCCGGTGGAAACGGGCGAGGGCACATCCCCATGA

Upstream 100 bases:

>100_bases
GTGCGATGATGTGGCAGAGCGCCCTGACGGTAAAGGCCTACCCGGGAGCGGGCGCCATCACATGATAGGCCCGGTTGATC
CGTGCGGGGCCGCTCCGGTA

Downstream 100 bases:

>100_bases
CCGACCGTCAGGCCCTGCATCATGCCGTCTCCCGCGGCTGGCGCCATATGGGCACCACGCTGGTCCATCTGGCGCGCGAC
CTGCCGCCGGCGGCGGAGGG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTGAVPAAGDVPGPRNREGIPAMHPLISPAQLQERLAAPDLVVLDATMALPGETFDPARCFAQAHIPGARRFDIELFSDP
DSPLPHMIPTQARFEHLARGLGLSDGAHVVFYDQGNAASAARGWWLAGLFGLDRVQVLDGGLPAWQRAGGPVEQGEEGPA
AAGGFRAAPRFARVSGLGDMRAHVGRGDRLILDARAQARFAGTAAEPRPGLASGHMPGARNLPFGTLLDANRAFLPPAAL
RDRFAHVGVTDGRPVVTTCGSGVSAAVLSLGLAICGRTGDSLYDGSWAEWGSTPGAPVETGEGTSP

Sequences:

>Translated_306_residues
MTGAVPAAGDVPGPRNREGIPAMHPLISPAQLQERLAAPDLVVLDATMALPGETFDPARCFAQAHIPGARRFDIELFSDP
DSPLPHMIPTQARFEHLARGLGLSDGAHVVFYDQGNAASAARGWWLAGLFGLDRVQVLDGGLPAWQRAGGPVEQGEEGPA
AAGGFRAAPRFARVSGLGDMRAHVGRGDRLILDARAQARFAGTAAEPRPGLASGHMPGARNLPFGTLLDANRAFLPPAAL
RDRFAHVGVTDGRPVVTTCGSGVSAAVLSLGLAICGRTGDSLYDGSWAEWGSTPGAPVETGEGTSP
>Mature_305_residues
TGAVPAAGDVPGPRNREGIPAMHPLISPAQLQERLAAPDLVVLDATMALPGETFDPARCFAQAHIPGARRFDIELFSDPD
SPLPHMIPTQARFEHLARGLGLSDGAHVVFYDQGNAASAARGWWLAGLFGLDRVQVLDGGLPAWQRAGGPVEQGEEGPAA
AGGFRAAPRFARVSGLGDMRAHVGRGDRLILDARAQARFAGTAAEPRPGLASGHMPGARNLPFGTLLDANRAFLPPAALR
DRFAHVGVTDGRPVVTTCGSGVSAAVLSLGLAICGRTGDSLYDGSWAEWGSTPGAPVETGEGTSP

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=271, Percent_Identity=39.4833948339483, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI61835204, Length=271, Percent_Identity=39.4833948339483, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI194473681, Length=271, Percent_Identity=39.4833948339483, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI17402865, Length=272, Percent_Identity=35.6617647058824, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI87082121, Length=270, Percent_Identity=41.1111111111111, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17561888, Length=295, Percent_Identity=23.728813559322, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71997283, Length=298, Percent_Identity=24.8322147651007, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=239, Percent_Identity=27.6150627615063, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559150, Length=251, Percent_Identity=25.4980079681275, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17543836, Length=231, Percent_Identity=28.1385281385281, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543838, Length=231, Percent_Identity=28.1385281385281, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=288, Percent_Identity=30.9027777777778, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31708; Mature: 31577

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAVPAAGDVPGPRNREGIPAMHPLISPAQLQERLAAPDLVVLDATMALPGETFDPARC
CCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEEHEECCCCCCCHHHH
FAQAHIPGARRFDIELFSDPDSPLPHMIPTQARFEHLARGLGLSDGAHVVFYDQGNAASA
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
ARGWWLAGLFGLDRVQVLDGGLPAWQRAGGPVEQGEEGPAAAGGFRAAPRFARVSGLGDM
HCCHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHH
RAHVGRGDRLILDARAQARFAGTAAEPRPGLASGHMPGARNLPFGTLLDANRAFLPPAAL
HHHCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHH
RDRFAHVGVTDGRPVVTTCGSGVSAAVLSLGLAICGRTGDSLYDGSWAEWGSTPGAPVET
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
GEGTSP
CCCCCC
>Mature Secondary Structure 
TGAVPAAGDVPGPRNREGIPAMHPLISPAQLQERLAAPDLVVLDATMALPGETFDPARC
CCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCEEEEEEHEECCCCCCCHHHH
FAQAHIPGARRFDIELFSDPDSPLPHMIPTQARFEHLARGLGLSDGAHVVFYDQGNAASA
HHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCH
ARGWWLAGLFGLDRVQVLDGGLPAWQRAGGPVEQGEEGPAAAGGFRAAPRFARVSGLGDM
HCCHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHH
RAHVGRGDRLILDARAQARFAGTAAEPRPGLASGHMPGARNLPFGTLLDANRAFLPPAAL
HHHCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHH
RDRFAHVGVTDGRPVVTTCGSGVSAAVLSLGLAICGRTGDSLYDGSWAEWGSTPGAPVET
HHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
GEGTSP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]