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The map label for this gene is lipA [H]

Identifier: 162147727

GI number: 162147727

Start: 1978916

End: 1979905

Strand: Direct

Name: lipA [H]

Synonym: GDI_1943

Alternate gene names: 162147727

Gene position: 1978916-1979905 (Clockwise)

Preceding gene: 162147726

Following gene: 162147728

Centisome position: 50.17

GC content: 66.87

Gene sequence:

>990_bases
ATGCCAACGCGCCTGTTGATCGACCATAGAAAGGGCGGGGTTTCCGTCCGCCATCCGGAAAAGGCGCACCGGCCGGACAA
CCCGATCGCGCGCAAGCCCGACTGGATCCGCGTCAGGGCGCCGAACCATCCGGTCTACCAGGAAACCCGTGCCCTGATGC
GCGAGCAGAAGCTGGTGACGGTGTGCGAGGAAGCGGCCTGCCCGAATATCGGCGAATGCTGGTCGCAGCGGCATGCCACC
ATGATGATCATGGGCGAGATCTGCACCCGCGCCTGCGCCTTCTGCAACGTGACCACGGGCATGCCCAACCCCCTGGACGC
CGACGAGCCTGCCCGCGTGGGTGACGCGGTGGCCAAGCTGGGGCTGCGGCATGTGGTGATCACCTCGGTCGATCGCGACG
ACCTGCCCGATGGCGGGGCTGCGCATTTCGCCCGGGTCATCGCCGCGATCCGCGCGCAGGCGCCCGAGACGACGATCGAG
ATCCTGACCCCCGATTTCCTGCGCAAGCGCGGCGCGCTGGAGGTCGTGGTGTCGGCGCGTCCCGATGTCTTCAATCACAA
TCTGGAAACCGTGCCGCGCCTGTATCCGGGCATCCGGCCCGGCGCGCGCTATTACCAGTCCCTGCGCCTGCTGGACGATG
TGAAGCGCCTGGACCCGTCGATCTTCACCAAATCCGGCCTGATGGTCGGACTGGGCGAGGAACGGGCGGAAATCCTGCAG
GTCATGGACGATCTGCGCGTGGCCGATGTCGATTTCATCACCATGGGGCAGTACCTGCAGCCGACGGTGAAGCATGCGGC
GGTCCGGGATTTCGTCACCCCCGACGAATTTGCCGATTATGCCGCGATGGCGCGCGCCAAGGGCTTCCTGCAGGTCAGCG
CCTCGCCCCTGACAAGGTCGTCCTATCACGCCGATTCCGACTTCGCGGAACTGCGCGCCGCGCGCGAGGCCCGACTGGCC
GCATCGCGCGTCGGGACGGAGACCCCCTGA

Upstream 100 bases:

>100_bases
GCTTGTGATCGCGGGCGGGCGGGCAAGGGGGATGTTTGCTTGACGCGTTCCCCGCAACGTTCCACTTCTGCTTGTCTGCT
GTTTTTCTGGGTTTGCCGCC

Downstream 100 bases:

>100_bases
TGCCGACACATGCGGAACGCCGTCTGATTGCCTATTCGGTGGAGCAATTGTTCGACCTGGTGGCCGATGTCGGCAAATAC
CCGCATTTCCTGCCCTGGTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MPTRLLIDHRKGGVSVRHPEKAHRPDNPIARKPDWIRVRAPNHPVYQETRALMREQKLVTVCEEAACPNIGECWSQRHAT
MMIMGEICTRACAFCNVTTGMPNPLDADEPARVGDAVAKLGLRHVVITSVDRDDLPDGGAAHFARVIAAIRAQAPETTIE
ILTPDFLRKRGALEVVVSARPDVFNHNLETVPRLYPGIRPGARYYQSLRLLDDVKRLDPSIFTKSGLMVGLGEERAEILQ
VMDDLRVADVDFITMGQYLQPTVKHAAVRDFVTPDEFADYAAMARAKGFLQVSASPLTRSSYHADSDFAELRAAREARLA
ASRVGTETP

Sequences:

>Translated_329_residues
MPTRLLIDHRKGGVSVRHPEKAHRPDNPIARKPDWIRVRAPNHPVYQETRALMREQKLVTVCEEAACPNIGECWSQRHAT
MMIMGEICTRACAFCNVTTGMPNPLDADEPARVGDAVAKLGLRHVVITSVDRDDLPDGGAAHFARVIAAIRAQAPETTIE
ILTPDFLRKRGALEVVVSARPDVFNHNLETVPRLYPGIRPGARYYQSLRLLDDVKRLDPSIFTKSGLMVGLGEERAEILQ
VMDDLRVADVDFITMGQYLQPTVKHAAVRDFVTPDEFADYAAMARAKGFLQVSASPLTRSSYHADSDFAELRAAREARLA
ASRVGTETP
>Mature_328_residues
PTRLLIDHRKGGVSVRHPEKAHRPDNPIARKPDWIRVRAPNHPVYQETRALMREQKLVTVCEEAACPNIGECWSQRHATM
MIMGEICTRACAFCNVTTGMPNPLDADEPARVGDAVAKLGLRHVVITSVDRDDLPDGGAAHFARVIAAIRAQAPETTIEI
LTPDFLRKRGALEVVVSARPDVFNHNLETVPRLYPGIRPGARYYQSLRLLDDVKRLDPSIFTKSGLMVGLGEERAEILQV
MDDLRVADVDFITMGQYLQPTVKHAAVRDFVTPDEFADYAAMARAKGFLQVSASPLTRSSYHADSDFAELRAAREARLAA
SRVGTETP

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=43.2432432432432, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=45.7142857142857, Blast_Score=227, Evalue=9e-60,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=51.9713261648746, Blast_Score=293, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=252, Percent_Identity=47.6190476190476, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324770, Length=298, Percent_Identity=41.6107382550336, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=46.5277777777778, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36482; Mature: 36351

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTRLLIDHRKGGVSVRHPEKAHRPDNPIARKPDWIRVRAPNHPVYQETRALMREQKLVT
CCCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VCEEAACPNIGECWSQRHATMMIMGEICTRACAFCNVTTGMPNPLDADEPARVGDAVAKL
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
GLRHVVITSVDRDDLPDGGAAHFARVIAAIRAQAPETTIEILTPDFLRKRGALEVVVSAR
CHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCEEEEEECC
PDVFNHNLETVPRLYPGIRPGARYYQSLRLLDDVKRLDPSIFTKSGLMVGLGEERAEILQ
CCHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHEECCCCEEECCHHHHHHHH
VMDDLRVADVDFITMGQYLQPTVKHAAVRDFVTPDEFADYAAMARAKGFLQVSASPLTRS
HHHHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCC
SYHADSDFAELRAAREARLAASRVGTETP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PTRLLIDHRKGGVSVRHPEKAHRPDNPIARKPDWIRVRAPNHPVYQETRALMREQKLVT
CCCEEEECCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
VCEEAACPNIGECWSQRHATMMIMGEICTRACAFCNVTTGMPNPLDADEPARVGDAVAKL
HHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
GLRHVVITSVDRDDLPDGGAAHFARVIAAIRAQAPETTIEILTPDFLRKRGALEVVVSAR
CHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCEEEEEECC
PDVFNHNLETVPRLYPGIRPGARYYQSLRLLDDVKRLDPSIFTKSGLMVGLGEERAEILQ
CCHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCHHHEECCCCEEECCHHHHHHHH
VMDDLRVADVDFITMGQYLQPTVKHAAVRDFVTPDEFADYAAMARAKGFLQVSASPLTRS
HHHHHHHHCCCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCC
SYHADSDFAELRAAREARLAASRVGTETP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA