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The map label for this gene is ispG

Identifier: 162147697

GI number: 162147697

Start: 1947483

End: 1948631

Strand: Direct

Name: ispG

Synonym: GDI_1913

Alternate gene names: 162147697

Gene position: 1947483-1948631 (Clockwise)

Preceding gene: 162147695

Following gene: 162147698

Centisome position: 49.38

GC content: 65.8

Gene sequence:

>1149_bases
ATGAGCAGCTATCGTCCCTATCAGTCCATCGAGCGCCGCAAGTCGCGGCAGATCCATGTCGGCAAGGTTCCGGTCGGCGG
CGACGCACCCATTTCGGTGCAGACCATGACCAATACCCTGACGACGGATGCCGAAGCGACGATCGCGCAGATCCGCAGGG
CCGAACTGGCGGGCGTCGATATCGTGCGCGTCTCCTGCCCCGACGAGGAAAGCACCGCCGCGCTGGCCGAGATCGTGCGC
GAGGTCAACGTGCCGATCGTTGCCGACATCCATTTCCACTACAAGCGCGCGATCGAGGCCGCCCAGGCCGGCGCGGCCTG
CCTGCGCATCAATCCCGGCAATATCGGCAGCGCCGAGCGTGTGCGTGAGGTCGTGAAGGCCGCGCGTGAGCATGGCTGCT
CGATCCGCATCGGGGTGAATGCCGGGTCGCTGGAAAAGCACCTGCTGGAAAAATACGGCGAACCCAACCCCGACGCGCTG
GTCGAAAGTGCGCTGGAACACGCCAAGATCCTGCAGGATCACGATTTCCATGAATTCAAGATCAGCGTGAAGGCGTCGGA
CGTCTTCCTGGCCGTGGCCGCCTACCAGCAACTGGCCGAGGTCTGCGACCATCCGCTGCATATCGGCATCACCGAGGCGG
GCAGCAAGCGCGCCGGCACGGTCAAGTCCTCGATCGGCCTGGGCAACCTGCTGTGGGCCGGCGTGGGCGATACCATGCGC
GTCTCGCTGTCGGCGGACCCCGAGGAAGAGGTCCTGGTGGGCTGGGATATCCTGAAATCCCTGGGCCTGCGCCATCGCGG
CGTGAAGATCATTTCCTGCCCCTCATGCGCGCGGCAGGGGTTCAACGTCATCCAGACCGTGCAGACCCTGGAGGAGCGCC
TGGCGCACATCCAGACGCCGCTGACGCTGTCCATCATCGGCTGCGTGGTCAACGGCCCCGGCGAGGCGCTGATGACCGAT
ATCGGCGTGACCGGCGGCGGATCGGGCCGCCACATGGTCTACGCCGCCGGCCGTCAGGACCATACCGTTCCGGCCGATTC
GATGATCGAGCACATCGTCGAGCTGGTCGAGAAGAAGGCCGAGATCCTCCGTGCCGAGGAAGCGGCCGCCAAGGCCGAGG
CCGAAGCGGCGCTGGCCCCCGCGCAATAA

Upstream 100 bases:

>100_bases
AACTTGGCGGTTTTTGGCAGGAAGCACCCACGGGAAGGCGGCGCACCGTCGGGCCGTCGGATAGAACCGTGTTACAATTG
CCTCATCCGGAGGGCAGATC

Downstream 100 bases:

>100_bases
GGGTCCCTTCAACGCCCCCGCGGGCCGCCGAGCCGCGGCCGCCGGGGCACCTGTTCCCATCAACCAGGAATTTTGTCTTC
GTGAGCAGCTTGCAACCCGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSSYRPYQSIERRKSRQIHVGKVPVGGDAPISVQTMTNTLTTDAEATIAQIRRAELAGVDIVRVSCPDEESTAALAEIVR
EVNVPIVADIHFHYKRAIEAAQAGAACLRINPGNIGSAERVREVVKAAREHGCSIRIGVNAGSLEKHLLEKYGEPNPDAL
VESALEHAKILQDHDFHEFKISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGSKRAGTVKSSIGLGNLLWAGVGDTMR
VSLSADPEEEVLVGWDILKSLGLRHRGVKIISCPSCARQGFNVIQTVQTLEERLAHIQTPLTLSIIGCVVNGPGEALMTD
IGVTGGGSGRHMVYAAGRQDHTVPADSMIEHIVELVEKKAEILRAEEAAAKAEAEAALAPAQ

Sequences:

>Translated_382_residues
MSSYRPYQSIERRKSRQIHVGKVPVGGDAPISVQTMTNTLTTDAEATIAQIRRAELAGVDIVRVSCPDEESTAALAEIVR
EVNVPIVADIHFHYKRAIEAAQAGAACLRINPGNIGSAERVREVVKAAREHGCSIRIGVNAGSLEKHLLEKYGEPNPDAL
VESALEHAKILQDHDFHEFKISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGSKRAGTVKSSIGLGNLLWAGVGDTMR
VSLSADPEEEVLVGWDILKSLGLRHRGVKIISCPSCARQGFNVIQTVQTLEERLAHIQTPLTLSIIGCVVNGPGEALMTD
IGVTGGGSGRHMVYAAGRQDHTVPADSMIEHIVELVEKKAEILRAEEAAAKAEAEAALAPAQ
>Mature_381_residues
SSYRPYQSIERRKSRQIHVGKVPVGGDAPISVQTMTNTLTTDAEATIAQIRRAELAGVDIVRVSCPDEESTAALAEIVRE
VNVPIVADIHFHYKRAIEAAQAGAACLRINPGNIGSAERVREVVKAAREHGCSIRIGVNAGSLEKHLLEKYGEPNPDALV
ESALEHAKILQDHDFHEFKISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGSKRAGTVKSSIGLGNLLWAGVGDTMRV
SLSADPEEEVLVGWDILKSLGLRHRGVKIISCPSCARQGFNVIQTVQTLEERLAHIQTPLTLSIIGCVVNGPGEALMTDI
GVTGGGSGRHMVYAAGRQDHTVPADSMIEHIVELVEKKAEILRAEEAAAKAEAEAALAPAQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=354, Percent_Identity=57.3446327683616, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_GLUDA (A9HJ48)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001602158.1
- RefSeq:   YP_002274550.1
- ProteinModelPortal:   A9HJ48
- GeneID:   5790167
- GeneID:   6973527
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_0135
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR016425
- InterPro:   IPR004588
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51366 RibP_bind_barrel

EC number: =1.17.7.1

Molecular weight: Translated: 40901; Mature: 40770

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYRPYQSIERRKSRQIHVGKVPVGGDAPISVQTMTNTLTTDAEATIAQIRRAELAGVD
CCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCE
IVRVSCPDEESTAALAEIVREVNVPIVADIHFHYKRAIEAAQAGAACLRINPGNIGSAER
EEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
VREVVKAAREHGCSIRIGVNAGSLEKHLLEKYGEPNPDALVESALEHAKILQDHDFHEFK
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
ISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGSKRAGTVKSSIGLGNLLWAGVGDTMR
EEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCHHHEECCCCEEE
VSLSADPEEEVLVGWDILKSLGLRHRGVKIISCPSCARQGFNVIQTVQTLEERLAHIQTP
EEECCCCCCCEEEHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
LTLSIIGCVVNGPGEALMTDIGVTGGGSGRHMVYAAGRQDHTVPADSMIEHIVELVEKKA
CEEEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EILRAEEAAAKAEAEAALAPAQ
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSYRPYQSIERRKSRQIHVGKVPVGGDAPISVQTMTNTLTTDAEATIAQIRRAELAGVD
CCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCE
IVRVSCPDEESTAALAEIVREVNVPIVADIHFHYKRAIEAAQAGAACLRINPGNIGSAER
EEEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCHHH
VREVVKAAREHGCSIRIGVNAGSLEKHLLEKYGEPNPDALVESALEHAKILQDHDFHEFK
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
ISVKASDVFLAVAAYQQLAEVCDHPLHIGITEAGSKRAGTVKSSIGLGNLLWAGVGDTMR
EEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCCCHHHEECCCCEEE
VSLSADPEEEVLVGWDILKSLGLRHRGVKIISCPSCARQGFNVIQTVQTLEERLAHIQTP
EEECCCCCCCEEEHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
LTLSIIGCVVNGPGEALMTDIGVTGGGSGRHMVYAAGRQDHTVPADSMIEHIVELVEKKA
CEEEEEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EILRAEEAAAKAEAEAALAPAQ
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA