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The map label for this gene is cspE [H]

Identifier: 162147665

GI number: 162147665

Start: 1915270

End: 1915950

Strand: Reverse

Name: cspE [H]

Synonym: GDI_1881

Alternate gene names: 162147665

Gene position: 1915950-1915270 (Counterclockwise)

Preceding gene: 162147666

Following gene: 162147660

Centisome position: 48.58

GC content: 70.04

Gene sequence:

>681_bases
TTGAGAAGTAACAGGACCGACCGCAGCCCCCGTTCCCCGCGCCGCGGCGGATTTGACGACGATTTCATGTCGTCGCCCTC
GTTTGGTGACCGCAGCGGCGGCGGTGCCCCGCGCCGGCCGATGGGCGGTGGCGCTCCGTCCCAGGTCATCGCCTCGGGCC
CGGAAATCAGTGCCGCCGTCAAGTGGTTCAACAGCGAGAAAGGCTTCGGCTTCGTCGAGCTGTCCGATGGCTCGGGAGAC
GTCTTCCTCCACGCCAACGCGCTGGCGACCAGCGGACATGATTCCGTCTCGCCCGGAACGACGCTGGTCGTCCGCATCGG
CCAGGGCCCCAAGGGCCGTCAGGTCGCGTCCGTGGTCTCGGTGGACGACAGCACCGCGCAGCCGGAACGTCCGCGCGGCT
TCGGCGCCCCGCGTGCCGCCGCCGGCGGCTTTGGCGGCCGTCCGTCCCGCCCGGCCCCCGACCTGTCGCAGGCGCAGGAA
ATGCGCGGCGTGGTCAAGTGGTACAACGCCACGAAGGGCTTCGGCTTCATCACGCCGGAATCCGGCGGCAAGGACATCTT
CGTCCACGCCTCGGCGCTGGAGCGTTCGGGCCTGACCGGCCTGACCGAAGGCCAGACGGCGAACGTGCAGGTCGTCCAGG
GCCAGAAGGGTCCCGAGGCCGCGTCGATCAGCGTCGACTGA

Upstream 100 bases:

>100_bases
CGGTTGGATTACGGCTTTGTAATTCCTTGATCCAGCAAGATTTCTTCATCCGGTGGCTCCTGGGACAGGACCCGCCAACT
AGACCTCGGGATTACGACCG

Downstream 100 bases:

>100_bases
CCTCCCGGTTTCCCACCGGAACACCAGACAGGGCCGCCTTCGGGCGGCCCTGTTTCGTTTGCGGCTATTCCCTGCCCTTG
TTGCGCAGCAGCCGCGCCTT

Product: cold shock-like protein cspE

Products: NA

Alternate protein names: CSP-E [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MRSNRTDRSPRSPRRGGFDDDFMSSPSFGDRSGGGAPRRPMGGGAPSQVIASGPEISAAVKWFNSEKGFGFVELSDGSGD
VFLHANALATSGHDSVSPGTTLVVRIGQGPKGRQVASVVSVDDSTAQPERPRGFGAPRAAAGGFGGRPSRPAPDLSQAQE
MRGVVKWYNATKGFGFITPESGGKDIFVHASALERSGLTGLTEGQTANVQVVQGQKGPEAASISVD

Sequences:

>Translated_226_residues
MRSNRTDRSPRSPRRGGFDDDFMSSPSFGDRSGGGAPRRPMGGGAPSQVIASGPEISAAVKWFNSEKGFGFVELSDGSGD
VFLHANALATSGHDSVSPGTTLVVRIGQGPKGRQVASVVSVDDSTAQPERPRGFGAPRAAAGGFGGRPSRPAPDLSQAQE
MRGVVKWYNATKGFGFITPESGGKDIFVHASALERSGLTGLTEGQTANVQVVQGQKGPEAASISVD
>Mature_226_residues
MRSNRTDRSPRSPRRGGFDDDFMSSPSFGDRSGGGAPRRPMGGGAPSQVIASGPEISAAVKWFNSEKGFGFVELSDGSGD
VFLHANALATSGHDSVSPGTTLVVRIGQGPKGRQVASVVSVDDSTAQPERPRGFGAPRAAAGGFGGRPSRPAPDLSQAQE
MRGVVKWYNATKGFGFITPESGGKDIFVHASALERSGLTGLTEGQTANVQVVQGQKGPEAASISVD

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=51.5625, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=50.7692307692308, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=48.4848484848485, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=49.2307692307692, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787839, Length=69, Percent_Identity=46.3768115942029, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787834, Length=67, Percent_Identity=46.2686567164179, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=50, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 23330; Mature: 23330

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNRTDRSPRSPRRGGFDDDFMSSPSFGDRSGGGAPRRPMGGGAPSQVIASGPEISAAV
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEE
KWFNSEKGFGFVELSDGSGDVFLHANALATSGHDSVSPGTTLVVRIGQGPKGRQVASVVS
EEECCCCCCEEEEEECCCCCEEEEECEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEE
VDDSTAQPERPRGFGAPRAAAGGFGGRPSRPAPDLSQAQEMRGVVKWYNATKGFGFITPE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCC
SGGKDIFVHASALERSGLTGLTEGQTANVQVVQGQKGPEAASISVD
CCCCEEEEEHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECC
>Mature Secondary Structure
MRSNRTDRSPRSPRRGGFDDDFMSSPSFGDRSGGGAPRRPMGGGAPSQVIASGPEISAAV
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCEEEE
KWFNSEKGFGFVELSDGSGDVFLHANALATSGHDSVSPGTTLVVRIGQGPKGRQVASVVS
EEECCCCCCEEEEEECCCCCEEEEECEEEECCCCCCCCCCEEEEEECCCCCCCEEEEEEE
VDDSTAQPERPRGFGAPRAAAGGFGGRPSRPAPDLSQAQEMRGVVKWYNATKGFGFITPE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCC
SGGKDIFVHASALERSGLTGLTEGQTANVQVVQGQKGPEAASISVD
CCCCEEEEEHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]