Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 162147581

GI number: 162147581

Start: 1828415

End: 1829683

Strand: Direct

Name: dacA [H]

Synonym: GDI_1797

Alternate gene names: 162147581

Gene position: 1828415-1829683 (Clockwise)

Preceding gene: 162147580

Following gene: 162147582

Centisome position: 46.36

GC content: 68.64

Gene sequence:

>1269_bases
ATGAACGTGCAGACCCGAAGGTTCCTCATGGCTGGGACGGCCTCGCTGGCCGCCACCGGTTCTCTTGCGGTTGCGGCGCC
GCGCCGGCACCACGCGCATGGCGCGCCGTCCCCCCAGCCCGCCCCGACCGGCGGAGCGAATGCGGAGGACGGCACCGATG
CCGCCGACGCGGCGGCCCCCGCCGGGCCGCCGGCCGCCACCCCCATCGGTCCGCTGGACACCGTGGCGCGCTGGGCCTGC
ATCCTGGACGTCACGACCGGATCGGTCCTGCTGGAAAAGGCGGCGGACGAGCGGATGCCGCCGTCCTCGCTGACCAAGAT
GATGACGGCCTATATCGTCTTCGGCATGCTGAAATCCGGCCGACTGAAGCTGGACCAGATGCTGCCGGTCAGCGAGAAGG
CGTGGCGTATGCAGGGGTCCAAGATGTTCGTGCCCCTGGGGCAGAGCATCGCGGTCCAGGACCTGATCCAGGGCATGGTC
ATCCAGTCGGGCAACGACGCGTGCATCGTGCTGGCCGAAGGCGTGGCGGGGTCCGAGGAACAGTTCGTCGCCCAGATGAA
CGACATGGCGGCGCGTATCGGCCTGTCCAATTCGCATTTCATGAACGCCACCGGCTGGCCGGCCGACAATCACTACATGT
CGGCGCGCGACGTCGCGCAACTGGCGGTACGGCTGATCCGCGACTTCCCGGAATATTATCACTTCTTTTCCGAGAAGGAT
TACCGGTTCAACAAGATCTCGCAGGGCAACCGCAACGTGCTGGTGGACAAGGGGCTGGCCGACGGGCTGAAGACCGGCCA
CACCGATGCCGGCGGGTTCGGGCTGTGCGCCAGCGCCGACCGGGGCGGCAACCGGGTGGTCCTGTCGCTGAACGGCATGC
CGTCCAGCAACGCCCGCGCGCATGAAGGCGAGCGCCTGCTGGAATGGTCGTTCGCGAATTTCGAGAACGCGACCCTGTTC
CGCAAGGGCGACATCGTCGAACAGGCATCGGTGTGGCTGGGCACGCAGCCCACCGTGCCCCTGGTCGCCACGCGGGATAT
CGTCATGACCCTGCCGCATGGCTGGCGGTCGCGGGCGCATATCGGGGTGGATTACCAGGCCCCGGTGGCCGCCCCCGTGG
CGGCAGGGCAGGTGCTGGGCGACCTGGTGATCAGCAATCCGGGCCTGGCCGATATCCGCATGCCCCTGGTGGCGGGGCAG
GGCGTGCCGCGCCTGGGCCTGTTCGCCCGGGCGTCGGCGGTGCTGGGCCAGAAACTGGGCCGGCACTGA

Upstream 100 bases:

>100_bases
TCCATGTCGGGCCGTTCATGAACGCGACCGACGCCGACCGCGCACTGGACCAGCTTATGGGCGCCGGAGTAACCGGTGCC
CGGATCGTCGTCGAATAGGG

Downstream 100 bases:

>100_bases
GTTCCGTGGCGGGCCTGTTCATCACCTTCGAAGGGGGCGAGGGCGCGGGGAAATCTACCCAGGCGCAGCGCCTGGAGGCG
CATCTGCGCGCCGCCGGCCG

Product: penicillin-binding protein 5

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNVQTRRFLMAGTASLAATGSLAVAAPRRHHAHGAPSPQPAPTGGANAEDGTDAADAAAPAGPPAATPIGPLDTVARWAC
ILDVTTGSVLLEKAADERMPPSSLTKMMTAYIVFGMLKSGRLKLDQMLPVSEKAWRMQGSKMFVPLGQSIAVQDLIQGMV
IQSGNDACIVLAEGVAGSEEQFVAQMNDMAARIGLSNSHFMNATGWPADNHYMSARDVAQLAVRLIRDFPEYYHFFSEKD
YRFNKISQGNRNVLVDKGLADGLKTGHTDAGGFGLCASADRGGNRVVLSLNGMPSSNARAHEGERLLEWSFANFENATLF
RKGDIVEQASVWLGTQPTVPLVATRDIVMTLPHGWRSRAHIGVDYQAPVAAPVAAGQVLGDLVISNPGLADIRMPLVAGQ
GVPRLGLFARASAVLGQKLGRH

Sequences:

>Translated_422_residues
MNVQTRRFLMAGTASLAATGSLAVAAPRRHHAHGAPSPQPAPTGGANAEDGTDAADAAAPAGPPAATPIGPLDTVARWAC
ILDVTTGSVLLEKAADERMPPSSLTKMMTAYIVFGMLKSGRLKLDQMLPVSEKAWRMQGSKMFVPLGQSIAVQDLIQGMV
IQSGNDACIVLAEGVAGSEEQFVAQMNDMAARIGLSNSHFMNATGWPADNHYMSARDVAQLAVRLIRDFPEYYHFFSEKD
YRFNKISQGNRNVLVDKGLADGLKTGHTDAGGFGLCASADRGGNRVVLSLNGMPSSNARAHEGERLLEWSFANFENATLF
RKGDIVEQASVWLGTQPTVPLVATRDIVMTLPHGWRSRAHIGVDYQAPVAAPVAAGQVLGDLVISNPGLADIRMPLVAGQ
GVPRLGLFARASAVLGQKLGRH
>Mature_422_residues
MNVQTRRFLMAGTASLAATGSLAVAAPRRHHAHGAPSPQPAPTGGANAEDGTDAADAAAPAGPPAATPIGPLDTVARWAC
ILDVTTGSVLLEKAADERMPPSSLTKMMTAYIVFGMLKSGRLKLDQMLPVSEKAWRMQGSKMFVPLGQSIAVQDLIQGMV
IQSGNDACIVLAEGVAGSEEQFVAQMNDMAARIGLSNSHFMNATGWPADNHYMSARDVAQLAVRLIRDFPEYYHFFSEKD
YRFNKISQGNRNVLVDKGLADGLKTGHTDAGGFGLCASADRGGNRVVLSLNGMPSSNARAHEGERLLEWSFANFENATLF
RKGDIVEQASVWLGTQPTVPLVATRDIVMTLPHGWRSRAHIGVDYQAPVAAPVAAGQVLGDLVISNPGLADIRMPLVAGQ
GVPRLGLFARASAVLGQKLGRH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=344, Percent_Identity=38.3720930232558, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786851, Length=340, Percent_Identity=36.4705882352941, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082022, Length=350, Percent_Identity=34.2857142857143, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI87082054, Length=208, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44782; Mature: 44782

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVQTRRFLMAGTASLAATGSLAVAAPRRHHAHGAPSPQPAPTGGANAEDGTDAADAAAP
CCCCCCEEEEECCHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
AGPPAATPIGPLDTVARWACILDVTTGSVLLEKAADERMPPSSLTKMMTAYIVFGMLKSG
CCCCCCCCCCCHHHHHHEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
RLKLDQMLPVSEKAWRMQGSKMFVPLGQSIAVQDLIQGMVIQSGNDACIVLAEGVAGSEE
CEEHHHCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCHH
QFVAQMNDMAARIGLSNSHFMNATGWPADNHYMSARDVAQLAVRLIRDFPEYYHFFSEKD
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCC
YRFNKISQGNRNVLVDKGLADGLKTGHTDAGGFGLCASADRGGNRVVLSLNGMPSSNARA
CCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCC
HEGERLLEWSFANFENATLFRKGDIVEQASVWLGTQPTVPLVATRDIVMTLPHGWRSRAH
CCCCEEEEEECCCCCCCEEEECCCCHHHHHEEECCCCCCCEEEECCEEEECCCCCCCCCE
IGVDYQAPVAAPVAAGQVLGDLVISNPGLADIRMPLVAGQGVPRLGLFARASAVLGQKLG
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHC
RH
CC
>Mature Secondary Structure
MNVQTRRFLMAGTASLAATGSLAVAAPRRHHAHGAPSPQPAPTGGANAEDGTDAADAAAP
CCCCCCEEEEECCHHHHCCCCEEEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
AGPPAATPIGPLDTVARWACILDVTTGSVLLEKAADERMPPSSLTKMMTAYIVFGMLKSG
CCCCCCCCCCCHHHHHHEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
RLKLDQMLPVSEKAWRMQGSKMFVPLGQSIAVQDLIQGMVIQSGNDACIVLAEGVAGSEE
CEEHHHCCCCCHHHHHCCCCEEEEECCCHHHHHHHHHHHEEECCCCEEEEEECCCCCCHH
QFVAQMNDMAARIGLSNSHFMNATGWPADNHYMSARDVAQLAVRLIRDFPEYYHFFSEKD
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCC
YRFNKISQGNRNVLVDKGLADGLKTGHTDAGGFGLCASADRGGNRVVLSLNGMPSSNARA
CCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCC
HEGERLLEWSFANFENATLFRKGDIVEQASVWLGTQPTVPLVATRDIVMTLPHGWRSRAH
CCCCEEEEEECCCCCCCEEEECCCCHHHHHEEECCCCCCCEEEECCEEEECCCCCCCCCE
IGVDYQAPVAAPVAAGQVLGDLVISNPGLADIRMPLVAGQGVPRLGLFARASAVLGQKLG
ECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHEEECCCCCCHHHHHHHHHHHHHHHC
RH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]