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The map label for this gene is dnaN [H]

Identifier: 162147556

GI number: 162147556

Start: 1799776

End: 1800900

Strand: Direct

Name: dnaN [H]

Synonym: GDI_1772

Alternate gene names: 162147556

Gene position: 1799776-1800900 (Clockwise)

Preceding gene: 162147555

Following gene: 162147557

Centisome position: 45.63

GC content: 65.51

Gene sequence:

>1125_bases
ATGAAGCTGAAGGCTGACCGCGCGACATTGCTGAAGGCGCTGGCCCACATCCAGAGCGTCGCCGAGAAGCGGAATACCAT
TCCGATCCTGGCCAATGTCCTGATCAATGTCGCGGATGGACGGCTGACGCTGACCGCCACCGACATGGAAATCGCGGTGG
TCGAGGAAATCGGGGCCGAGACCAGCCGCAACGGCGCGGTGACCGCCCCGGCCGCCGTGCTGTACGAGATCGCGCGCAAG
CTGCCCGACGGGGTCGCGATCGAACTGGACCATACCGGCGGCGACGCGCCGCTGGCATTGCGGGCCGGCCGCTACGCCAC
CAGCCTGAACGTGCTGGATGTCGATGACTTCCCTTCGATGATGGCCGGGACGCTGCCGTACCATTTCACGATGCCGGCCG
CGGTGCTGCGCGGGCTGATCGACCGGACGCGCTTCGCCATCTCGACCGAGGAGACGCGCTATTACCTGAACGGCATCTAC
CTGCATGTCGCGGCAGGTGATGAAGGCAGCATGCTGCGCGCGGTGGCCACCGACGGGCACCGCCTGGCCCGTGTCGAGAC
CGAGGTCCCGCCGGGCGCCGAGGAGATGCCCGGTGTCATCGTGCCCCGCAAGACAGTCGCGGAACTGCGCAAGCTGCTGG
ACGAGGGGACCGAGGACGTGGCGGTGGGCCTGTCCGATACCCGCATCCAGTTCACGGTCGGCACCGTGACCCTGACCTCG
AAGCTGATCGACGGGACGTTCCCGGAATATGATCGCGTCATCCCGCGCGGAAACGACAAGATCCTGCGCGTCGGCAAGCG
TGATTTTTCCGATGCGGTCGCGCGTGTCGCCGCGATCAGCCAGGAGCGGTCGCGCCCCGTCAAGCTGTCGATCGGCCACA
ACCTGCTGACCCTGTCGGCGGTCAGCCCCGACCAGGGCACCGCGAAGGAAGAACTGGACGACAATTACGTCACCTACGAT
TCCACGCCGCTGGAAATCGGCTTCCAGGCCCGCTACCTGAACGACATCACGGACCAGATCGACAAGGATGTCGAATTCGC
GTTTTCGGACAGTTCCGCGCCGACGATCGTCCGTGACGTGGCCAGCCCTTCGGCGTTGTACGTCCTGATGCCGATGCGGG
TCTGA

Upstream 100 bases:

>100_bases
GCGACTGCCGGACAACGATCCGGGTGATCCGTTGCCGGAACCCATTCCCCCGTCAGGCCGGCCGCAGCGGGTTGGCCTCG
GGCAAGGAGAGTCTCTACCC

Downstream 100 bases:

>100_bases
CCAGGACCCGACCCGCTGCGTCTTCGATCCGCTGCATCATGGCGCGTCTCGACCGGCTGGCGCTGACGGATTTCAGGAAC
TACCGGCACCTGGCCTGGCG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKLKADRATLLKALAHIQSVAEKRNTIPILANVLINVADGRLTLTATDMEIAVVEEIGAETSRNGAVTAPAAVLYEIARK
LPDGVAIELDHTGGDAPLALRAGRYATSLNVLDVDDFPSMMAGTLPYHFTMPAAVLRGLIDRTRFAISTEETRYYLNGIY
LHVAAGDEGSMLRAVATDGHRLARVETEVPPGAEEMPGVIVPRKTVAELRKLLDEGTEDVAVGLSDTRIQFTVGTVTLTS
KLIDGTFPEYDRVIPRGNDKILRVGKRDFSDAVARVAAISQERSRPVKLSIGHNLLTLSAVSPDQGTAKEELDDNYVTYD
STPLEIGFQARYLNDITDQIDKDVEFAFSDSSAPTIVRDVASPSALYVLMPMRV

Sequences:

>Translated_374_residues
MKLKADRATLLKALAHIQSVAEKRNTIPILANVLINVADGRLTLTATDMEIAVVEEIGAETSRNGAVTAPAAVLYEIARK
LPDGVAIELDHTGGDAPLALRAGRYATSLNVLDVDDFPSMMAGTLPYHFTMPAAVLRGLIDRTRFAISTEETRYYLNGIY
LHVAAGDEGSMLRAVATDGHRLARVETEVPPGAEEMPGVIVPRKTVAELRKLLDEGTEDVAVGLSDTRIQFTVGTVTLTS
KLIDGTFPEYDRVIPRGNDKILRVGKRDFSDAVARVAAISQERSRPVKLSIGHNLLTLSAVSPDQGTAKEELDDNYVTYD
STPLEIGFQARYLNDITDQIDKDVEFAFSDSSAPTIVRDVASPSALYVLMPMRV
>Mature_374_residues
MKLKADRATLLKALAHIQSVAEKRNTIPILANVLINVADGRLTLTATDMEIAVVEEIGAETSRNGAVTAPAAVLYEIARK
LPDGVAIELDHTGGDAPLALRAGRYATSLNVLDVDDFPSMMAGTLPYHFTMPAAVLRGLIDRTRFAISTEETRYYLNGIY
LHVAAGDEGSMLRAVATDGHRLARVETEVPPGAEEMPGVIVPRKTVAELRKLLDEGTEDVAVGLSDTRIQFTVGTVTLTS
KLIDGTFPEYDRVIPRGNDKILRVGKRDFSDAVARVAAISQERSRPVKLSIGHNLLTLSAVSPDQGTAKEELDDNYVTYD
STPLEIGFQARYLNDITDQIDKDVEFAFSDSSAPTIVRDVASPSALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=378, Percent_Identity=39.1534391534392, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40563; Mature: 40563

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKADRATLLKALAHIQSVAEKRNTIPILANVLINVADGRLTLTATDMEIAVVEEIGAE
CCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHHEEECCCEEEEEECCCEEEEHHHHCCC
TSRNGAVTAPAAVLYEIARKLPDGVAIELDHTGGDAPLALRAGRYATSLNVLDVDDFPSM
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEECCCCHHH
MAGTLPYHFTMPAAVLRGLIDRTRFAISTEETRYYLNGIYLHVAAGDEGSMLRAVATDGH
HHCCCCCEEECHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCC
RLARVETEVPPGAEEMPGVIVPRKTVAELRKLLDEGTEDVAVGLSDTRIQFTVGTVTLTS
EEEEEECCCCCCHHHCCCEEECHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEEEEH
KLIDGTFPEYDRVIPRGNDKILRVGKRDFSDAVARVAAISQERSRPVKLSIGHNLLTLSA
HHHCCCCCCHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
VSPDQGTAKEELDDNYVTYDSTPLEIGFQARYLNDITDQIDKDVEFAFSDSSAPTIVRDV
CCCCCCCCHHHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHC
ASPSALYVLMPMRV
CCCCEEEEEEECCC
>Mature Secondary Structure
MKLKADRATLLKALAHIQSVAEKRNTIPILANVLINVADGRLTLTATDMEIAVVEEIGAE
CCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEHHHEEECCCEEEEEECCCEEEEHHHHCCC
TSRNGAVTAPAAVLYEIARKLPDGVAIELDHTGGDAPLALRAGRYATSLNVLDVDDFPSM
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCEEEEEEEEECCCCHHH
MAGTLPYHFTMPAAVLRGLIDRTRFAISTEETRYYLNGIYLHVAAGDEGSMLRAVATDGH
HHCCCCCEEECHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEEECCCCCEEEEEECCCC
RLARVETEVPPGAEEMPGVIVPRKTVAELRKLLDEGTEDVAVGLSDTRIQFTVGTVTLTS
EEEEEECCCCCCHHHCCCEEECHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEEEEEH
KLIDGTFPEYDRVIPRGNDKILRVGKRDFSDAVARVAAISQERSRPVKLSIGHNLLTLSA
HHHCCCCCCHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
VSPDQGTAKEELDDNYVTYDSTPLEIGFQARYLNDITDQIDKDVEFAFSDSSAPTIVRDV
CCCCCCCCHHHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHC
ASPSALYVLMPMRV
CCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]