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The map label for this gene is ubiE [H]

Identifier: 162147552

GI number: 162147552

Start: 1795764

End: 1796561

Strand: Reverse

Name: ubiE [H]

Synonym: GDI_1768

Alternate gene names: 162147552

Gene position: 1796561-1795764 (Counterclockwise)

Preceding gene: 162147560

Following gene: 162147551

Centisome position: 45.55

GC content: 69.3

Gene sequence:

>798_bases
ATGACCGACAATCCCACCCCCTCTCCGTTCGCGCCCCATCCCGCCGGCCCCGATGCCACTGGAACGGGGACGACCGATTT
CGGCTTCCGCGACGTTCCCGTGGCGGACAAGAAGCCGATGGTCCGCGCCGTGTTCGACAGCGTCGCCTCGCGCTACGACA
TCATGAACGACGTGATGTCGCTGGGCATCCATCGCGCCTGGAAGAAGATCTTCGTGACCGAGCTGGGCGCGCACCCCGAC
CTGACGCTGCTGGACCTGGCCGGCGGCACGGGCGACATCTCGTTCGGGTGGCTGCGCGCCGGCGGCGGGCAGGCGATCCT
GTCGGACATCAATGCCAGCATGCTGGCCGTGGGGCGCAGCCGGGCACTGGAACGCGGCCTGATCTCCAGCCTGCGATTCG
CCGTGATGGATGCCGAGGCGATTCCCCTGCCCGACCGCTCGGTCGACCGGGTGTCGATCGCCTTCGGCCTGCGCAACTGC
ACCGACAAGGACGCCGTGCTGCGCGAGGCCAGGCGCGTGCTGCGCCCGGGCGGCAAGTTCATGTGCCTGGAATTCTCGCG
CGTGCAGGTGGCGGCGCTGTCGCCGATCTATGACGCGTGGTCGTTCAAGGTCCTGCCGCGCATGGGGGCCGCCATCGCCG
GCGACCGCGACAGCTACCAGTACCTGGCCGAAAGCATCCGCATGTTCCCCGACCAGGAGACGCTGGCCGGCATGATGCGC
GCCGCGGGGCTGGAGCGCGTGTCGGTCCGCAACCTGTCGGGCGGGATCGCCGCCATCCATTCCGGCTGGCGGATCTGA

Upstream 100 bases:

>100_bases
GCGATCGTCTTCCCGTCGAGATGCAATCGCATCCCGCGCATCACTGTTTCGACTTCAGGGAGTTCCGGCATGAGCGGATC
TGAGGTTATATCCCTGGGAC

Downstream 100 bases:

>100_bases
ACGTGGCCGCCCCCCAGCCGGCCGCCCCTCACCCCGCCGGGGGGCGCACGGTCCTGCTGATCGTCTGCGGGGGGATCGCG
GCGTACAAGACCCTGGAACT

Product: ubiquinone/menaquinone biosynthesis methyltransferase ubi

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTDNPTPSPFAPHPAGPDATGTGTTDFGFRDVPVADKKPMVRAVFDSVASRYDIMNDVMSLGIHRAWKKIFVTELGAHPD
LTLLDLAGGTGDISFGWLRAGGGQAILSDINASMLAVGRSRALERGLISSLRFAVMDAEAIPLPDRSVDRVSIAFGLRNC
TDKDAVLREARRVLRPGGKFMCLEFSRVQVAALSPIYDAWSFKVLPRMGAAIAGDRDSYQYLAESIRMFPDQETLAGMMR
AAGLERVSVRNLSGGIAAIHSGWRI

Sequences:

>Translated_265_residues
MTDNPTPSPFAPHPAGPDATGTGTTDFGFRDVPVADKKPMVRAVFDSVASRYDIMNDVMSLGIHRAWKKIFVTELGAHPD
LTLLDLAGGTGDISFGWLRAGGGQAILSDINASMLAVGRSRALERGLISSLRFAVMDAEAIPLPDRSVDRVSIAFGLRNC
TDKDAVLREARRVLRPGGKFMCLEFSRVQVAALSPIYDAWSFKVLPRMGAAIAGDRDSYQYLAESIRMFPDQETLAGMMR
AAGLERVSVRNLSGGIAAIHSGWRI
>Mature_264_residues
TDNPTPSPFAPHPAGPDATGTGTTDFGFRDVPVADKKPMVRAVFDSVASRYDIMNDVMSLGIHRAWKKIFVTELGAHPDL
TLLDLAGGTGDISFGWLRAGGGQAILSDINASMLAVGRSRALERGLISSLRFAVMDAEAIPLPDRSVDRVSIAFGLRNCT
DKDAVLREARRVLRPGGKFMCLEFSRVQVAALSPIYDAWSFKVLPRMGAAIAGDRDSYQYLAESIRMFPDQETLAGMMRA
AGLERVSVRNLSGGIAAIHSGWRI

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=43.1159420289855, Blast_Score=232, Evalue=2e-61,
Organism=Escherichia coli, GI48994983, Length=244, Percent_Identity=50.8196721311475, Blast_Score=256, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17557039, Length=243, Percent_Identity=46.5020576131687, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323526, Length=272, Percent_Identity=43.0147058823529, Blast_Score=204, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24641702, Length=257, Percent_Identity=47.8599221789883, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28654; Mature: 28523

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNPTPSPFAPHPAGPDATGTGTTDFGFRDVPVADKKPMVRAVFDSVASRYDIMNDVMS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGIHRAWKKIFVTELGAHPDLTLLDLAGGTGDISFGWLRAGGGQAILSDINASMLAVGRS
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHH
RALERGLISSLRFAVMDAEAIPLPDRSVDRVSIAFGLRNCTDKDAVLREARRVLRPGGKF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
MCLEFSRVQVAALSPIYDAWSFKVLPRMGAAIAGDRDSYQYLAESIRMFPDQETLAGMMR
EEEEECCEEEEHHCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AAGLERVSVRNLSGGIAAIHSGWRI
HCCCHHHHHHCCCCCHHHHCCCCCC
>Mature Secondary Structure 
TDNPTPSPFAPHPAGPDATGTGTTDFGFRDVPVADKKPMVRAVFDSVASRYDIMNDVMS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGIHRAWKKIFVTELGAHPDLTLLDLAGGTGDISFGWLRAGGGQAILSDINASMLAVGRS
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHH
RALERGLISSLRFAVMDAEAIPLPDRSVDRVSIAFGLRNCTDKDAVLREARRVLRPGGKF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCE
MCLEFSRVQVAALSPIYDAWSFKVLPRMGAAIAGDRDSYQYLAESIRMFPDQETLAGMMR
EEEEECCEEEEHHCHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AAGLERVSVRNLSGGIAAIHSGWRI
HCCCHHHHHHCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA