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The map label for this gene is guaD [H]

Identifier: 162147516

GI number: 162147516

Start: 1752292

End: 1753641

Strand: Reverse

Name: guaD [H]

Synonym: GDI_1732

Alternate gene names: 162147516

Gene position: 1753641-1752292 (Counterclockwise)

Preceding gene: 162147517

Following gene: 162147514

Centisome position: 44.46

GC content: 66.96

Gene sequence:

>1350_bases
ATGACGGTCGATCTTCTTATCCGCGACGCCTATATCTACGTCGATCGCGATCATGAAATCGCGGACGGCTGGCTGGCCAT
CAAGGACCGGCGCGTTCATTCCGTCGGCGCTGAAGTCGCGACGGCGCCCCCCGCCCGGACCATCCTTTCCGCGCGGGTGC
AACTGGTCACGCCGGGGCTGATCAACGCGCATCACCACATGTACCAGAACCTGACGCGGGCTTATGCGCCGGTGACGAAC
GGCACGCTGTTCGAATGGCTGACGGGTCTCTATCCGCTATGGGCGGACCTGGACGCGGAATCGGTCTACCTGTCGACCTG
CGTCGCCATGGTCGAATTGCTGATGGGCGGCTGCACGACCTCGATGGACCATATGTATGTCCATCCCAAGCCCTTCCTGA
TCGACGCGCAGTTTCGCGCCGCGCGGGATATCGGCTTTCGTTTTTATGCCACGCGCGGGTCCATGACGCGGTCGGTGAAG
GATGGTGGCCTTCCGCCCGCATCCGTCGTGCAGGACGAGGACACCATCCTGGCGGACAGCGAACGGCTGGTCGCGGCGTT
CCATGACGCGTCGCCCGGCGCGATGGGCCGCGTGGCCCTGGCCCCGTGCTCGCTGTTCAGCGTGTCCGAGCGGATCATGA
CCGAATCGGCACGGCTGGCGGAAAAGCACGATCTGCGGCTGCACACCCATCTGGCGGAAGACCCCGACGAGGACCGGTAT
TGCGCCGATGTCTATGGGTGCACACCCACCGAATATTTCGAACGCGTGGGCTGGGCCACCCCCCGGTCGTGGGTGGCGCA
TTACATCTACCCCTCCAGCGACGAGATCGATCGCCTGGCCCATGCCGGGGTCTGCACGGCGCAGTGCCCGTGTTCCAACA
TGATGATCGGCGGCGGCGCGGCCGATGCGATGGGCATGCGCCGGCGCGGTATGCGGGTGGGGCTGGGCTGCGACGGCTCG
GCCTCCACCGACCACGGATCAATGTGGCTGGAAACCCGCATGGCGCTGCTGCTGGGTCGCTTCAGGAACGGCCCGCATTC
CATGTCGGCGCGTGATGCGCTGGACATGGCGACGCGCGGGGGCGCCGCCTGCCTGGGGTGGGAGGACGAGATCGGGCACC
TGCGGCCCGGCGCCTGCGCGGACCTGGTCATCTGGAAGATGTCGGATATCGCGCGGGCCGGGGCCTTGAGCGACCCGGTC
GAGGCCTGGCTGCGCTGCGCCCCCGCCGTCGCAGGGACGACAATCGTCAACGGCGCGATCCTGGTCGAGGACGGCGTGCC
GGTCATGTCCAACCTGGCCGAAATCCTGCGCGACCATGCGCGCGAGGCCCGTCGCATCCAGCGCCTGTAA

Upstream 100 bases:

>100_bases
ATACCGATTTGATGACGATCCGGACCTCGCACCTCCGGGACAGGGCTCATCGCGACAAGGCGCCCGCCTTGGCATCGGTA
TCTGCACGGAGGCCCCTATC

Downstream 100 bases:

>100_bases
CGCCCGGTGGGCGCGAGGTCATGCCCCGCGCCCACCGGACGGGCCGGACGTCAGGGCGCCGTCTTTTCCCTGGTGGCCAG
CAGGTCGCGGATTTCCTGCA

Product: guanine deaminase

Products: NA

Alternate protein names: Hydroxyatrazine hydrolase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTVDLLIRDAYIYVDRDHEIADGWLAIKDRRVHSVGAEVATAPPARTILSARVQLVTPGLINAHHHMYQNLTRAYAPVTN
GTLFEWLTGLYPLWADLDAESVYLSTCVAMVELLMGGCTTSMDHMYVHPKPFLIDAQFRAARDIGFRFYATRGSMTRSVK
DGGLPPASVVQDEDTILADSERLVAAFHDASPGAMGRVALAPCSLFSVSERIMTESARLAEKHDLRLHTHLAEDPDEDRY
CADVYGCTPTEYFERVGWATPRSWVAHYIYPSSDEIDRLAHAGVCTAQCPCSNMMIGGGAADAMGMRRRGMRVGLGCDGS
ASTDHGSMWLETRMALLLGRFRNGPHSMSARDALDMATRGGAACLGWEDEIGHLRPGACADLVIWKMSDIARAGALSDPV
EAWLRCAPAVAGTTIVNGAILVEDGVPVMSNLAEILRDHAREARRIQRL

Sequences:

>Translated_449_residues
MTVDLLIRDAYIYVDRDHEIADGWLAIKDRRVHSVGAEVATAPPARTILSARVQLVTPGLINAHHHMYQNLTRAYAPVTN
GTLFEWLTGLYPLWADLDAESVYLSTCVAMVELLMGGCTTSMDHMYVHPKPFLIDAQFRAARDIGFRFYATRGSMTRSVK
DGGLPPASVVQDEDTILADSERLVAAFHDASPGAMGRVALAPCSLFSVSERIMTESARLAEKHDLRLHTHLAEDPDEDRY
CADVYGCTPTEYFERVGWATPRSWVAHYIYPSSDEIDRLAHAGVCTAQCPCSNMMIGGGAADAMGMRRRGMRVGLGCDGS
ASTDHGSMWLETRMALLLGRFRNGPHSMSARDALDMATRGGAACLGWEDEIGHLRPGACADLVIWKMSDIARAGALSDPV
EAWLRCAPAVAGTTIVNGAILVEDGVPVMSNLAEILRDHAREARRIQRL
>Mature_448_residues
TVDLLIRDAYIYVDRDHEIADGWLAIKDRRVHSVGAEVATAPPARTILSARVQLVTPGLINAHHHMYQNLTRAYAPVTNG
TLFEWLTGLYPLWADLDAESVYLSTCVAMVELLMGGCTTSMDHMYVHPKPFLIDAQFRAARDIGFRFYATRGSMTRSVKD
GGLPPASVVQDEDTILADSERLVAAFHDASPGAMGRVALAPCSLFSVSERIMTESARLAEKHDLRLHTHLAEDPDEDRYC
ADVYGCTPTEYFERVGWATPRSWVAHYIYPSSDEIDRLAHAGVCTAQCPCSNMMIGGGAADAMGMRRRGMRVGLGCDGSA
STDHGSMWLETRMALLLGRFRNGPHSMSARDALDMATRGGAACLGWEDEIGHLRPGACADLVIWKMSDIARAGALSDPVE
AWLRCAPAVAGTTIVNGAILVEDGVPVMSNLAEILRDHAREARRIQRL

Specific function: Catalyzes the deamination reaction of hydroxyatrazine to N-isopropylammelide (dihydroxy-isopropyl-atrazine). The enzyme is also capable of catalyzing some dechlorinating reactions [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=345, Percent_Identity=24.6376811594203, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1789249, Length=378, Percent_Identity=28.8359788359788, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319963, Length=358, Percent_Identity=23.1843575418994, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24643849, Length=406, Percent_Identity=22.1674876847291, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.99.3 [H]

Molecular weight: Translated: 49115; Mature: 48983

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDLLIRDAYIYVDRDHEIADGWLAIKDRRVHSVGAEVATAPPARTILSARVQLVTPGL
CEEEEEEEEEEEEEECCCCCCCCEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHEECCCH
INAHHHMYQNLTRAYAPVTNGTLFEWLTGLYPLWADLDAESVYLSTCVAMVELLMGGCTT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
SMDHMYVHPKPFLIDAQFRAARDIGFRFYATRGSMTRSVKDGGLPPASVVQDEDTILADS
CCCCEEECCCCEEEECHHHHHHHCCEEEEEECCCCHHHHHCCCCCCHHHCCCCCCEEECC
ERLVAAFHDASPGAMGRVALAPCSLFSVSERIMTESARLAEKHDLRLHTHLAEDPDEDRY
CEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCE
CADVYGCTPTEYFERVGWATPRSWVAHYIYPSSDEIDRLAHAGVCTAQCPCSNMMIGGGA
EEHHCCCCHHHHHHHCCCCCCHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCCC
ADAMGMRRRGMRVGLGCDGSASTDHGSMWLETRMALLLGRFRNGPHSMSARDALDMATRG
HHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GAACLGWEDEIGHLRPGACADLVIWKMSDIARAGALSDPVEAWLRCAPAVAGTTIVNGAI
CCEEECCHHCCCCCCCCCHHHHEEEHHHHHHHHCCCCCHHHHHHHHCCHHHCCEEECCEE
LVEDGVPVMSNLAEILRDHAREARRIQRL
EEECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVDLLIRDAYIYVDRDHEIADGWLAIKDRRVHSVGAEVATAPPARTILSARVQLVTPGL
EEEEEEEEEEEEEECCCCCCCCEEEEECCHHHHHCCHHCCCCCHHHHHHHHHHEECCCH
INAHHHMYQNLTRAYAPVTNGTLFEWLTGLYPLWADLDAESVYLSTCVAMVELLMGGCTT
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
SMDHMYVHPKPFLIDAQFRAARDIGFRFYATRGSMTRSVKDGGLPPASVVQDEDTILADS
CCCCEEECCCCEEEECHHHHHHHCCEEEEEECCCCHHHHHCCCCCCHHHCCCCCCEEECC
ERLVAAFHDASPGAMGRVALAPCSLFSVSERIMTESARLAEKHDLRLHTHLAEDPDEDRY
CEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCE
CADVYGCTPTEYFERVGWATPRSWVAHYIYPSSDEIDRLAHAGVCTAQCPCSNMMIGGGA
EEHHCCCCHHHHHHHCCCCCCHHHHEEEECCCCHHHHHHHHCCCCCCCCCCCCEEECCCC
ADAMGMRRRGMRVGLGCDGSASTDHGSMWLETRMALLLGRFRNGPHSMSARDALDMATRG
HHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
GAACLGWEDEIGHLRPGACADLVIWKMSDIARAGALSDPVEAWLRCAPAVAGTTIVNGAI
CCEEECCHHCCCCCCCCCHHHHEEEHHHHHHHHCCCCCHHHHHHHHCCHHHCCEEECCEE
LVEDGVPVMSNLAEILRDHAREARRIQRL
EEECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9055410 [H]