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The map label for this gene is istA [C]

Identifier: 162147366

GI number: 162147366

Start: 1584094

End: 1585320

Strand: Reverse

Name: istA [C]

Synonym: GDI_1580

Alternate gene names: 162147366

Gene position: 1585320-1584094 (Counterclockwise)

Preceding gene: 162147367

Following gene: 162147365

Centisome position: 40.19

GC content: 65.28

Gene sequence:

>1227_bases
GTGTTGATCCACGAATTGAAACGACAGGGACTGGGTGTCAGCGCGATCGCGCGACAGACGGGCCTGGACCGCAAGACGGT
GCGCAGATATCTGGCCCGGGGCCTTGAAGCCCCACGCTACAGCCCCCGCGAGGAGAGCGAGCGCATTGCCGAACGCTTTC
GCACCTATTTGCTGGAAAGGCTGGAGACATATCCGGGTCTTTCCGCCCGCCGACTGCACCGCGAGATCAGGCCGATGGGA
TATGATGGGGCCTACTCGACGCTGACGGAATATCTGCGTCTCGTCAGACCGCCGGTGCTCAAGGTGTTCGAACGGCGCTT
CGAGACCGCTCCCGGCGCGCAGGCTCAGGTGGATTTCGCCGAATTTTCCGTGACCTTCGCGAGCGAACCGGAGGCGCCCC
GCAAGGTCTGGCTTTTCAGCATGGTACTGGGACACAGCCGCTGGCTCTGGGGCCGGTTCTGCCCGAACCAGACCCTGGAA
ACCGTCCTGCGCTGCCATATCGCCGCCTTCGAGGCGATGGGCGGAAGTTGCGCCGAGATCCTCTACGACCGCATGAAAAC
CGCGGTTCTCGGCGAAGACCCCGCGGGCATCGTGACCTACAATCCCTCGCTGGTCGCTCTGCTCGACCATTACGGCAGCG
CACCGCGCGCGTGCCAGCCGTATCGTGCCAAGACAAAGGGCAAGGTCGAGCGGCCGTTCCGCTATATCCGGCAGGATTTC
TTCCTCGGGCAGAGTTTTCGTGACCTCGATGAACTTAACGCCCGCTTCGACGACTGGCTGCGCACCATCGCCAATGCCAG
GTGTCATGCCACGACCGGCCGCATCGTCGCGGAACACTTCGCCGACGAGCGGCCTCATTTGCGAGCTCTGCCGGCCCATC
CGTATGACGCTGTCCTGACGGTCGAGCGCCGCGTCAGCCATGAAGGCATGGTCTCGGTCGGCGGCAATCTCTACAGCGTG
CCGGACACCGCCCGGCGCCGCATTCTCGAGATCCAGCACCATCCGACGGAAGTCCGGATCTTCGAGGAGGGTGTCATGAT
CGCCCGCCATCCGGTGCTGGACGGGCGCAACCAGCGCCGTGTCGATCCTGCGCACCGCAAGGCCGTGCCGGCGACGCGCC
AGATCAGGCCCCCGCCTGACCGGGCCGATGTGGCACGCCGGCCGCTCGCTTTCTATGAGGCGGTCGGTCGCAGGCTGGCC
ACCACGGGCGCGGGGGATCGGCCATGA

Upstream 100 bases:

>100_bases
ACCGTCCTGGCGGGGGCATGCCCCCGCCAGGACGGTCGTTCCCCAAATAACCTGCCTATGCCGACGGAAGGGCGAGCAGG
TGATTGGAGTCAGGGAGACT

Downstream 100 bases:

>100_bases
CCCCGGTGACCGAACGCATCCGCCAGAGCCTGGTCCACCTGCATATGGCGCGGGCCCTGGAAACCCTCGACCAGACCCTC
GGCCGCCTGGAGCGCGGCGA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLIHELKRQGLGVSAIARQTGLDRKTVRRYLARGLEAPRYSPREESERIAERFRTYLLERLETYPGLSARRLHREIRPMG
YDGAYSTLTEYLRLVRPPVLKVFERRFETAPGAQAQVDFAEFSVTFASEPEAPRKVWLFSMVLGHSRWLWGRFCPNQTLE
TVLRCHIAAFEAMGGSCAEILYDRMKTAVLGEDPAGIVTYNPSLVALLDHYGSAPRACQPYRAKTKGKVERPFRYIRQDF
FLGQSFRDLDELNARFDDWLRTIANARCHATTGRIVAEHFADERPHLRALPAHPYDAVLTVERRVSHEGMVSVGGNLYSV
PDTARRRILEIQHHPTEVRIFEEGVMIARHPVLDGRNQRRVDPAHRKAVPATRQIRPPPDRADVARRPLAFYEAVGRRLA
TTGAGDRP

Sequences:

>Translated_408_residues
MLIHELKRQGLGVSAIARQTGLDRKTVRRYLARGLEAPRYSPREESERIAERFRTYLLERLETYPGLSARRLHREIRPMG
YDGAYSTLTEYLRLVRPPVLKVFERRFETAPGAQAQVDFAEFSVTFASEPEAPRKVWLFSMVLGHSRWLWGRFCPNQTLE
TVLRCHIAAFEAMGGSCAEILYDRMKTAVLGEDPAGIVTYNPSLVALLDHYGSAPRACQPYRAKTKGKVERPFRYIRQDF
FLGQSFRDLDELNARFDDWLRTIANARCHATTGRIVAEHFADERPHLRALPAHPYDAVLTVERRVSHEGMVSVGGNLYSV
PDTARRRILEIQHHPTEVRIFEEGVMIARHPVLDGRNQRRVDPAHRKAVPATRQIRPPPDRADVARRPLAFYEAVGRRLA
TTGAGDRP
>Mature_408_residues
MLIHELKRQGLGVSAIARQTGLDRKTVRRYLARGLEAPRYSPREESERIAERFRTYLLERLETYPGLSARRLHREIRPMG
YDGAYSTLTEYLRLVRPPVLKVFERRFETAPGAQAQVDFAEFSVTFASEPEAPRKVWLFSMVLGHSRWLWGRFCPNQTLE
TVLRCHIAAFEAMGGSCAEILYDRMKTAVLGEDPAGIVTYNPSLVALLDHYGSAPRACQPYRAKTKGKVERPFRYIRQDF
FLGQSFRDLDELNARFDDWLRTIANARCHATTGRIVAEHFADERPHLRALPAHPYDAVLTVERRVSHEGMVSVGGNLYSV
PDTARRRILEIQHHPTEVRIFEEGVMIARHPVLDGRNQRRVDPAHRKAVPATRQIRPPPDRADVARRPLAFYEAVGRRLA
TTGAGDRP

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR017894
- InterPro:   IPR001584
- InterPro:   IPR006120
- InterPro:   IPR012337 [H]

Pfam domain/function: PF02796 HTH_7; PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 46658; Mature: 46658

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIHELKRQGLGVSAIARQTGLDRKTVRRYLARGLEAPRYSPREESERIAERFRTYLLER
CCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LETYPGLSARRLHREIRPMGYDGAYSTLTEYLRLVRPPVLKVFERRFETAPGAQAQVDFA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEHH
EFSVTFASEPEAPRKVWLFSMVLGHSRWLWGRFCPNQTLETVLRCHIAAFEAMGGSCAEI
HEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
LYDRMKTAVLGEDPAGIVTYNPSLVALLDHYGSAPRACQPYRAKTKGKVERPFRYIRQDF
HHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FLGQSFRDLDELNARFDDWLRTIANARCHATTGRIVAEHFADERPHLRALPAHPYDAVLT
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHH
VERRVSHEGMVSVGGNLYSVPDTARRRILEIQHHPTEVRIFEEGVMIARHPVLDGRNQRR
HHHHHCCCCCEEECCCEEECCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCC
VDPAHRKAVPATRQIRPPPDRADVARRPLAFYEAVGRRLATTGAGDRP
CCHHHHHCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLIHELKRQGLGVSAIARQTGLDRKTVRRYLARGLEAPRYSPREESERIAERFRTYLLER
CCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LETYPGLSARRLHREIRPMGYDGAYSTLTEYLRLVRPPVLKVFERRFETAPGAQAQVDFA
HHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEHH
EFSVTFASEPEAPRKVWLFSMVLGHSRWLWGRFCPNQTLETVLRCHIAAFEAMGGSCAEI
HEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHH
LYDRMKTAVLGEDPAGIVTYNPSLVALLDHYGSAPRACQPYRAKTKGKVERPFRYIRQDF
HHHHHHHHHCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
FLGQSFRDLDELNARFDDWLRTIANARCHATTGRIVAEHFADERPHLRALPAHPYDAVLT
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHH
VERRVSHEGMVSVGGNLYSVPDTARRRILEIQHHPTEVRIFEEGVMIARHPVLDGRNQRR
HHHHHCCCCCEEECCCEEECCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCCCCCCC
VDPAHRKAVPATRQIRPPPDRADVARRPLAFYEAVGRRLATTGAGDRP
CCHHHHHCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8382825 [H]