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The map label for this gene is istB [H]

Identifier: 162147365

GI number: 162147365

Start: 1583252

End: 1584097

Strand: Reverse

Name: istB [H]

Synonym: GDI_1579

Alternate gene names: 162147365

Gene position: 1584097-1583252 (Counterclockwise)

Preceding gene: 162147366

Following gene: 162147364

Centisome position: 40.16

GC content: 65.25

Gene sequence:

>846_bases
ATGACCCCGGTGACCGAACGCATCCGCCAGAGCCTGGTCCACCTGCATATGGCGCGGGCCCTGGAAACCCTCGACCAGAC
CCTCGGCCGCCTGGAGCGCGGCGACATCAGCGCGATCGAGGCCATCGACGAACTGCTGGCCGAGGAACTGAGCCTGCGAG
AGGGGCGTCGCATCGGCGTCGCCCTGCGAACCGCGCGCCTCATGCCGATCAAGACACTCGAAAGCTTCGACTTCTCTTTC
CAGCCCTCTCTCGAGCGAAACCGGATCATGGCGATCGCCCAACTCGAATTCATCGGTCGCGCGGAGGTCGTCCATTTCCT
TGGCCCGCCCGGGACAGGCAAGAGCCACCTCGCCACAGCCATCGGTGTCGCGGCGGTCAAGGCGGGACGCAGCGTCTATC
GCTGCACCCTCGCCGAACTGATCGAGGCGCTCGCTCGCGCCGAGCGCGAAGGCCGCCTCGCGGAGAAGATCCGTTTCTAC
GCCCGTGCCGCGCTGCTGATCGTCGACGAGATCGGATACCTGCCGATCACCAGCGGGGGCGCCAATCTGTTTTTCCAGTT
GGTCAACGCCCGATACGAGAAGGGATCGATGATCCTGACCTCCAACAGGGGCTTTGCCGAATGGGGCGACGTGTTCGGCG
ATCCGGTGGTGGCAACCGCGCTCCTCGATCGTCTCCTCCACCATGCCATCGTCGTGCAGATCGAAGGGGCCAGTTACCGG
TTGCGTCAACACGCGGACCTGGTGCCGGAACACACCCGGGCCCATGCCAATCTCACCCCGCCGCCGCTACCAAAACGCCG
TGGACGGCCACCCAGGAGAGAAACCGACGATCAAGGCCCTGGCTGA

Upstream 100 bases:

>100_bases
TCAGGCCCCCGCCTGACCGGGCCGATGTGGCACGCCGGCCGCTCGCTTTCTATGAGGCGGTCGGTCGCAGGCTGGCCACC
ACGGGCGCGGGGGATCGGCC

Downstream 100 bases:

>100_bases
TCCCCAGAAACCAGAAACTGGGGATTTTTCATCCAGCACTTCTGCGGACTTTTCATCCAGCATTTACAGTCATGGCCGGG
ATCCGGGCCCGGCATCCCGG

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTPVTERIRQSLVHLHMARALETLDQTLGRLERGDISAIEAIDELLAEELSLREGRRIGVALRTARLMPIKTLESFDFSF
QPSLERNRIMAIAQLEFIGRAEVVHFLGPPGTGKSHLATAIGVAAVKAGRSVYRCTLAELIEALARAEREGRLAEKIRFY
ARAALLIVDEIGYLPITSGGANLFFQLVNARYEKGSMILTSNRGFAEWGDVFGDPVVATALLDRLLHHAIVVQIEGASYR
LRQHADLVPEHTRAHANLTPPPLPKRRGRPPRRETDDQGPG

Sequences:

>Translated_281_residues
MTPVTERIRQSLVHLHMARALETLDQTLGRLERGDISAIEAIDELLAEELSLREGRRIGVALRTARLMPIKTLESFDFSF
QPSLERNRIMAIAQLEFIGRAEVVHFLGPPGTGKSHLATAIGVAAVKAGRSVYRCTLAELIEALARAEREGRLAEKIRFY
ARAALLIVDEIGYLPITSGGANLFFQLVNARYEKGSMILTSNRGFAEWGDVFGDPVVATALLDRLLHHAIVVQIEGASYR
LRQHADLVPEHTRAHANLTPPPLPKRRGRPPRRETDDQGPG
>Mature_280_residues
TPVTERIRQSLVHLHMARALETLDQTLGRLERGDISAIEAIDELLAEELSLREGRRIGVALRTARLMPIKTLESFDFSFQ
PSLERNRIMAIAQLEFIGRAEVVHFLGPPGTGKSHLATAIGVAAVKAGRSVYRCTLAELIEALARAEREGRLAEKIRFYA
RAALLIVDEIGYLPITSGGANLFFQLVNARYEKGSMILTSNRGFAEWGDVFGDPVVATALLDRLLHHAIVVQIEGASYRL
RQHADLVPEHTRAHANLTPPPLPKRRGRPPRRETDDQGPG

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 31130; Mature: 30999

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVTERIRQSLVHLHMARALETLDQTLGRLERGDISAIEAIDELLAEELSLREGRRIGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
ALRTARLMPIKTLESFDFSFQPSLERNRIMAIAQLEFIGRAEVVHFLGPPGTGKSHLATA
HHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHH
IGVAAVKAGRSVYRCTLAELIEALARAEREGRLAEKIRFYARAALLIVDEIGYLPITSGG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ANLFFQLVNARYEKGSMILTSNRGFAEWGDVFGDPVVATALLDRLLHHAIVVQIEGASYR
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCHH
LRQHADLVPEHTRAHANLTPPPLPKRRGRPPRRETDDQGPG
HHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPVTERIRQSLVHLHMARALETLDQTLGRLERGDISAIEAIDELLAEELSLREGRRIGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
ALRTARLMPIKTLESFDFSFQPSLERNRIMAIAQLEFIGRAEVVHFLGPPGTGKSHLATA
HHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCHHHEEEEECCCCCCHHHHHHH
IGVAAVKAGRSVYRCTLAELIEALARAEREGRLAEKIRFYARAALLIVDEIGYLPITSGG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ANLFFQLVNARYEKGSMILTSNRGFAEWGDVFGDPVVATALLDRLLHHAIVVQIEGASYR
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCHH
LRQHADLVPEHTRAHANLTPPPLPKRRGRPPRRETDDQGPG
HHHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]