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The map label for this gene is gidB

Identifier: 162147022

GI number: 162147022

Start: 1219172

End: 1219804

Strand: Reverse

Name: gidB

Synonym: GDI_1227

Alternate gene names: 162147022

Gene position: 1219804-1219172 (Counterclockwise)

Preceding gene: 162147023

Following gene: 162147021

Centisome position: 30.93

GC content: 66.82

Gene sequence:

>633_bases
ATGATGTTTCACGTGAAACCACAGGGGGGCAATGGCGTGCCGGATGCGCTGCGGGCGAAGCTGGACCGGTTCGCCGAAAT
CCTGACCCGCTGGAACAGCCGCATCAACCTGGTCTCGCCCCGGGACCTGGCACATCTGTGGGACCGGCATATCGCGGACT
GCCTGCAACTGGCGTCGCTGATCCCGCCCGGCGTGCGGCTGGCCGACATGGGGTCGGGCGGGGGCTTTCCCGGGCTGATC
ATCGCGATGGCCACCGACGCGGACGTGACCCTGATCGAATCCGACCAGCGCAAGGCCGCCTTCCTGCGCGAGGCCGCGCG
CGCGGTGGGCGCACGCGTCACCATCATCGCCCGCCGGATCGAGGACGCGGACATACCGCCCGTGCAGGTCGTCACCGCCC
GCGCCCTGGCCGCCCTGCCTCAATTGCTGGAATGGAGCAGCCATTTCCTGGCCCCGGATGGATTTTGTCTGTTCCTGAAG
GGCCGAAATGTCGAGGATGAGTTGACCGCTGCCGCCGCCGACTGGCACATGGCGGTATCCCGATTGCCAAGCCGCACCAA
CGCGGACGGCACCATTCTCAGACTGGGTGATATCAGGCGTGTCAGACACTCAGAACGCGCGCCGGGTCGCTGA

Upstream 100 bases:

>100_bases
TGGCCGCCGCCCGCCCCGCCAGCTTCAGCGCGGCGCAGCGGGTGCCGGGGATCACGCCCTCGGCACTGATGGCACTGCTG
TCGCATGTGCGGCAGGTGGC

Downstream 100 bases:

>100_bases
CGCCGCGCGCGTGCCTCGCATTCTTGCCCTGGCCAACCAGAAGGGCGGCGTCGGCAAGACGACCACGGCCATCAACCTCG
CCGCCGGCCTGGCGGCGCAG

Product: methyltransferase gidB (glucose-inhibited divisio protein B)

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MMFHVKPQGGNGVPDALRAKLDRFAEILTRWNSRINLVSPRDLAHLWDRHIADCLQLASLIPPGVRLADMGSGGGFPGLI
IAMATDADVTLIESDQRKAAFLREAARAVGARVTIIARRIEDADIPPVQVVTARALAALPQLLEWSSHFLAPDGFCLFLK
GRNVEDELTAAAADWHMAVSRLPSRTNADGTILRLGDIRRVRHSERAPGR

Sequences:

>Translated_210_residues
MMFHVKPQGGNGVPDALRAKLDRFAEILTRWNSRINLVSPRDLAHLWDRHIADCLQLASLIPPGVRLADMGSGGGFPGLI
IAMATDADVTLIESDQRKAAFLREAARAVGARVTIIARRIEDADIPPVQVVTARALAALPQLLEWSSHFLAPDGFCLFLK
GRNVEDELTAAAADWHMAVSRLPSRTNADGTILRLGDIRRVRHSERAPGR
>Mature_210_residues
MMFHVKPQGGNGVPDALRAKLDRFAEILTRWNSRINLVSPRDLAHLWDRHIADCLQLASLIPPGVRLADMGSGGGFPGLI
IAMATDADVTLIESDQRKAAFLREAARAVGARVTIIARRIEDADIPPVQVVTARALAALPQLLEWSSHFLAPDGFCLFLK
GRNVEDELTAAAADWHMAVSRLPSRTNADGTILRLGDIRRVRHSERAPGR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=172, Percent_Identity=28.4883720930233, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_GLUDA (A9HE10)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601483.1
- RefSeq:   YP_002276313.1
- ProteinModelPortal:   A9HE10
- SMR:   A9HE10
- GeneID:   5788760
- GeneID:   6975364
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1938
- HOGENOM:   HBG686577
- OMA:   GGQGWIV
- ProtClustDB:   CLSK936257
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22978; Mature: 22978

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFHVKPQGGNGVPDALRAKLDRFAEILTRWNSRINLVSPRDLAHLWDRHIADCLQLASL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHH
IPPGVRLADMGSGGGFPGLIIAMATDADVTLIESDQRKAAFLREAARAVGARVTIIARRI
CCCCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHEEHEEHH
EDADIPPVQVVTARALAALPQLLEWSSHFLAPDGFCLFLKGRNVEDELTAAAADWHMAVS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
RLPSRTNADGTILRLGDIRRVRHSERAPGR
HCCCCCCCCCEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMFHVKPQGGNGVPDALRAKLDRFAEILTRWNSRINLVSPRDLAHLWDRHIADCLQLASL
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHH
IPPGVRLADMGSGGGFPGLIIAMATDADVTLIESDQRKAAFLREAARAVGARVTIIARRI
CCCCCEEEECCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHEEHEEHH
EDADIPPVQVVTARALAALPQLLEWSSHFLAPDGFCLFLKGRNVEDELTAAAADWHMAVS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
RLPSRTNADGTILRLGDIRRVRHSERAPGR
HCCCCCCCCCEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA