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The map label for this gene is purU [H]

Identifier: 162146964

GI number: 162146964

Start: 1168872

End: 1169801

Strand: Reverse

Name: purU [H]

Synonym: GDI_1169

Alternate gene names: 162146964

Gene position: 1169801-1168872 (Counterclockwise)

Preceding gene: 162146965

Following gene: 162146963

Centisome position: 29.66

GC content: 64.19

Gene sequence:

>930_bases
GTGACGGACCAGGCTGGACGGACCAGGCTGGCGCGTGCCCGTGGATTGTCCGACATGACCCATCATCAGGTTCCGGCAAG
CTTTACAGTGACGCTGTCCTGTCCCAACCGGCCGGGCATCGTGGCGGCGATCGCCACCACGCTGTACGAAGCCGACGGCA
ATATTACCGAGGCGCAGCAGTTCGACGACGTCGAAACCGGATTGTTCTTCATGCGCGTCGTCTTCAGTATTCGTACGGAC
GGCGAGCGCCTGGACTGGCTGCGGGCGCGGCTGGGTGCCGTGGCCGAACGCTTTCACATGAACTGGACCCTGCATGACCG
CGCGGTGCGCCGCCGCGTCCTGCTGATGGTCTCGAAATTCGACCACTGCCTGGCCGACCTGCTGTACCGCTGGCGGATCG
GCGAGCTGCCGATGACGCCGACGGCCATCGTCGCCAATCATCCGCGCGCGGCCTATGGCCATATCGACATGGCCGATATC
CCGTTCCACCACCTGCCGGTCACGCGCGACAACAAGGCCGAGCAGGAAGAGCGCCTGTGGACCCTGGTGCGGCAGACCAA
TTCCGAACTGGTGGTGCTGGCCCGCTATATGCAGGTCCTGTCGGATTCCCTGACCGCGCGGCTGTCGGGGCGGTGCATCA
ACATCCACCATTCCTTCCTGCCGGGCTTCAAGGGCGCGCGGCCCTATCACCAGGCGCATGCGCGGGGGGTGAAGCTGATC
GGGGCGACGGCGCATTACGTGACAGCCGACCTGGATGAAGGCCCCATCATCGAACAGGATGTGGAACGCGTCAGCCATTT
CGATACGCCCGCCGACTTGGTGCGCAAGGGGCGTGACATCGAACGTCGCGTCCTGGCCCGCGCCGTACGCTATCACCTGG
ATGATCGTGTGATCCTGAACGGCAACAAGACGGTCGTGTTCGGTGATTGA

Upstream 100 bases:

>100_bases
TTCTGCGATACCATGCCCGACGCGCTGGAGGCCGCATGGAGGGCGGTGCGCGGCGCAGGTCCGTAGGGGCGGGCTTGAGT
TCCGGCGGCCGGCGCCTATT

Downstream 100 bases:

>100_bases
CGCGGAGGAGGTTGCTTTATCGTATTGACTGATCTGGCATATTCTCCGTAACGTTTCGTCATGATATTGTCCTGGGTGAT
GAATCGTTGCCCGGACGGGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTDQAGRTRLARARGLSDMTHHQVPASFTVTLSCPNRPGIVAAIATTLYEADGNITEAQQFDDVETGLFFMRVVFSIRTD
GERLDWLRARLGAVAERFHMNWTLHDRAVRRRVLLMVSKFDHCLADLLYRWRIGELPMTPTAIVANHPRAAYGHIDMADI
PFHHLPVTRDNKAEQEERLWTLVRQTNSELVVLARYMQVLSDSLTARLSGRCINIHHSFLPGFKGARPYHQAHARGVKLI
GATAHYVTADLDEGPIIEQDVERVSHFDTPADLVRKGRDIERRVLARAVRYHLDDRVILNGNKTVVFGD

Sequences:

>Translated_309_residues
MTDQAGRTRLARARGLSDMTHHQVPASFTVTLSCPNRPGIVAAIATTLYEADGNITEAQQFDDVETGLFFMRVVFSIRTD
GERLDWLRARLGAVAERFHMNWTLHDRAVRRRVLLMVSKFDHCLADLLYRWRIGELPMTPTAIVANHPRAAYGHIDMADI
PFHHLPVTRDNKAEQEERLWTLVRQTNSELVVLARYMQVLSDSLTARLSGRCINIHHSFLPGFKGARPYHQAHARGVKLI
GATAHYVTADLDEGPIIEQDVERVSHFDTPADLVRKGRDIERRVLARAVRYHLDDRVILNGNKTVVFGD
>Mature_308_residues
TDQAGRTRLARARGLSDMTHHQVPASFTVTLSCPNRPGIVAAIATTLYEADGNITEAQQFDDVETGLFFMRVVFSIRTDG
ERLDWLRARLGAVAERFHMNWTLHDRAVRRRVLLMVSKFDHCLADLLYRWRIGELPMTPTAIVANHPRAAYGHIDMADIP
FHHLPVTRDNKAEQEERLWTLVRQTNSELVVLARYMQVLSDSLTARLSGRCINIHHSFLPGFKGARPYHQAHARGVKLIG
ATAHYVTADLDEGPIIEQDVERVSHFDTPADLVRKGRDIERRVLARAVRYHLDDRVILNGNKTVVFGD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869995, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869993, Length=137, Percent_Identity=30.6569343065693, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=42.1428571428571, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788846, Length=114, Percent_Identity=31.5789473684211, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=163, Percent_Identity=32.5153374233129, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 35086; Mature: 34954

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQAGRTRLARARGLSDMTHHQVPASFTVTLSCPNRPGIVAAIATTLYEADGNITEAQQ
CCCCHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHH
FDDVETGLFFMRVVFSIRTDGERLDWLRARLGAVAERFHMNWTLHDRAVRRRVLLMVSKF
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
DHCLADLLYRWRIGELPMTPTAIVANHPRAAYGHIDMADIPFHHLPVTRDNKAEQEERLW
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TLVRQTNSELVVLARYMQVLSDSLTARLSGRCINIHHSFLPGFKGARPYHQAHARGVKLI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEE
GATAHYVTADLDEGPIIEQDVERVSHFDTPADLVRKGRDIERRVLARAVRYHLDDRVILN
ECEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC
GNKTVVFGD
CCEEEEECC
>Mature Secondary Structure 
TDQAGRTRLARARGLSDMTHHQVPASFTVTLSCPNRPGIVAAIATTLYEADGNITEAQQ
CCCHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHH
FDDVETGLFFMRVVFSIRTDGERLDWLRARLGAVAERFHMNWTLHDRAVRRRVLLMVSKF
HCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHH
DHCLADLLYRWRIGELPMTPTAIVANHPRAAYGHIDMADIPFHHLPVTRDNKAEQEERLW
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TLVRQTNSELVVLARYMQVLSDSLTARLSGRCINIHHSFLPGFKGARPYHQAHARGVKLI
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHCCEEEE
GATAHYVTADLDEGPIIEQDVERVSHFDTPADLVRKGRDIERRVLARAVRYHLDDRVILN
ECEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC
GNKTVVFGD
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]