Click here to switch to the map view.

The map label for this gene is dagK [H]

Identifier: 162146960

GI number: 162146960

Start: 1163694

End: 1164569

Strand: Direct

Name: dagK [H]

Synonym: GDI_1165

Alternate gene names: 162146960

Gene position: 1163694-1164569 (Clockwise)

Preceding gene: 162146959

Following gene: 162146962

Centisome position: 29.5

GC content: 69.52

Gene sequence:

>876_bases
ATGCGTATTCTGGTTATCTACAATCCCACGGCGGGACGGCGACGCCTGGGCCGGCTGCGACAGTTTGTCCGATGCCTGGA
GGCAGCGGGCGCGGCGGTAACGGTGGCGGCGACGGCCCATGCCGGGCATGGCCGTATCCTGTCGCGCGCCGCATGGCAGG
CAGGGACCGTTACCCATATCGTGGCGGCAGGCGGCGACGGTACGATTTCCGAGGTCGCCGACGGCCTGTCGGGGTCCGAC
CTGACCATGGGCGTATTGCCCCTGGGCACGGCCAACGTCCTGGCCCATGAACTCGGGATTCCGTTCGCGCCGGCCGACAA
CGCCCGGGCGCTGCTGTCGGGCGCATCTCGCACGATATGGCCGGGCCAGCTTCAGACCGGCACACAGACGTGCCTGTTCA
TGCAGATGGCCAGCGCGGGCTTCGATGCGCAGGTCGTGCATCACGTGTCATTGCGCGGCAAGCGCCTGCTGGGCCGCACC
GCCTATGCCGTGCAGGCCATCCGCGAAGCCGGCCGCTACGATTTCCCCCCGCTGCATGTCACGATCGACGGCGAGGCATG
CAGGACGTTCGGAGTCGTCGTCTCCAAGGGACGGTTCTATGGGGGGCGCCACGTCCTGGCGCCCGAAGCCACGCCCCTGC
GGCGCGGATTTTCCGTGACGTTGCTGGATGCGCCGGGGACGGGCCAGATGGCCAGGGCGGGGCTCGGCCTGCTGCGCGAC
CGCCCCGATCAGGTGCCGGGCATGCGGCGCGTCCGGGCGTCGCATATCCGCCTGATCGCGCCCGCCTGCGCCCCGGTCCA
GCATGACGGCGACGCATCGGGCCACCTGCCGCTTGTGATAAGCGATGCCCCGTTTCCGCTGCGCATCGCCGTATGA

Upstream 100 bases:

>100_bases
GCCGGCGGGAACCCAGGACGCATAAAATCCCCGCCCTGTCCCCCTTGGCCGCGTGGACACATCCGGGTACTGATCGACCG
GATCATCCTTGGCGGCCTGA

Downstream 100 bases:

>100_bases
TATCGGCCTGCCTTGCGACTGGTCATAACCGCACAGCGCGTGGCAGGCGCAGCAGGCCGGGCCGGGCGAAGGTCACGAGA
TTGCCCAGCAGGACCAGGAT

Product: diacylglycerol kinase family protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRILVIYNPTAGRRRLGRLRQFVRCLEAAGAAVTVAATAHAGHGRILSRAAWQAGTVTHIVAAGGDGTISEVADGLSGSD
LTMGVLPLGTANVLAHELGIPFAPADNARALLSGASRTIWPGQLQTGTQTCLFMQMASAGFDAQVVHHVSLRGKRLLGRT
AYAVQAIREAGRYDFPPLHVTIDGEACRTFGVVVSKGRFYGGRHVLAPEATPLRRGFSVTLLDAPGTGQMARAGLGLLRD
RPDQVPGMRRVRASHIRLIAPACAPVQHDGDASGHLPLVISDAPFPLRIAV

Sequences:

>Translated_291_residues
MRILVIYNPTAGRRRLGRLRQFVRCLEAAGAAVTVAATAHAGHGRILSRAAWQAGTVTHIVAAGGDGTISEVADGLSGSD
LTMGVLPLGTANVLAHELGIPFAPADNARALLSGASRTIWPGQLQTGTQTCLFMQMASAGFDAQVVHHVSLRGKRLLGRT
AYAVQAIREAGRYDFPPLHVTIDGEACRTFGVVVSKGRFYGGRHVLAPEATPLRRGFSVTLLDAPGTGQMARAGLGLLRD
RPDQVPGMRRVRASHIRLIAPACAPVQHDGDASGHLPLVISDAPFPLRIAV
>Mature_291_residues
MRILVIYNPTAGRRRLGRLRQFVRCLEAAGAAVTVAATAHAGHGRILSRAAWQAGTVTHIVAAGGDGTISEVADGLSGSD
LTMGVLPLGTANVLAHELGIPFAPADNARALLSGASRTIWPGQLQTGTQTCLFMQMASAGFDAQVVHHVSLRGKRLLGRT
AYAVQAIREAGRYDFPPLHVTIDGEACRTFGVVVSKGRFYGGRHVLAPEATPLRRGFSVTLLDAPGTGQMARAGLGLLRD
RPDQVPGMRRVRASHIRLIAPACAPVQHDGDASGHLPLVISDAPFPLRIAV

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=201, Percent_Identity=31.8407960199005, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 30658; Mature: 30658

Theoretical pI: Translated: 11.30; Mature: 11.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVIYNPTAGRRRLGRLRQFVRCLEAAGAAVTVAATAHAGHGRILSRAAWQAGTVTHI
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEE
VAAGGDGTISEVADGLSGSDLTMGVLPLGTANVLAHELGIPFAPADNARALLSGASRTIW
EEECCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCC
PGQLQTGTQTCLFMQMASAGFDAQVVHHVSLRGKRLLGRTAYAVQAIREAGRYDFPPLHV
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
TIDGEACRTFGVVVSKGRFYGGRHVLAPEATPLRRGFSVTLLDAPGTGQMARAGLGLLRD
EECCHHHHEEEEEEECCEECCCEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHC
RPDQVPGMRRVRASHIRLIAPACAPVQHDGDASGHLPLVISDAPFPLRIAV
CCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEC
>Mature Secondary Structure
MRILVIYNPTAGRRRLGRLRQFVRCLEAAGAAVTVAATAHAGHGRILSRAAWQAGTVTHI
CEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEE
VAAGGDGTISEVADGLSGSDLTMGVLPLGTANVLAHELGIPFAPADNARALLSGASRTIW
EEECCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCC
PGQLQTGTQTCLFMQMASAGFDAQVVHHVSLRGKRLLGRTAYAVQAIREAGRYDFPPLHV
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
TIDGEACRTFGVVVSKGRFYGGRHVLAPEATPLRRGFSVTLLDAPGTGQMARAGLGLLRD
EECCHHHHEEEEEEECCEECCCEEEECCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHC
RPDQVPGMRRVRASHIRLIAPACAPVQHDGDASGHLPLVISDAPFPLRIAV
CCCCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]