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The map label for this gene is ribE [H]

Identifier: 162146948

GI number: 162146948

Start: 1148884

End: 1149501

Strand: Reverse

Name: ribE [H]

Synonym: GDI_1153

Alternate gene names: 162146948

Gene position: 1149501-1148884 (Counterclockwise)

Preceding gene: 162146949

Following gene: 162146947

Centisome position: 29.14

GC content: 67.15

Gene sequence:

>618_bases
ATGTTTTCCGGCATCATAGAGCATCTGGGCACCGTCACGGCGGCGCGGCGCGGCGACCGGTCGCTGCATGTCGCGGTGGC
CACGGGCATCGGCGACCTGTCGATGGGCGAGAGCATCGCCGTCAACGGCGTGTGCCTGACGGTGACCGCCTATGACGCGG
CGGGCAATGCCTCCTTCTTCATCAGTTCGGAGACCCTGGAGCGCACCAGCCTGGGCCGCCTGGCCGAAGGCAGCCGCGTG
AACCTGGAACGCGCCGTCACGCCGGCCACGCGCCTGTCGGGCCATATCGTGCAGGGCCATGTGGACGGCACGGCGCGCCT
GCTGTCGGTCGAGGATGCGGGCGAATCCCGGCGCATCGTCTTCGGCATCCCGGCCGCGCTGCGCCGCTATCTGGTCGAGA
AGGGTTCGATCACCCTGGACGGCATCAGCCTGACGCTGAACGAGATCGGCGAGGTCGGAACGGGTGCGGGCGGCGCGGAG
GAATTCCCCGTCGCGCTGATGATCATTCCCCATACCTGGACCCATACCACGCTGGGCACCCTGTCGCCCGGCGATGGCGT
GAATGTCGAGGTCGATGTCATCGCGAAATATGTGGAGACCCTATGCCAGTACCGGTGA

Upstream 100 bases:

>100_bases
ATGTCGCCGTCCGCTGCGGCACGGATACGCCGCTGCGCCTGTTCGCGGAACTGGCGGCGCCGGGGACGGCACATCCAGGG
ACGGCACATGGGGAGGAAGC

Downstream 100 bases:

>100_bases
TGAGTCCCGGCCTGCGGACCGCGGTGGCGGCGATCCGGGCGGGCGGCATGGTGATCCTGGTCGATGACGAGGATCGCGAG
AACGAGGGCGACCTGGTGAT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MFSGIIEHLGTVTAARRGDRSLHVAVATGIGDLSMGESIAVNGVCLTVTAYDAAGNASFFISSETLERTSLGRLAEGSRV
NLERAVTPATRLSGHIVQGHVDGTARLLSVEDAGESRRIVFGIPAALRRYLVEKGSITLDGISLTLNEIGEVGTGAGGAE
EFPVALMIIPHTWTHTTLGTLSPGDGVNVEVDVIAKYVETLCQYR

Sequences:

>Translated_205_residues
MFSGIIEHLGTVTAARRGDRSLHVAVATGIGDLSMGESIAVNGVCLTVTAYDAAGNASFFISSETLERTSLGRLAEGSRV
NLERAVTPATRLSGHIVQGHVDGTARLLSVEDAGESRRIVFGIPAALRRYLVEKGSITLDGISLTLNEIGEVGTGAGGAE
EFPVALMIIPHTWTHTTLGTLSPGDGVNVEVDVIAKYVETLCQYR
>Mature_205_residues
MFSGIIEHLGTVTAARRGDRSLHVAVATGIGDLSMGESIAVNGVCLTVTAYDAAGNASFFISSETLERTSLGRLAEGSRV
NLERAVTPATRLSGHIVQGHVDGTARLLSVEDAGESRRIVFGIPAALRRYLVEKGSITLDGISLTLNEIGEVGTGAGGAE
EFPVALMIIPHTWTHTTLGTLSPGDGVNVEVDVIAKYVETLCQYR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=198, Percent_Identity=35.8585858585859, Blast_Score=94, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319733, Length=213, Percent_Identity=38.9671361502347, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 21534; Mature: 21534

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGIIEHLGTVTAARRGDRSLHVAVATGIGDLSMGESIAVNGVCLTVTAYDAAGNASFF
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCEEEEEEEECCCCCEEEE
ISSETLERTSLGRLAEGSRVNLERAVTPATRLSGHIVQGHVDGTARLLSVEDAGESRRIV
EECCHHHHHHCCCCCCCCCCCHHHHCCCHHHHCCEEEEEEECCCEEEEEEECCCCCCEEE
FGIPAALRRYLVEKGSITLDGISLTLNEIGEVGTGAGGAEEFPVALMIIPHTWTHTTLGT
EECHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
LSPGDGVNVEVDVIAKYVETLCQYR
CCCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSGIIEHLGTVTAARRGDRSLHVAVATGIGDLSMGESIAVNGVCLTVTAYDAAGNASFF
CCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEECCEEEEEEEECCCCCEEEE
ISSETLERTSLGRLAEGSRVNLERAVTPATRLSGHIVQGHVDGTARLLSVEDAGESRRIV
EECCHHHHHHCCCCCCCCCCCHHHHCCCHHHHCCEEEEEEECCCEEEEEEECCCCCCEEE
FGIPAALRRYLVEKGSITLDGISLTLNEIGEVGTGAGGAEEFPVALMIIPHTWTHTTLGT
EECHHHHHHHHHHCCCEEEEEEEEEHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCC
LSPGDGVNVEVDVIAKYVETLCQYR
CCCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]