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The map label for this gene is ydgK [H]

Identifier: 162146936

GI number: 162146936

Start: 1134105

End: 1135349

Strand: Reverse

Name: ydgK [H]

Synonym: GDI_1141

Alternate gene names: 162146936

Gene position: 1135349-1134105 (Counterclockwise)

Preceding gene: 162146937

Following gene: 162146934

Centisome position: 28.79

GC content: 69.72

Gene sequence:

>1245_bases
TTGCCCATGTCTTCCTCCACATCCTCGCTTGCCTATCGCCATCGTATTCCCGTCGGCGCGCTGCCGGCGTGGCTGGTGAT
TCTGCTGGGCCTGGTCACGGCGATCGGGCCGCTGGCCACCGACATGTACCTGCCGGCCTTTCCGGCGGTCGATCGCGACC
TGGGCGGCGGTCCGGGCTCGGCGCAGATCACGCTGGCATCGTGGTTCGCGGGGCTGGCGATCGGCCAGTTCAGCCAGGGC
CCCCTGTCGGACCGGCTGGGGCGGCGGGTGCCGCTGCTGGGCGGCCTGACGCTGTTCAGCATCGCCTCGGCGGGGTGTGC
GCTGTCGTACAGCTATCACATGTTCTGCGTCTGGCGCTTCCTGGCCGCCCTTGGCGGCTCGGCCGGCGTGGTGGTGCCGC
GCGCCATCGTGCGGGACGTCGCGACCGGCATGCAGGGCGCGCGGATCATGGCGCAACTGACCCTGGTCTTCGGCCTGATG
CCGATCCTGGCCCCCAGCCTGGGCAGCCTGGTGCTGTCGGTCGGCCACTGGCGCTGGATCTTCTGGTTCGCGGTCGTCTA
TGGCGCGCTGGGTGTCGTTGCCATCGCCTGGACGCTGCCCGACACCATGCCGCGCGACCGGCGCATCGCGCTGTCGATCC
CGACCGTGCTCTGGCGCTATGTCACCATCGCGCGCGAGCCGATGTTCCTGTCGGCGGCGCTGCTGCTGGGCTTCTCGGCC
TTCGTGATGTTCGGCTACCTGGCCAGCGCGCCGACGCTGTTCGAACACATCCTGTCCTTCACCCCGGGGCAGTTCGGCCT
GTTCTTCGGCGTGAATGCCGCGCTGTTCATCACCGGGGCGCAGGTCAACGGGCGGCTGGTCCACCGGGTGGCGCTGGATG
TGCTGATGCTGCGCGGCGTGACGGTGGTGACGATGGCGGCCGTTCTGTTCCTGGCCCTGTCCCTGGCCGGAATTGCGGGG
CCGCGCCATCCGGCGCTGGTGTGCTGCCTGATCGGGCTGATCACGGGCTCGCTGGGGTTCGTCAACCCCAATGCCACGGT
GCTGGCCTTCACCCGGCACGGCCATCATGCCGGCAGCGCCTCGGCCCTGCTGGGCACGCTGCAATTCACCATCGGCTCGC
TCAGCGGCGTGCTGCTGGGCTACCTGCCGCTGGATACGACGGTGCCGATCGCCGGTACCATGGCGGCGGGCATCGTCGGC
ATGCTGTTCTGCACCGTGTGGCACCGGCGGCATTCGGCGGGCTGA

Upstream 100 bases:

>100_bases
TCTGCCATGTTTCCGGCGGGGCGCGCCGTGAGGGATAGCAATGGACAAGTGTCGCGGAAACCGTGACATACGGTGCCCCT
TGTTCATCGGGTTCACGACC

Downstream 100 bases:

>100_bases
AGGTCAGGGCCGTCCTACGGAATGATAGGCGAACCCTGCTTCCTGCATCGCCACGGGGTCGAAGATATTGCGCAGGTCGA
CGACCACGCGGCCCCGCATC

Product: bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MPMSSSTSSLAYRHRIPVGALPAWLVILLGLVTAIGPLATDMYLPAFPAVDRDLGGGPGSAQITLASWFAGLAIGQFSQG
PLSDRLGRRVPLLGGLTLFSIASAGCALSYSYHMFCVWRFLAALGGSAGVVVPRAIVRDVATGMQGARIMAQLTLVFGLM
PILAPSLGSLVLSVGHWRWIFWFAVVYGALGVVAIAWTLPDTMPRDRRIALSIPTVLWRYVTIAREPMFLSAALLLGFSA
FVMFGYLASAPTLFEHILSFTPGQFGLFFGVNAALFITGAQVNGRLVHRVALDVLMLRGVTVVTMAAVLFLALSLAGIAG
PRHPALVCCLIGLITGSLGFVNPNATVLAFTRHGHHAGSASALLGTLQFTIGSLSGVLLGYLPLDTTVPIAGTMAAGIVG
MLFCTVWHRRHSAG

Sequences:

>Translated_414_residues
MPMSSSTSSLAYRHRIPVGALPAWLVILLGLVTAIGPLATDMYLPAFPAVDRDLGGGPGSAQITLASWFAGLAIGQFSQG
PLSDRLGRRVPLLGGLTLFSIASAGCALSYSYHMFCVWRFLAALGGSAGVVVPRAIVRDVATGMQGARIMAQLTLVFGLM
PILAPSLGSLVLSVGHWRWIFWFAVVYGALGVVAIAWTLPDTMPRDRRIALSIPTVLWRYVTIAREPMFLSAALLLGFSA
FVMFGYLASAPTLFEHILSFTPGQFGLFFGVNAALFITGAQVNGRLVHRVALDVLMLRGVTVVTMAAVLFLALSLAGIAG
PRHPALVCCLIGLITGSLGFVNPNATVLAFTRHGHHAGSASALLGTLQFTIGSLSGVLLGYLPLDTTVPIAGTMAAGIVG
MLFCTVWHRRHSAG
>Mature_413_residues
PMSSSTSSLAYRHRIPVGALPAWLVILLGLVTAIGPLATDMYLPAFPAVDRDLGGGPGSAQITLASWFAGLAIGQFSQGP
LSDRLGRRVPLLGGLTLFSIASAGCALSYSYHMFCVWRFLAALGGSAGVVVPRAIVRDVATGMQGARIMAQLTLVFGLMP
ILAPSLGSLVLSVGHWRWIFWFAVVYGALGVVAIAWTLPDTMPRDRRIALSIPTVLWRYVTIAREPMFLSAALLLGFSAF
VMFGYLASAPTLFEHILSFTPGQFGLFFGVNAALFITGAQVNGRLVHRVALDVLMLRGVTVVTMAAVLFLALSLAGIAGP
RHPALVCCLIGLITGSLGFVNPNATVLAFTRHGHHAGSASALLGTLQFTIGSLSGVLLGYLPLDTTVPIAGTMAAGIVGM
LFCTVWHRRHSAG

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=381, Percent_Identity=30.7086614173228, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI48994889, Length=378, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790146, Length=292, Percent_Identity=27.3972602739726, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790794, Length=229, Percent_Identity=26.6375545851528, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082312, Length=171, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787065, Length=266, Percent_Identity=23.6842105263158, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43598; Mature: 43467

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSSSTSSLAYRHRIPVGALPAWLVILLGLVTAIGPLATDMYLPAFPAVDRDLGGGPGS
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AQITLASWFAGLAIGQFSQGPLSDRLGRRVPLLGGLTLFSIASAGCALSYSYHMFCVWRF
CEEEHHHHHHHHHHCCCCCCCCHHHHCCCCCHHCCHHHHHHHHCCHHEEHHHHHHHHHHH
LAALGGSAGVVVPRAIVRDVATGMQGARIMAQLTLVFGLMPILAPSLGSLVLSVGHWRWI
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWFAVVYGALGVVAIAWTLPDTMPRDRRIALSIPTVLWRYVTIAREPMFLSAALLLGFSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVMFGYLASAPTLFEHILSFTPGQFGLFFGVNAALFITGAQVNGRLVHRVALDVLMLRGV
HHHHHHHHCCHHHHHHHHHCCCCCEEHHEECCEEEEEEECCCCCHHHHHHHHHHHHHHHH
TVVTMAAVLFLALSLAGIAGPRHPALVCCLIGLITGSLGFVNPNATVLAFTRHGHHAGSA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCH
SALLGTLQFTIGSLSGVLLGYLPLDTTVPIAGTMAAGIVGMLFCTVWHRRHSAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMSSSTSSLAYRHRIPVGALPAWLVILLGLVTAIGPLATDMYLPAFPAVDRDLGGGPGS
CCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
AQITLASWFAGLAIGQFSQGPLSDRLGRRVPLLGGLTLFSIASAGCALSYSYHMFCVWRF
CEEEHHHHHHHHHHCCCCCCCCHHHHCCCCCHHCCHHHHHHHHCCHHEEHHHHHHHHHHH
LAALGGSAGVVVPRAIVRDVATGMQGARIMAQLTLVFGLMPILAPSLGSLVLSVGHWRWI
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWFAVVYGALGVVAIAWTLPDTMPRDRRIALSIPTVLWRYVTIAREPMFLSAALLLGFSA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVMFGYLASAPTLFEHILSFTPGQFGLFFGVNAALFITGAQVNGRLVHRVALDVLMLRGV
HHHHHHHHCCHHHHHHHHHCCCCCEEHHEECCEEEEEEECCCCCHHHHHHHHHHHHHHHH
TVVTMAAVLFLALSLAGIAGPRHPALVCCLIGLITGSLGFVNPNATVLAFTRHGHHAGSA
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCH
SALLGTLQFTIGSLSGVLLGYLPLDTTVPIAGTMAAGIVGMLFCTVWHRRHSAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]