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The map label for this gene is hldE

Identifier: 162146928

GI number: 162146928

Start: 1124467

End: 1125921

Strand: Direct

Name: hldE

Synonym: GDI_1133

Alternate gene names: 162146928

Gene position: 1124467-1125921 (Clockwise)

Preceding gene: 162146927

Following gene: 162146929

Centisome position: 28.51

GC content: 72.1

Gene sequence:

>1455_bases
ATGGATTTTTCATCGATCACGGTGCTGTGCATCGGGGACATCATGCTGGACCGTTTCGTCTATGGCGAAATGGAGCGCAT
TTCCCCCGAGGCCCCGGTGCCCGTCCTGCGGCTGGGCCGCACGCGCGAAATGCCCGGCGGCGTGGGCAACGTGGCCAACA
ACATCCTGTCGCTGGGCGGCCGGGCGATCCTGGTCGGCCTGGTGGGACGGGACACGCCGGGGCAGGCCCTGCGCGGCCTG
CTGGGCCAGCGCCCGGGCCTGACCGACGCACTGGTCGAAACCGGCGCGCGGCCGACGATCTGCAAGACGCGCTTCATCGC
CGCCAACCAGCAGGTCGTCCGCGCGGACGAGGAAAGCCGCCTGGCGCTGCAACCCGACGAGGCCACGTCCCTGATCGCCG
CCATTGACGCCCATATCGGCGCGGCGGACATCGTCGTGCTGTCGGACTATGCCAAGGGAACCTGCGCCGACCCGGTGATC
GCCCATGCCATCGCCACGGCGCGGGCGCGGGGGATCGCGGTGTTCGTCGATCCGAAATCGCGCGATTTCGCACGCTATCG
CGGGGCGAGCTGCATCACGCCCAACGCCCGTGAACTGGCCCAGGCCACGGGCCTGCCCATCGATACCGACGCCGAGATCG
AGCAGGCGGCCCGCGCGGCGATGGCCCGGGCCGACGCCGTCGCCATCCTGGCCACGCGGTCCGAGAAGGGCATGGCGCTG
GTCGAGCGCGAGGGCGGGGTCCAGATCGTCCCGGCCCGCGCGCGCGAGGTCTTCGACGTGTCGGGCGCGGGCGACACCGT
GATCGCCGCCCTGGCGCTGGCCGCGGGATCGGGCATGACGCTGGCCCAGGCGATGCACGTGGCCAATGCCGCCGCCGGGG
TCGTGGTCGGCAAGCTGGGCACCGCGACGGCCGACATCGCCGAGGTCCTGGCCGAACTGAACGCCCAGGACGCCGATGCC
ATGGGCGGCACGCCGTCCCTGCTGTCGCGCGCGCAGGCGGCCGAGCAGGTCGCGCGCTGGAAGGCGCAGGGCCTGCGGGT
CGGCTTCACCAACGGCTGTTTCGACATCATCCATCCCGGCCATGTCGCGCTGCTGGCGGCGGCCCGGAGGGCATGCGACC
GGCTGATCGTGGCGCTGAACGACGATGCCAGCGTGGCGCGGCTGAAGGGCCCCGAACGCCCGGTCAACCCGCTGGAAGAC
CGGGCCCGGGTCATGGCGGCGATCCGCCACGTCGATGCCGTCGTCGCCTTCGGCGAGGACACGCCGCTGGAGTTGATCCG
GCTGCTGTTGCCCGACGTGCTGGTCAAGGGCGCGGACTATCGCCCCGACCAGGTCGTGGGCGCGGACGTGGTGCGGCAGG
CCGGCGGGCGCGTGGTGCTGGCCGACCTGGAAGACGGCAAATCGACCACCCGCACGATCGGCCGCATCCGTGCCGCCGGG
GCGGCCGACCGATGA

Upstream 100 bases:

>100_bases
GATCGGCGAGGCCCGGTCGGTCCTGACGGCAGCGCGGCTGCAGACCCGGCGGCAGGCAGGGCTGGCGGCCTTCGTCGCCG
GATTCGGCGCGTAGCCGGCC

Downstream 100 bases:

>100_bases
CCGCCGACGGAATGGCCCCCGTCACCCCCCCCGCCGTGGCGGACGACAAAATCCGCCAGGCCCATGCCGCGTTCCGGTCG
TGGCTGTTCGAGACCGCCCT

Product: bifunctional protein HldE kinase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDFSSITVLCIGDIMLDRFVYGEMERISPEAPVPVLRLGRTREMPGGVGNVANNILSLGGRAILVGLVGRDTPGQALRGL
LGQRPGLTDALVETGARPTICKTRFIAANQQVVRADEESRLALQPDEATSLIAAIDAHIGAADIVVLSDYAKGTCADPVI
AHAIATARARGIAVFVDPKSRDFARYRGASCITPNARELAQATGLPIDTDAEIEQAARAAMARADAVAILATRSEKGMAL
VEREGGVQIVPARAREVFDVSGAGDTVIAALALAAGSGMTLAQAMHVANAAAGVVVGKLGTATADIAEVLAELNAQDADA
MGGTPSLLSRAQAAEQVARWKAQGLRVGFTNGCFDIIHPGHVALLAAARRACDRLIVALNDDASVARLKGPERPVNPLED
RARVMAAIRHVDAVVAFGEDTPLELIRLLLPDVLVKGADYRPDQVVGADVVRQAGGRVVLADLEDGKSTTRTIGRIRAAG
AADR

Sequences:

>Translated_484_residues
MDFSSITVLCIGDIMLDRFVYGEMERISPEAPVPVLRLGRTREMPGGVGNVANNILSLGGRAILVGLVGRDTPGQALRGL
LGQRPGLTDALVETGARPTICKTRFIAANQQVVRADEESRLALQPDEATSLIAAIDAHIGAADIVVLSDYAKGTCADPVI
AHAIATARARGIAVFVDPKSRDFARYRGASCITPNARELAQATGLPIDTDAEIEQAARAAMARADAVAILATRSEKGMAL
VEREGGVQIVPARAREVFDVSGAGDTVIAALALAAGSGMTLAQAMHVANAAAGVVVGKLGTATADIAEVLAELNAQDADA
MGGTPSLLSRAQAAEQVARWKAQGLRVGFTNGCFDIIHPGHVALLAAARRACDRLIVALNDDASVARLKGPERPVNPLED
RARVMAAIRHVDAVVAFGEDTPLELIRLLLPDVLVKGADYRPDQVVGADVVRQAGGRVVLADLEDGKSTTRTIGRIRAAG
AADR
>Mature_484_residues
MDFSSITVLCIGDIMLDRFVYGEMERISPEAPVPVLRLGRTREMPGGVGNVANNILSLGGRAILVGLVGRDTPGQALRGL
LGQRPGLTDALVETGARPTICKTRFIAANQQVVRADEESRLALQPDEATSLIAAIDAHIGAADIVVLSDYAKGTCADPVI
AHAIATARARGIAVFVDPKSRDFARYRGASCITPNARELAQATGLPIDTDAEIEQAARAAMARADAVAILATRSEKGMAL
VEREGGVQIVPARAREVFDVSGAGDTVIAALALAAGSGMTLAQAMHVANAAAGVVVGKLGTATADIAEVLAELNAQDADA
MGGTPSLLSRAQAAEQVARWKAQGLRVGFTNGCFDIIHPGHVALLAAARRACDRLIVALNDDASVARLKGPERPVNPLED
RARVMAAIRHVDAVVAFGEDTPLELIRLLLPDVLVKGADYRPDQVVGADVVRQAGGRVVLADLEDGKSTTRTIGRIRAAG
AADR

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=477, Percent_Identity=44.8637316561845, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_GLUDA (A9HDB1)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001601389.1
- RefSeq:   YP_002276221.1
- ProteinModelPortal:   A9HDB1
- SMR:   A9HDB1
- GeneID:   5790441
- GeneID:   6975268
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1846
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   CLSK958018
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50453; Mature: 50453

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSSITVLCIGDIMLDRFVYGEMERISPEAPVPVLRLGRTREMPGGVGNVANNILSLGG
CCCCCEEEEEHHHHHHHHHHHCHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCC
RAILVGLVGRDTPGQALRGLLGQRPGLTDALVETGARPTICKTRFIAANQQVVRADEESR
CEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHEEEECCCHHEECCCCCC
LALQPDEATSLIAAIDAHIGAADIVVLSDYAKGTCADPVIAHAIATARARGIAVFVDPKS
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCC
RDFARYRGASCITPNARELAQATGLPIDTDAEIEQAARAAMARADAVAILATRSEKGMAL
CHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCEE
VEREGGVQIVPARAREVFDVSGAGDTVIAALALAAGSGMTLAQAMHVANAAAGVVVGKLG
EEECCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCC
TATADIAEVLAELNAQDADAMGGTPSLLSRAQAAEQVARWKAQGLRVGFTNGCFDIIHPG
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCC
HVALLAAARRACDRLIVALNDDASVARLKGPERPVNPLEDRARVMAAIRHVDAVVAFGED
HHHHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
TPLELIRLLLPDVLVKGADYRPDQVVGADVVRQAGGRVVLADLEDGKSTTRTIGRIRAAG
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCC
AADR
CCCC
>Mature Secondary Structure
MDFSSITVLCIGDIMLDRFVYGEMERISPEAPVPVLRLGRTREMPGGVGNVANNILSLGG
CCCCCEEEEEHHHHHHHHHHHCHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCC
RAILVGLVGRDTPGQALRGLLGQRPGLTDALVETGARPTICKTRFIAANQQVVRADEESR
CEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHEEEECCCHHEECCCCCC
LALQPDEATSLIAAIDAHIGAADIVVLSDYAKGTCADPVIAHAIATARARGIAVFVDPKS
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECCCC
RDFARYRGASCITPNARELAQATGLPIDTDAEIEQAARAAMARADAVAILATRSEKGMAL
CHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEEEEECCCCCCEE
VEREGGVQIVPARAREVFDVSGAGDTVIAALALAAGSGMTLAQAMHVANAAAGVVVGKLG
EEECCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEECCC
TATADIAEVLAELNAQDADAMGGTPSLLSRAQAAEQVARWKAQGLRVGFTNGCFDIIHPG
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCC
HVALLAAARRACDRLIVALNDDASVARLKGPERPVNPLEDRARVMAAIRHVDAVVAFGED
HHHHHHHHHHHHHEEEEEECCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCC
TPLELIRLLLPDVLVKGADYRPDQVVGADVVRQAGGRVVLADLEDGKSTTRTIGRIRAAG
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCC
AADR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA