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The map label for this gene is oprJ [H]

Identifier: 162146815

GI number: 162146815

Start: 1012152

End: 1013693

Strand: Reverse

Name: oprJ [H]

Synonym: GDI_0997

Alternate gene names: 162146815

Gene position: 1013693-1012152 (Counterclockwise)

Preceding gene: 162146816

Following gene: 162146814

Centisome position: 25.7

GC content: 68.22

Gene sequence:

>1542_bases
ATGCTCGCTCTGGCGAGCGCGCTCCTGCTCGGCGCCTGCACGGTCGGGCCCGACTATCACAAGCCGGATCTCGCCAGCGC
GCCGCACTGGCGCCGCGGTATGGCGGATGCCGAAAGCCACCCGGTGGAAAGCCCGGCCGATCCGCAATGGTGGACGATCT
ATCGCGATCCGGTGCTGACGGCGCTCGAACAGCAGGTCGCGTCGGCCAATCTGGATCTGCGGGCAAGCGCCTATCGCCTG
GCGGAGAGCATCGCCGAGCGGCGGATCGCGAGCGCGGCACAGTTCCCGCATATGGAGGGGAATGCGTCCTACGCCCGCGA
GCGGGCCAGCACCAACGGTATCCTCGGCCTGCTCGGCACGCTGGAGCGCGCCGGCGCGGGCGATGTCGCCAGCGGCGCGC
AGGGCTTCGGCCCCGTGGCTGTGCCGGGCACGGTCGGCAATCCGTCCTTCAATCTGCCGCAATACGGCATGAGCGCGTCC
TGGGAAGTCGATCTCTGGGGTCATGTTCGGCGTCAGGTCGAGGCGGCCAACGCGGCTGTGCAGGCGACGCAGGACATGCA
GCGCGACGTGCTGGTCTCGCTGATGGCCGAGACCGCGCAGGACTATCTCGATCTGCGCGGCGTCCAGGCACAGATCGCCA
TCGTCGACCGCAATATCGACATCGCGCGGCATAGTGTCGATTTGACGGCGATGCGCTTTACGCAGGGAGCCTCCACGCAG
CTCGACGTGGCGGATTCGCGCGGACAACTCAGCGAATTCCAGTCGCGCCTGCCGATCCTGCGCAGCCAGGAAGTGCATCT
GATCAACGCGCTGAGCTTTCTCGTTGCGCGCGAGCCGGGTGCGCTCCGGGCGCAACTCGGCACGCCTGCCCCGATCCCGC
CGGTACCCGATGCGGTGCCCGTTGGCCTGCCGTCGGAACTGGCCGAACGTCGGCCCGATATCCGCATGGCCGAGGAGCAG
TTGCATGTCGCCACCGCCAGCATCGGCGTGGCCGTGGCGGATTTCTTTCCGCGCGTGACGCTGTCCGGCAGTCTCGACGT
GCAGGCGCTGCAATTCAGCGGGCTCGGCTCCTGGGCGTCGCGACAATACGGCTTCGGTCCGACCATGACGCTGCCGCTGT
TCGAGGGTGGCCGCCTCACCGGGCAATTGCGCCTACGCCGGGCGCAGCAGAAGGAAGCGGCGATCACCCTGCAGCGCACC
ATTCTCAAGGCCTGGCAGGAGATCGATGACGCGATGGCCGATTTCTCCGCCGCCCAGCAGCAGCGCGACCGGCTGAAGGA
GACCGTGGCCCAGAACGAGACGGCCGTGCGCCTGGCCCAGCAGCAATACGTGCAGGGGGCGTCGGACTTCTTGAACGTGC
TGACCCTGCAGAACGCGCTTCTGGCCAGCCAGAACCAGCTCGTCCAGTCGACGACGGCGGTTTCGGTCGCAGTGGCCCGG
CTTTACCGCGCGCTTGGTGGTGGCTGGGAGGCGCGCTATCCGGAAGCGGTGGCCCGGCGGTCGACCATCCAGTCAGCGGG
GCAGGGAGGGCGACAGGGATGA

Upstream 100 bases:

>100_bases
AGGGTCGACACGGCCTCCCGTCCCGAAGGCCCGAAGGCGGACGACCCCCGCTATGCTTGGCGGTGAGGGGCGGATCATGA
TCACACGCCTCAACCGCCCC

Downstream 100 bases:

>100_bases
TCAAAGTGCGGCTGCCTTCCGAGCTGGGTCATCTGGTCCAGGATGGCGTCAAGCTCGAGTGCCATTTCGCATTCCGGGAC
TATGCTCACCCCGCTCCCTC

Product: outer membrane protein oprJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MLALASALLLGACTVGPDYHKPDLASAPHWRRGMADAESHPVESPADPQWWTIYRDPVLTALEQQVASANLDLRASAYRL
AESIAERRIASAAQFPHMEGNASYARERASTNGILGLLGTLERAGAGDVASGAQGFGPVAVPGTVGNPSFNLPQYGMSAS
WEVDLWGHVRRQVEAANAAVQATQDMQRDVLVSLMAETAQDYLDLRGVQAQIAIVDRNIDIARHSVDLTAMRFTQGASTQ
LDVADSRGQLSEFQSRLPILRSQEVHLINALSFLVAREPGALRAQLGTPAPIPPVPDAVPVGLPSELAERRPDIRMAEEQ
LHVATASIGVAVADFFPRVTLSGSLDVQALQFSGLGSWASRQYGFGPTMTLPLFEGGRLTGQLRLRRAQQKEAAITLQRT
ILKAWQEIDDAMADFSAAQQQRDRLKETVAQNETAVRLAQQQYVQGASDFLNVLTLQNALLASQNQLVQSTTAVSVAVAR
LYRALGGGWEARYPEAVARRSTIQSAGQGGRQG

Sequences:

>Translated_513_residues
MLALASALLLGACTVGPDYHKPDLASAPHWRRGMADAESHPVESPADPQWWTIYRDPVLTALEQQVASANLDLRASAYRL
AESIAERRIASAAQFPHMEGNASYARERASTNGILGLLGTLERAGAGDVASGAQGFGPVAVPGTVGNPSFNLPQYGMSAS
WEVDLWGHVRRQVEAANAAVQATQDMQRDVLVSLMAETAQDYLDLRGVQAQIAIVDRNIDIARHSVDLTAMRFTQGASTQ
LDVADSRGQLSEFQSRLPILRSQEVHLINALSFLVAREPGALRAQLGTPAPIPPVPDAVPVGLPSELAERRPDIRMAEEQ
LHVATASIGVAVADFFPRVTLSGSLDVQALQFSGLGSWASRQYGFGPTMTLPLFEGGRLTGQLRLRRAQQKEAAITLQRT
ILKAWQEIDDAMADFSAAQQQRDRLKETVAQNETAVRLAQQQYVQGASDFLNVLTLQNALLASQNQLVQSTTAVSVAVAR
LYRALGGGWEARYPEAVARRSTIQSAGQGGRQG
>Mature_513_residues
MLALASALLLGACTVGPDYHKPDLASAPHWRRGMADAESHPVESPADPQWWTIYRDPVLTALEQQVASANLDLRASAYRL
AESIAERRIASAAQFPHMEGNASYARERASTNGILGLLGTLERAGAGDVASGAQGFGPVAVPGTVGNPSFNLPQYGMSAS
WEVDLWGHVRRQVEAANAAVQATQDMQRDVLVSLMAETAQDYLDLRGVQAQIAIVDRNIDIARHSVDLTAMRFTQGASTQ
LDVADSRGQLSEFQSRLPILRSQEVHLINALSFLVAREPGALRAQLGTPAPIPPVPDAVPVGLPSELAERRPDIRMAEEQ
LHVATASIGVAVADFFPRVTLSGSLDVQALQFSGLGSWASRQYGFGPTMTLPLFEGGRLTGQLRLRRAQQKEAAITLQRT
ILKAWQEIDDAMADFSAAQQQRDRLKETVAQNETAVRLAQQQYVQGASDFLNVLTLQNALLASQNQLVQSTTAVSVAVAR
LYRALGGGWEARYPEAVARRSTIQSAGQGGRQG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=491, Percent_Identity=24.847250509165, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI2367348, Length=443, Percent_Identity=23.7020316027088, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55282; Mature: 55282

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALASALLLGACTVGPDYHKPDLASAPHWRRGMADAESHPVESPADPQWWTIYRDPVLT
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCHHHH
ALEQQVASANLDLRASAYRLAESIAERRIASAAQFPHMEGNASYARERASTNGILGLLGT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
LERAGAGDVASGAQGFGPVAVPGTVGNPSFNLPQYGMSASWEVDLWGHVRRQVEAANAAV
HHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
QATQDMQRDVLVSLMAETAQDYLDLRGVQAQIAIVDRNIDIARHSVDLTAMRFTQGASTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCEEEEECCCCCCE
LDVADSRGQLSEFQSRLPILRSQEVHLINALSFLVAREPGALRAQLGTPAPIPPVPDAVP
EECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCC
VGLPSELAERRPDIRMAEEQLHVATASIGVAVADFFPRVTLSGSLDVQALQFSGLGSWAS
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCHHH
RQYGFGPTMTLPLFEGGRLTGQLRLRRAQQKEAAITLQRTILKAWQEIDDAMADFSAAQQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRDRLKETVAQNETAVRLAQQQYVQGASDFLNVLTLQNALLASQNQLVQSTTAVSVAVAR
HHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYRALGGGWEARYPEAVARRSTIQSAGQGGRQG
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLALASALLLGACTVGPDYHKPDLASAPHWRRGMADAESHPVESPADPQWWTIYRDPVLT
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEECCHHHH
ALEQQVASANLDLRASAYRLAESIAERRIASAAQFPHMEGNASYARERASTNGILGLLGT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
LERAGAGDVASGAQGFGPVAVPGTVGNPSFNLPQYGMSASWEVDLWGHVRRQVEAANAAV
HHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
QATQDMQRDVLVSLMAETAQDYLDLRGVQAQIAIVDRNIDIARHSVDLTAMRFTQGASTQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHCCCCEEEEECCCCCCE
LDVADSRGQLSEFQSRLPILRSQEVHLINALSFLVAREPGALRAQLGTPAPIPPVPDAVP
EECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCC
VGLPSELAERRPDIRMAEEQLHVATASIGVAVADFFPRVTLSGSLDVQALQFSGLGSWAS
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEECCCCCHHH
RQYGFGPTMTLPLFEGGRLTGQLRLRRAQQKEAAITLQRTILKAWQEIDDAMADFSAAQQ
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRDRLKETVAQNETAVRLAQQQYVQGASDFLNVLTLQNALLASQNQLVQSTTAVSVAVAR
HHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYRALGGGWEARYPEAVARRSTIQSAGQGGRQG
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]