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The map label for this gene is otsA [H]

Identifier: 162146738

GI number: 162146738

Start: 929070

End: 930419

Strand: Direct

Name: otsA [H]

Synonym: GDI_0916

Alternate gene names: 162146738

Gene position: 929070-930419 (Clockwise)

Preceding gene: 162146737

Following gene: 162146739

Centisome position: 23.56

GC content: 67.33

Gene sequence:

>1350_bases
ATGGGGCGTCTGATCATCGTATCGAACCGGGTTCCATCCCCGCGCGAACGCACGCAGCCCGCCGGTGGGCTGACGGTCGG
GCTGAAGGATGCGCTGCGCGGCGGTTCGTCGCTATGGTTCGGCTGGTCGGGCAACCAGGCCGAGACCGACGCCGACCCCC
AGCCCAGCTTCGACACGGTCGAGGGCGTGACCTATGCCACCATCGACCTGACCGCCCGCCAGCACGAGGGATTCTACCAG
AATTTCTCGAACGGCATCCTCTGGCCGCTGTTTCACTACCGCGTCGGGCTGATGAACTATTCCCGCCGCGACTGGCAGAC
CTATCTGGAGGTCAACGCCCTGTTCGCGCGCTGCCTGGTGCCGCTGCTGCGCCCCGACGACGTGATCTGGGTCCATGATT
ATCACCTGTTCCCGCTGGGCCAGGCGCTGCGCGATCTGGGGGTGACGACGCGCATCGGCTTCTTCCTGCATATCCCCGTC
CCGCCCTGGAGCCTGGTGCGGTCCATGCCCGGCGCGGAACTGCTGCTGGAGGACATGCAGGCCTATGACCTGATCGGCGT
GCAGACTGACGAGGATGCCGACAACCTGAACCAGGCCTTCCGCCTGAACGGCCTGGAGGAACGGGCCCGGGCCTTTCCCA
TCGGGATCGATCCGTATGAATTCCGCGCCCAGGCCGAGGCCAGCGTCCTGCGTGACGAGGCCGGGCGCCTGCGGACCAGC
CTGCGGGGGGCCGCGCTGATCCTGGGGGTGGACCGGCTGGATTACTCCAAGGGCCTGCCCGAGCGGTTCCGGGGGTACGA
AGCGCTGATGGCGCGCTATCCCGAACATCGGGGCAAGGTGACCTTCCTGCAGGTCGCCCCGGTCTCGCGCGGCGATGTCG
AGGAATATCGCCTGCTGCGCCGGCAACTGGACGAGCTGACCGGGCGGATCAACGGCGCTTATGCCGAATTCGACTGGACG
CCCATCCGCTATATCACGCGGCCGATCGCGCGTGACATCCTGGCCGGGTTCTATCGCATGGCCGATGTCGCGCTGATCAC
GCCGCTGCGCGACGGGATGAACCTGGTGGCCAAGGAATATGTCGCGGCGCAGAACGCGGCGGCACCGGGCGTGCTGGTGT
TGTCGCACTTCGCCGGGGCGGCGCCGGAAATGGACGAGGCCGTTCTGGTCAACCCCTATGACGCCGACGAAATCGCCGAC
GCGCTGCATCAGGCGCTGACCATGAGCCTGGACGAACGCCGCCAGCGCTGGACCAAGCTGGATGAGGACGTGCGCCGGAC
CACGGCCGGATTCTGGGCCCGCGACTTCCTTCGCGCACTGGACGAGGCCCCGCCGGTCTGTCCCGTGTGA

Upstream 100 bases:

>100_bases
CCGCGCTGGGCGGCGTGGGGTTCCGCATCCCCAGGGATTTCCCCGATCCCGCCGCCTTCCGGGCCTGGCTGGCGACTCTG
GCGAAAGGGGGGAACGATTC

Downstream 100 bases:

>100_bases
TGGCATTGAGGACGGGACGCAGGATCTTCCGGGGCGGCACAGGGCGGCCGGGCGCAGGGCCCGGCATGGGCACGGCCCTG
GTTCTGGCTGCCGCCGGCGC

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGRLIIVSNRVPSPRERTQPAGGLTVGLKDALRGGSSLWFGWSGNQAETDADPQPSFDTVEGVTYATIDLTARQHEGFYQ
NFSNGILWPLFHYRVGLMNYSRRDWQTYLEVNALFARCLVPLLRPDDVIWVHDYHLFPLGQALRDLGVTTRIGFFLHIPV
PPWSLVRSMPGAELLLEDMQAYDLIGVQTDEDADNLNQAFRLNGLEERARAFPIGIDPYEFRAQAEASVLRDEAGRLRTS
LRGAALILGVDRLDYSKGLPERFRGYEALMARYPEHRGKVTFLQVAPVSRGDVEEYRLLRRQLDELTGRINGAYAEFDWT
PIRYITRPIARDILAGFYRMADVALITPLRDGMNLVAKEYVAAQNAAAPGVLVLSHFAGAAPEMDEAVLVNPYDADEIAD
ALHQALTMSLDERRQRWTKLDEDVRRTTAGFWARDFLRALDEAPPVCPV

Sequences:

>Translated_449_residues
MGRLIIVSNRVPSPRERTQPAGGLTVGLKDALRGGSSLWFGWSGNQAETDADPQPSFDTVEGVTYATIDLTARQHEGFYQ
NFSNGILWPLFHYRVGLMNYSRRDWQTYLEVNALFARCLVPLLRPDDVIWVHDYHLFPLGQALRDLGVTTRIGFFLHIPV
PPWSLVRSMPGAELLLEDMQAYDLIGVQTDEDADNLNQAFRLNGLEERARAFPIGIDPYEFRAQAEASVLRDEAGRLRTS
LRGAALILGVDRLDYSKGLPERFRGYEALMARYPEHRGKVTFLQVAPVSRGDVEEYRLLRRQLDELTGRINGAYAEFDWT
PIRYITRPIARDILAGFYRMADVALITPLRDGMNLVAKEYVAAQNAAAPGVLVLSHFAGAAPEMDEAVLVNPYDADEIAD
ALHQALTMSLDERRQRWTKLDEDVRRTTAGFWARDFLRALDEAPPVCPV
>Mature_448_residues
GRLIIVSNRVPSPRERTQPAGGLTVGLKDALRGGSSLWFGWSGNQAETDADPQPSFDTVEGVTYATIDLTARQHEGFYQN
FSNGILWPLFHYRVGLMNYSRRDWQTYLEVNALFARCLVPLLRPDDVIWVHDYHLFPLGQALRDLGVTTRIGFFLHIPVP
PWSLVRSMPGAELLLEDMQAYDLIGVQTDEDADNLNQAFRLNGLEERARAFPIGIDPYEFRAQAEASVLRDEAGRLRTSL
RGAALILGVDRLDYSKGLPERFRGYEALMARYPEHRGKVTFLQVAPVSRGDVEEYRLLRRQLDELTGRINGAYAEFDWTP
IRYITRPIARDILAGFYRMADVALITPLRDGMNLVAKEYVAAQNAAAPGVLVLSHFAGAAPEMDEAVLVNPYDADEIADA
LHQALTMSLDERRQRWTKLDEDVRRTTAGFWARDFLRALDEAPPVCPV

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=460, Percent_Identity=46.9565217391304, Blast_Score=407, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI115533558, Length=350, Percent_Identity=29.4285714285714, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI115533560, Length=350, Percent_Identity=29.1428571428571, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71987012, Length=423, Percent_Identity=27.8959810874704, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=35.6223175965665, Blast_Score=309, Evalue=6e-85,
Organism=Saccharomyces cerevisiae, GI6323537, Length=383, Percent_Identity=28.4595300261097, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320279, Length=457, Percent_Identity=26.4770240700219, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323917, Length=397, Percent_Identity=27.7078085642317, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI19920676, Length=484, Percent_Identity=38.2231404958678, Blast_Score=317, Evalue=8e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 50636; Mature: 50505

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLIIVSNRVPSPRERTQPAGGLTVGLKDALRGGSSLWFGWSGNQAETDADPQPSFDTV
CCCEEEEECCCCCCHHHCCCCCCEEEEHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHH
EGVTYATIDLTARQHEGFYQNFSNGILWPLFHYRVGLMNYSRRDWQTYLEVNALFARCLV
CCCEEEEEEEEEHHHCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PLLRPDDVIWVHDYHLFPLGQALRDLGVTTRIGFFLHIPVPPWSLVRSMPGAELLLEDMQ
HHCCCCCEEEEECEEECHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHH
AYDLIGVQTDEDADNLNQAFRLNGLEERARAFPIGIDPYEFRAQAEASVLRDEAGRLRTS
HHHEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRGAALILGVDRLDYSKGLPERFRGYEALMARYPEHRGKVTFLQVAPVSRGDVEEYRLLR
HCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHCCHHCCCEEEEEECCCCCCCHHHHHHHH
RQLDELTGRINGAYAEFDWTPIRYITRPIARDILAGFYRMADVALITPLRDGMNLVAKEY
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAQNAAAPGVLVLSHFAGAAPEMDEAVLVNPYDADEIADALHQALTMSLDERRQRWTKL
HHHCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DEDVRRTTAGFWARDFLRALDEAPPVCPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GRLIIVSNRVPSPRERTQPAGGLTVGLKDALRGGSSLWFGWSGNQAETDADPQPSFDTV
CCEEEEECCCCCCHHHCCCCCCEEEEHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHH
EGVTYATIDLTARQHEGFYQNFSNGILWPLFHYRVGLMNYSRRDWQTYLEVNALFARCLV
CCCEEEEEEEEEHHHCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
PLLRPDDVIWVHDYHLFPLGQALRDLGVTTRIGFFLHIPVPPWSLVRSMPGAELLLEDMQ
HHCCCCCEEEEECEEECHHHHHHHHCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHH
AYDLIGVQTDEDADNLNQAFRLNGLEERARAFPIGIDPYEFRAQAEASVLRDEAGRLRTS
HHHEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRGAALILGVDRLDYSKGLPERFRGYEALMARYPEHRGKVTFLQVAPVSRGDVEEYRLLR
HCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHCCHHCCCEEEEEECCCCCCCHHHHHHHH
RQLDELTGRINGAYAEFDWTPIRYITRPIARDILAGFYRMADVALITPLRDGMNLVAKEY
HHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAAQNAAAPGVLVLSHFAGAAPEMDEAVLVNPYDADEIADALHQALTMSLDERRQRWTKL
HHHCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DEDVRRTTAGFWARDFLRALDEAPPVCPV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA