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The map label for this gene is otsB [H]

Identifier: 162146737

GI number: 162146737

Start: 928324

End: 929082

Strand: Direct

Name: otsB [H]

Synonym: GDI_0915

Alternate gene names: 162146737

Gene position: 928324-929082 (Clockwise)

Preceding gene: 162146736

Following gene: 162146738

Centisome position: 23.54

GC content: 68.91

Gene sequence:

>759_bases
ATGACCCCGACGGACTATGCCGGCCCGGCCGGCCGTTTCATGCCCCCCCCGCACGAAGCCGCCCTGCTGCTGGATTTCGA
CGGCACGCTGGTCGATATCGCGCCCACGCCCGACTCGGTGGTGGTTCCGCCTGACCTGCTGGACGTGCTGCGGCAGCTGC
GGGCAAAATGCGGCGATGCGCTGGCGGTGATTTCGGGCCGGGCCGTGGACCAGATCGATCGTTTCCTGGGTGACGTGCCT
TTCGCCGTCGCGGGTGAACACGGGATCGCGGTACGGCATCGGCCGGACGGACCGATCGTGCGCGCCGACCTGCCGCATGT
GCCCTCGACCTGGCTGCAGGCGGCGGAAACCCTGGCCGCGGCGCATCCGGGCGTACGGATCGAAACCAAGGTCGCGGGGT
TCGTCCTGCATTATCGCGCGGCGCCCGAAACGGCCGACCTGCTGCGCGAGGCAGCAGAAACCTGGGTCGCACCCAGCAAT
GGGGCGTTCCATATCCAGGCCGCCAAGATGGCATGGGAAATCCGTCCCCACGGCGTGGACAAGGGCCATGCGGTCGCGGA
ACTGATGCAGTCGCCGCCCTTTGCCGGCCGGCATCCGATCTTCATCGGTGACGACGTGACGGACGAGGACGGCATTCGCC
AGGCCGCCGCGCTGGGCGGCGTGGGGTTCCGCATCCCCAGGGATTTCCCCGATCCCGCCGCCTTCCGGGCCTGGCTGGCG
ACTCTGGCGAAAGGGGGGAACGATTCATGGGGCGTCTGA

Upstream 100 bases:

>100_bases
CCAATCCCTGCTTCCGCCCTGATGGCGGAGGCAGCAACCTCGGTCCGCGCGGCCGGTTCGGGATGAATGCCGTCCCATCT
ACGCAAGGAGTGAGACGCCC

Downstream 100 bases:

>100_bases
TCATCGTATCGAACCGGGTTCCATCCCCGCGCGAACGCACGCAGCCCGCCGGTGGGCTGACGGTCGGGCTGAAGGATGCG
CTGCGCGGCGGTTCGTCGCT

Product: trehalose-phosphatase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTPTDYAGPAGRFMPPPHEAALLLDFDGTLVDIAPTPDSVVVPPDLLDVLRQLRAKCGDALAVISGRAVDQIDRFLGDVP
FAVAGEHGIAVRHRPDGPIVRADLPHVPSTWLQAAETLAAAHPGVRIETKVAGFVLHYRAAPETADLLREAAETWVAPSN
GAFHIQAAKMAWEIRPHGVDKGHAVAELMQSPPFAGRHPIFIGDDVTDEDGIRQAAALGGVGFRIPRDFPDPAAFRAWLA
TLAKGGNDSWGV

Sequences:

>Translated_252_residues
MTPTDYAGPAGRFMPPPHEAALLLDFDGTLVDIAPTPDSVVVPPDLLDVLRQLRAKCGDALAVISGRAVDQIDRFLGDVP
FAVAGEHGIAVRHRPDGPIVRADLPHVPSTWLQAAETLAAAHPGVRIETKVAGFVLHYRAAPETADLLREAAETWVAPSN
GAFHIQAAKMAWEIRPHGVDKGHAVAELMQSPPFAGRHPIFIGDDVTDEDGIRQAAALGGVGFRIPRDFPDPAAFRAWLA
TLAKGGNDSWGV
>Mature_251_residues
TPTDYAGPAGRFMPPPHEAALLLDFDGTLVDIAPTPDSVVVPPDLLDVLRQLRAKCGDALAVISGRAVDQIDRFLGDVPF
AVAGEHGIAVRHRPDGPIVRADLPHVPSTWLQAAETLAAAHPGVRIETKVAGFVLHYRAAPETADLLREAAETWVAPSNG
AFHIQAAKMAWEIRPHGVDKGHAVAELMQSPPFAGRHPIFIGDDVTDEDGIRQAAALGGVGFRIPRDFPDPAAFRAWLAT
LAKGGNDSWGV

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=208, Percent_Identity=39.4230769230769, Blast_Score=130, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19920676, Length=230, Percent_Identity=31.7391304347826, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221473368, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129307, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24582490, Length=233, Percent_Identity=28.3261802575107, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26834; Mature: 26702

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTDYAGPAGRFMPPPHEAALLLDFDGTLVDIAPTPDSVVVPPDLLDVLRQLRAKCGDA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCE
LAVISGRAVDQIDRFLGDVPFAVAGEHGIAVRHRPDGPIVRADLPHVPSTWLQAAETLAA
EEECCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
AHPGVRIETKVAGFVLHYRAAPETADLLREAAETWVAPSNGAFHIQAAKMAWEIRPHGVD
CCCCCEEEEEHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCC
KGHAVAELMQSPPFAGRHPIFIGDDVTDEDGIRQAAALGGVGFRIPRDFPDPAAFRAWLA
CHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
TLAKGGNDSWGV
HHHCCCCCCCCC
>Mature Secondary Structure 
TPTDYAGPAGRFMPPPHEAALLLDFDGTLVDIAPTPDSVVVPPDLLDVLRQLRAKCGDA
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEECHHHHHHHHHHHHHHCCE
LAVISGRAVDQIDRFLGDVPFAVAGEHGIAVRHRPDGPIVRADLPHVPSTWLQAAETLAA
EEECCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHH
AHPGVRIETKVAGFVLHYRAAPETADLLREAAETWVAPSNGAFHIQAAKMAWEIRPHGVD
CCCCCEEEEEHEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCC
KGHAVAELMQSPPFAGRHPIFIGDDVTDEDGIRQAAALGGVGFRIPRDFPDPAAFRAWLA
CHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
TLAKGGNDSWGV
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]