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The map label for this gene is ahpF [H]

Identifier: 162146595

GI number: 162146595

Start: 790076

End: 791680

Strand: Reverse

Name: ahpF [H]

Synonym: GDI_0773

Alternate gene names: 162146595

Gene position: 791680-790076 (Counterclockwise)

Preceding gene: 162146596

Following gene: 162146588

Centisome position: 20.07

GC content: 64.86

Gene sequence:

>1605_bases
ATGCGGCCGGGCAACCGCCCCACTTCACTGGAATACGGAATCCTCCCGATGCTGCAGGATACGATCAAGTCACAACTCAA
GGACCATTTCGCCAATCTTGCACATCCGATCGTGCTCGAAGCCTCGCTCGACGACAGCGCTCCGGCCCGGGACATGGCGG
CATTGCTGCACGAGATCGCCGCGCTTTCCGGCAAGATTCGCGTCTCGACGGACGGCGTGGCGTCGCGGCGCCCATCCTTC
GTAATTCGGCGCGAGGACGCAGCGGGCAGCGTGGCGTTCGCCGCCATTCCCATGGGACATGAATTCACCTCGCTGGTTCT
GGCGCTGTTGCAGGTCGGGGGTCATGCGCCGAAACTGCCGGACGACGTTACGGCCCAGATCAGGTCGCTGAGCGGCACGT
ATCGTTTCGAAACCTATGTTTCGCTGTCCTGCCAGAACTGCCCGGATGTCGTGCAGGCGCTCAATGCGATGAGCGTGCTG
AACCCGAACATCAGCAACATCACGATCGACGGCGCGCTGTACCAGCGTGAGGTCGAAGACAAGAACATCATGTCCGTCCC
CCAGGTGTACCTGAACGGACAGCCCTTCGGGTCCGGCCGCATGGGTGTCGCGGAGATCCTGGCGAAGCTGGATGCCGACG
CACCGAAACGGATCGCGGAACGGCTGAAGGATCTTGCCCCGTTCGACATCCTGGTCGTGGGCGCGGGTCCGGCCGGGTCG
GCGGCGGCGGTCTATGCGGCGCGCAAGGGGCTGCGTACCGGCCTGATCGCCGAGCGGTTCGGCGGCCAGGTGATGGACAC
CGCCGGCATCGAGAATTTCATCTCGGTCAGGGAAACGGAGGGGCCGAAGCTGGCCGCCGCGCTGGAGGCGCATGTGCGCC
AATACGACGTGGAGGTCATTTCCGCGCAACGGGTGCGGGAGTTGCATGCCGCAGCACAGCCCGGCGCCCTCCACCACGTC
GTGCTGGAGAGCGGGGCGAGCCTGCGGGCGCGCACGATCATTCTTGCAACCGGAGCGCACTGGAGACATCTCAACGTCCC
CGGCGAGGAGGAGTATCGCACCCGTGGGGTGGCCTACTGCCCGCACTGTGACGGTCCTTTCTTCAAGGGAAAGCCGGTCG
CGGTCGCCGGAGGGGGCAATAGCGGCGTCGAAGCCGCGATCGACCTCGCCGGGATCAGTTCCCACGTGACGCTGCTGGAG
CGCGACCCGGCGCTGCGGGCGGACAGGCTCCTGCAGGACAAGCTTCGCAGCCTGTCCAATGTCAGCATCATGACGAATGC
CGTGATCACTAAGGTCGAAGGCAACGGCACGAAGATGACGGGCCTGACCGTTCAGGACGGCAGCGGAACCGCGCGGTATC
TGGACGTCGACGGCGTATTCGTCCAGATCGGACTGCTTCCCAATACCGATTGGCTTCGCGGCACGATCGAGCGTACGGCG
TTCGGCGAAATTATCGTCGATGGCCGGGGCATGACGTCGATTCCCGGAATCTTCGCGGCGGGCGATGCGACGACCACGCC
GTACAAGCAGATCGTCATCGCGATCGGGGACGGGGCCAAGGCCGCGCTGGCCGCGTTCGACTACCTTATCCGCCTGCCCG
GCTGA

Upstream 100 bases:

>100_bases
CGGCGAAGTCTGCCCCGCCAAGTGGAAGGAGGACGGCGCGACCCTGACCCCGTCCCTGGATCTGATCGGCAAGATCTGAC
CCCAGCCACAGGCCCGGCCC

Downstream 100 bases:

>100_bases
CCGCCTGGTCCAGAACGTCGTCGGCCGTGACCGAACGTGACGAGGTGGAATCGCCTCGTCACGATCCATCCTTCTTATCC
GGGGAGTTTGACGGTGACAC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MRPGNRPTSLEYGILPMLQDTIKSQLKDHFANLAHPIVLEASLDDSAPARDMAALLHEIAALSGKIRVSTDGVASRRPSF
VIRREDAAGSVAFAAIPMGHEFTSLVLALLQVGGHAPKLPDDVTAQIRSLSGTYRFETYVSLSCQNCPDVVQALNAMSVL
NPNISNITIDGALYQREVEDKNIMSVPQVYLNGQPFGSGRMGVAEILAKLDADAPKRIAERLKDLAPFDILVVGAGPAGS
AAAVYAARKGLRTGLIAERFGGQVMDTAGIENFISVRETEGPKLAAALEAHVRQYDVEVISAQRVRELHAAAQPGALHHV
VLESGASLRARTIILATGAHWRHLNVPGEEEYRTRGVAYCPHCDGPFFKGKPVAVAGGGNSGVEAAIDLAGISSHVTLLE
RDPALRADRLLQDKLRSLSNVSIMTNAVITKVEGNGTKMTGLTVQDGSGTARYLDVDGVFVQIGLLPNTDWLRGTIERTA
FGEIIVDGRGMTSIPGIFAAGDATTTPYKQIVIAIGDGAKAALAAFDYLIRLPG

Sequences:

>Translated_534_residues
MRPGNRPTSLEYGILPMLQDTIKSQLKDHFANLAHPIVLEASLDDSAPARDMAALLHEIAALSGKIRVSTDGVASRRPSF
VIRREDAAGSVAFAAIPMGHEFTSLVLALLQVGGHAPKLPDDVTAQIRSLSGTYRFETYVSLSCQNCPDVVQALNAMSVL
NPNISNITIDGALYQREVEDKNIMSVPQVYLNGQPFGSGRMGVAEILAKLDADAPKRIAERLKDLAPFDILVVGAGPAGS
AAAVYAARKGLRTGLIAERFGGQVMDTAGIENFISVRETEGPKLAAALEAHVRQYDVEVISAQRVRELHAAAQPGALHHV
VLESGASLRARTIILATGAHWRHLNVPGEEEYRTRGVAYCPHCDGPFFKGKPVAVAGGGNSGVEAAIDLAGISSHVTLLE
RDPALRADRLLQDKLRSLSNVSIMTNAVITKVEGNGTKMTGLTVQDGSGTARYLDVDGVFVQIGLLPNTDWLRGTIERTA
FGEIIVDGRGMTSIPGIFAAGDATTTPYKQIVIAIGDGAKAALAAFDYLIRLPG
>Mature_534_residues
MRPGNRPTSLEYGILPMLQDTIKSQLKDHFANLAHPIVLEASLDDSAPARDMAALLHEIAALSGKIRVSTDGVASRRPSF
VIRREDAAGSVAFAAIPMGHEFTSLVLALLQVGGHAPKLPDDVTAQIRSLSGTYRFETYVSLSCQNCPDVVQALNAMSVL
NPNISNITIDGALYQREVEDKNIMSVPQVYLNGQPFGSGRMGVAEILAKLDADAPKRIAERLKDLAPFDILVVGAGPAGS
AAAVYAARKGLRTGLIAERFGGQVMDTAGIENFISVRETEGPKLAAALEAHVRQYDVEVISAQRVRELHAAAQPGALHHV
VLESGASLRARTIILATGAHWRHLNVPGEEEYRTRGVAYCPHCDGPFFKGKPVAVAGGGNSGVEAAIDLAGISSHVTLLE
RDPALRADRLLQDKLRSLSNVSIMTNAVITKVEGNGTKMTGLTVQDGSGTARYLDVDGVFVQIGLLPNTDWLRGTIERTA
FGEIIVDGRGMTSIPGIFAAGDATTTPYKQIVIAIGDGAKAALAAFDYLIRLPG

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=57.8336557059961, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787114, Length=312, Percent_Identity=35.8974358974359, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6321898, Length=311, Percent_Identity=33.7620578778135, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=33.1210191082803, Blast_Score=160, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56745; Mature: 56745

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGNRPTSLEYGILPMLQDTIKSQLKDHFANLAHPIVLEASLDDSAPARDMAALLHEIA
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
ALSGKIRVSTDGVASRRPSFVIRREDAAGSVAFAAIPMGHEFTSLVLALLQVGGHAPKLP
HHCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
DDVTAQIRSLSGTYRFETYVSLSCQNCPDVVQALNAMSVLNPNISNITIDGALYQREVED
HHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCC
KNIMSVPQVYLNGQPFGSGRMGVAEILAKLDADAPKRIAERLKDLAPFDILVVGAGPAGS
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
AAAVYAARKGLRTGLIAERFGGQVMDTAGIENFISVRETEGPKLAAALEAHVRQYDVEVI
HHHHHHHHHCCHHHHHHHHHCCCEEEHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SAQRVRELHAAAQPGALHHVVLESGASLRARTIILATGAHWRHLNVPGEEEYRTRGVAYC
HHHHHHHHHHHCCCCCEEEHHHHCCCCCEEEEEEEEECCCEEECCCCCCHHHHHCCEEEC
PHCDGPFFKGKPVAVAGGGNSGVEAAIDLAGISSHVTLLERDPALRADRLLQDKLRSLSN
CCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCC
VSIMTNAVITKVEGNGTKMTGLTVQDGSGTARYLDVDGVFVQIGLLPNTDWLRGTIERTA
CEEEEEEEEEEEECCCEEEEEEEEECCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHCC
FGEIIVDGRGMTSIPGIFAAGDATTTPYKQIVIAIGDGAKAALAAFDYLIRLPG
CCEEEECCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPGNRPTSLEYGILPMLQDTIKSQLKDHFANLAHPIVLEASLDDSAPARDMAALLHEIA
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHH
ALSGKIRVSTDGVASRRPSFVIRREDAAGSVAFAAIPMGHEFTSLVLALLQVGGHAPKLP
HHCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
DDVTAQIRSLSGTYRFETYVSLSCQNCPDVVQALNAMSVLNPNISNITIDGALYQREVED
HHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCC
KNIMSVPQVYLNGQPFGSGRMGVAEILAKLDADAPKRIAERLKDLAPFDILVVGAGPAGS
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
AAAVYAARKGLRTGLIAERFGGQVMDTAGIENFISVRETEGPKLAAALEAHVRQYDVEVI
HHHHHHHHHCCHHHHHHHHHCCCEEEHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SAQRVRELHAAAQPGALHHVVLESGASLRARTIILATGAHWRHLNVPGEEEYRTRGVAYC
HHHHHHHHHHHCCCCCEEEHHHHCCCCCEEEEEEEEECCCEEECCCCCCHHHHHCCEEEC
PHCDGPFFKGKPVAVAGGGNSGVEAAIDLAGISSHVTLLERDPALRADRLLQDKLRSLSN
CCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCC
VSIMTNAVITKVEGNGTKMTGLTVQDGSGTARYLDVDGVFVQIGLLPNTDWLRGTIERTA
CEEEEEEEEEEEECCCEEEEEEEEECCCCCEEEEEECCEEEEEEECCCCHHHHHHHHHCC
FGEIIVDGRGMTSIPGIFAAGDATTTPYKQIVIAIGDGAKAALAAFDYLIRLPG
CCEEEECCCCCCCCCCEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]