Click here to switch to the map view.

The map label for this gene is btuB [H]

Identifier: 162146491

GI number: 162146491

Start: 674424

End: 676520

Strand: Reverse

Name: btuB [H]

Synonym: GDI_0667

Alternate gene names: 162146491

Gene position: 676520-674424 (Counterclockwise)

Preceding gene: 162146492

Following gene: 162146490

Centisome position: 17.15

GC content: 60.75

Gene sequence:

>2097_bases
ATGGGAACCTGCGTGGCGCAGGCTGACGAGCCCGCGAAGCAAGCTGCCCCGGCCAAGCGATCGAAGCCACATGTTGTCCA
GGGCGGCGACCAGGCCGGCAAGTCGAAGCCCGCGACCACGTCATCGAACGTCCCGGCCCGGACATCCAGCCCCGCCGTTT
CCACGCAGACCGCCGAACTGCCCGAACTGATCAGTGTCAGTGCCGCGCGCCGGCCGACCTCGGTCGACGATATCGGCACC
AGCATGACGATCGTGACCGAACAGCAGATCCAGACCCAGCAGCGCCGGGGACTGGCCGATATCCTGGAGCGCCAGCCGGG
CCTGAACGTCGTGCAGACGGGTGGCCCCGGGGGGACGACATCCATTTTCATGCGCGGCAACAACGCGAACGAGGTCAAGG
TCCGTCTGGACGGCATGGACGTCAATGACGGCAGTTCCGCCCAGGGTATGTTCGACGCCGCCCAGTTCATGACGGACGGC
ATGGGACGGATCGAGATCCTGCGCGGCCCGCAAAGCGGCCTGTATGGCGCGGATGCCATGGCAGGCGTGATCGATATCAC
CACGGCGCAGGGGGAAGGCCGCTTCAAGCCCTTCGTCCGGATCGAAGGCGGCGGATACGGCACCTTCAACCAGGTCTTCG
GCGCGCGCGGCAGCAAGGGGCGCTTCCATTACAACGTTGAATTTTCCCATTACCGAATGGACGGATTCAACAACATCCCG
TCTTATATCGAGCGCGCCTACAACGCCGACTGGCGGCAGCGCCACGAAGGCAATCGCAACGACAACCGCAGCGCCAATAT
CCGCCTGGGGTATGATGTTGCCCGCAATTTCGACCTGGGCCTGACGACCCGCCTGATACAGGGAAACAACCACAGCTATT
CCCTCTATGACCTGCAAAACGAGTATTTCGGATATTCCGGCGTGCGCGAACAAAACAACACGAACGAGGCGATCGTGCGG
GGTACGGCGCATCATGTCTCGTTCAACGGGCTGTTCGACCAGGTGATCGGCCTGGGATACATGACCAACCGCAATCGCTG
GACGGAGACGGGAACGAGCTATGGCAGCCCGATCAACCCCGATCCCGATTACTACCGTTCCGACCGGTTGAAGATCGACT
GGCACGGCACGTTCAATTTCCGGCAGTACGGTCAGTTCATGGTCGGCGCCGAACATTTCCACGAGACATTCAATACCAGC
CAGGCCGGCGGCATTTCTGGTCCCGGATATGACACGTCCGCCGGCATGGACACGACCGCCGGATACGGGCAGTACCAGGG
AAACTGGAACCACATCCTGTATGGCGCCGCCAATATCCGGTATGATGCGAATTCACGATACGGCAACCATGTGACCTGGC
GCGTGGCGCCCGCCATCCATGTTCCGGGCACCGGCACCATCATCAAGGCCAGCGCCGGTTCCGGATTTCATGGACCGTCC
CTGTACCAGCTCTTCGCCAACCAGGGCGGGTACGGGTATTCCGAAAAGGGCAATCCGAACCTGAAGGCCGAACAACTGAT
CGGCTATGACGCCGGGGTCGAGCAGAAGCTGCTGCATGATAGCCTGAATTTCGGCGCGACCTGGTATGACAACCGGGTCC
GGAACCTGATCCAGTCGACCCTGTCCATTGATTCCTCCTATAACTACAACTATTCGTACGCCAACGTGGACCGGGCGCGC
ATGTACGGCGTGGAGGCCTTCCTGAACTGGAAGGCACGCCGCGACCTGCAGTTCAACCTGAGCTACACCTGGACCCATGC
GCGCAACGAGACGGACGATACGGAACTGCAGCGCCGTCCCCAGCATAAATTCAACTTCAACACGACATGGACGCCCATCC
AGGACCTGACCTTTTCGGGAAACATCCTGTACACCAGCGCCTGGCGCGACCTGCCGGTCATCGGCAGCGAATCATGCAGC
GTATGCGCCAAGGGGCATGGCTATTTTACGATCGATATCGCCGCCAATTACAAGATCAACAGATATGTCTCGGTCTATGC
GCGCGCGGACAACCTGCTGAACAGGCAATATGAGGACCCGATCGGCTACCTGCAGCCCGGGCGCGCGGGCTATGGCGGCT
TTACGCTGAATTACTGA

Upstream 100 bases:

>100_bases
ATGACAGTACGCTCTCGTGGCAACGATGATGTGACCGCGAGACCTGGACCTGATGAGTGTTTTCCGTAATCTGTTGATCG
CTTCGAGCACGTTCGTGGCG

Downstream 100 bases:

>100_bases
CATGAAACGGGCGCTCTGGGGCATGGTGGCGGGTTTGCTGGCATGGACCAGCGCGGGCCAGGGCCGGCCGTTGCCCCGGG
TGGCGTCCCTGAACCTCTGC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MGTCVAQADEPAKQAAPAKRSKPHVVQGGDQAGKSKPATTSSNVPARTSSPAVSTQTAELPELISVSAARRPTSVDDIGT
SMTIVTEQQIQTQQRRGLADILERQPGLNVVQTGGPGGTTSIFMRGNNANEVKVRLDGMDVNDGSSAQGMFDAAQFMTDG
MGRIEILRGPQSGLYGADAMAGVIDITTAQGEGRFKPFVRIEGGGYGTFNQVFGARGSKGRFHYNVEFSHYRMDGFNNIP
SYIERAYNADWRQRHEGNRNDNRSANIRLGYDVARNFDLGLTTRLIQGNNHSYSLYDLQNEYFGYSGVREQNNTNEAIVR
GTAHHVSFNGLFDQVIGLGYMTNRNRWTETGTSYGSPINPDPDYYRSDRLKIDWHGTFNFRQYGQFMVGAEHFHETFNTS
QAGGISGPGYDTSAGMDTTAGYGQYQGNWNHILYGAANIRYDANSRYGNHVTWRVAPAIHVPGTGTIIKASAGSGFHGPS
LYQLFANQGGYGYSEKGNPNLKAEQLIGYDAGVEQKLLHDSLNFGATWYDNRVRNLIQSTLSIDSSYNYNYSYANVDRAR
MYGVEAFLNWKARRDLQFNLSYTWTHARNETDDTELQRRPQHKFNFNTTWTPIQDLTFSGNILYTSAWRDLPVIGSESCS
VCAKGHGYFTIDIAANYKINRYVSVYARADNLLNRQYEDPIGYLQPGRAGYGGFTLNY

Sequences:

>Translated_698_residues
MGTCVAQADEPAKQAAPAKRSKPHVVQGGDQAGKSKPATTSSNVPARTSSPAVSTQTAELPELISVSAARRPTSVDDIGT
SMTIVTEQQIQTQQRRGLADILERQPGLNVVQTGGPGGTTSIFMRGNNANEVKVRLDGMDVNDGSSAQGMFDAAQFMTDG
MGRIEILRGPQSGLYGADAMAGVIDITTAQGEGRFKPFVRIEGGGYGTFNQVFGARGSKGRFHYNVEFSHYRMDGFNNIP
SYIERAYNADWRQRHEGNRNDNRSANIRLGYDVARNFDLGLTTRLIQGNNHSYSLYDLQNEYFGYSGVREQNNTNEAIVR
GTAHHVSFNGLFDQVIGLGYMTNRNRWTETGTSYGSPINPDPDYYRSDRLKIDWHGTFNFRQYGQFMVGAEHFHETFNTS
QAGGISGPGYDTSAGMDTTAGYGQYQGNWNHILYGAANIRYDANSRYGNHVTWRVAPAIHVPGTGTIIKASAGSGFHGPS
LYQLFANQGGYGYSEKGNPNLKAEQLIGYDAGVEQKLLHDSLNFGATWYDNRVRNLIQSTLSIDSSYNYNYSYANVDRAR
MYGVEAFLNWKARRDLQFNLSYTWTHARNETDDTELQRRPQHKFNFNTTWTPIQDLTFSGNILYTSAWRDLPVIGSESCS
VCAKGHGYFTIDIAANYKINRYVSVYARADNLLNRQYEDPIGYLQPGRAGYGGFTLNY
>Mature_697_residues
GTCVAQADEPAKQAAPAKRSKPHVVQGGDQAGKSKPATTSSNVPARTSSPAVSTQTAELPELISVSAARRPTSVDDIGTS
MTIVTEQQIQTQQRRGLADILERQPGLNVVQTGGPGGTTSIFMRGNNANEVKVRLDGMDVNDGSSAQGMFDAAQFMTDGM
GRIEILRGPQSGLYGADAMAGVIDITTAQGEGRFKPFVRIEGGGYGTFNQVFGARGSKGRFHYNVEFSHYRMDGFNNIPS
YIERAYNADWRQRHEGNRNDNRSANIRLGYDVARNFDLGLTTRLIQGNNHSYSLYDLQNEYFGYSGVREQNNTNEAIVRG
TAHHVSFNGLFDQVIGLGYMTNRNRWTETGTSYGSPINPDPDYYRSDRLKIDWHGTFNFRQYGQFMVGAEHFHETFNTSQ
AGGISGPGYDTSAGMDTTAGYGQYQGNWNHILYGAANIRYDANSRYGNHVTWRVAPAIHVPGTGTIIKASAGSGFHGPSL
YQLFANQGGYGYSEKGNPNLKAEQLIGYDAGVEQKLLHDSLNFGATWYDNRVRNLIQSTLSIDSSYNYNYSYANVDRARM
YGVEAFLNWKARRDLQFNLSYTWTHARNETDDTELQRRPQHKFNFNTTWTPIQDLTFSGNILYTSAWRDLPVIGSESCSV
CAKGHGYFTIDIAANYKINRYVSVYARADNLLNRQYEDPIGYLQPGRAGYGGFTLNY

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=667, Percent_Identity=23.2383808095952, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790405, Length=132, Percent_Identity=37.8787878787879, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786798, Length=264, Percent_Identity=25, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 77456; Mature: 77325

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTCVAQADEPAKQAAPAKRSKPHVVQGGDQAGKSKPATTSSNVPARTSSPAVSTQTAEL
CCCCCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PELISVSAARRPTSVDDIGTSMTIVTEQQIQTQQRRGLADILERQPGLNVVQTGGPGGTT
HHHHHHHHCCCCCCHHHCCCEEEEEEHHHHHHHHHCCHHHHHHCCCCCCEEEECCCCCCE
SIFMRGNNANEVKVRLDGMDVNDGSSAQGMFDAAQFMTDGMGRIEILRGPQSGLYGADAM
EEEEECCCCCEEEEEEECEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
AGVIDITTAQGEGRFKPFVRIEGGGYGTFNQVFGARGSKGRFHYNVEFSHYRMDGFNNIP
CCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCEEEEEEEEEEEEECCCCCHH
SYIERAYNADWRQRHEGNRNDNRSANIRLGYDVARNFDLGLTTRLIQGNNHSYSLYDLQN
HHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCEEEEEEECCCCCEEEEECCH
EYFGYSGVREQNNTNEAIVRGTAHHVSFNGLFDQVIGLGYMTNRNRWTETGTSYGSPINP
HHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
DPDYYRSDRLKIDWHGTFNFRQYGQFMVGAEHFHETFNTSQAGGISGPGYDTSAGMDTTA
CCCCCCCCEEEEEEECCCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GYGQYQGNWNHILYGAANIRYDANSRYGNHVTWRVAPAIHVPGTGTIIKASAGSGFHGPS
CCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCH
LYQLFANQGGYGYSEKGNPNLKAEQLIGYDAGVEQKLLHDSLNFGATWYDNRVRNLIQST
HHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
LSIDSSYNYNYSYANVDRARMYGVEAFLNWKARRDLQFNLSYTWTHARNETDDTELQRRP
HCCCCCCCCCEEECCCCHHHHHCCHHEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHCC
QHKFNFNTTWTPIQDLTFSGNILYTSAWRDLPVIGSESCSVCAKGHGYFTIDIAANYKIN
CCCCCCCCCCCCHHHEEECCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEEEECCEEE
RYVSVYARADNLLNRQYEDPIGYLQPGRAGYGGFTLNY
EEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
GTCVAQADEPAKQAAPAKRSKPHVVQGGDQAGKSKPATTSSNVPARTSSPAVSTQTAEL
CCCCCCCCCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PELISVSAARRPTSVDDIGTSMTIVTEQQIQTQQRRGLADILERQPGLNVVQTGGPGGTT
HHHHHHHHCCCCCCHHHCCCEEEEEEHHHHHHHHHCCHHHHHHCCCCCCEEEECCCCCCE
SIFMRGNNANEVKVRLDGMDVNDGSSAQGMFDAAQFMTDGMGRIEILRGPQSGLYGADAM
EEEEECCCCCEEEEEEECEECCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHH
AGVIDITTAQGEGRFKPFVRIEGGGYGTFNQVFGARGSKGRFHYNVEFSHYRMDGFNNIP
CCEEEEEECCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCEEEEEEEEEEEEECCCCCHH
SYIERAYNADWRQRHEGNRNDNRSANIRLGYDVARNFDLGLTTRLIQGNNHSYSLYDLQN
HHHHHHHCCCHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCEEEEEEECCCCCEEEEECCH
EYFGYSGVREQNNTNEAIVRGTAHHVSFNGLFDQVIGLGYMTNRNRWTETGTSYGSPINP
HHCCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
DPDYYRSDRLKIDWHGTFNFRQYGQFMVGAEHFHETFNTSQAGGISGPGYDTSAGMDTTA
CCCCCCCCEEEEEEECCCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GYGQYQGNWNHILYGAANIRYDANSRYGNHVTWRVAPAIHVPGTGTIIKASAGSGFHGPS
CCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEEEECCCCCEEEEECCCCCCCCCH
LYQLFANQGGYGYSEKGNPNLKAEQLIGYDAGVEQKLLHDSLNFGATWYDNRVRNLIQST
HHHHHHCCCCCCCCCCCCCCCCHHHEECCCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
LSIDSSYNYNYSYANVDRARMYGVEAFLNWKARRDLQFNLSYTWTHARNETDDTELQRRP
HCCCCCCCCCEEECCCCHHHHHCCHHEECCCCCCCEEEEEEEEEEECCCCCCHHHHHHCC
QHKFNFNTTWTPIQDLTFSGNILYTSAWRDLPVIGSESCSVCAKGHGYFTIDIAANYKIN
CCCCCCCCCCCCHHHEEECCCEEEEECCCCCCCCCCCCCCEEECCCCEEEEEEEECCEEE
RYVSVYARADNLLNRQYEDPIGYLQPGRAGYGGFTLNY
EEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]