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The map label for this gene is cobD [H]

Identifier: 162146479

GI number: 162146479

Start: 663887

End: 664873

Strand: Direct

Name: cobD [H]

Synonym: GDI_0655

Alternate gene names: 162146479

Gene position: 663887-664873 (Clockwise)

Preceding gene: 162146478

Following gene: 162146480

Centisome position: 16.83

GC content: 73.96

Gene sequence:

>987_bases
ATGCCGGATGCTTCCTTGTCCGCGACGCTCAGCGTGGCCGCGCTGGCGGCGGTGATCGAATGGCGCGTCGGCTATCCCGC
CCCGGTCTTCGCATGGATCGGCCACCCGGTGATGTGGATCGGCGCGCTGATCGGCCGGCTGGACCGCCTGGGCAATCGGC
CCGGCTGGTCGTCGCCCGGCTGCCGGGCGGCGGGGTTCGCGGCCCTGGCGATCGTGGTCGCGGTGCCGGTCGGCCTGGCC
TGTGGGGCGGTATGGCTGATGGACCGCGTGCTGCCGCGCGGCCTTGCGATCCTGCTGCAGGCGGTGGCCGCCGGCACCCT
GGTCGCCCAGCGCTCGCTGCACGACCATGTGGCCGACGTGGGCACGGGGTTGCGCGGGGGCGGGCTGGCGGGTGGCCGGG
CGGCGGTGGGACGCATCGTGGGGCGCGACACGGCGGGGCTGGACCGCGCGGGCATCGTGCGGGCCGCGATCGAAAGCCTG
GCGGAAAATTTCTCGGACGGCGTGGTGGCGCCGCTGCTCTGGACGGCGCTGCTGGGCCTGCCGGGCGCGGTCTTCTACAA
GGCCGTCAATACCGCCGACAGCATGATCGGGCACCTGACGCCCCGCCACGCCGCCTTCGGCTTCGCCGCCGCGCGGCTGG
ACGACGGGATCAACCTGCCGGCTTCGCGCCTGTCGGCGCTGTGGATTTTCCTGGCGGCGCCGCGCGCGCGGCGGGGGGCC
GTCTGGCGGGCCCTTCGGGACGACGCGCGGCACCATCGCTCGCCCAATGCCGGCTGGCCCGAAGCCGCGATGGCCGGCGC
GCTGGGGCTGCGCCTGGCCGGTCCGCGCCTCTATGGCGGCGTGCGGGTGCAGGATGCCTGGATGGGCGACGGCACGCCGG
ATGCGGATATCGGCCATCTGGACCGTGCGCTGGTCCTCTATCGCCGGGCCTGCCTGATCGAACGGATCGTCATCGTGGCC
CTGGCGGTGTACATAACGGTCCTGTGA

Upstream 100 bases:

>100_bases
GCCATCTCGCGCGGGTTCGCGGCCCTGTCATGGCTGGTCGCCCTGGTCATCCTGGCGCTGAACGGCAAGCTGCTGTGGGA
CATGGCGACGGGCTGATCCG

Downstream 100 bases:

>100_bases
TGACCGAACCCGATCCCCGCCCCGCGTTTTCCGACGATTTCCGCGCCCAGCTCGACCGCCTGTTCGTCTGGCGGCGCGAC
GTGCGGCATTTCCGGACCGA

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPDASLSATLSVAALAAVIEWRVGYPAPVFAWIGHPVMWIGALIGRLDRLGNRPGWSSPGCRAAGFAALAIVVAVPVGLA
CGAVWLMDRVLPRGLAILLQAVAAGTLVAQRSLHDHVADVGTGLRGGGLAGGRAAVGRIVGRDTAGLDRAGIVRAAIESL
AENFSDGVVAPLLWTALLGLPGAVFYKAVNTADSMIGHLTPRHAAFGFAAARLDDGINLPASRLSALWIFLAAPRARRGA
VWRALRDDARHHRSPNAGWPEAAMAGALGLRLAGPRLYGGVRVQDAWMGDGTPDADIGHLDRALVLYRRACLIERIVIVA
LAVYITVL

Sequences:

>Translated_328_residues
MPDASLSATLSVAALAAVIEWRVGYPAPVFAWIGHPVMWIGALIGRLDRLGNRPGWSSPGCRAAGFAALAIVVAVPVGLA
CGAVWLMDRVLPRGLAILLQAVAAGTLVAQRSLHDHVADVGTGLRGGGLAGGRAAVGRIVGRDTAGLDRAGIVRAAIESL
AENFSDGVVAPLLWTALLGLPGAVFYKAVNTADSMIGHLTPRHAAFGFAAARLDDGINLPASRLSALWIFLAAPRARRGA
VWRALRDDARHHRSPNAGWPEAAMAGALGLRLAGPRLYGGVRVQDAWMGDGTPDADIGHLDRALVLYRRACLIERIVIVA
LAVYITVL
>Mature_327_residues
PDASLSATLSVAALAAVIEWRVGYPAPVFAWIGHPVMWIGALIGRLDRLGNRPGWSSPGCRAAGFAALAIVVAVPVGLAC
GAVWLMDRVLPRGLAILLQAVAAGTLVAQRSLHDHVADVGTGLRGGGLAGGRAAVGRIVGRDTAGLDRAGIVRAAIESLA
ENFSDGVVAPLLWTALLGLPGAVFYKAVNTADSMIGHLTPRHAAFGFAAARLDDGINLPASRLSALWIFLAAPRARRGAV
WRALRDDARHHRSPNAGWPEAAMAGALGLRLAGPRLYGGVRVQDAWMGDGTPDADIGHLDRALVLYRRACLIERIVIVAL
AVYITVL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34266; Mature: 34135

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDASLSATLSVAALAAVIEWRVGYPAPVFAWIGHPVMWIGALIGRLDRLGNRPGWSSPG
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
CRAAGFAALAIVVAVPVGLACGAVWLMDRVLPRGLAILLQAVAAGTLVAQRSLHDHVADV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGLRGGGLAGGRAAVGRIVGRDTAGLDRAGIVRAAIESLAENFSDGVVAPLLWTALLGL
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
PGAVFYKAVNTADSMIGHLTPRHAAFGFAAARLDDGINLPASRLSALWIFLAAPRARRGA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
VWRALRDDARHHRSPNAGWPEAAMAGALGLRLAGPRLYGGVRVQDAWMGDGTPDADIGHL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCHHHH
DRALVLYRRACLIERIVIVALAVYITVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDASLSATLSVAALAAVIEWRVGYPAPVFAWIGHPVMWIGALIGRLDRLGNRPGWSSPG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
CRAAGFAALAIVVAVPVGLACGAVWLMDRVLPRGLAILLQAVAAGTLVAQRSLHDHVADV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTGLRGGGLAGGRAAVGRIVGRDTAGLDRAGIVRAAIESLAENFSDGVVAPLLWTALLGL
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
PGAVFYKAVNTADSMIGHLTPRHAAFGFAAARLDDGINLPASRLSALWIFLAAPRARRGA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
VWRALRDDARHHRSPNAGWPEAAMAGALGLRLAGPRLYGGVRVQDAWMGDGTPDADIGHL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCHHHH
DRALVLYRRACLIERIVIVALAVYITVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]