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The map label for this gene is thiC [H]

Identifier: 162146438

GI number: 162146438

Start: 621861

End: 623741

Strand: Direct

Name: thiC [H]

Synonym: GDI_0613

Alternate gene names: 162146438

Gene position: 621861-623741 (Clockwise)

Preceding gene: 162146437

Following gene: 162146440

Centisome position: 15.77

GC content: 66.4

Gene sequence:

>1881_bases
ATGAACATCCAGAGCCCCCCGCCTGCCTCTCCGCGTCAGCCCGCGCATGCGCCCGCCACGGGTGACGTGACCGTCGGCCC
CCGTGTGGGTGGCCACAAGGTCTATCAGCCCGGTACGCTCTACCCCGACCTGCGCGTGCCGTTCCGCCAGGTGGACCTGC
ACCCCACGGCCAACGAAGCGCCGGTGACGCTGTACGATCCCTCCGGCCCCTATACCGACCCGCAGGCCGATATCGACATC
GCGCGCGGGCTGGACAGTGTGCGTGACGCGTGGATCGTCCGCCGTGGGGACACCGAGACCGTGGCCCAGCCCCGCATTGT
CCGGCCCGAGGACAATGGCGGCGCCACCGGATCGATGCTGGCCCCCGCGTTCGACAACGGCCGGCGGGTGGTTCGCCGTG
GACGGGCCGGCCGGCCGGTGACGCAGCTGGAATACGCCCGCGCCGGCATCGTGACCGAGGAAATGGAATATGTCGCGATC
CGCGAAAACCTGCGCCGCGCGCAGGCCGACAAGCCGCTGCGCGACGGCGAGGATTTCGGTGCCAGCATCCCGGATTTCGT
GACGCCGGAATTCGTCCGCGACGAAATCGCCCGGGGGCGCGCCGTCCTGCCGTCCAACATCAACCACCCGGAACTGGAGC
CGATGATCATCGGCCGCAACTTCCTGGTGAAGATCAACGCCAACATCGGCAACTCCGCCGTGCTGGGACATGTGGCCGAC
GAAGTCGACAAGATGGTCTGGGCCACGCGCTGGGGCGCGGATACGGTGATGGACCTGTCCACCGGGCGCAATATCCACAA
TATCCGCGACTGGATCGTCCGCAACAGCCCCGTGCCCATCGGCACCGTTCCGATCTACCAGGCGCTGGAGAAGGTCGGCG
GCGTGGCCGAGGACCTGACATGGGAACTGTTCCGCGACACGCTGATCGAACAGGCCGAACAGGGGGTGGATTACTTCACC
ATCCATGCCGGCGTGCGCCTGCCCTTCATCCCGCTGACCGCTCAGCGCGTGACCGGCATCGTCTCGCGCGGCGGCTCGAT
CATGGCCAAATGGTGCCTGGCGCACCATCGCGAGAGCTTCCTGTACGAGAATTTCGCCGAAATCTGCGAAATCATGCGCG
CCTATGACGTGTCGTTCAGCCTGGGCGACGGGCTGCGCCCCGGTTCCATCGCCGATGCGAACGACGCGGCGCAGTTCGCC
GAACTGCGCACGCTGGGCGAACTGACCCGCATTGCCTGGGACCATGGCTGCCAGGTGATCATCGAAGGCCCGGGCCACGT
GCCGATGCACAAGATCAAGGCCAACATGGACGAGCAACTGGCCCATTGCGGCGAGGCGCCGTTCTACACGCTCGGTCCGT
TGACCACCGATATCGCGCCCGGATACGACCACATCACCAGCGCGATCGGCGCCGCGATGATCGGCTGGTTCGGAACCGCC
ATGCTCTGCTACGTCACGCCCAAGGAACATCTGGGCCTGCCGGACCGCAACGACGTCAAGACCGGGGTGATCACCTACAA
GCTCGCGGCGCATGCCGCGGACCTGGCCAAGGGCCATCCCGCCGCCCGCCTGCATGACGACGCGGTGTCGCGCGCCCGTT
TCGAATTCCGGTGGGAGGACCAATTCAATCTCGGCCTCGATCCGGAAACCGCGCGCTGCTTCCATGACGAAACCCTGCCG
AAGGAAGCCCACAAGACGGCGCATTTCTGTTCGATGTGCGGGCCGAAATTCTGCTCCATGAAGATCAGCCAGGATATCCG
CGACGCGGCCCGTGCCCAGAATGACGGCCTGTCCACGGCCGACATCAAGGCCGGCCTGGAGGAGATGAGCGAACGGTTCC
GTGCCGGTGGCGGGGCCATCGACATCGCGGTGGCCGACTGA

Upstream 100 bases:

>100_bases
ATGCCGGCGAAGGGATGGGAATTGAGCGCGATGCCGCGATGCGTCGCCAGGATGACATTTCGCGCCGGCGCGGCCCCTCC
AAAAGCAACGAGGACGCGCT

Downstream 100 bases:

>100_bases
CCAAAGGCGGGCCAGGGCTCAGCCCTTGGCCCGCCGCAGGGGCTGACCCCTTGGAAAAATCGATTGGGGGGGGCAGGGCA
TCGGGCCCTGCCTGCCTTCA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MNIQSPPPASPRQPAHAPATGDVTVGPRVGGHKVYQPGTLYPDLRVPFRQVDLHPTANEAPVTLYDPSGPYTDPQADIDI
ARGLDSVRDAWIVRRGDTETVAQPRIVRPEDNGGATGSMLAPAFDNGRRVVRRGRAGRPVTQLEYARAGIVTEEMEYVAI
RENLRRAQADKPLRDGEDFGASIPDFVTPEFVRDEIARGRAVLPSNINHPELEPMIIGRNFLVKINANIGNSAVLGHVAD
EVDKMVWATRWGADTVMDLSTGRNIHNIRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLTWELFRDTLIEQAEQGVDYFT
IHAGVRLPFIPLTAQRVTGIVSRGGSIMAKWCLAHHRESFLYENFAEICEIMRAYDVSFSLGDGLRPGSIADANDAAQFA
ELRTLGELTRIAWDHGCQVIIEGPGHVPMHKIKANMDEQLAHCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTA
MLCYVTPKEHLGLPDRNDVKTGVITYKLAAHAADLAKGHPAARLHDDAVSRARFEFRWEDQFNLGLDPETARCFHDETLP
KEAHKTAHFCSMCGPKFCSMKISQDIRDAARAQNDGLSTADIKAGLEEMSERFRAGGGAIDIAVAD

Sequences:

>Translated_626_residues
MNIQSPPPASPRQPAHAPATGDVTVGPRVGGHKVYQPGTLYPDLRVPFRQVDLHPTANEAPVTLYDPSGPYTDPQADIDI
ARGLDSVRDAWIVRRGDTETVAQPRIVRPEDNGGATGSMLAPAFDNGRRVVRRGRAGRPVTQLEYARAGIVTEEMEYVAI
RENLRRAQADKPLRDGEDFGASIPDFVTPEFVRDEIARGRAVLPSNINHPELEPMIIGRNFLVKINANIGNSAVLGHVAD
EVDKMVWATRWGADTVMDLSTGRNIHNIRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLTWELFRDTLIEQAEQGVDYFT
IHAGVRLPFIPLTAQRVTGIVSRGGSIMAKWCLAHHRESFLYENFAEICEIMRAYDVSFSLGDGLRPGSIADANDAAQFA
ELRTLGELTRIAWDHGCQVIIEGPGHVPMHKIKANMDEQLAHCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTA
MLCYVTPKEHLGLPDRNDVKTGVITYKLAAHAADLAKGHPAARLHDDAVSRARFEFRWEDQFNLGLDPETARCFHDETLP
KEAHKTAHFCSMCGPKFCSMKISQDIRDAARAQNDGLSTADIKAGLEEMSERFRAGGGAIDIAVAD
>Mature_626_residues
MNIQSPPPASPRQPAHAPATGDVTVGPRVGGHKVYQPGTLYPDLRVPFRQVDLHPTANEAPVTLYDPSGPYTDPQADIDI
ARGLDSVRDAWIVRRGDTETVAQPRIVRPEDNGGATGSMLAPAFDNGRRVVRRGRAGRPVTQLEYARAGIVTEEMEYVAI
RENLRRAQADKPLRDGEDFGASIPDFVTPEFVRDEIARGRAVLPSNINHPELEPMIIGRNFLVKINANIGNSAVLGHVAD
EVDKMVWATRWGADTVMDLSTGRNIHNIRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLTWELFRDTLIEQAEQGVDYFT
IHAGVRLPFIPLTAQRVTGIVSRGGSIMAKWCLAHHRESFLYENFAEICEIMRAYDVSFSLGDGLRPGSIADANDAAQFA
ELRTLGELTRIAWDHGCQVIIEGPGHVPMHKIKANMDEQLAHCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTA
MLCYVTPKEHLGLPDRNDVKTGVITYKLAAHAADLAKGHPAARLHDDAVSRARFEFRWEDQFNLGLDPETARCFHDETLP
KEAHKTAHFCSMCGPKFCSMKISQDIRDAARAQNDGLSTADIKAGLEEMSERFRAGGGAIDIAVAD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=605, Percent_Identity=65.7851239669422, Blast_Score=810, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 68634; Mature: 68634

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQSPPPASPRQPAHAPATGDVTVGPRVGGHKVYQPGTLYPDLRVPFRQVDLHPTANEA
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCC
PVTLYDPSGPYTDPQADIDIARGLDSVRDAWIVRRGDTETVAQPRIVRPEDNGGATGSML
CEEEECCCCCCCCCCCCHHHHHCHHHHHHHHEEECCCCHHHCCCEEECCCCCCCCCCCEE
APAFDNGRRVVRRGRAGRPVTQLEYARAGIVTEEMEYVAIRENLRRAQADKPLRDGEDFG
CCCHHCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
ASIPDFVTPEFVRDEIARGRAVLPSNINHPELEPMIIGRNFLVKINANIGNSAVLGHVAD
CCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHH
EVDKMVWATRWGADTVMDLSTGRNIHNIRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLT
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
WELFRDTLIEQAEQGVDYFTIHAGVRLPFIPLTAQRVTGIVSRGGSIMAKWCLAHHRESF
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LYENFAEICEIMRAYDVSFSLGDGLRPGSIADANDAAQFAELRTLGELTRIAWDHGCQVI
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEGPGHVPMHKIKANMDEQLAHCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTA
EECCCCCCHHHHHCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHE
MLCYVTPKEHLGLPDRNDVKTGVITYKLAAHAADLAKGHPAARLHDDAVSRARFEFRWED
EEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECC
QFNLGLDPETARCFHDETLPKEAHKTAHFCSMCGPKFCSMKISQDIRDAARAQNDGLSTA
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHH
DIKAGLEEMSERFRAGGGAIDIAVAD
HHHHHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MNIQSPPPASPRQPAHAPATGDVTVGPRVGGHKVYQPGTLYPDLRVPFRQVDLHPTANEA
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEECCCCCCCCCCCCCCEEEECCCCCCCC
PVTLYDPSGPYTDPQADIDIARGLDSVRDAWIVRRGDTETVAQPRIVRPEDNGGATGSML
CEEEECCCCCCCCCCCCHHHHHCHHHHHHHHEEECCCCHHHCCCEEECCCCCCCCCCCEE
APAFDNGRRVVRRGRAGRPVTQLEYARAGIVTEEMEYVAIRENLRRAQADKPLRDGEDFG
CCCHHCCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
ASIPDFVTPEFVRDEIARGRAVLPSNINHPELEPMIIGRNFLVKINANIGNSAVLGHVAD
CCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHH
EVDKMVWATRWGADTVMDLSTGRNIHNIRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLT
HHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHH
WELFRDTLIEQAEQGVDYFTIHAGVRLPFIPLTAQRVTGIVSRGGSIMAKWCLAHHRESF
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHH
LYENFAEICEIMRAYDVSFSLGDGLRPGSIADANDAAQFAELRTLGELTRIAWDHGCQVI
HHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEGPGHVPMHKIKANMDEQLAHCGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTA
EECCCCCCHHHHHCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHE
MLCYVTPKEHLGLPDRNDVKTGVITYKLAAHAADLAKGHPAARLHDDAVSRARFEFRWED
EEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEECC
QFNLGLDPETARCFHDETLPKEAHKTAHFCSMCGPKFCSMKISQDIRDAARAQNDGLSTA
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHH
DIKAGLEEMSERFRAGGGAIDIAVAD
HHHHHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA