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The map label for this gene is glnA [H]

Identifier: 162146350

GI number: 162146350

Start: 519694

End: 521154

Strand: Reverse

Name: glnA [H]

Synonym: GDI_0525

Alternate gene names: 162146350

Gene position: 521154-519694 (Counterclockwise)

Preceding gene: 162146351

Following gene: 162146348

Centisome position: 13.21

GC content: 63.38

Gene sequence:

>1461_bases
ATGGCGAAAAAAGCCCCGAGTTCAGCTCCGACCACGACTCCGTCCCCATCGGCGGAGGCTGTTGCCAAGGTATTCAGCCT
GATCCAGGAACATTCCGTGGAACTGGTGGACCTGCGCTTCACGGACCCGCGCGGCAAGTGGCACCACACCACGCAGCATA
TCTCGACGATCGAGCAGGACACGTTCCGCGACGGCTTCATGTTCGACGGATCGTCCATCGTCGGCTGGAAGGCCATCAAC
GAGAGCGACATGGTGCTGCTGCCCGACCCGACGACGGCGGTCATGGACCCGTTCTCGGCGAAGCCGCAGCTGATCCTGAT
CTGCGACATCATCGAGCCCTCGACCGGGCAGTTCTACAACCGCGACCCCCGCGCCACCGCCAAGCTGGCCGAGGCCTACC
TGAAGTCCACCGGCCTGGGCGACACGGCGTTTTTCGGTCCGGAAGCCGAATTCTTCGTCTTCGACAGCGTCAAGTTCGGC
ACGGGCCCGAATTTCGGCATCTACCAGCTTGACAGCATCGAGGGGCCCGGCGCCTCGCTGAAGGACTATCCCGAGGGCAA
CATGGGCCATCGCCCGACGGTGAAGGGCGGCTATTTCCCCGTCCCGCCGGTCGACAGCGAAAACGACCTGCGCGCCGAGA
TGCTGTCCACGATGGGCGAGATGGGCCTGCCGATCGAAAAGCATCACCACGAGGTCGCGCAGTCGCAGCACGAGTTGGGC
ACCAAGTTCGCGACGCTGGTGAAGTCCGCCGACTTCATGCAGATCTACAAATACTGCGTGCACAACGTCGCCCATTCCTA
CGGCAAGTCCGCGACGTTCATGCCGAAGCCGATCTATGGCGACAACGGATCGGGCATGCACGTCCACCAGTCGATCTGGA
AGAGCGGCAAGCCGGTCTTCGCCGGCAACGGCTATGCCGACCTGTCGGACCAGGCGCTGTATTACATCGGCGGCATCATC
AAGCACGCCAAGGCGCTGAACGCCTTCACCAACCCGTCGACCAATTCCTACAAGCGCCTGATCCCGGGCTTCGAGGCGCC
GGTGCTGCTGGCCTATTCGGCGCGCAACCGCTCGGCGTCCTGCCGCATCCCGTATGCGACCAGCCCGAAGGCCAAGCGCG
TCGAGGTCCGCTTCCCCGACCCGACCGCCAACCCCTACCTGGCGTTCGCGGCCATGCTGATGGCCGGCCTGGACGGCATC
AAGAACAAGATCCATCCGGGCGACGCCATGGACAAGGATCTGTACGACCTGCCGCCCGACGAGCTGAAGCAGATCCCGAC
CGTCTGCGGATCGCTGCGCGAGGCGCTGGAGGCCCTGACGGCCGATCATGAGTTCCTGCTGGCCGGCGGCGTGTTCACCA
AGGACCAGATCGAGAGCTATATCGACATCAAGTGGCAGGAAGTGTTCAAGTTCGAACACACCCCGCACCCGGCCGAGTTC
GAGATGTACTACTCCGTCTGA

Upstream 100 bases:

>100_bases
GTGCCGGGGTCCGTATGTCCTATCGACAACGGCGCCCCAGTCTCGTAACCGTTTCACCGCCGTGTCGTTCGGCATCCCAA
GTTAAGGCAGGTAATCAGCA

Downstream 100 bases:

>100_bases
TCCCGGCGGGATTGACGAAAAAAGGGGGCGCCGGACCGATTGGGTCCGGCGCCCCTTTTTCGTTCGGCACGCCCTCAGAC
GCGGGCGTGGAGACGACCCT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAKKAPSSAPTTTPSPSAEAVAKVFSLIQEHSVELVDLRFTDPRGKWHHTTQHISTIEQDTFRDGFMFDGSSIVGWKAIN
ESDMVLLPDPTTAVMDPFSAKPQLILICDIIEPSTGQFYNRDPRATAKLAEAYLKSTGLGDTAFFGPEAEFFVFDSVKFG
TGPNFGIYQLDSIEGPGASLKDYPEGNMGHRPTVKGGYFPVPPVDSENDLRAEMLSTMGEMGLPIEKHHHEVAQSQHELG
TKFATLVKSADFMQIYKYCVHNVAHSYGKSATFMPKPIYGDNGSGMHVHQSIWKSGKPVFAGNGYADLSDQALYYIGGII
KHAKALNAFTNPSTNSYKRLIPGFEAPVLLAYSARNRSASCRIPYATSPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGI
KNKIHPGDAMDKDLYDLPPDELKQIPTVCGSLREALEALTADHEFLLAGGVFTKDQIESYIDIKWQEVFKFEHTPHPAEF
EMYYSV

Sequences:

>Translated_486_residues
MAKKAPSSAPTTTPSPSAEAVAKVFSLIQEHSVELVDLRFTDPRGKWHHTTQHISTIEQDTFRDGFMFDGSSIVGWKAIN
ESDMVLLPDPTTAVMDPFSAKPQLILICDIIEPSTGQFYNRDPRATAKLAEAYLKSTGLGDTAFFGPEAEFFVFDSVKFG
TGPNFGIYQLDSIEGPGASLKDYPEGNMGHRPTVKGGYFPVPPVDSENDLRAEMLSTMGEMGLPIEKHHHEVAQSQHELG
TKFATLVKSADFMQIYKYCVHNVAHSYGKSATFMPKPIYGDNGSGMHVHQSIWKSGKPVFAGNGYADLSDQALYYIGGII
KHAKALNAFTNPSTNSYKRLIPGFEAPVLLAYSARNRSASCRIPYATSPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGI
KNKIHPGDAMDKDLYDLPPDELKQIPTVCGSLREALEALTADHEFLLAGGVFTKDQIESYIDIKWQEVFKFEHTPHPAEF
EMYYSV
>Mature_485_residues
AKKAPSSAPTTTPSPSAEAVAKVFSLIQEHSVELVDLRFTDPRGKWHHTTQHISTIEQDTFRDGFMFDGSSIVGWKAINE
SDMVLLPDPTTAVMDPFSAKPQLILICDIIEPSTGQFYNRDPRATAKLAEAYLKSTGLGDTAFFGPEAEFFVFDSVKFGT
GPNFGIYQLDSIEGPGASLKDYPEGNMGHRPTVKGGYFPVPPVDSENDLRAEMLSTMGEMGLPIEKHHHEVAQSQHELGT
KFATLVKSADFMQIYKYCVHNVAHSYGKSATFMPKPIYGDNGSGMHVHQSIWKSGKPVFAGNGYADLSDQALYYIGGIIK
HAKALNAFTNPSTNSYKRLIPGFEAPVLLAYSARNRSASCRIPYATSPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIK
NKIHPGDAMDKDLYDLPPDELKQIPTVCGSLREALEALTADHEFLLAGGVFTKDQIESYIDIKWQEVFKFEHTPHPAEFE
MYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=63.3620689655172, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI87081870, Length=322, Percent_Identity=24.8447204968944, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53608; Mature: 53477

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKAPSSAPTTTPSPSAEAVAKVFSLIQEHSVELVDLRFTDPRGKWHHTTQHISTIEQD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
TFRDGFMFDGSSIVGWKAINESDMVLLPDPTTAVMDPFSAKPQLILICDIIEPSTGQFYN
HCCCCEEECCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCC
RDPRATAKLAEAYLKSTGLGDTAFFGPEAEFFVFDSVKFGTGPNFGIYQLDSIEGPGASL
CCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECEEECCCCCCCEEEECCCCCCCCCC
KDYPEGNMGHRPTVKGGYFPVPPVDSENDLRAEMLSTMGEMGLPIEKHHHEVAQSQHELG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TKFATLVKSADFMQIYKYCVHNVAHSYGKSATFMPKPIYGDNGSGMHVHQSIWKSGKPVF
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
AGNGYADLSDQALYYIGGIIKHAKALNAFTNPSTNSYKRLIPGFEAPVLLAYSARNRSAS
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCC
CRIPYATSPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGDAMDKDLYDLPPD
EECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ELKQIPTVCGSLREALEALTADHEFLLAGGVFTKDQIESYIDIKWQEVFKFEHTPHPAEF
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCE
EMYYSV
EEEEEC
>Mature Secondary Structure 
AKKAPSSAPTTTPSPSAEAVAKVFSLIQEHSVELVDLRFTDPRGKWHHTTQHISTIEQD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHH
TFRDGFMFDGSSIVGWKAINESDMVLLPDPTTAVMDPFSAKPQLILICDIIEPSTGQFYN
HCCCCEEECCCCEEEEEEECCCCEEEECCCCHHHCCCCCCCCCEEEEEEECCCCCCCCCC
RDPRATAKLAEAYLKSTGLGDTAFFGPEAEFFVFDSVKFGTGPNFGIYQLDSIEGPGASL
CCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECEEECCCCCCCEEEECCCCCCCCCC
KDYPEGNMGHRPTVKGGYFPVPPVDSENDLRAEMLSTMGEMGLPIEKHHHEVAQSQHELG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TKFATLVKSADFMQIYKYCVHNVAHSYGKSATFMPKPIYGDNGSGMHVHQSIWKSGKPVF
HHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEE
AGNGYADLSDQALYYIGGIIKHAKALNAFTNPSTNSYKRLIPGFEAPVLLAYSARNRSAS
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCC
CRIPYATSPKAKRVEVRFPDPTANPYLAFAAMLMAGLDGIKNKIHPGDAMDKDLYDLPPD
EECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ELKQIPTVCGSLREALEALTADHEFLLAGGVFTKDQIESYIDIKWQEVFKFEHTPHPAEF
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCE
EMYYSV
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2878685 [H]