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The map label for this gene is nifV [H]

Identifier: 162146274

GI number: 162146274

Start: 445368

End: 446507

Strand: Direct

Name: nifV [H]

Synonym: GDI_0449

Alternate gene names: 162146274

Gene position: 445368-446507 (Clockwise)

Preceding gene: 162146273

Following gene: 162146275

Centisome position: 11.29

GC content: 70.09

Gene sequence:

>1140_bases
ATGTCGGAAAACGCGCGTCTGCTGATCAGCGACACCACCCTGCGCGATGGAGAGCAGGCGCCGGGGGTGGCCTTTACCGC
GTGGGAAAAGCTGGCCATCGCCGGCGCCCTCGACGCGGCCGGCGTGGATGAGATCGAGGCCGGCGTTCCGGCCATGGGGG
ATGCCGAGATCGCCATGATCGCGGCGATCGGCGACGAGGTGGAACGCGCCCGCGTCATCCCCTGGTGTCGCATGCGCGAC
GAAGACGTGCACGCGGCGCGGCGGACGGGGCTGGGCAGCGTGCACCTGTCCGTTTCGACCTCGGTCCGGCAGATCCAGGC
GAAATACCGCATGTCGCCGCGTCGCACGCTGGACATGGCGCGCGACGTCGTCTCCCGCGCCAGGGATTACGGCCTGGCGG
TGTCGGTGGGGGGCGAGGACGCCAGCCGGGCCGATCCGGCCTATCTGGTCGATCTGCTGGGCGTGATCGCCGAGGCAGGG
GCGTTCCGCTTCCGCTTCGCCGATACGCTGGGCGTGATGGACCCGTTCGGCGTGCACGAGGTCATGCGGTATCTCTGCCA
GTCCAGCCCGCTTCAGCTTGAATTTCACGGCCATGACGATCTGGGCCTGGCAACGGCCAATACGCTGGCCGCCGTGCGGG
CCGGCGCGGCGTGCGCCAGTGTCACCGTCCTGGGACTGGGCGAACGCGCGGGCAACGCCCCGCTGGAGGAAGTGGTGGCG
GGGGTATATCGCCTGCTGGGCCGTCCGGCGGGCGTGAAGCTGGACAGCCTGCCCGGGCTTGCCACCCTGGTCTCGCGCGC
CGCCGTGCGTGATATCCCGCCCGACAAGGCGATCGTCGGCGACGCGGTCTTTCGTCATGAATCGGGCATCCATGTCTCGG
GCCTGCTGCGTGACGCCGCGACGTATGAGGCACTGGACCCCGTGCAGTTCGGCCGCCAGCGCGAAATCGTGCTGGGCAAG
CATTCCGGCCGGGCCGCGGTACGGCACGCGCTGGCGGCGCTGGGCCTGGATGCCGACGAAACCGTCATCGCGGCCACGCT
TGCCGCCGTGCGCGCGCGTGCCTCCGCCGCCAAGCGCACGGTGGCGCTGGCCGAACTGGCCGAAATGCATGCGGGCCTGA
TGGCAGGCGTGTCGAAATAA

Upstream 100 bases:

>100_bases
TCTGGGATGGCGCGGCCGCCGCGAAATCCGGCTTTGCACCGACTTATGCCTAGGGCGATGCGCCGGGCCTGATGAAGCCC
GGCGGTATGGAGGCTGTGCG

Downstream 100 bases:

>100_bases
GACATCGGGGAACCACGAAATGATGGGCATTCTGGACGAACTGCGCGGCCTGTCGGCCGCCGAGGACTTTTTCCGCACGC
TGGGCGTCAAGTACGACGCC

Product: homocitrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSENARLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMIAAIGDEVERARVIPWCRMRD
EDVHAARRTGLGSVHLSVSTSVRQIQAKYRMSPRRTLDMARDVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAG
AFRFRFADTLGVMDPFGVHEVMRYLCQSSPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEEVVA
GVYRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIHVSGLLRDAATYEALDPVQFGRQREIVLGK
HSGRAAVRHALAALGLDADETVIAATLAAVRARASAAKRTVALAELAEMHAGLMAGVSK

Sequences:

>Translated_379_residues
MSENARLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMIAAIGDEVERARVIPWCRMRD
EDVHAARRTGLGSVHLSVSTSVRQIQAKYRMSPRRTLDMARDVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAG
AFRFRFADTLGVMDPFGVHEVMRYLCQSSPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEEVVA
GVYRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIHVSGLLRDAATYEALDPVQFGRQREIVLGK
HSGRAAVRHALAALGLDADETVIAATLAAVRARASAAKRTVALAELAEMHAGLMAGVSK
>Mature_378_residues
SENARLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMIAAIGDEVERARVIPWCRMRDE
DVHAARRTGLGSVHLSVSTSVRQIQAKYRMSPRRTLDMARDVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAGA
FRFRFADTLGVMDPFGVHEVMRYLCQSSPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEEVVAG
VYRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIHVSGLLRDAATYEALDPVQFGRQREIVLGKH
SGRAAVRHALAALGLDADETVIAATLAAVRARASAAKRTVALAELAEMHAGLMAGVSK

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=347, Percent_Identity=34.8703170028818, Blast_Score=178, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320071, Length=356, Percent_Identity=30.0561797752809, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=356, Percent_Identity=30.0561797752809, Blast_Score=153, Evalue=5e-38,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 39836; Mature: 39705

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENARLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMI
CCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEE
AAIGDEVERARVIPWCRMRDEDVHAARRTGLGSVHLSVSTSVRQIQAKYRMSPRRTLDMA
EHHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
RDVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAGAFRFRFADTLGVMDPFGVHE
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHH
VMRYLCQSSPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEEVVA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCHHHHHH
GVYRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIHVSGLLRDAA
HHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
TYEALDPVQFGRQREIVLGKHSGRAAVRHALAALGLDADETVIAATLAAVRARASAAKRT
HHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAELAEMHAGLMAGVSK
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SENARLLISDTTLRDGEQAPGVAFTAWEKLAIAGALDAAGVDEIEAGVPAMGDAEIAMI
CCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEE
AAIGDEVERARVIPWCRMRDEDVHAARRTGLGSVHLSVSTSVRQIQAKYRMSPRRTLDMA
EHHHHHHHHHHCCCHHHCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHH
RDVVSRARDYGLAVSVGGEDASRADPAYLVDLLGVIAEAGAFRFRFADTLGVMDPFGVHE
HHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCHHH
VMRYLCQSSPLQLEFHGHDDLGLATANTLAAVRAGAACASVTVLGLGERAGNAPLEEVVA
HHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEEEECCCCCCCCCHHHHHH
GVYRLLGRPAGVKLDSLPGLATLVSRAAVRDIPPDKAIVGDAVFRHESGIHVSGLLRDAA
HHHHHHCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
TYEALDPVQFGRQREIVLGKHSGRAAVRHALAALGLDADETVIAATLAAVRARASAAKRT
HHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VALAELAEMHAGLMAGVSK
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1317839 [H]