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The map label for this gene is nifN [H]

Identifier: 162146265

GI number: 162146265

Start: 439114

End: 440511

Strand: Direct

Name: nifN [H]

Synonym: GDI_0440

Alternate gene names: 162146265

Gene position: 439114-440511 (Clockwise)

Preceding gene: 162146264

Following gene: 162146266

Centisome position: 11.13

GC content: 67.38

Gene sequence:

>1398_bases
GTGGCCACCATCGTGAAGCCGCGCAAGGCGGCGTCCGTCAATCCGCTGAAATCCTCGACGCCGCTGGGCGCGGCGCTGGC
CTATCTGGGTATCGACGGCGCGGTGCCGCTGTTCCATGGCTCGCAGGGCTGCACGTCCTTCGCGCTGGTCCTGACCGTGC
GCCATTACAAGGAAGCGATCCCGCTGCAGACCACGGCGATGGACGAGGTCGCGACCATCCTGGGGGCCGCGGGCAATTTG
GAAGAGGCACTGCTGAATTTGCAGCGGCGGATGAAGCCGCGCTTCATCGGCATCGCCTCGACCGCACTGGTCGAGACCAG
GGGAGAGGATTATGCCGGCGACCTGAAGCTGATCCTCCAGCGGCAGCCCGAACTGGCGGATACACGGATCGTCTTCGCCT
CGACCCCGGATTATGCCGGCGCGCTGGAGGACGGCTGGGCGGCCGCCGTCAGCGCGATCATCGAATCGGTCGTGGCCCCG
TGGTCGCCGACGGTGACGTCGTTCCAGCAGGTCAACGTCCTGCCGGGCGTGCACCAGACCCCGGCCGACATCGAGGCGCT
GCGGGACCTGATCGAAAGCTTCGGCCTGTATCCCGTGATCCTGCCGGACCTGTCCGGATCGCTGGACGGTCATGTGGCCG
AGAACTGGTGCCCGACCACGCAGGGCGGCGCGCGGATGGAAGAAGTGGCGCAGATGGCGCGCGCGGTGCACACCATCGCC
ATCGGCGAGCATATGCGCGCGCCGGCCGACCTGCTGGGCAGCGTAACCGGCGTGCCGGTCACGCTGTTTCCCACCCTGAC
GGGACTTGCGGCCAACGACCGGCTGATGGCGCTGCTGTCGCGCCTGTCGGGCCGGGCGGTGCCGGGGCGCTATCGCCGCC
AGCGCAGCCAGTTGCTGGACGCGATGCTGGACGGTCATTTCCATTTCGGCGGCAAGCGCATCGCGATCGCCGCCGATCCC
GATCTTCTGTATGGCCTGTCGGCCTTCTTTGCCGGAATGGGCGCACGGATCGTCGCCGCGGTCGCCTCGACGTCCAATGC
GCCGAACCTGGACTCCATTCCCGCGGACAGCGTCATCGTGGGCGACCTGACGGATCTTGAAGACGCGGTCCACGCGGCGG
GGGGCGCCGATCTGCTGGTCACCCACAGTCACGGCCGTCAGTCCGCCGACCGCCTGGGCATTCCGCTGATGCGCGTGGGC
TTTCCCATTTTCGATCGTCTGGGCACCGCGCACGCGCAGACCATCGGATATCGCGGCACGCGCGACCTGATCTTCCGCGT
CGCCAACCTGTTCCTGGGCCAGATGCATGAGCACACGCCGGACGATTTCGGCCACGTGCCGTCCGCCCATACGATCGAGG
AGATAGTGCATGACAGCGCGTCGCTTGCAGCTCATTGA

Upstream 100 bases:

>100_bases
GGGCGGATGTCCGCCGCCCGGCCCCGTGGGAGGAGGACGATGCCGATGCGTTCCTGGATGACGCGCCGTTCCTGACCCCC
TTGTCCTGAGGAGTTCGTCC

Downstream 100 bases:

>100_bases
ACCGGAAGCCGGCGGGGACGGTGCCGCCGCCGGTGTCGTGCCGCGCCTGCGGATCGCGATCGCGACCCAGGACATGAAGG
CGCTGAACGCGCATTTCGGG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MATIVKPRKAASVNPLKSSTPLGAALAYLGIDGAVPLFHGSQGCTSFALVLTVRHYKEAIPLQTTAMDEVATILGAAGNL
EEALLNLQRRMKPRFIGIASTALVETRGEDYAGDLKLILQRQPELADTRIVFASTPDYAGALEDGWAAAVSAIIESVVAP
WSPTVTSFQQVNVLPGVHQTPADIEALRDLIESFGLYPVILPDLSGSLDGHVAENWCPTTQGGARMEEVAQMARAVHTIA
IGEHMRAPADLLGSVTGVPVTLFPTLTGLAANDRLMALLSRLSGRAVPGRYRRQRSQLLDAMLDGHFHFGGKRIAIAADP
DLLYGLSAFFAGMGARIVAAVASTSNAPNLDSIPADSVIVGDLTDLEDAVHAAGGADLLVTHSHGRQSADRLGIPLMRVG
FPIFDRLGTAHAQTIGYRGTRDLIFRVANLFLGQMHEHTPDDFGHVPSAHTIEEIVHDSASLAAH

Sequences:

>Translated_465_residues
MATIVKPRKAASVNPLKSSTPLGAALAYLGIDGAVPLFHGSQGCTSFALVLTVRHYKEAIPLQTTAMDEVATILGAAGNL
EEALLNLQRRMKPRFIGIASTALVETRGEDYAGDLKLILQRQPELADTRIVFASTPDYAGALEDGWAAAVSAIIESVVAP
WSPTVTSFQQVNVLPGVHQTPADIEALRDLIESFGLYPVILPDLSGSLDGHVAENWCPTTQGGARMEEVAQMARAVHTIA
IGEHMRAPADLLGSVTGVPVTLFPTLTGLAANDRLMALLSRLSGRAVPGRYRRQRSQLLDAMLDGHFHFGGKRIAIAADP
DLLYGLSAFFAGMGARIVAAVASTSNAPNLDSIPADSVIVGDLTDLEDAVHAAGGADLLVTHSHGRQSADRLGIPLMRVG
FPIFDRLGTAHAQTIGYRGTRDLIFRVANLFLGQMHEHTPDDFGHVPSAHTIEEIVHDSASLAAH
>Mature_464_residues
ATIVKPRKAASVNPLKSSTPLGAALAYLGIDGAVPLFHGSQGCTSFALVLTVRHYKEAIPLQTTAMDEVATILGAAGNLE
EALLNLQRRMKPRFIGIASTALVETRGEDYAGDLKLILQRQPELADTRIVFASTPDYAGALEDGWAAAVSAIIESVVAPW
SPTVTSFQQVNVLPGVHQTPADIEALRDLIESFGLYPVILPDLSGSLDGHVAENWCPTTQGGARMEEVAQMARAVHTIAI
GEHMRAPADLLGSVTGVPVTLFPTLTGLAANDRLMALLSRLSGRAVPGRYRRQRSQLLDAMLDGHFHFGGKRIAIAADPD
LLYGLSAFFAGMGARIVAAVASTSNAPNLDSIPADSVIVGDLTDLEDAVHAAGGADLLVTHSHGRQSADRLGIPLMRVGF
PIFDRLGTAHAQTIGYRGTRDLIFRVANLFLGQMHEHTPDDFGHVPSAHTIEEIVHDSASLAAH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 49360; Mature: 49229

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIVKPRKAASVNPLKSSTPLGAALAYLGIDGAVPLFHGSQGCTSFALVLTVRHYKEAI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHC
PLQTTAMDEVATILGAAGNLEEALLNLQRRMKPRFIGIASTALVETRGEDYAGDLKLILQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHHHHHHH
RQPELADTRIVFASTPDYAGALEDGWAAAVSAIIESVVAPWSPTVTSFQQVNVLPGVHQT
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
PADIEALRDLIESFGLYPVILPDLSGSLDGHVAENWCPTTQGGARMEEVAQMARAVHTIA
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IGEHMRAPADLLGSVTGVPVTLFPTLTGLAANDRLMALLSRLSGRAVPGRYRRQRSQLLD
HHHHHCCCHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDGHFHFGGKRIAIAADPDLLYGLSAFFAGMGARIVAAVASTSNAPNLDSIPADSVIV
HHHCCCEEECCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
GDLTDLEDAVHAAGGADLLVTHSHGRQSADRLGIPLMRVGFPIFDRLGTAHAQTIGYRGT
CCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHCCHHHHHHCHHHHHHHCCCCH
RDLIFRVANLFLGQMHEHTPDDFGHVPSAHTIEEIVHDSASLAAH
HHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
ATIVKPRKAASVNPLKSSTPLGAALAYLGIDGAVPLFHGSQGCTSFALVLTVRHYKEAI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHC
PLQTTAMDEVATILGAAGNLEEALLNLQRRMKPRFIGIASTALVETRGEDYAGDLKLILQ
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCHHHHHHH
RQPELADTRIVFASTPDYAGALEDGWAAAVSAIIESVVAPWSPTVTSFQQVNVLPGVHQT
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCC
PADIEALRDLIESFGLYPVILPDLSGSLDGHVAENWCPTTQGGARMEEVAQMARAVHTIA
CHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IGEHMRAPADLLGSVTGVPVTLFPTLTGLAANDRLMALLSRLSGRAVPGRYRRQRSQLLD
HHHHHCCCHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDGHFHFGGKRIAIAADPDLLYGLSAFFAGMGARIVAAVASTSNAPNLDSIPADSVIV
HHHCCCEEECCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEE
GDLTDLEDAVHAAGGADLLVTHSHGRQSADRLGIPLMRVGFPIFDRLGTAHAQTIGYRGT
CCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCHHHHCCHHHHHHCHHHHHHHCCCCH
RDLIFRVANLFLGQMHEHTPDDFGHVPSAHTIEEIVHDSASLAAH
HHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]