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The map label for this gene is dmlA [H]

Identifier: 162146245

GI number: 162146245

Start: 419462

End: 420538

Strand: Reverse

Name: dmlA [H]

Synonym: GDI_0420

Alternate gene names: 162146245

Gene position: 420538-419462 (Counterclockwise)

Preceding gene: 162146246

Following gene: 162146244

Centisome position: 10.66

GC content: 67.04

Gene sequence:

>1077_bases
ATGACACAGCATACCATCGCCGTCCTGCCGGGCGACGGAATCGGCCGGGAGGTGATGCCCGAGGCCCTGAAGGTTCTGGA
CCGGGTGGGACAGATCTTCGATATCGGGTTCCGTTTCTCGCATCTCGACTGGAGTTGCGAGACCTGGCTGGCCACCGGTA
GCATGATGCCGCCGGACGGCATGGACCGCCTGTCACAGCACGATGCGATCCTGCTGGGCGCCGTGGGCTATCCCACCGTG
CCCGATCATATCTCGCTGTGGGGGATGCTGATCCCGATCCGGCGGGAATTCGACCAGTACGTGAACCTGCGCCCCGTAAG
GCTGCTGCCCGGCATCCCGTGCCCGCTGGCCGGGCGTGCGCCGGGCGACATCGATTTCTGGATCGTGCGCGAAAACACCG
AAGGCGAATACTCGCAGATCGGCGGCCGCTTCGCCGAAGGCAGCGAGGCGGAAGGCGTGGCGCAGACCGCGATCTTCACC
CGCCGGGGGACGGACCGCATCATGCGCTATGCCTTCGACCTGGCAACCCGCCTGGGCCGCCCGCATGTCTCCTCCGCCAC
CAAGTCGAACGGGCTGTTCCATTCCATGCCCTACTGGGATGAGCGGTTCGAGAAGATGGCCGCCGAATATCCCGATATCC
GGACCGACAGCCACCATATCGACATCCTGGCCGCGCGGTTCGTCATGCATCCCGACCGCTTCGACGTCGTCGTCGGCAGC
AACCTGTTCGGCGACATCCTGTCCGATCTGGGCCCGGGCGTGACCGGGACGATCGCGGTGGCCCCCTCGGCGAACATCAA
TCCGGAAAAGCGCTTTCCGTCGATGTTCGAACCGGTCCACGGCTCGGCGCCGGATATCGCCGGGCGTGCCATCGCAAACC
CGATCGGGCAGGTCTGGGCGGCGTCGATGATGCTGGACCATCTGGGCGAGGGCGAGGCCGCGGCGCTGGTGCTGCGCGCC
ATCGAACACATGACGGCCGACCCGTCGGCCCTGGTGCGCACCGCCGATATCGGCGGCCACGCCAGCACGTCGGCCTGCGG
CGACGCGATCGTGGCGGCGCTGGACCGGATCGCCTGA

Upstream 100 bases:

>100_bases
CCATTTCGGTTGCAACTACGCAACATCATGGTCCCGGCGCCCCGCCCCCTTCCCCTACACTGAACGGCGCATCCGTGGAC
CAATCGGAGGACACACGATC

Downstream 100 bases:

>100_bases
CCGTCCGCCTGCGGGGCAGGATTCGACTCCTCCCGAATCCTGCCCCGCAACCGCAGCTTCTTCTGTCTCTCGATGCAAAA
TCGATACGACATGCCCGTCG

Product: tartrate dehydrogenase/decarboxylase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTQHTIAVLPGDGIGREVMPEALKVLDRVGQIFDIGFRFSHLDWSCETWLATGSMMPPDGMDRLSQHDAILLGAVGYPTV
PDHISLWGMLIPIRREFDQYVNLRPVRLLPGIPCPLAGRAPGDIDFWIVRENTEGEYSQIGGRFAEGSEAEGVAQTAIFT
RRGTDRIMRYAFDLATRLGRPHVSSATKSNGLFHSMPYWDERFEKMAAEYPDIRTDSHHIDILAARFVMHPDRFDVVVGS
NLFGDILSDLGPGVTGTIAVAPSANINPEKRFPSMFEPVHGSAPDIAGRAIANPIGQVWAASMMLDHLGEGEAAALVLRA
IEHMTADPSALVRTADIGGHASTSACGDAIVAALDRIA

Sequences:

>Translated_358_residues
MTQHTIAVLPGDGIGREVMPEALKVLDRVGQIFDIGFRFSHLDWSCETWLATGSMMPPDGMDRLSQHDAILLGAVGYPTV
PDHISLWGMLIPIRREFDQYVNLRPVRLLPGIPCPLAGRAPGDIDFWIVRENTEGEYSQIGGRFAEGSEAEGVAQTAIFT
RRGTDRIMRYAFDLATRLGRPHVSSATKSNGLFHSMPYWDERFEKMAAEYPDIRTDSHHIDILAARFVMHPDRFDVVVGS
NLFGDILSDLGPGVTGTIAVAPSANINPEKRFPSMFEPVHGSAPDIAGRAIANPIGQVWAASMMLDHLGEGEAAALVLRA
IEHMTADPSALVRTADIGGHASTSACGDAIVAALDRIA
>Mature_357_residues
TQHTIAVLPGDGIGREVMPEALKVLDRVGQIFDIGFRFSHLDWSCETWLATGSMMPPDGMDRLSQHDAILLGAVGYPTVP
DHISLWGMLIPIRREFDQYVNLRPVRLLPGIPCPLAGRAPGDIDFWIVRENTEGEYSQIGGRFAEGSEAEGVAQTAIFTR
RGTDRIMRYAFDLATRLGRPHVSSATKSNGLFHSMPYWDERFEKMAAEYPDIRTDSHHIDILAARFVMHPDRFDVVVGSN
LFGDILSDLGPGVTGTIAVAPSANINPEKRFPSMFEPVHGSAPDIAGRAIANPIGQVWAASMMLDHLGEGEAAALVLRAI
EHMTADPSALVRTADIGGHASTSACGDAIVAALDRIA

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=32.2766570605187, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI28178816, Length=353, Percent_Identity=29.7450424929178, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=29.4797687861272, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178821, Length=354, Percent_Identity=28.8135593220339, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178838, Length=323, Percent_Identity=29.7213622291022, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178819, Length=204, Percent_Identity=32.3529411764706, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1788101, Length=363, Percent_Identity=60.3305785123967, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=39.8891966759003, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=27.75, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71986051, Length=341, Percent_Identity=31.9648093841642, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=349, Percent_Identity=28.9398280802292, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=29.0858725761773, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=366, Percent_Identity=28.9617486338798, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322097, Length=362, Percent_Identity=34.2541436464088, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=363, Percent_Identity=31.9559228650138, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319830, Length=373, Percent_Identity=31.3672922252011, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=361, Percent_Identity=30.1939058171745, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24643270, Length=350, Percent_Identity=31.1428571428571, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24643268, Length=350, Percent_Identity=31.1428571428571, Blast_Score=161, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24661184, Length=356, Percent_Identity=30.0561797752809, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI161078635, Length=354, Percent_Identity=27.9661016949153, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078637, Length=354, Percent_Identity=27.9661016949153, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078633, Length=354, Percent_Identity=27.9661016949153, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24650122, Length=354, Percent_Identity=27.9661016949153, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078639, Length=349, Percent_Identity=28.080229226361, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=26.4204545454545, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=26.4204545454545, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20130355, Length=268, Percent_Identity=28.3582089552239, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 38905; Mature: 38774

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHTIAVLPGDGIGREVMPEALKVLDRVGQIFDIGFRFSHLDWSCETWLATGSMMPPDG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCCCCCCH
MDRLSQHDAILLGAVGYPTVPDHISLWGMLIPIRREFDQYVNLRPVRLLPGIPCPLAGRA
HHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PGDIDFWIVRENTEGEYSQIGGRFAEGSEAEGVAQTAIFTRRGTDRIMRYAFDLATRLGR
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PHVSSATKSNGLFHSMPYWDERFEKMAAEYPDIRTDSHHIDILAARFVMHPDRFDVVVGS
CCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
NLFGDILSDLGPGVTGTIAVAPSANINPEKRFPSMFEPVHGSAPDIAGRAIANPIGQVWA
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASMMLDHLGEGEAAALVLRAIEHMTADPSALVRTADIGGHASTSACGDAIVAALDRIA
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQHTIAVLPGDGIGREVMPEALKVLDRVGQIFDIGFRFSHLDWSCETWLATGSMMPPDG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCCCCCCH
MDRLSQHDAILLGAVGYPTVPDHISLWGMLIPIRREFDQYVNLRPVRLLPGIPCPLAGRA
HHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PGDIDFWIVRENTEGEYSQIGGRFAEGSEAEGVAQTAIFTRRGTDRIMRYAFDLATRLGR
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC
PHVSSATKSNGLFHSMPYWDERFEKMAAEYPDIRTDSHHIDILAARFVMHPDRFDVVVGS
CCCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
NLFGDILSDLGPGVTGTIAVAPSANINPEKRFPSMFEPVHGSAPDIAGRAIANPIGQVWA
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHH
ASMMLDHLGEGEAAALVLRAIEHMTADPSALVRTADIGGHASTSACGDAIVAALDRIA
HHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]