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The map label for this gene is emrB [H]

Identifier: 162146177

GI number: 162146177

Start: 360818

End: 362215

Strand: Direct

Name: emrB [H]

Synonym: GDI_0349

Alternate gene names: 162146177

Gene position: 360818-362215 (Clockwise)

Preceding gene: 162146176

Following gene: 162146179

Centisome position: 9.15

GC content: 73.32

Gene sequence:

>1398_bases
ATGAACCTACCCCCGGCCGACCGGCTCGCCCCCGGCGCGTGGCGCGTGGTGTGCGTCGCCGCCATCGGGTCGTTCATGGC
GCAGCTCGACGCCACGGTCGTCAACGTGTCGCTGGCGCCGCTGGCCGTGGAGCTGCATGCTGGCCTGCCGGCCATCCAGT
GGGTGATGAGCGGCTATCTGCTCGCCCTGGCGCTGACGCTGCCGCTGAACGGCTGGCTGGTGGACCGCATCGGCGCCCGG
GCGCTCTATCTGTGGTGCTTCGCGGCCTTCACGCTTTCCTCGGGGCTGTGCGGGCTGGCCTGGTCGGCCCCGTCGCTGGT
TGCCTTCCGTGTCCTGCAGGGCATGGCCGGCGGGCTGCTGGCCCCCATGGCGCAGATGATGGTCGCCCGCGTCGCCGGCC
GGAACATGGCGCAGGTGGCCGGCATCGCCGCCCTGCCGATCCTGCTGGCGCCGCTGCTGGGGCCGGTCGCCGCGGGCGCG
ATCCTGCATTTCGCCTCATGGCGCTGGCTCTTCCTCGTGAACCTGCCGGTCGGGGGGCTGGCGCTGGCGCTGGCGGCGAC
GGTCCTGCCCGACGACCGGCAGGACACGCGGCCGCGCGGCCTCGACCTGACCGGGCTGGCCCTGCTGTCGCCGGGTCTGG
TCCTGTTCCTCTACGGTTCGGACCATGCGTGCACGCGGACCGGCGGCGCGGCCCTGGCCGTCTCCGTCATCCTGTTCGCG
CTCTATTACCGCGCGGCGCGGGCCCGGGGCGACGCGGCGCTGATCGATTTGCGCCTGTTCGGGGGCCGGAACTTCTCCGC
CGCGATCGTCGCCCTGTTCATGCTCAACGGCATTTCCTATGCCGGGCAGATGCTGGTTCCGATCTATCTGGTCCGGGCCT
GCGGGCTGTCGCCCGGCCAGACCGGCTGGCTGATGGCGCCGCTGGGGCTGGGCATGATGTGCACCTATCCGTTCATGGGT
CGGCTGACGAAACGCTTCGGCATCCGCCGGCTGGCGGCCGGCGGCGCGCTGCTGGCCCTGGCCGGGACGCTGCCGCTGAT
CGTCCTGGCGCGCCACGGGTTCGCCGCCGCCGTGCTGGCGCCCGGCCTGTTCCTGCGGGGCATCGGCATCAGCGCCATCG
GCATCCCGTCCATGACCTCGGGCTATGCCGCGGTGGCGCGGCGGGACCTGCCGATGGCCACCACCGCCATGAACATCGTC
CAGCGCCTGGGCGGCCCGATGCTGACGACGCTGTGCGCGACCTTCCTGGGGTGGCGCCTGTCCGCCACGGCCGGCGCGGC
GACGGTTCCGGGCGCCTTTGCCGCGTCGTTCGGCCTGCTGTGCATCCTGCACGCGCTGCTGGTCCTGGCGGCGTTGCGGC
TGCCCCGGCGGCTTCCCGGGGCCGGACCCGCGCCGTGA

Upstream 100 bases:

>100_bases
ACCTCAGCCCCGAGGCGCACGACAACAGCGTCCAGCGGATCTTCCCGCGACTGGGCGAGACCGGGTCCACCCGGCAGGTC
ATCGACCTTCTCGAACGCGG

Downstream 100 bases:

>100_bases
TCGCCCCCTATCCGGACGGCGCGCCCCACGCGGGGGCGTTCCACCGGATCTGCCGCTCGGTCCCGATGTCCGGCGGCCCG
ATGTCCGGCGGGCTGACCGG

Product: drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNLPPADRLAPGAWRVVCVAAIGSFMAQLDATVVNVSLAPLAVELHAGLPAIQWVMSGYLLALALTLPLNGWLVDRIGAR
ALYLWCFAAFTLSSGLCGLAWSAPSLVAFRVLQGMAGGLLAPMAQMMVARVAGRNMAQVAGIAALPILLAPLLGPVAAGA
ILHFASWRWLFLVNLPVGGLALALAATVLPDDRQDTRPRGLDLTGLALLSPGLVLFLYGSDHACTRTGGAALAVSVILFA
LYYRAARARGDAALIDLRLFGGRNFSAAIVALFMLNGISYAGQMLVPIYLVRACGLSPGQTGWLMAPLGLGMMCTYPFMG
RLTKRFGIRRLAAGGALLALAGTLPLIVLARHGFAAAVLAPGLFLRGIGISAIGIPSMTSGYAAVARRDLPMATTAMNIV
QRLGGPMLTTLCATFLGWRLSATAGAATVPGAFAASFGLLCILHALLVLAALRLPRRLPGAGPAP

Sequences:

>Translated_465_residues
MNLPPADRLAPGAWRVVCVAAIGSFMAQLDATVVNVSLAPLAVELHAGLPAIQWVMSGYLLALALTLPLNGWLVDRIGAR
ALYLWCFAAFTLSSGLCGLAWSAPSLVAFRVLQGMAGGLLAPMAQMMVARVAGRNMAQVAGIAALPILLAPLLGPVAAGA
ILHFASWRWLFLVNLPVGGLALALAATVLPDDRQDTRPRGLDLTGLALLSPGLVLFLYGSDHACTRTGGAALAVSVILFA
LYYRAARARGDAALIDLRLFGGRNFSAAIVALFMLNGISYAGQMLVPIYLVRACGLSPGQTGWLMAPLGLGMMCTYPFMG
RLTKRFGIRRLAAGGALLALAGTLPLIVLARHGFAAAVLAPGLFLRGIGISAIGIPSMTSGYAAVARRDLPMATTAMNIV
QRLGGPMLTTLCATFLGWRLSATAGAATVPGAFAASFGLLCILHALLVLAALRLPRRLPGAGPAP
>Mature_465_residues
MNLPPADRLAPGAWRVVCVAAIGSFMAQLDATVVNVSLAPLAVELHAGLPAIQWVMSGYLLALALTLPLNGWLVDRIGAR
ALYLWCFAAFTLSSGLCGLAWSAPSLVAFRVLQGMAGGLLAPMAQMMVARVAGRNMAQVAGIAALPILLAPLLGPVAAGA
ILHFASWRWLFLVNLPVGGLALALAATVLPDDRQDTRPRGLDLTGLALLSPGLVLFLYGSDHACTRTGGAALAVSVILFA
LYYRAARARGDAALIDLRLFGGRNFSAAIVALFMLNGISYAGQMLVPIYLVRACGLSPGQTGWLMAPLGLGMMCTYPFMG
RLTKRFGIRRLAAGGALLALAGTLPLIVLARHGFAAAVLAPGLFLRGIGISAIGIPSMTSGYAAVARRDLPMATTAMNIV
QRLGGPMLTTLCATFLGWRLSATAGAATVPGAFAASFGLLCILHALLVLAALRLPRRLPGAGPAP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=322, Percent_Identity=34.1614906832298, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789042, Length=418, Percent_Identity=26.555023923445, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=30.4455445544554, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788710, Length=417, Percent_Identity=26.8585131894484, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081983, Length=352, Percent_Identity=31.25, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48175; Mature: 48175

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPPADRLAPGAWRVVCVAAIGSFMAQLDATVVNVSLAPLAVELHAGLPAIQWVMSGYL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHEEEEHHCCCHHHHHHHHHHH
LALALTLPLNGWLVDRIGARALYLWCFAAFTLSSGLCGLAWSAPSLVAFRVLQGMAGGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHH
APMAQMMVARVAGRNMAQVAGIAALPILLAPLLGPVAAGAILHFASWRWLFLVNLPVGGL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
ALALAATVLPDDRQDTRPRGLDLTGLALLSPGLVLFLYGSDHACTRTGGAALAVSVILFA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
LYYRAARARGDAALIDLRLFGGRNFSAAIVALFMLNGISYAGQMLVPIYLVRACGLSPGQ
HHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGWLMAPLGLGMMCTYPFMGRLTKRFGIRRLAAGGALLALAGTLPLIVLARHGFAAAVLA
CCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
PGLFLRGIGISAIGIPSMTSGYAAVARRDLPMATTAMNIVQRLGGPMLTTLCATFLGWRL
CHHHHHCCCCCEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
SATAGAATVPGAFAASFGLLCILHALLVLAALRLPRRLPGAGPAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLPPADRLAPGAWRVVCVAAIGSFMAQLDATVVNVSLAPLAVELHAGLPAIQWVMSGYL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHEEEEHHCCCHHHHHHHHHHH
LALALTLPLNGWLVDRIGARALYLWCFAAFTLSSGLCGLAWSAPSLVAFRVLQGMAGGLL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCHHH
APMAQMMVARVAGRNMAQVAGIAALPILLAPLLGPVAAGAILHFASWRWLFLVNLPVGGL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
ALALAATVLPDDRQDTRPRGLDLTGLALLSPGLVLFLYGSDHACTRTGGAALAVSVILFA
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
LYYRAARARGDAALIDLRLFGGRNFSAAIVALFMLNGISYAGQMLVPIYLVRACGLSPGQ
HHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGWLMAPLGLGMMCTYPFMGRLTKRFGIRRLAAGGALLALAGTLPLIVLARHGFAAAVLA
CCCEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
PGLFLRGIGISAIGIPSMTSGYAAVARRDLPMATTAMNIVQRLGGPMLTTLCATFLGWRL
CHHHHHCCCCCEECCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
SATAGAATVPGAFAASFGLLCILHALLVLAALRLPRRLPGAGPAP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]