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The map label for this gene is eutB [H]

Identifier: 162146138

GI number: 162146138

Start: 318548

End: 320038

Strand: Reverse

Name: eutB [H]

Synonym: GDI_0310

Alternate gene names: 162146138

Gene position: 320038-318548 (Counterclockwise)

Preceding gene: 162146141

Following gene: 162146137

Centisome position: 8.11

GC content: 67.0

Gene sequence:

>1491_bases
GTGCCTTTATCAGCCCGCGATAAACTCCTGTCCGGCAACCAGTCTCATTCTCTTCAAATATCTGAACGGGCTTCGCTATT
CGGAAGACAAAAGAGAAGGATCATCCCCACCGTGCCCTATCGGATCATCCTGGACAGCGAACGCTACAATTTCGCCCATC
TGCGCCACGTCCTGGCCTGCGCGTCCCCGGCGCGGTCGGGCGACGAACTGGCCGGCCTGGCCGCCGCCGACCCGGTGGAA
CGCATGGCCGCCCGGCATGTCCTGGCCGACATTCCGCTGCGCGATTTCCTGAACGATGTGCCGGTACCCTACGAGGATGA
CGAGGTCACGCGGCTGATCGTCGATACGCACGACGCGGCGTCCTTCGCGCCGGTCGCGCACATGACGGTCGGGCAGTTCC
GCGACTGGCTGCTGTCCTACACGGCCGATGGCGACGCGATCACGGCGCTGGGGCCGGGCCTGACGCCGGAAATCGTGTCG
GCCGTCAGCAAGCTGATGCGCAACCAGGACCTGATCGCGGCGGCGCGCAAGATCCGGGTGGTGACGGCGTTCCGCAACAC
GCAGGGCCTGCCCGGCCGGCTGGGCACGCGGTTGCAGCCCAACCACCCGACCGACGATGCGCGCGGCATCGCGGCGTCGC
TTCTGGACGGGCTGCTGCTGGGGGTGGGCGACGCCGTGCTGGGCATCAACCCCGCCACCGACAGCATCGCCAACGGCATC
GTGCTGCTGGACATGCTGGAACAGGTGCGCACCCGCTATGCCATCCCGGCCCAGACCTGCGTGCTGACGCACATCACCAA
CGCCATCGCGATGATGGCGCGCGGGGCACCGGTCGACCTGGTGTTCCAATCCGTGGCGGGCACCCAGGCCGCCAATGCCG
GCTTCGGCGTGTCGCTGTCCGTGCTGGCCGAGGCCCATGACGCGGCCCGGTCGCTGAATCGCGGCACGGTGGGGCACAAC
GTGATGTATTTCGAGACCGGACAGGGCTCCGCGCTGTCGGCCGGCGCCCATCACGGCATGGACCAGCAGACGGCCGAGGT
CCGGGCCTATGCCGTCGCGCGCGCGTTCTCGCCCCTGCTGGTGAACACGGTGGTGGGATTCATCGGGCCGGAATACCTGT
ATGACGGCAAGCAGATCATCCGCGCGGGGCTGGAAGACCATTTCTGCGGCAAGCTGATGGGCCTGCCGATGGGATGCGAC
GCGTGCTACACCAACCATGCCGAAGCCGACCAGGACGACATGGACAACCTGATGATGCTGCTGACGGTGGCGGGGGTGAA
TTTCCTGATCGCCGTGCCGGGCGGCGACGACGTGATGCTGAGCTACCAGAGCCTGTCCCATCACGACATGCTGACCCTGC
GATCCAGCTTCGGCGTGCGCGCCGCGCCGGAATTCGAGGCCTGGCTGCATGAAATGAACTTGCTGGACCCCGACGGGCGG
CTGCGCGTCAATGCCCCGCAGAGCCTGGGGCGTATCCTGGGGCCGGGCTGA

Upstream 100 bases:

>100_bases
CAGACATGACAATCGTGCTGCACCCGTCATCGCGCCCATCATTTTCGAGCCCGTTCCCGCCGGAATTTCCATTCAATTCA
TTGTCGATATTCGGGGTTCC

Downstream 100 bases:

>100_bases
CATTTCAGAGGCTGTTTCAAAAGCCTCTCGGACCGGACCGGCAACCCCGCCCCCCGCAAAACGCTGGAAAACCAGTATAC
TGCGAGAGGCACCCGACGAT

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MPLSARDKLLSGNQSHSLQISERASLFGRQKRRIIPTVPYRIILDSERYNFAHLRHVLACASPARSGDELAGLAAADPVE
RMAARHVLADIPLRDFLNDVPVPYEDDEVTRLIVDTHDAASFAPVAHMTVGQFRDWLLSYTADGDAITALGPGLTPEIVS
AVSKLMRNQDLIAAARKIRVVTAFRNTQGLPGRLGTRLQPNHPTDDARGIAASLLDGLLLGVGDAVLGINPATDSIANGI
VLLDMLEQVRTRYAIPAQTCVLTHITNAIAMMARGAPVDLVFQSVAGTQAANAGFGVSLSVLAEAHDAARSLNRGTVGHN
VMYFETGQGSALSAGAHHGMDQQTAEVRAYAVARAFSPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLMGLPMGCD
ACYTNHAEADQDDMDNLMMLLTVAGVNFLIAVPGGDDVMLSYQSLSHHDMLTLRSSFGVRAAPEFEAWLHEMNLLDPDGR
LRVNAPQSLGRILGPG

Sequences:

>Translated_496_residues
MPLSARDKLLSGNQSHSLQISERASLFGRQKRRIIPTVPYRIILDSERYNFAHLRHVLACASPARSGDELAGLAAADPVE
RMAARHVLADIPLRDFLNDVPVPYEDDEVTRLIVDTHDAASFAPVAHMTVGQFRDWLLSYTADGDAITALGPGLTPEIVS
AVSKLMRNQDLIAAARKIRVVTAFRNTQGLPGRLGTRLQPNHPTDDARGIAASLLDGLLLGVGDAVLGINPATDSIANGI
VLLDMLEQVRTRYAIPAQTCVLTHITNAIAMMARGAPVDLVFQSVAGTQAANAGFGVSLSVLAEAHDAARSLNRGTVGHN
VMYFETGQGSALSAGAHHGMDQQTAEVRAYAVARAFSPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLMGLPMGCD
ACYTNHAEADQDDMDNLMMLLTVAGVNFLIAVPGGDDVMLSYQSLSHHDMLTLRSSFGVRAAPEFEAWLHEMNLLDPDGR
LRVNAPQSLGRILGPG
>Mature_495_residues
PLSARDKLLSGNQSHSLQISERASLFGRQKRRIIPTVPYRIILDSERYNFAHLRHVLACASPARSGDELAGLAAADPVER
MAARHVLADIPLRDFLNDVPVPYEDDEVTRLIVDTHDAASFAPVAHMTVGQFRDWLLSYTADGDAITALGPGLTPEIVSA
VSKLMRNQDLIAAARKIRVVTAFRNTQGLPGRLGTRLQPNHPTDDARGIAASLLDGLLLGVGDAVLGINPATDSIANGIV
LLDMLEQVRTRYAIPAQTCVLTHITNAIAMMARGAPVDLVFQSVAGTQAANAGFGVSLSVLAEAHDAARSLNRGTVGHNV
MYFETGQGSALSAGAHHGMDQQTAEVRAYAVARAFSPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLMGLPMGCDA
CYTNHAEADQDDMDNLMMLLTVAGVNFLIAVPGGDDVMLSYQSLSHHDMLTLRSSFGVRAAPEFEAWLHEMNLLDPDGRL
RVNAPQSLGRILGPG

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=433, Percent_Identity=48.729792147806, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 53250; Mature: 53118

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSARDKLLSGNQSHSLQISERASLFGRQKRRIIPTVPYRIILDSERYNFAHLRHVLAC
CCCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
ASPARSGDELAGLAAADPVERMAARHVLADIPLRDFLNDVPVPYEDDEVTRLIVDTHDAA
CCCCCCCCHHHCCHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEEECCCCC
SFAPVAHMTVGQFRDWLLSYTADGDAITALGPGLTPEIVSAVSKLMRNQDLIAAARKIRV
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHEE
VTAFRNTQGLPGRLGTRLQPNHPTDDARGIAASLLDGLLLGVGDAVLGINPATDSIANGI
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHCCH
VLLDMLEQVRTRYAIPAQTCVLTHITNAIAMMARGAPVDLVFQSVAGTQAANAGFGVSLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHH
VLAEAHDAARSLNRGTVGHNVMYFETGQGSALSAGAHHGMDQQTAEVRAYAVARAFSPLL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHH
VNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLMGLPMGCDACYTNHAEADQDDMDNLMML
HHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHH
LTVAGVNFLIAVPGGDDVMLSYQSLSHHDMLTLRSSFGVRAAPEFEAWLHEMNLLDPDGR
HHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC
LRVNAPQSLGRILGPG
EEECCHHHHHHHCCCC
>Mature Secondary Structure 
PLSARDKLLSGNQSHSLQISERASLFGRQKRRIIPTVPYRIILDSERYNFAHLRHVLAC
CCCCHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
ASPARSGDELAGLAAADPVERMAARHVLADIPLRDFLNDVPVPYEDDEVTRLIVDTHDAA
CCCCCCCCHHHCCHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEEECCCCC
SFAPVAHMTVGQFRDWLLSYTADGDAITALGPGLTPEIVSAVSKLMRNQDLIAAARKIRV
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHEE
VTAFRNTQGLPGRLGTRLQPNHPTDDARGIAASLLDGLLLGVGDAVLGINPATDSIANGI
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHCCH
VLLDMLEQVRTRYAIPAQTCVLTHITNAIAMMARGAPVDLVFQSVAGTQAANAGFGVSLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHH
VLAEAHDAARSLNRGTVGHNVMYFETGQGSALSAGAHHGMDQQTAEVRAYAVARAFSPLL
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEECCCHHCCCCHHHHHHHHHHHHHHHHHHH
VNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLMGLPMGCDACYTNHAEADQDDMDNLMML
HHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHH
LTVAGVNFLIAVPGGDDVMLSYQSLSHHDMLTLRSSFGVRAAPEFEAWLHEMNLLDPDGR
HHHHCCEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC
LRVNAPQSLGRILGPG
EEECCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]