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The map label for this gene is cysG

Identifier: 162145997

GI number: 162145997

Start: 173540

End: 174952

Strand: Reverse

Name: cysG

Synonym: GDI_0165

Alternate gene names: 162145997

Gene position: 174952-173540 (Counterclockwise)

Preceding gene: 162145998

Following gene: 162145995

Centisome position: 4.44

GC content: 74.66

Gene sequence:

>1413_bases
ATGAGCGATGCGACCGACCCCGGCTGGTTCCCGCTGGCGCTGCGGCTGCGCGGCGCGCGGGTGGTGGTGGTGGGCGGCGG
CGGGATCGCGCTGAACAAGGTCCGGCTGCTGCTGGCCCACGCCGCGCGGATCGACATCCTGGCCCCCCGGCTGGAGGACA
CGCTGGCCGCCTGGCAGGCCGAAGGGCGGATCACCCACATCGCGGGCGAGGCGACGCCCGACCGGGTGCGTGCGCTGCTG
CCCGGCAGCCGCCTGGTCTATGCCGCGACCGACGACCGGGCGGTGAACCGCGCCGTCGCGGCGCAGGCCGATGCGCTGAA
TATCCCGGTCTGCGCGGTGGACGACCCGGAGCCGTCTTCCTTCATCACGCCCGCGCAGATCCATCGCGGGCCGGTGCGGA
TCGCGATTTCCACCGGCGGCGCGGCCCCGGTGCTGGCCCGGCGCCTGCGCGAGCGGATCGAGGCCGTGATGCCGGCAGGG
CTCGACGCGCTGGCGCGCTTCCTGCAGGCCGAGCGCGCTCATGTCGTGGCTGCCTGCCCCGATATCGGCCGCCGCCGCCG
GGTATGGGAGGATTTCCTGGACGGCCCAGGCGGCGAGGCGGCGCAGCGCGGCGAACACGCGGCCGCGCGACAGGTACTGG
ACCACCTGCTGGCCGGCGCGCAGACCGGGGGCGAGGTCTGGCTGGTCGGCGCCGGGCCGGGGGACCCGGACCTGCTGACC
CTGCGGGCGCTGCACCTGATGCAGAACGCGGATTCGGTGCTGTACGACCAGTTGCTGCCGCCCGCGTTGATGGACCGGGT
GCGCCGCGATGCCGAGCGGGTGTTCGTGGGCAAGCAGCGCGACCGCCACACCATGCCGCAGGACGACATCAATGCCGAAC
TGATCCGCCGCGCGCGGGCGGGCGAGCGGGTGCTGCGCCTGAAGGGCGGGGACCCGTTCATCTTCGGTCGCGGCGGCGAG
GAGATCGAGGCCCTGATGGCGGCGGGAATTCCGTTCCAGGTCGTGCCGGGCATCACGGCGGCCAGCGGCTGCGCCGCCTA
TGCCGGCATTCCGCTGACCCACCGGGACTGCGCCCAGTCCTGCCTGTTCGTCACCGGTCACGCCCGCCGCGACGGCACGC
TGGACCTGCCGTGGGACAGCATGGCCCGGCCGGGGCAGACCATCGCGATCTATATGGGCGTCACCGCGCTGCCGGACCTG
TGCACCATGCTGGTGCGCCACGGCCTGCCGCCCGACTGGCCCGCCGCCGTGGTGGAGCGCGGCACCCGGCCCGACCAGCG
CGTGTTGACGGGAACCCTGGCCGACCTGCCGGCGCTGGCGCGCGCCCATGCCGTGGGCAGCCCGGCGCTGGTGCTGGTGG
GCCAGGTGGTGCGCCACCGCGTCGTCACGCCGCCGCCCCTGTCCGGTACGTGA

Upstream 100 bases:

>100_bases
TGCCGCCGGGCCTGGCGCGGCTGGACCTGGCGGATACCGCCGCCATCACCGATTTCATCCTGGCGCGGGCCTGCCCCCTG
CCCGCCCCGGCAGGTGCGGC

Downstream 100 bases:

>100_bases
AAGGCTGCCTCGCAGGCATCCTACGAGATGTCGGCCATCAGCGAGACGTGGGCGCAGGTGATCTTCCAGCCGGCCTCGGT
CCGCAGCCAGGTCTGGCTCT

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDATDPGWFPLALRLRGARVVVVGGGGIALNKVRLLLAHAARIDILAPRLEDTLAAWQAEGRITHIAGEATPDRVRALL
PGSRLVYAATDDRAVNRAVAAQADALNIPVCAVDDPEPSSFITPAQIHRGPVRIAISTGGAAPVLARRLRERIEAVMPAG
LDALARFLQAERAHVVAACPDIGRRRRVWEDFLDGPGGEAAQRGEHAAARQVLDHLLAGAQTGGEVWLVGAGPGDPDLLT
LRALHLMQNADSVLYDQLLPPALMDRVRRDAERVFVGKQRDRHTMPQDDINAELIRRARAGERVLRLKGGDPFIFGRGGE
EIEALMAAGIPFQVVPGITAASGCAAYAGIPLTHRDCAQSCLFVTGHARRDGTLDLPWDSMARPGQTIAIYMGVTALPDL
CTMLVRHGLPPDWPAAVVERGTRPDQRVLTGTLADLPALARAHAVGSPALVLVGQVVRHRVVTPPPLSGT

Sequences:

>Translated_470_residues
MSDATDPGWFPLALRLRGARVVVVGGGGIALNKVRLLLAHAARIDILAPRLEDTLAAWQAEGRITHIAGEATPDRVRALL
PGSRLVYAATDDRAVNRAVAAQADALNIPVCAVDDPEPSSFITPAQIHRGPVRIAISTGGAAPVLARRLRERIEAVMPAG
LDALARFLQAERAHVVAACPDIGRRRRVWEDFLDGPGGEAAQRGEHAAARQVLDHLLAGAQTGGEVWLVGAGPGDPDLLT
LRALHLMQNADSVLYDQLLPPALMDRVRRDAERVFVGKQRDRHTMPQDDINAELIRRARAGERVLRLKGGDPFIFGRGGE
EIEALMAAGIPFQVVPGITAASGCAAYAGIPLTHRDCAQSCLFVTGHARRDGTLDLPWDSMARPGQTIAIYMGVTALPDL
CTMLVRHGLPPDWPAAVVERGTRPDQRVLTGTLADLPALARAHAVGSPALVLVGQVVRHRVVTPPPLSGT
>Mature_469_residues
SDATDPGWFPLALRLRGARVVVVGGGGIALNKVRLLLAHAARIDILAPRLEDTLAAWQAEGRITHIAGEATPDRVRALLP
GSRLVYAATDDRAVNRAVAAQADALNIPVCAVDDPEPSSFITPAQIHRGPVRIAISTGGAAPVLARRLRERIEAVMPAGL
DALARFLQAERAHVVAACPDIGRRRRVWEDFLDGPGGEAAQRGEHAAARQVLDHLLAGAQTGGEVWLVGAGPGDPDLLTL
RALHLMQNADSVLYDQLLPPALMDRVRRDAERVFVGKQRDRHTMPQDDINAELIRRARAGERVLRLKGGDPFIFGRGGEE
IEALMAAGIPFQVVPGITAASGCAAYAGIPLTHRDCAQSCLFVTGHARRDGTLDLPWDSMARPGQTIAIYMGVTALPDLC
TMLVRHGLPPDWPAAVVERGTRPDQRVLTGTLADLPALARAHAVGSPALVLVGQVVRHRVVTPPPLSGT

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=450, Percent_Identity=45.1111111111111, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6322922, Length=240, Percent_Identity=35.4166666666667, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG_GLUDA (A9H259)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600455.1
- RefSeq:   YP_002276605.1
- ProteinModelPortal:   A9H259
- SMR:   A9H259
- GeneID:   5788693
- GeneID:   6975664
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_2235
- HOGENOM:   HBG730212
- OMA:   RITHIAG
- ProtClustDB:   CLSK936614
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 50124; Mature: 49993

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDATDPGWFPLALRLRGARVVVVGGGGIALNKVRLLLAHAARIDILAPRLEDTLAAWQA
CCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EGRITHIAGEATPDRVRALLPGSRLVYAATDDRAVNRAVAAQADALNIPVCAVDDPEPSS
CCCEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEEECCCCCCCC
FITPAQIHRGPVRIAISTGGAAPVLARRLRERIEAVMPAGLDALARFLQAERAHVVAACP
CCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEECC
DIGRRRRVWEDFLDGPGGEAAQRGEHAAARQVLDHLLAGAQTGGEVWLVGAGPGDPDLLT
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
LRALHLMQNADSVLYDQLLPPALMDRVRRDAERVFVGKQRDRHTMPQDDINAELIRRARA
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC
GERVLRLKGGDPFIFGRGGEEIEALMAAGIPFQVVPGITAASGCAAYAGIPLTHRDCAQS
CCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCHHHHHHH
CLFVTGHARRDGTLDLPWDSMARPGQTIAIYMGVTALPDLCTMLVRHGLPPDWPAAVVER
EEEEECCCCCCCCEECCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHC
GTRPDQRVLTGTLADLPALARAHAVGSPALVLVGQVVRHRVVTPPPLSGT
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDATDPGWFPLALRLRGARVVVVGGGGIALNKVRLLLAHAARIDILAPRLEDTLAAWQA
CCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
EGRITHIAGEATPDRVRALLPGSRLVYAATDDRAVNRAVAAQADALNIPVCAVDDPEPSS
CCCEEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEEECCCCCCCC
FITPAQIHRGPVRIAISTGGAAPVLARRLRERIEAVMPAGLDALARFLQAERAHVVAACP
CCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEECC
DIGRRRRVWEDFLDGPGGEAAQRGEHAAARQVLDHLLAGAQTGGEVWLVGAGPGDPDLLT
CCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHH
LRALHLMQNADSVLYDQLLPPALMDRVRRDAERVFVGKQRDRHTMPQDDINAELIRRARA
HHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHC
GERVLRLKGGDPFIFGRGGEEIEALMAAGIPFQVVPGITAASGCAAYAGIPLTHRDCAQS
CCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECCCCCHHCCCHHHCCCCCCHHHHHHH
CLFVTGHARRDGTLDLPWDSMARPGQTIAIYMGVTALPDLCTMLVRHGLPPDWPAAVVER
EEEEECCCCCCCCEECCHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHC
GTRPDQRVLTGTLADLPALARAHAVGSPALVLVGQVVRHRVVTPPPLSGT
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA