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The map label for this gene is ycdT [H]

Identifier: 162145995

GI number: 162145995

Start: 171697

End: 172869

Strand: Reverse

Name: ycdT [H]

Synonym: GDI_0163

Alternate gene names: 162145995

Gene position: 172869-171697 (Counterclockwise)

Preceding gene: 162145997

Following gene: 162145993

Centisome position: 4.38

GC content: 65.47

Gene sequence:

>1173_bases
ATGCAGGCTGGCCTATTGACCCTCTATTGCCTGGCGATCGGCACGCTCGTCATCGGATGCGGCGCGACGCTGTGGGAGCT
TCACGAACAGCCCGGCCGGCGCGTCCCGCTCCTCTGCTGGGCGGCGGCCTATGCATGTTATGCGATCGGGTGCGTCATCA
CCCTCCTGCGGCCGGACCTGCCCGGTGTTTCCGGATGGGCCGTCAGCAACGTGCTGTTCACGGCAGGCTATGCCCTGGTC
CTGAACGGGGTCGGGCAACTCGATCATCGGCGATACGTCGCGACTTCGATCACCCTGCTCGTCGCCGTGTCCCTCGTGTG
GATCATCGGCGGCCCCGCGATATGCCGGATCATGTGGGATTATGGCGCCGCGTTCCCCATCGCCCTGATGTGCGGCCTCA
CGGCCTGGCATATCCAGTATCGGTCCGGCATCCAGGCCCCGCGTTCGCGGCCGGTGGTCGCGGCGATCGCGTGGGGACAC
GGGGTGTTCTATCTGTTCCGCGGCGCCGTCATGCCCCTTATGGTCCTTTGGAAGGGACAGCATGCTTCCTCGGTTTTTGC
CGAGGTCACGATGTACGAGGGCGTTCTGTATTCCGTTGCGATGCCGGTCGCGCTGCTGTCCGTGGTCAGGGAGGAGAGCC
GCCTCGCCATGCTGAAAATCGCACAGACGGACTTCCTGACGAATATCGGAAACCGCCATTTTTTCTATCAGGCCGGCCAG
AAATGCCTGTCCGGCCAGACGGTCGGCCAGCCGGTTTCCCTGCTGATCTTCGACATAGACCATTTCAAGTCCATCAATGA
CATCCACGGTCACGCAATGGGCGACGCCGTGCTGAAGCTCTTCGCCCGTGCCATGGGCGAGGAGGTGGGCCAGGAGGCGA
TCATGGGCCGCCTGGGCGGCGAGGAGTTCGCGATGCTGCTGCCGGGAAGCGACAGGGCCACGGCGCGTGCGGCAGGCCAG
AATATCGCACGGCGCTTCACCGCCCTCGCGGCTCAGGACTTCCGGCCGCCCCTGCACGTGACGGTGAGCGTCGGCCTGAC
GGAGGTCCGGACCAGCCGGATGACGCTGGACGACCTTCTGGCCGCGGCAGACGGCGCCCTGTACCAGGCGAAGACCCTCG
GTCGAAACCGCATCGAATTCGCCAGCGCGGCCGCCTTCCACCCAGCGGCGTAG

Upstream 100 bases:

>100_bases
CCCGCAGACAGGCCGCTCCCTTACAGTGAAGGCCTTCGCATGAACCTGGTCATGCGAGGGTTGTACCGAAATTTCGCACC
GTGATACCCGGAAAGCATCC

Downstream 100 bases:

>100_bases
CGCAACCCGGGCCGCATCGATGTTTCAGCGCGCGGCGAGGCTTCGCTTCAGGCGGGTGATGGCGGCGGGCAGGGCGCGGA
CCTTCAGCACGCGGCCGGTC

Product: GGDEF family signaling protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQAGLLTLYCLAIGTLVIGCGATLWELHEQPGRRVPLLCWAAAYACYAIGCVITLLRPDLPGVSGWAVSNVLFTAGYALV
LNGVGQLDHRRYVATSITLLVAVSLVWIIGGPAICRIMWDYGAAFPIALMCGLTAWHIQYRSGIQAPRSRPVVAAIAWGH
GVFYLFRGAVMPLMVLWKGQHASSVFAEVTMYEGVLYSVAMPVALLSVVREESRLAMLKIAQTDFLTNIGNRHFFYQAGQ
KCLSGQTVGQPVSLLIFDIDHFKSINDIHGHAMGDAVLKLFARAMGEEVGQEAIMGRLGGEEFAMLLPGSDRATARAAGQ
NIARRFTALAAQDFRPPLHVTVSVGLTEVRTSRMTLDDLLAAADGALYQAKTLGRNRIEFASAAAFHPAA

Sequences:

>Translated_390_residues
MQAGLLTLYCLAIGTLVIGCGATLWELHEQPGRRVPLLCWAAAYACYAIGCVITLLRPDLPGVSGWAVSNVLFTAGYALV
LNGVGQLDHRRYVATSITLLVAVSLVWIIGGPAICRIMWDYGAAFPIALMCGLTAWHIQYRSGIQAPRSRPVVAAIAWGH
GVFYLFRGAVMPLMVLWKGQHASSVFAEVTMYEGVLYSVAMPVALLSVVREESRLAMLKIAQTDFLTNIGNRHFFYQAGQ
KCLSGQTVGQPVSLLIFDIDHFKSINDIHGHAMGDAVLKLFARAMGEEVGQEAIMGRLGGEEFAMLLPGSDRATARAAGQ
NIARRFTALAAQDFRPPLHVTVSVGLTEVRTSRMTLDDLLAAADGALYQAKTLGRNRIEFASAAAFHPAA
>Mature_390_residues
MQAGLLTLYCLAIGTLVIGCGATLWELHEQPGRRVPLLCWAAAYACYAIGCVITLLRPDLPGVSGWAVSNVLFTAGYALV
LNGVGQLDHRRYVATSITLLVAVSLVWIIGGPAICRIMWDYGAAFPIALMCGLTAWHIQYRSGIQAPRSRPVVAAIAWGH
GVFYLFRGAVMPLMVLWKGQHASSVFAEVTMYEGVLYSVAMPVALLSVVREESRLAMLKIAQTDFLTNIGNRHFFYQAGQ
KCLSGQTVGQPVSLLIFDIDHFKSINDIHGHAMGDAVLKLFARAMGEEVGQEAIMGRLGGEEFAMLLPGSDRATARAAGQ
NIARRFTALAAQDFRPPLHVTVSVGLTEVRTSRMTLDDLLAAADGALYQAKTLGRNRIEFASAAAFHPAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=33.5294117647059, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787802, Length=164, Percent_Identity=37.8048780487805, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081881, Length=256, Percent_Identity=30.46875, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=30.8139534883721, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=33.5329341317365, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=29.6296296296296, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI145693134, Length=167, Percent_Identity=32.3353293413174, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=33.7078651685393, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=28.4883720930233, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87081977, Length=203, Percent_Identity=26.1083743842365, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=189, Percent_Identity=27.5132275132275, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=33.1288343558282, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42106; Mature: 42106

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCHHHHHHCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure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HHCCCCHHHHHHCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]