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The map label for this gene is hisF

Identifier: 162145907

GI number: 162145907

Start: 74904

End: 75680

Strand: Reverse

Name: hisF

Synonym: GDI_0069

Alternate gene names: 162145907

Gene position: 75680-74904 (Counterclockwise)

Preceding gene: 162145908

Following gene: 162145906

Centisome position: 1.92

GC content: 70.27

Gene sequence:

>777_bases
ATGCTGAAGCTGCGGGTCATCCCCTGTCTGGACGTCAAGAACGGGCGCGTCGTGAAGGGCGTCAACTTCGTATCGCTGCG
CGATGCCGGGGACCCGGTGGAGCAGGCGGCGGTCTATGATGCCGCCGGGGCGGACGAACTGACCTTCTTGGACATCACGG
CCAGCCACGAGAACCGGGACACGATCCTGGACGTGGTCAGCCGTACCGCCGAGCGGATCTTCCTGCCGCTGACGGTGGGG
GGCGGCGTGCGGACGACCGACGACATGCGGCGCCTGCTGCTGGCCGGGGCGGACAAGTGCGCGATGAATTCCGCCGCCGT
CGCCCGGCCCGACCTGGTCAGCGAGGCCGCGCGGAAATTCGGCAGCCAGTGCGTGGTGGTGGCCGTCGATGCCCGGTCGG
ACGGGCATGGATCGTGGGAGGTGTACACCCATGGCGGCCGCACGCCCACAGGGCGCAACGTCATCGACTGGTGCCGCGAG
GTGGTCGAACGCGGCGCGGGCGAGATCCTGCTGACCAGCATGGACCGCGACGGTACCGGCAGCGGGTTCGACCTGGACCT
GCTGTGCGCGGCGTGTACTGCGGTACGGGTGCCGATCGTGGCCTCGGGCGGGGTCGGCACGCTGGAGCATTTCGTCGAGG
GCGCGCGCGCGGGCGCCACCGGCCTGCTGGCCGCCAGCGTCTTTCATTTCGGCCAGTTCACCATTCCGCAGGTCAAGCAG
GCGCTGGCCGATGCCGGCCTGCCGGTGCGCCACACGCCCGCACACCCCGTACCCTGA

Upstream 100 bases:

>100_bases
CCGCGACGGTGCAGGCACCGGGGATCGAGGGCGTGATCGTCGGCCGGGCGCTGTATGACGGCCGGGTGGACCCGGCCGAG
GCCTTGCGGATCCTGTCCTG

Downstream 100 bases:

>100_bases
ACCGCGCCCCCAGCCGCGCCGGACACCCAGCAGAGGGCATGATGCCAAAGATCCTGAAGACGACCCGCAAGAGCGCCGAG
ACCAAGGCCGTGGACGACAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLKLRVIPCLDVKNGRVVKGVNFVSLRDAGDPVEQAAVYDAAGADELTFLDITASHENRDTILDVVSRTAERIFLPLTVG
GGVRTTDDMRRLLLAGADKCAMNSAAVARPDLVSEAARKFGSQCVVVAVDARSDGHGSWEVYTHGGRTPTGRNVIDWCRE
VVERGAGEILLTSMDRDGTGSGFDLDLLCAACTAVRVPIVASGGVGTLEHFVEGARAGATGLLAASVFHFGQFTIPQVKQ
ALADAGLPVRHTPAHPVP

Sequences:

>Translated_258_residues
MLKLRVIPCLDVKNGRVVKGVNFVSLRDAGDPVEQAAVYDAAGADELTFLDITASHENRDTILDVVSRTAERIFLPLTVG
GGVRTTDDMRRLLLAGADKCAMNSAAVARPDLVSEAARKFGSQCVVVAVDARSDGHGSWEVYTHGGRTPTGRNVIDWCRE
VVERGAGEILLTSMDRDGTGSGFDLDLLCAACTAVRVPIVASGGVGTLEHFVEGARAGATGLLAASVFHFGQFTIPQVKQ
ALADAGLPVRHTPAHPVP
>Mature_258_residues
MLKLRVIPCLDVKNGRVVKGVNFVSLRDAGDPVEQAAVYDAAGADELTFLDITASHENRDTILDVVSRTAERIFLPLTVG
GGVRTTDDMRRLLLAGADKCAMNSAAVARPDLVSEAARKFGSQCVVVAVDARSDGHGSWEVYTHGGRTPTGRNVIDWCRE
VVERGAGEILLTSMDRDGTGSGFDLDLLCAACTAVRVPIVASGGVGTLEHFVEGARAGATGLLAASVFHFGQFTIPQVKQ
ALADAGLPVRHTPAHPVP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=44.53125, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=30.327868852459, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=31.3099041533546, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_GLUDA (A9GZW9)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600365.1
- RefSeq:   YP_002276016.1
- ProteinModelPortal:   A9GZW9
- SMR:   A9GZW9
- GeneID:   5788726
- GeneID:   6975039
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1623
- HOGENOM:   HBG541613
- OMA:   ARWEIFT
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27199; Mature: 27199

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLRVIPCLDVKNGRVVKGVNFVSLRDAGDPVEQAAVYDAAGADELTFLDITASHENRD
CEEEEEEEEEECCCCEEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TILDVVSRTAERIFLPLTVGGGVRTTDDMRRLLLAGADKCAMNSAAVARPDLVSEAARKF
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHH
GSQCVVVAVDARSDGHGSWEVYTHGGRTPTGRNVIDWCREVVERGAGEILLTSMDRDGTG
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
SGFDLDLLCAACTAVRVPIVASGGVGTLEHFVEGARAGATGLLAASVFHFGQFTIPQVKQ
CCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHH
ALADAGLPVRHTPAHPVP
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKLRVIPCLDVKNGRVVKGVNFVSLRDAGDPVEQAAVYDAAGADELTFLDITASHENRD
CEEEEEEEEEECCCCEEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TILDVVSRTAERIFLPLTVGGGVRTTDDMRRLLLAGADKCAMNSAAVARPDLVSEAARKF
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHH
GSQCVVVAVDARSDGHGSWEVYTHGGRTPTGRNVIDWCREVVERGAGEILLTSMDRDGTG
CCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
SGFDLDLLCAACTAVRVPIVASGGVGTLEHFVEGARAGATGLLAASVFHFGQFTIPQVKQ
CCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHH
ALADAGLPVRHTPAHPVP
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA