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The map label for this gene is gltX

Identifier: 162145889

GI number: 162145889

Start: 56352

End: 57752

Strand: Reverse

Name: gltX

Synonym: GDI_0050

Alternate gene names: 162145889

Gene position: 57752-56352 (Counterclockwise)

Preceding gene: 162145891

Following gene: 162145888

Centisome position: 1.46

GC content: 70.31

Gene sequence:

>1401_bases
ATGACCGTTCGCACGCGCTTCGCACCGAGCCCGACCGGGTTGCTGCATATCGGCAACGCCCGCGCCGCCCTGTTCAACTT
CCTGTACGCCCGTCACCATGGCGGCAAGTTCCTGCTTCGTATCGAGGATACGGACCGCGAACGCTCGACGCAGCAGGCGG
TGGACGTGCTGTTCGACGGGCTGGCCTGGATGGGCATCACCCCGGACGAGGAGCCGGTGTTCCAGTCCACGCGCCAGGCG
CGGCACGCCGAAATCGCGCACCACCTGCTGGCGCAGGGGCTGGCCTATCGCTGCTACTGCACGCCGGAAGAACTGCAGCA
GATGCGCGACCAGGCGGCGGCCGAGGGGCGGCCGCCCCGCTATAACGGCTACTGGCGCGACCGTGACCCGTCCGAGGCAC
CGGCCGGCGCGCCCTACACGGTGCGCATCCGTGCCCCGCGCGAGGGCGAGACGGTCATCCACGACCTGGTGCAGGGCGAT
GTCCGCGTGGCGAACGCCGAGCTGGACGACATGATCATCCTGCGCGGCGACGGCACCCCGGTGTACCAGTTGGCGGTGGT
GGTGGACGACCATGACATGGACATCACCCACGTCATTCGCGGCGACGACCACCTGACCAATACCTTCCGCCAGGCGATGA
TCTATCGCGCCATGGGGTGGGACCTGCCGGCGTTCGCGCATCTGCCCCTGATCCACGGGCCGGACGGCGCCAAGCTGTCC
AAGCGGCACGGCGCGCAGTCGGTCGTGGAATTCCGCGAGATGGGATACCTGCCCGAGGCACTGAACAACTACCTGCTGCG
GCTGGGCTGGGGCCATGGCGACGCCGAGATCCTGTCGCGGGACGAGCAGATCCAGCTGTTCGACCTGGACGGCGTCGGCC
GGTCGGCGTCCCGCATGGATTACGTGAAGCTGCAGCACCTGAACGGGGTATGGCTGCGCCAGGCGGACGATGCGCGGCTG
ACCGACGATATCGTGGCCCGGCTGGCGGACCGGCCGGACGTGAGCGTGGATGAGGCCGTGCGTGCGCGGATCCTGGCGCT
GATGCCCGGGCTGAAGGAACGGGCGAAGACGCTGGTGGACCTGGCCGACAGCGCCGCCTTCCTGGGCCGGCACGTGCCGC
TGGCGTTCGACGCCAAGGCCGAGAAGCTGCTGACGCCGGAAGCGCGGGCGATGCTGGGCGAACTGGCCCGCGACCTGGCG
GTGATCGAGCCGTTCGACGCGCCCGCGATCGACGTGGCGCTGCGCCGGTTCGCCGAACATCACGGGCACAAGCTGGGCCA
GGTGGCGCAGCCCCTGCGCGCGGCGATGACGGGCGGCGCGACCTCGCCGGGGATCGACGCCACGCTGGCGGCGCTGGGCC
GGGACGAGGTGATGGCCCGGATCGGGGCGGTCGCGCGATGA

Upstream 100 bases:

>100_bases
AAGACTTTTATTCGTTCCGGGGCATCGTGTGCCCCGGCGCACCAGCCAACCGATCCAGTCCCTTTTAAGCAGGCGGCATC
CCATGATATGGAGCCGCCTC

Downstream 100 bases:

>100_bases
CCGACGGTCCGCGCGCGGCGGCACCCCGCTTCTTCCCGCAGCATGCACGGCATCTGCTGGGCCTGCAGGGCATGCATCCG
ACCCGGATCGAACCGTTCCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTVRTRFAPSPTGLLHIGNARAALFNFLYARHHGGKFLLRIEDTDRERSTQQAVDVLFDGLAWMGITPDEEPVFQSTRQA
RHAEIAHHLLAQGLAYRCYCTPEELQQMRDQAAAEGRPPRYNGYWRDRDPSEAPAGAPYTVRIRAPREGETVIHDLVQGD
VRVANAELDDMIILRGDGTPVYQLAVVVDDHDMDITHVIRGDDHLTNTFRQAMIYRAMGWDLPAFAHLPLIHGPDGAKLS
KRHGAQSVVEFREMGYLPEALNNYLLRLGWGHGDAEILSRDEQIQLFDLDGVGRSASRMDYVKLQHLNGVWLRQADDARL
TDDIVARLADRPDVSVDEAVRARILALMPGLKERAKTLVDLADSAAFLGRHVPLAFDAKAEKLLTPEARAMLGELARDLA
VIEPFDAPAIDVALRRFAEHHGHKLGQVAQPLRAAMTGGATSPGIDATLAALGRDEVMARIGAVAR

Sequences:

>Translated_466_residues
MTVRTRFAPSPTGLLHIGNARAALFNFLYARHHGGKFLLRIEDTDRERSTQQAVDVLFDGLAWMGITPDEEPVFQSTRQA
RHAEIAHHLLAQGLAYRCYCTPEELQQMRDQAAAEGRPPRYNGYWRDRDPSEAPAGAPYTVRIRAPREGETVIHDLVQGD
VRVANAELDDMIILRGDGTPVYQLAVVVDDHDMDITHVIRGDDHLTNTFRQAMIYRAMGWDLPAFAHLPLIHGPDGAKLS
KRHGAQSVVEFREMGYLPEALNNYLLRLGWGHGDAEILSRDEQIQLFDLDGVGRSASRMDYVKLQHLNGVWLRQADDARL
TDDIVARLADRPDVSVDEAVRARILALMPGLKERAKTLVDLADSAAFLGRHVPLAFDAKAEKLLTPEARAMLGELARDLA
VIEPFDAPAIDVALRRFAEHHGHKLGQVAQPLRAAMTGGATSPGIDATLAALGRDEVMARIGAVAR
>Mature_465_residues
TVRTRFAPSPTGLLHIGNARAALFNFLYARHHGGKFLLRIEDTDRERSTQQAVDVLFDGLAWMGITPDEEPVFQSTRQAR
HAEIAHHLLAQGLAYRCYCTPEELQQMRDQAAAEGRPPRYNGYWRDRDPSEAPAGAPYTVRIRAPREGETVIHDLVQGDV
RVANAELDDMIILRGDGTPVYQLAVVVDDHDMDITHVIRGDDHLTNTFRQAMIYRAMGWDLPAFAHLPLIHGPDGAKLSK
RHGAQSVVEFREMGYLPEALNNYLLRLGWGHGDAEILSRDEQIQLFDLDGVGRSASRMDYVKLQHLNGVWLRQADDARLT
DDIVARLADRPDVSVDEAVRARILALMPGLKERAKTLVDLADSAAFLGRHVPLAFDAKAEKLLTPEARAMLGELARDLAV
IEPFDAPAIDVALRRFAEHHGHKLGQVAQPLRAAMTGGATSPGIDATLAALGRDEVMARIGAVAR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=507, Percent_Identity=34.1222879684418, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4826960, Length=305, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=43.3189655172414, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI226510925, Length=241, Percent_Identity=36.9294605809129, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1786895, Length=131, Percent_Identity=31.2977099236641, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=32.7044025157233, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324540, Length=488, Percent_Identity=32.5819672131148, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324742, Length=107, Percent_Identity=36.4485981308411, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=484, Percent_Identity=35.5371900826446, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17864488, Length=131, Percent_Identity=32.824427480916, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_GLUDA (A9GZS1)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600347.1
- RefSeq:   YP_002276032.1
- ProteinModelPortal:   A9GZS1
- SMR:   A9GZS1
- GeneID:   5788707
- GeneID:   6975056
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1640
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51608; Mature: 51477

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRTRFAPSPTGLLHIGNARAALFNFLYARHHGGKFLLRIEDTDRERSTQQAVDVLFDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
LAWMGITPDEEPVFQSTRQARHAEIAHHLLAQGLAYRCYCTPEELQQMRDQAAAEGRPPR
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YNGYWRDRDPSEAPAGAPYTVRIRAPREGETVIHDLVQGDVRVANAELDDMIILRGDGTP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCEEEEECCCCC
VYQLAVVVDDHDMDITHVIRGDDHLTNTFRQAMIYRAMGWDLPAFAHLPLIHGPDGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCHH
KRHGAQSVVEFREMGYLPEALNNYLLRLGWGHGDAEILSRDEQIQLFDLDGVGRSASRMD
HHCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCHHH
YVKLQHLNGVWLRQADDARLTDDIVARLADRPDVSVDEAVRARILALMPGLKERAKTLVD
HHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LADSAAFLGRHVPLAFDAKAEKLLTPEARAMLGELARDLAVIEPFDAPAIDVALRRFAEH
HHHHHHHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
HGHKLGQVAQPLRAAMTGGATSPGIDATLAALGRDEVMARIGAVAR
HCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHCCC
>Mature Secondary Structure 
TVRTRFAPSPTGLLHIGNARAALFNFLYARHHGGKFLLRIEDTDRERSTQQAVDVLFDG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
LAWMGITPDEEPVFQSTRQARHAEIAHHLLAQGLAYRCYCTPEELQQMRDQAAAEGRPPR
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YNGYWRDRDPSEAPAGAPYTVRIRAPREGETVIHDLVQGDVRVANAELDDMIILRGDGTP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEECCCCCCEEEEECCCCC
VYQLAVVVDDHDMDITHVIRGDDHLTNTFRQAMIYRAMGWDLPAFAHLPLIHGPDGAKLS
EEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCCCCCHH
KRHGAQSVVEFREMGYLPEALNNYLLRLGWGHGDAEILSRDEQIQLFDLDGVGRSASRMD
HHCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCHHHHCCCCEEEEEECCCCCCCCCHHH
YVKLQHLNGVWLRQADDARLTDDIVARLADRPDVSVDEAVRARILALMPGLKERAKTLVD
HHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
LADSAAFLGRHVPLAFDAKAEKLLTPEARAMLGELARDLAVIEPFDAPAIDVALRRFAEH
HHHHHHHHHCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
HGHKLGQVAQPLRAAMTGGATSPGIDATLAALGRDEVMARIGAVAR
HCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA