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The map label for this gene is pyrB

Identifier: 162145888

GI number: 162145888

Start: 55390

End: 56355

Strand: Reverse

Name: pyrB

Synonym: GDI_0049

Alternate gene names: 162145888

Gene position: 56355-55390 (Counterclockwise)

Preceding gene: 162145889

Following gene: 162145887

Centisome position: 1.43

GC content: 69.46

Gene sequence:

>966_bases
ATGACCGACGGTCCGCGCGCGGCGGCACCCCGCTTCTTCCCGCAGCATGCACGGCATCTGCTGGGCCTGCAGGGCATGCA
TCCGACCCGGATCGAACCGTTCCTGGACCTGGCCGAAAGCTATGCGCTGATGAGCCGCTCGCGCAAGACGCCGCGCGACC
GGCTGCTGGGGCGCACGGTCATCAACCTGTTCTTCGAGGACAGCACCCGGACGCGGACGTCGTTCGAACTGGCGGCGAAG
CGGCTGGGCGCCGACGTGATCAACATGACGGTGGCCAGTTCGTCGGTGAACAAGGGCGAGACTCTGCTGGACACGGCGGC
GACGCTGAACGCGATGCGGACCGACCTGCTGGTGGTGCGCCATTCGCAGTCCGGCGCGCCGGCGCTGCTCGCCCGCAAGG
TCGAGGCCAGCGTGGTGAATGCCGGCGACGGCACCCACGAACACCCCACCCAGGCGCTGCTGGACGCGCTGACCATCCGC
CGGCATTTCGGCACGCTGCACGGCCTGACGGTGGCGATCTGCGGCGATGTCAGCCACAGCCGCGTGGCACGGTCGAACAT
CCACCTGCTGACGGCGATGGGGGCCAGGGTACGGGTGGTGGGACCGCCCACGCTGATCCCCGGCGCGATCGGCGCCCTGG
GGGTGGATGTCCATTACACGATGGAAGACGGGCTGCGCGACGTGGACGTGGTGATGATGCTGCGCATGCAGCGCGAGCGC
ATGAGCGGCGGCCAGGTGCCCAGCGCGCGCGAATATTTCCGCTTCTACGGCCTGGACCGCAAGCGGCTGGCGGTCGCGCG
GCCCGGGGCGCTGGTCATGCATCCCGGCCCGATGAATCGCGGGGTCGAGATCGACAGCCGCGTCGCCGATTCCGACCAGA
GCGTCATCCGCGAGCAGGTCGAGATGGGGGTGGCCGTGCGCATGGCCGTCCTGGACCTGCTGTCGCGTGCCGGAGATCAG
TCGTGA

Upstream 100 bases:

>100_bases
GCGCGGCGATGACGGGCGGCGCGACCTCGCCGGGGATCGACGCCACGCTGGCGGCGCTGGGCCGGGACGAGGTGATGGCC
CGGATCGGGGCGGTCGCGCG

Downstream 100 bases:

>100_bases
CCGCTACCCTGTTTGAAAACGTCCGCCTGATCGACCCCGCGCGGGGCCTGGACCGGCCCGGGCGGCTGCTGGTGCGGGAC
GGCGTGATCGCCGGGACCGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTDGPRAAAPRFFPQHARHLLGLQGMHPTRIEPFLDLAESYALMSRSRKTPRDRLLGRTVINLFFEDSTRTRTSFELAAK
RLGADVINMTVASSSVNKGETLLDTAATLNAMRTDLLVVRHSQSGAPALLARKVEASVVNAGDGTHEHPTQALLDALTIR
RHFGTLHGLTVAICGDVSHSRVARSNIHLLTAMGARVRVVGPPTLIPGAIGALGVDVHYTMEDGLRDVDVVMMLRMQRER
MSGGQVPSAREYFRFYGLDRKRLAVARPGALVMHPGPMNRGVEIDSRVADSDQSVIREQVEMGVAVRMAVLDLLSRAGDQ
S

Sequences:

>Translated_321_residues
MTDGPRAAAPRFFPQHARHLLGLQGMHPTRIEPFLDLAESYALMSRSRKTPRDRLLGRTVINLFFEDSTRTRTSFELAAK
RLGADVINMTVASSSVNKGETLLDTAATLNAMRTDLLVVRHSQSGAPALLARKVEASVVNAGDGTHEHPTQALLDALTIR
RHFGTLHGLTVAICGDVSHSRVARSNIHLLTAMGARVRVVGPPTLIPGAIGALGVDVHYTMEDGLRDVDVVMMLRMQRER
MSGGQVPSAREYFRFYGLDRKRLAVARPGALVMHPGPMNRGVEIDSRVADSDQSVIREQVEMGVAVRMAVLDLLSRAGDQ
S
>Mature_320_residues
TDGPRAAAPRFFPQHARHLLGLQGMHPTRIEPFLDLAESYALMSRSRKTPRDRLLGRTVINLFFEDSTRTRTSFELAAKR
LGADVINMTVASSSVNKGETLLDTAATLNAMRTDLLVVRHSQSGAPALLARKVEASVVNAGDGTHEHPTQALLDALTIRR
HFGTLHGLTVAICGDVSHSRVARSNIHLLTAMGARVRVVGPPTLIPGAIGALGVDVHYTMEDGLRDVDVVMMLRMQRERM
SGGQVPSAREYFRFYGLDRKRLAVARPGALVMHPGPMNRGVEIDSRVADSDQSVIREQVEMGVAVRMAVLDLLSRAGDQS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=35.8552631578947, Blast_Score=171, Evalue=8e-43,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=32.1656050955414, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790703, Length=200, Percent_Identity=29.5, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786469, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=36.1194029850746, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322331, Length=312, Percent_Identity=34.6153846153846, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=35.5987055016181, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_GLUDA (A9GZR7)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600346.1
- RefSeq:   YP_002276033.1
- ProteinModelPortal:   A9GZR7
- SMR:   A9GZR7
- GeneID:   5788706
- GeneID:   6975057
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1641
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35026; Mature: 34895

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGPRAAAPRFFPQHARHLLGLQGMHPTRIEPFLDLAESYALMSRSRKTPRDRLLGRTV
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
INLFFEDSTRTRTSFELAAKRLGADVINMTVASSSVNKGETLLDTAATLNAMRTDLLVVR
HHHHCCCCCCCHHHHHHHHHHHCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEE
HSQSGAPALLARKVEASVVNAGDGTHEHPTQALLDALTIRRHFGTLHGLTVAICGDVSHS
ECCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
RVARSNIHLLTAMGARVRVVGPPTLIPGAIGALGVDVHYTMEDGLRDVDVVMMLRMQRER
HHHHCCEEEEEECCCEEEEECCCCCCCCCHHHEECEEEEEHHCCCCHHHHHHHHHHHHHH
MSGGQVPSAREYFRFYGLDRKRLAVARPGALVMHPGPMNRGVEIDSRVADSDQSVIREQV
CCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHH
EMGVAVRMAVLDLLSRAGDQS
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDGPRAAAPRFFPQHARHLLGLQGMHPTRIEPFLDLAESYALMSRSRKTPRDRLLGRTV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
INLFFEDSTRTRTSFELAAKRLGADVINMTVASSSVNKGETLLDTAATLNAMRTDLLVVR
HHHHCCCCCCCHHHHHHHHHHHCCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEE
HSQSGAPALLARKVEASVVNAGDGTHEHPTQALLDALTIRRHFGTLHGLTVAICGDVSHS
ECCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCHH
RVARSNIHLLTAMGARVRVVGPPTLIPGAIGALGVDVHYTMEDGLRDVDVVMMLRMQRER
HHHHCCEEEEEECCCEEEEECCCCCCCCCHHHEECEEEEEHHCCCCHHHHHHHHHHHHHH
MSGGQVPSAREYFRFYGLDRKRLAVARPGALVMHPGPMNRGVEIDSRVADSDQSVIREQV
CCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCCCCCCHHCCCHHHHHHHHH
EMGVAVRMAVLDLLSRAGDQS
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA