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The map label for this gene is ilvI [H]

Identifier: 162145864

GI number: 162145864

Start: 21930

End: 23756

Strand: Direct

Name: ilvI [H]

Synonym: GDI_0022

Alternate gene names: 162145864

Gene position: 21930-23756 (Clockwise)

Preceding gene: 162145863

Following gene: 162145865

Centisome position: 0.56

GC content: 68.31

Gene sequence:

>1827_bases
ATGAACGCGACACCGAACCTGAACGATCTGAAGCCGACCGCGCGCGCCCAGGCGGGGGACGTCGTCCTCTCCGGGGCCGA
GGTCCTGCTTCGCGTTCTCGCCGATCAGGGGGTCGAGGTCGTCTTCGGCTATCCGGGCGGCGCGGTGCTGCCGATCTATG
ACGCGCTGTTCCGGCAGAACGCCATCCGCCACGTGTTGGTCCGGCACGAGCAGGCGGCTGTCCACGCCGCCGAGGCCTAT
GCCCGCTCGACCGGCAAGGTCGGCGTGGTGCTGGTCACCAGCGGCCCCGGCGCCACCAACGCGGTGACCGGCCTGGTCGA
CGCGCTGATGGACTCGATCCCGGTCGTCTGCTTCTCGGGGCAGGTCGGCGTGCCGCTGATCGGCACCGATGCGTTCCAAG
AGGCCGACACGACGGGCATCACCCGTCCGGCGACCAAGCATAATTACCTGGTCCGGCGCCCGGGCGACCTGGCCCGCGTG
GTGCATGAAGCCTTCCACATCGCGCGCAGCGGCCGCCCCGGCCCGGTGCTGGTGGACCTGCCGAAGAACCTGACGGTGGG
CCCCGCGCCCTATCAGCCGGCCTCGCGCGCGCCGCATCCGTCCTATCGTCCGCGCACGGAACCGGACCGCGAGGCCGTGT
CCCGCGCGGTGGCGGCGATGAAGGGGGCGAAGCGGCCGCTGTTCTATACCGGCGGCGGCATCATCAATTCCGGCCCCGGC
GCCGGGGAATCGCTGGGCCGTCTGGTGCGCATGACCGGCTTTCCCTGCACCTCCACCCTGATGGGGCTGGGCGCGTTTCC
CACCACGGACCGCCAGTTCCTGGGCATGCTGGGCATGCACGGCACGTACGAGGCCAACCTGGCCACCCATGGGTGCGACG
TGCTGATCGCGCTGGGCGCGCGGTTCGACGACCGCGTCACCGGGCGGCTGGACGCGTTCTCGCCGGACTCGTTCAAGATC
CATGCGGATATCGACCCGTCGCAGATCAACAAGATCGTGCAGGTCGATGTGCCGGTGCTGGGCGATGCCGGCCGCGTCAT
CGACATGATGATCGAGGAATGGGAGCGCCAGAACGGAGCACCGGACAAGGCGGCGCTGGCCCAGTGGTGGCAGCAGATCG
ACGCCTGGCGCGGCATCCGCAGCCTGCATTTCACGCAGGACATGACGCCGTCGGCCATCATCCGCCCCCAGCATGCGGTG
CGGCGGCTGTATGAACTGGCCATGGAAACCGGCCGCGACACCTATGTCTCGACCGAGGTCGGGCAGCACCAGATGTGGGC
CGCCCAGCATTTCCAGTTCGACCGGCCGAACCGCTGGCTGACCTCGGGCGGGCTGGGCACGATGGGCTATGGCCTGCCGG
CGGCGGTGGGGGCGCAGATCGCCCATCCCGACGCGCTGGTGATCGACATCGCGGGCGAGGCCTCGACCCTGATGAACATC
CAGGAACTGGGGACCATCGCCCAGTATCGCCTGCCGGTGAAGCTGTTCATCCTGAACAACCAGTATATGGGCATGGTCCG
CCAGTGGCAGGAACTGCTGCATGGTTCGCGCTATTCCGAAAGCTACAGCGCGGCGCTGCCCGATTTCGTCCGCCTGGCCG
AAAGCTTCCATGCCAGGGGTATGCGCGCGACCTGCGTGGGTGAACTGGACGACGTGATCCGCGCCATGCTGGCCGAACCC
GGTGCGGTGGTGGCCGATATCTGCGTGGCGCAGGACGAAAACGTCTATCCGATGATTCCCTCCGGCGCGGCCCACAACCA
GATGCTGCTCGGCCCCGAACAGGATGGCGAGGTCACGGTCAGTGCCGAGGGACGCATGCTGGTCTGA

Upstream 100 bases:

>100_bases
CATTGACGTGGGCCGACAGTTTCCCTAAACCCAGCCGACATTCCGTCCGGGCGGGGCCTGTCGGGCCCAATCGGAACCAC
CGGACGGAGGAGCAGGGATC

Downstream 100 bases:

>100_bases
CCACGGCACCCTCGTCCCCCGGCCGGGCGGACCCCGGCCGCGTCTTTCATCGTTCACCGGCACGGGGCACGTTCCCCGCC
GGTCCCTTTCAGGAATGAAT

Product: acetolactate synthase isozyme III large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MNATPNLNDLKPTARAQAGDVVLSGAEVLLRVLADQGVEVVFGYPGGAVLPIYDALFRQNAIRHVLVRHEQAAVHAAEAY
ARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCFSGQVGVPLIGTDAFQEADTTGITRPATKHNYLVRRPGDLARV
VHEAFHIARSGRPGPVLVDLPKNLTVGPAPYQPASRAPHPSYRPRTEPDREAVSRAVAAMKGAKRPLFYTGGGIINSGPG
AGESLGRLVRMTGFPCTSTLMGLGAFPTTDRQFLGMLGMHGTYEANLATHGCDVLIALGARFDDRVTGRLDAFSPDSFKI
HADIDPSQINKIVQVDVPVLGDAGRVIDMMIEEWERQNGAPDKAALAQWWQQIDAWRGIRSLHFTQDMTPSAIIRPQHAV
RRLYELAMETGRDTYVSTEVGQHQMWAAQHFQFDRPNRWLTSGGLGTMGYGLPAAVGAQIAHPDALVIDIAGEASTLMNI
QELGTIAQYRLPVKLFILNNQYMGMVRQWQELLHGSRYSESYSAALPDFVRLAESFHARGMRATCVGELDDVIRAMLAEP
GAVVADICVAQDENVYPMIPSGAAHNQMLLGPEQDGEVTVSAEGRMLV

Sequences:

>Translated_608_residues
MNATPNLNDLKPTARAQAGDVVLSGAEVLLRVLADQGVEVVFGYPGGAVLPIYDALFRQNAIRHVLVRHEQAAVHAAEAY
ARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCFSGQVGVPLIGTDAFQEADTTGITRPATKHNYLVRRPGDLARV
VHEAFHIARSGRPGPVLVDLPKNLTVGPAPYQPASRAPHPSYRPRTEPDREAVSRAVAAMKGAKRPLFYTGGGIINSGPG
AGESLGRLVRMTGFPCTSTLMGLGAFPTTDRQFLGMLGMHGTYEANLATHGCDVLIALGARFDDRVTGRLDAFSPDSFKI
HADIDPSQINKIVQVDVPVLGDAGRVIDMMIEEWERQNGAPDKAALAQWWQQIDAWRGIRSLHFTQDMTPSAIIRPQHAV
RRLYELAMETGRDTYVSTEVGQHQMWAAQHFQFDRPNRWLTSGGLGTMGYGLPAAVGAQIAHPDALVIDIAGEASTLMNI
QELGTIAQYRLPVKLFILNNQYMGMVRQWQELLHGSRYSESYSAALPDFVRLAESFHARGMRATCVGELDDVIRAMLAEP
GAVVADICVAQDENVYPMIPSGAAHNQMLLGPEQDGEVTVSAEGRMLV
>Mature_608_residues
MNATPNLNDLKPTARAQAGDVVLSGAEVLLRVLADQGVEVVFGYPGGAVLPIYDALFRQNAIRHVLVRHEQAAVHAAEAY
ARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCFSGQVGVPLIGTDAFQEADTTGITRPATKHNYLVRRPGDLARV
VHEAFHIARSGRPGPVLVDLPKNLTVGPAPYQPASRAPHPSYRPRTEPDREAVSRAVAAMKGAKRPLFYTGGGIINSGPG
AGESLGRLVRMTGFPCTSTLMGLGAFPTTDRQFLGMLGMHGTYEANLATHGCDVLIALGARFDDRVTGRLDAFSPDSFKI
HADIDPSQINKIVQVDVPVLGDAGRVIDMMIEEWERQNGAPDKAALAQWWQQIDAWRGIRSLHFTQDMTPSAIIRPQHAV
RRLYELAMETGRDTYVSTEVGQHQMWAAQHFQFDRPNRWLTSGGLGTMGYGLPAAVGAQIAHPDALVIDIAGEASTLMNI
QELGTIAQYRLPVKLFILNNQYMGMVRQWQELLHGSRYSESYSAALPDFVRLAESFHARGMRATCVGELDDVIRAMLAEP
GAVVADICVAQDENVYPMIPSGAAHNQMLLGPEQDGEVTVSAEGRMLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=544, Percent_Identity=27.9411764705882, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI21361361, Length=585, Percent_Identity=26.8376068376068, Blast_Score=151, Evalue=2e-36,
Organism=Escherichia coli, GI87081685, Length=579, Percent_Identity=48.7046632124352, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1790104, Length=571, Percent_Identity=39.5796847635727, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=32.0224719101124, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=25.5855855855856, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.3597122302158, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=578, Percent_Identity=28.0276816608997, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=578, Percent_Identity=28.0276816608997, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=516, Percent_Identity=26.7441860465116, Blast_Score=115, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=600, Percent_Identity=43.1666666666667, Blast_Score=477, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=23.5416666666667, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323073, Length=560, Percent_Identity=22.1428571428571, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=535, Percent_Identity=20.7476635514019, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=540, Percent_Identity=22.7777777777778, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=555, Percent_Identity=27.3873873873874, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65601; Mature: 65601

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATPNLNDLKPTARAQAGDVVLSGAEVLLRVLADQGVEVVFGYPGGAVLPIYDALFRQN
CCCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
AIRHVLVRHEQAAVHAAEAYARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCFSG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECC
QVGVPLIGTDAFQEADTTGITRPATKHNYLVRRPGDLARVVHEAFHIARSGRPGPVLVDL
CCCCEEECCHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEC
PKNLTVGPAPYQPASRAPHPSYRPRTEPDREAVSRAVAAMKGAKRPLFYTGGGIINSGPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEECCCC
AGESLGRLVRMTGFPCTSTLMGLGAFPTTDRQFLGMLGMHGTYEANLATHGCDVLIALGA
CCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCCCCCCEEEEEECC
RFDDRVTGRLDAFSPDSFKIHADIDPSQINKIVQVDVPVLGDAGRVIDMMIEEWERQNGA
CCCCCCCCCCCCCCCCCEEEEECCCHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHCCC
PDKAALAQWWQQIDAWRGIRSLHFTQDMTPSAIIRPQHAVRRLYELAMETGRDTYVSTEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHHHHHHHHCCCCCEEECCC
GQHQMWAAQHFQFDRPNRWLTSGGLGTMGYGLPAAVGAQIAHPDALVIDIAGEASTLMNI
CCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCHHHHHHH
QELGTIAQYRLPVKLFILNNQYMGMVRQWQELLHGSRYSESYSAALPDFVRLAESFHARG
HHHHHHHHEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCC
MRATCVGELDDVIRAMLAEPGAVVADICVAQDENVYPMIPSGAAHNQMLLGPEQDGEVTV
CCEEHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCEECCCCCCCCCEEECCCCCCCEEE
SAEGRMLV
ECCCCCCC
>Mature Secondary Structure
MNATPNLNDLKPTARAQAGDVVLSGAEVLLRVLADQGVEVVFGYPGGAVLPIYDALFRQN
CCCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
AIRHVLVRHEQAAVHAAEAYARSTGKVGVVLVTSGPGATNAVTGLVDALMDSIPVVCFSG
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECC
QVGVPLIGTDAFQEADTTGITRPATKHNYLVRRPGDLARVVHEAFHIARSGRPGPVLVDL
CCCCEEECCHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEC
PKNLTVGPAPYQPASRAPHPSYRPRTEPDREAVSRAVAAMKGAKRPLFYTGGGIINSGPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEECCCC
AGESLGRLVRMTGFPCTSTLMGLGAFPTTDRQFLGMLGMHGTYEANLATHGCDVLIALGA
CCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCCCCCCEEEEEECC
RFDDRVTGRLDAFSPDSFKIHADIDPSQINKIVQVDVPVLGDAGRVIDMMIEEWERQNGA
CCCCCCCCCCCCCCCCCEEEEECCCHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHCCC
PDKAALAQWWQQIDAWRGIRSLHFTQDMTPSAIIRPQHAVRRLYELAMETGRDTYVSTEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCHHHHHHHHHHHHHCCCCCEEECCC
GQHQMWAAQHFQFDRPNRWLTSGGLGTMGYGLPAAVGAQIAHPDALVIDIAGEASTLMNI
CCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCHHHHHHH
QELGTIAQYRLPVKLFILNNQYMGMVRQWQELLHGSRYSESYSAALPDFVRLAESFHARG
HHHHHHHHEECCEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCC
MRATCVGELDDVIRAMLAEPGAVVADICVAQDENVYPMIPSGAAHNQMLLGPEQDGEVTV
CCEEHHHHHHHHHHHHHCCCCCCEEHHHEECCCCCCEECCCCCCCCCEEECCCCCCCEEE
SAEGRMLV
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]