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The map label for this gene is miaA

Identifier: 162145863

GI number: 162145863

Start: 20859

End: 21857

Strand: Direct

Name: miaA

Synonym: GDI_0021

Alternate gene names: 162145863

Gene position: 20859-21857 (Clockwise)

Preceding gene: 162145861

Following gene: 162145864

Centisome position: 0.53

GC content: 72.77

Gene sequence:

>999_bases
ATGACGCAAGCGGCGGATGCACGGGACCGGCCCGTGCTGGTGATCGCGGGGCCGACCTGCTCGGGCAAATCGGCCCTGGC
CATGGCGGTGGCGCGGGCGGTGGACGGCACGATCGTCAACGCGGATTCGATGCAGGTCTATCGCGACCTGCGCATCCTGA
CCGCCCGCCCCACCCCGGCGGACGAGGCCGAATGCCCGCACAGGCTGTACGGCGTCCTGCCCGCGACGGAAACCGGCAGC
GTGGCGTGGTGGCGTGGCCGCGCGGTGGCGGAAATCGAAGCCGCCTGGGCGGCGGGCCGCACCCCGATCCTGTGCGGGGG
CACCGGCATGTATCTGCGCGCCCTGACCGACGGGCTGACCGACATTCCCGACCCCGGGCCCGAGGCAAGGGCCGAGGCCC
GCCGGCTGGTCGATGCCGAAGGGCCCGCCGCCCTGCATGACCGCCTGGCCGCCGTCGATCCCGAAAGCGCCGCCGCGCTG
CACCCCGCCGACAGCCAGCGCGTCGCCCGCGCGTGGGAGGTCTGGTCGGGCACCGGGCACGGCATGGCCCATTGGCGCCG
CACGGCCACCCTGCCGCCTCTGGCCTGCCGGCGGGTCGCGGTCCGCCTGGACCCGCCGCGCGATACCCTGCGCGCGGCCA
TCGCCGTGCGTTTCGCCGCCATGGTCCAGGGCGGGGCACTGGACGAGGTCCGGGCCCTGCTGGCCCAGGGTCTGCCGCCC
GCCCTGCCGGCCATGCGCGCCCATGGCGTGCCGGAACTGGCCGCCCACCTGCGCGGCGACCTGACGCTGGACGAGGCCAC
GCACCGCGCCGTGCTGGCGACGGGGCGCTACACGCGGCGGCAATCGACGTGGTTTGCCCATCACGACCTGGCCGGACCCG
CCGACAGCCTGGTGATCGGCACCCGTATGGGTGGAGATGCGCAACAAATGGAAAGAAATTATGCGGATGCCGTTTCTTTT
ATTCTAATGCGCATTGACGTGGGCCGACAGTTTCCCTAA

Upstream 100 bases:

>100_bases
TGACATGGGCATCGGTCAGGGTCGTGGCGTCGCGGTTGGCGACGAGCGTAAGGATATGGGACAGCGACATGGCGCACAGG
CGTGTGGCAGGGATGACCGC

Downstream 100 bases:

>100_bases
ACCCAGCCGACATTCCGTCCGGGCGGGGCCTGTCGGGCCCAATCGGAACCACCGGACGGAGGAGCAGGGATCATGAACGC
GACACCGAACCTGAACGATC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTQAADARDRPVLVIAGPTCSGKSALAMAVARAVDGTIVNADSMQVYRDLRILTARPTPADEAECPHRLYGVLPATETGS
VAWWRGRAVAEIEAAWAAGRTPILCGGTGMYLRALTDGLTDIPDPGPEARAEARRLVDAEGPAALHDRLAAVDPESAAAL
HPADSQRVARAWEVWSGTGHGMAHWRRTATLPPLACRRVAVRLDPPRDTLRAAIAVRFAAMVQGGALDEVRALLAQGLPP
ALPAMRAHGVPELAAHLRGDLTLDEATHRAVLATGRYTRRQSTWFAHHDLAGPADSLVIGTRMGGDAQQMERNYADAVSF
ILMRIDVGRQFP

Sequences:

>Translated_332_residues
MTQAADARDRPVLVIAGPTCSGKSALAMAVARAVDGTIVNADSMQVYRDLRILTARPTPADEAECPHRLYGVLPATETGS
VAWWRGRAVAEIEAAWAAGRTPILCGGTGMYLRALTDGLTDIPDPGPEARAEARRLVDAEGPAALHDRLAAVDPESAAAL
HPADSQRVARAWEVWSGTGHGMAHWRRTATLPPLACRRVAVRLDPPRDTLRAAIAVRFAAMVQGGALDEVRALLAQGLPP
ALPAMRAHGVPELAAHLRGDLTLDEATHRAVLATGRYTRRQSTWFAHHDLAGPADSLVIGTRMGGDAQQMERNYADAVSF
ILMRIDVGRQFP
>Mature_331_residues
TQAADARDRPVLVIAGPTCSGKSALAMAVARAVDGTIVNADSMQVYRDLRILTARPTPADEAECPHRLYGVLPATETGSV
AWWRGRAVAEIEAAWAAGRTPILCGGTGMYLRALTDGLTDIPDPGPEARAEARRLVDAEGPAALHDRLAAVDPESAAALH
PADSQRVARAWEVWSGTGHGMAHWRRTATLPPLACRRVAVRLDPPRDTLRAAIAVRFAAMVQGGALDEVRALLAQGLPPA
LPAMRAHGVPELAAHLRGDLTLDEATHRAVLATGRYTRRQSTWFAHHDLAGPADSLVIGTRMGGDAQQMERNYADAVSFI
LMRIDVGRQFP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=29.3375394321767, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=34.8432055749129, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25144712, Length=183, Percent_Identity=28.9617486338798, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=25.6055363321799, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=32.8358208955224, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_GLUDA (A9GZI5)

Other databases:

- EMBL:   AM889285
- EMBL:   CP001189
- RefSeq:   YP_001600321.1
- RefSeq:   YP_002276156.1
- ProteinModelPortal:   A9GZI5
- SMR:   A9GZI5
- GeneID:   5791962
- GeneID:   6975199
- GenomeReviews:   AM889285_GR
- GenomeReviews:   CP001189_GR
- KEGG:   gdj:Gdia_1778
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35504; Mature: 35373

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAADARDRPVLVIAGPTCSGKSALAMAVARAVDGTIVNADSMQVYRDLRILTARPTPA
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEECCHHHHHHHHEEEEEECCCCC
DEAECPHRLYGVLPATETGSVAWWRGRAVAEIEAAWAAGRTPILCGGTGMYLRALTDGLT
CCCCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCC
DIPDPGPEARAEARRLVDAEGPAALHDRLAAVDPESAAALHPADSQRVARAWEVWSGTGH
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHCCCCC
GMAHWRRTATLPPLACRRVAVRLDPPRDTLRAAIAVRFAAMVQGGALDEVRALLAQGLPP
CHHHHHHCCCCCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
ALPAMRAHGVPELAAHLRGDLTLDEATHRAVLATGRYTRRQSTWFAHHDLAGPADSLVIG
CHHHHHHCCCHHHHHHHCCCCEEHHHHCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEE
TRMGGDAQQMERNYADAVSFILMRIDVGRQFP
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQAADARDRPVLVIAGPTCSGKSALAMAVARAVDGTIVNADSMQVYRDLRILTARPTPA
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEECCHHHHHHHHEEEEEECCCCC
DEAECPHRLYGVLPATETGSVAWWRGRAVAEIEAAWAAGRTPILCGGTGMYLRALTDGLT
CCCCCCHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEECCCCHHHHHHHHCCC
DIPDPGPEARAEARRLVDAEGPAALHDRLAAVDPESAAALHPADSQRVARAWEVWSGTGH
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHCCCCC
GMAHWRRTATLPPLACRRVAVRLDPPRDTLRAAIAVRFAAMVQGGALDEVRALLAQGLPP
CHHHHHHCCCCCHHHHHEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
ALPAMRAHGVPELAAHLRGDLTLDEATHRAVLATGRYTRRQSTWFAHHDLAGPADSLVIG
CHHHHHHCCCHHHHHHHCCCCEEHHHHCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEE
TRMGGDAQQMERNYADAVSFILMRIDVGRQFP
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA