Click here to switch to the map view.

The map label for this gene is yafV [C]

Identifier: 162145860

GI number: 162145860

Start: 17745

End: 18575

Strand: Reverse

Name: yafV [C]

Synonym: GDI_0018

Alternate gene names: 162145860

Gene position: 18575-17745 (Counterclockwise)

Preceding gene: 162145862

Following gene: 162145859

Centisome position: 0.47

GC content: 70.04

Gene sequence:

>831_bases
ATGGCCCCCGGCGCGTCCCTGCCGGACAATATCGCCCAGACCGGCCGCCTGATCGCCGAAGCGGTGGCCGCCGACCGGCC
CGACCTGGTGGTCCTGCCGGAAATATGGAGTTGCCTGGGCGGCACGGCCGAGGTGAAGTTCGCCAACGCCGAGGACCTGC
CCGCCCCCGGACAATCCATCCCGAATCCGCCCGGCGCGGGACCGCTCTATCGCTTCCTGAGCGAGGCCGCGCGCGCCCAC
GGCATTACCCTGCATGGCGGGTCCATCGGCCAGCGCCATGGCGACCGGCTGCTGAACACCACCCTGGTCTTCGGCCCGGA
CGGGGTGGAACACGCGCGCTACAGCAAGATCCATCTGTTCGACATCACCACGCCGGGGGGCGAGGGCTACCGCGAAAGCG
CAACCTATGCCCCGGGTGATACGGTGGTGACGGTGCCGGTGGGGCCGTTCACCGCCGGCCTGGCCATCTGCTACGATATC
CGCTTTGGCGAACTGTTCCTGGCCCTGCGCAAGCGGGGGGCGAACCTGATCGTGCTGCCGGCGGCCTTTACCGCCGAAAC
GGGCGAGGCCCATTGGGCCACGCTGCTGCGGGCCCGCGCGATCGAAACCCAGTGCTGGGTCGTGGCCTGCGGCACCACCG
GCACCCATATCGATGCCGGCGGCCATGCCCGGCGGACATATGGGCATTCCATGATCATCGATCCCTGGGGCACCGTCGTG
GCCCAGGCATCGAACGGGGTCGGGTGGGTCACGGCGCGCCTGGACCTGGACCACGTCGCCCGTGTCCGCGCCGGGATACC
GGTGATGGACCACCGCCGCCTGACGGCGTGA

Upstream 100 bases:

>100_bases
ACCAGCGCGCATTTGCAAACAGGCTCCAAGCGGGTGGGACAACCCTGCCATCGACCCACCTCCTCGGACGGAGTTTCCGG
TGCGTACCACAGTCATCCAG

Downstream 100 bases:

>100_bases
CCGGGATGCGCCCCGTCCGGCCCGCGTCCTCCATGAAAGCAGGCCCGCGATGAACGACGTTCATCCCCCTGTCTGGCCCT
CTGGCGGGGCGCCGCGCCGC

Product: Nitrilase/cyanide hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAPGASLPDNIAQTGRLIAEAVAADRPDLVVLPEIWSCLGGTAEVKFANAEDLPAPGQSIPNPPGAGPLYRFLSEAARAH
GITLHGGSIGQRHGDRLLNTTLVFGPDGVEHARYSKIHLFDITTPGGEGYRESATYAPGDTVVTVPVGPFTAGLAICYDI
RFGELFLALRKRGANLIVLPAAFTAETGEAHWATLLRARAIETQCWVVACGTTGTHIDAGGHARRTYGHSMIIDPWGTVV
AQASNGVGWVTARLDLDHVARVRAGIPVMDHRRLTA

Sequences:

>Translated_276_residues
MAPGASLPDNIAQTGRLIAEAVAADRPDLVVLPEIWSCLGGTAEVKFANAEDLPAPGQSIPNPPGAGPLYRFLSEAARAH
GITLHGGSIGQRHGDRLLNTTLVFGPDGVEHARYSKIHLFDITTPGGEGYRESATYAPGDTVVTVPVGPFTAGLAICYDI
RFGELFLALRKRGANLIVLPAAFTAETGEAHWATLLRARAIETQCWVVACGTTGTHIDAGGHARRTYGHSMIIDPWGTVV
AQASNGVGWVTARLDLDHVARVRAGIPVMDHRRLTA
>Mature_275_residues
APGASLPDNIAQTGRLIAEAVAADRPDLVVLPEIWSCLGGTAEVKFANAEDLPAPGQSIPNPPGAGPLYRFLSEAARAHG
ITLHGGSIGQRHGDRLLNTTLVFGPDGVEHARYSKIHLFDITTPGGEGYRESATYAPGDTVVTVPVGPFTAGLAICYDIR
FGELFLALRKRGANLIVLPAAFTAETGEAHWATLLRARAIETQCWVVACGTTGTHIDAGGHARRTYGHSMIIDPWGTVVA
QASNGVGWVTARLDLDHVARVRAGIPVMDHRRLTA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=265, Percent_Identity=37.7358490566038, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI297632350, Length=245, Percent_Identity=41.2244897959184, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI297632348, Length=245, Percent_Identity=41.2244897959184, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI5031947, Length=218, Percent_Identity=42.6605504587156, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI297632346, Length=137, Percent_Identity=36.4963503649635, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556280, Length=283, Percent_Identity=40.6360424028269, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=32.7272727272727, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=32, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=36.5671641791045, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355835, Length=266, Percent_Identity=35.7142857142857, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29207; Mature: 29075

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPGASLPDNIAQTGRLIAEAVAADRPDLVVLPEIWSCLGGTAEVKFANAEDLPAPGQSI
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCCEEEEECCCCCCCCCCCCC
PNPPGAGPLYRFLSEAARAHGITLHGGSIGQRHGDRLLNTTLVFGPDGVEHARYSKIHLF
CCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHCCCEEEEEEEECCCCCCCCCCCEEEEE
DITTPGGEGYRESATYAPGDTVVTVPVGPFTAGLAICYDIRFGELFLALRKRGANLIVLP
EEECCCCCCCCCCCCCCCCCEEEEEECCCHHCCEEEEEECHHHHHHHHHHHCCCCEEEEC
AAFTAETGEAHWATLLRARAIETQCWVVACGTTGTHIDAGGHARRTYGHSMIIDPWGTVV
CEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCCCHHHCCCEEEECCHHHEE
AQASNGVGWVTARLDLDHVARVRAGIPVMDHRRLTA
EECCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
APGASLPDNIAQTGRLIAEAVAADRPDLVVLPEIWSCLGGTAEVKFANAEDLPAPGQSI
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEECHHHHHHCCCCEEEEECCCCCCCCCCCCC
PNPPGAGPLYRFLSEAARAHGITLHGGSIGQRHGDRLLNTTLVFGPDGVEHARYSKIHLF
CCCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHCCCEEEEEEEECCCCCCCCCCCEEEEE
DITTPGGEGYRESATYAPGDTVVTVPVGPFTAGLAICYDIRFGELFLALRKRGANLIVLP
EEECCCCCCCCCCCCCCCCCEEEEEECCCHHCCEEEEEECHHHHHHHHHHHCCCCEEEEC
AAFTAETGEAHWATLLRARAIETQCWVVACGTTGTHIDAGGHARRTYGHSMIIDPWGTVV
CEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCCCHHHCCCEEEECCHHHEE
AQASNGVGWVTARLDLDHVARVRAGIPVMDHRRLTA
EECCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]