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The map label for this gene is trpA [H]

Identifier: 161899031

GI number: 161899031

Start: 237554

End: 238357

Strand: Reverse

Name: trpA [H]

Synonym: TM1040_0222

Alternate gene names: 161899031

Gene position: 238357-237554 (Counterclockwise)

Preceding gene: 99080064

Following gene: 99080060

Centisome position: 7.45

GC content: 62.44

Gene sequence:

>804_bases
ATGACCCGCCCAACCACACGCATCGACGCTAAGTTTGCTGAACTCGCTGCCGCGGGCAAAAAGGCCTTCGTCACCTATGT
CATGGCGGGCGATCCCGACTACGACACCTCTTTGGAGGTGGTCAAAGGCCTGCCCGCCGCTGGCGTCGATGTGATCGAGC
TCGGCTTGCCCTTTACCGATCCGATGGCGGACGGCGAAACCATCCAGCTCGCAGGTCAGCGCGCGCTGGAGGCCGGAATG
ACGCTGGAGCGCACACTGCAGCTGGCGCGGGATTTTCGCAAGGAGGATGACACCACGCCAATCGTGCTGATGGGCTACTA
CAACCCGATCTACTCGCGCGGTGTCGAGACATTCCTCAGCGATGCAAAAGAGGCCGGCATCGACGGTTTGATCGTGGTGG
ATCTGCCGCCAGAAGAAGACAGCGAACTCTGCCTGCCTGCACAAGCGGCGGGGCTGAACTTCATTCGTCTTGCGACCCCC
ACCACCGATGATGCTCGTCTGCCACGCGTGCTGCAAAACACATCCGGTTTTGTCTACTATGTGTCGATCACAGGGATCAC
CGGCGCCGCCGAGGCAGAGGCCGCGGACGTCGGGCCCGAAGTGGCTCGCATCAAGGCGGCCACGGATCTGCCGATCATCG
TGGGGTTCGGGATCAAAACGCCGGAACGGGCGCAGACCATCGCTTCGATCTCGGATGGGGCCGTCGTTGGCAGCGCCATC
GTGGCCGAGATCGGAGCCGGGAAATCACCTGCCGAGGTGCTGGCCTTTGTAAAAACACTGGCGGATGGCGCCCACGCGGG
CTGA

Upstream 100 bases:

>100_bases
AAACTGCCCGCTGCGCTGGCCCGACCTGTCGTCCGGGATTGATTTTACCGTGAAGTTTCGGCACATCAGGCAGGCAACAG
AGATCGAAAAAGGCCTTCAT

Downstream 100 bases:

>100_bases
TCCCAATTGAGACACCGCCCCAGGGGGCGCACATGGAACCAGCATCGGAGCGATCCTCCCTGCTGGTTTTTATTTGCTGA
GGCGGCGCGCGGCATAGCTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTRPTTRIDAKFAELAAAGKKAFVTYVMAGDPDYDTSLEVVKGLPAAGVDVIELGLPFTDPMADGETIQLAGQRALEAGM
TLERTLQLARDFRKEDDTTPIVLMGYYNPIYSRGVETFLSDAKEAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATP
TTDDARLPRVLQNTSGFVYYVSITGITGAAEAEAADVGPEVARIKAATDLPIIVGFGIKTPERAQTIASISDGAVVGSAI
VAEIGAGKSPAEVLAFVKTLADGAHAG

Sequences:

>Translated_267_residues
MTRPTTRIDAKFAELAAAGKKAFVTYVMAGDPDYDTSLEVVKGLPAAGVDVIELGLPFTDPMADGETIQLAGQRALEAGM
TLERTLQLARDFRKEDDTTPIVLMGYYNPIYSRGVETFLSDAKEAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATP
TTDDARLPRVLQNTSGFVYYVSITGITGAAEAEAADVGPEVARIKAATDLPIIVGFGIKTPERAQTIASISDGAVVGSAI
VAEIGAGKSPAEVLAFVKTLADGAHAG
>Mature_266_residues
TRPTTRIDAKFAELAAAGKKAFVTYVMAGDPDYDTSLEVVKGLPAAGVDVIELGLPFTDPMADGETIQLAGQRALEAGMT
LERTLQLARDFRKEDDTTPIVLMGYYNPIYSRGVETFLSDAKEAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPT
TDDARLPRVLQNTSGFVYYVSITGITGAAEAEAADVGPEVARIKAATDLPIIVGFGIKTPERAQTIASISDGAVVGSAIV
AEIGAGKSPAEVLAFVKTLADGAHAG

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=256, Percent_Identity=33.203125, Blast_Score=140, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=264, Percent_Identity=40.9090909090909, Blast_Score=198, Evalue=7e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 27914; Mature: 27783

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPTTRIDAKFAELAAAGKKAFVTYVMAGDPDYDTSLEVVKGLPAAGVDVIELGLPFTD
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEECCCCCC
PMADGETIQLAGQRALEAGMTLERTLQLARDFRKEDDTTPIVLMGYYNPIYSRGVETFLS
CCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
DAKEAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDDARLPRVLQNTSGFVYY
HHHHCCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEE
VSITGITGAAEAEAADVGPEVARIKAATDLPIIVGFGIKTPERAQTIASISDGAVVGSAI
EEEECCCCCCCCCHHHCCCHHHEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHH
VAEIGAGKSPAEVLAFVKTLADGAHAG
HHHHCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TRPTTRIDAKFAELAAAGKKAFVTYVMAGDPDYDTSLEVVKGLPAAGVDVIELGLPFTD
CCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCCEEEEECCCCCC
PMADGETIQLAGQRALEAGMTLERTLQLARDFRKEDDTTPIVLMGYYNPIYSRGVETFLS
CCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
DAKEAGIDGLIVVDLPPEEDSELCLPAQAAGLNFIRLATPTTDDARLPRVLQNTSGFVYY
HHHHCCCCEEEEEECCCCCCCCEECCHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEE
VSITGITGAAEAEAADVGPEVARIKAATDLPIIVGFGIKTPERAQTIASISDGAVVGSAI
EEEECCCCCCCCCHHHCCCHHHEEEECCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHH
VAEIGAGKSPAEVLAFVKTLADGAHAG
HHHHCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA